FastQCFastQC Report
Thu 26 May 2016
SRR936759_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936759_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1636159
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119680.7314692520714674No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101180.6183995565223185No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62910.3844980836214573No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27640.16893223702586363No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT24540.14998542317708732No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18370.11227515174258736No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17800.10879138274458655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110100.032.5376431
GTATCAA160850.029.419841
ATCAACG196100.024.0521683
TCAACGC196650.023.9600124
TATCACG1201.7101245E-423.75232
CAACGCA198950.023.6830185
TATCAAC199100.023.668792
GAGTACT111100.023.38599412-13
GTACATG125900.023.0200441
AACGCAG204900.022.9938936
TACATGG129100.022.0780492
CGTATAG1302.937282E-421.928551
CGACCGT652.9460565E-421.91984218-19
GTACTTT118300.021.84221614-15
ACATGGG127400.021.7361053
CGCGACA550.002879425221.589780-81
TACCTGG11250.021.535422
AGTACTT113500.021.0710512-13
GTATTAC2509.469659E-920.905221
ACGCAGA227850.020.6778497
Produced by FastQC (version 0.11.5)