##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936759_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1636159 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.778492799293957 31.0 31.0 34.0 30.0 34.0 2 31.875622723708393 31.0 31.0 34.0 30.0 34.0 3 31.978962924752423 31.0 31.0 34.0 30.0 34.0 4 35.757194746965304 37.0 35.0 37.0 35.0 37.0 5 35.614536851247344 37.0 35.0 37.0 33.0 37.0 6 35.6752632231953 37.0 35.0 37.0 33.0 37.0 7 35.59603559311778 37.0 35.0 37.0 33.0 37.0 8 35.567064081180376 37.0 35.0 37.0 33.0 37.0 9 37.124891896203245 39.0 37.0 39.0 33.0 39.0 10-11 37.025994417412974 39.0 37.0 39.0 33.0 39.0 12-13 36.89245635662549 39.0 37.0 39.0 33.0 39.0 14-15 37.90062854526974 40.0 37.0 41.0 33.0 41.0 16-17 37.884317783296126 39.5 37.0 41.0 32.5 41.0 18-19 38.07485336082863 40.0 37.0 41.0 33.0 41.0 20-21 38.10828898658382 40.0 37.0 41.0 33.0 41.0 22-23 37.99591757280313 40.0 37.0 41.0 33.0 41.0 24-25 37.869616583718326 40.0 37.0 41.0 33.0 41.0 26-27 37.637425213564214 39.5 37.0 41.0 32.0 41.0 28-29 37.600740820421485 39.0 37.0 41.0 32.0 41.0 30-31 37.407275209805405 39.0 36.0 41.0 32.0 41.0 32-33 37.14133742503021 39.0 35.5 41.0 31.5 41.0 34-35 36.92052942287394 39.0 35.0 41.0 31.0 41.0 36-37 36.69152784050939 39.0 35.0 41.0 31.0 41.0 38-39 36.44677167683581 39.0 35.0 40.0 30.0 41.0 40-41 36.16758212374225 38.5 35.0 40.0 30.0 41.0 42-43 35.837046093930965 38.0 35.0 40.0 29.5 41.0 44-45 35.445340581202686 38.0 35.0 40.0 27.5 41.0 46-47 34.9274703742118 38.0 34.0 40.0 25.5 41.0 48-49 34.41293572323961 38.0 33.5 40.0 23.5 41.0 50-51 34.057249326012936 38.0 33.0 40.0 23.0 41.0 52-53 33.768780723633824 38.0 33.0 40.0 22.0 41.0 54-55 33.42080904117509 37.0 33.0 40.0 20.0 41.0 56-57 33.0663929972576 37.0 32.0 40.0 18.5 41.0 58-59 32.699186631617096 36.5 31.5 39.0 18.0 40.5 60-61 32.31390500556486 36.0 31.0 39.0 15.0 40.0 62-63 31.975691237832017 35.5 31.0 39.0 13.0 40.0 64-65 31.656853337603497 35.0 31.0 39.0 9.5 40.0 66-67 31.20108161859575 35.0 30.0 38.0 8.0 40.0 68-69 30.75602585078834 35.0 29.5 38.0 7.0 40.0 70-71 30.255364240272492 34.0 29.0 37.0 4.0 39.0 72-73 29.69546144353941 34.0 28.5 36.5 2.0 39.0 74-75 29.153564231838104 34.0 28.0 36.0 2.0 38.5 76-77 27.81254755803073 31.5 26.5 34.5 2.0 36.5 78-79 28.574585355090797 33.0 28.0 35.0 2.0 37.0 80-81 28.420508948091232 33.0 27.5 35.0 2.0 37.0 82-83 28.00609781812159 33.0 27.0 35.0 2.0 36.0 84-85 27.548282593562117 33.0 26.0 35.0 2.0 36.0 86-87 27.10642272541972 33.0 26.0 35.0 2.0 35.5 88-89 26.578570909062016 32.0 25.0 34.5 2.0 35.0 90-91 25.965256738495462 32.0 24.0 34.0 2.0 35.0 92-93 25.356102310350032 31.5 21.5 34.0 2.0 35.0 94-95 24.588201391185088 31.0 18.5 34.0 2.0 35.0 96-97 23.736255156130916 31.0 4.5 34.0 2.0 35.0 98-99 22.59348480190495 30.5 2.0 34.0 2.0 35.0 100-101 18.91130140774827 24.5 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3398868557371024 1101 2 0.4633046452362919 1101 3 0.4467774397495923 1101 4 0.2055348956196994 1101 5 0.2829316274801812 1101 6 0.3487701275675761 1101 7 0.29936993656479416 1101 8 0.2636596436407288 1101 9 0.46847276816664873 1101 10-11 0.4505120427134699 1101 12-13 0.45710906592909595 1101 14-15 0.45392232530585375 1101 16-17 0.46025821164189296 1101 18-19 0.46635418248647653 1101 20-21 0.3800422384426696 1101 22-23 0.35114655201000744 1101 24-25 0.42756316620536694 1101 26-27 0.2845990582128124 1101 28-29 0.36219415730552385 1101 30-31 0.46919859269925723 1101 32-33 0.3295935909677752 1101 34-35 0.542820510012568 1101 36-37 0.48074016619928983 1101 38-39 0.4405561492483798 1101 40-41 0.3761824870317554 1101 42-43 0.603164735959588 1101 44-45 0.6237957904055236 1101 46-47 0.6871156516325101 1101 48-49 0.528429180322739 1101 50-51 0.604568874725075 1101 52-53 0.6200540607999869 1101 54-55 0.6219764731255921 1101 56-57 0.6215375136036059 1101 58-59 0.694244535780534 1101 60-61 0.5901308545020854 1101 62-63 0.6651140210307673 1101 64-65 0.5800741199126485 1101 66-67 0.5175497911869833 1101 68-69 0.605800866432741 1101 70-71 0.697894806672295 1101 72-73 0.8085995430235826 1101 74-75 0.6633952094711439 1101 76-77 0.640210679655997 1101 78-79 0.7553622842648124 1101 80-81 0.8390820141855606 1101 82-83 0.6335255961797124 1101 84-85 0.6431609730579488 1101 86-87 0.755742229886561 1101 88-89 0.5888868542357137 1101 90-91 0.6743823275178009 1101 92-93 0.8761304733699831 1101 94-95 0.7285853087154095 1101 96-97 1.0814925600932774 1101 98-99 0.7919307101012798 1101 100-101 1.0276999555358977 1102 1 0.2888600529874985 1102 2 0.3583208494251977 1102 3 0.41135825377494584 1102 4 0.22022884336858084 1102 5 0.06809041778239333 1102 6 0.22201843593624915 1102 7 0.2622395022495354 1102 8 0.23467268760970939 1102 9 0.24893744948427354 1102 10-11 0.29537426679885925 1102 12-13 0.2969858818462896 1102 14-15 0.3617196842649122 1102 16-17 0.35741508861367066 1102 18-19 0.519376545879517 1102 20-21 0.31530180106392436 1102 22-23 0.22552619761391668 1102 24-25 0.25124761717089683 1102 26-27 0.3136241733619798 1102 28-29 0.46380006498195314 1102 30-31 0.49563050017145827 1102 32-33 0.4418604038214866 1102 34-35 0.4957047481469701 1102 36-37 0.7133581504304445 1102 38-39 0.5260750326373369 1102 40-41 0.5962805183437325 1102 42-43 0.6440664735430985 1102 44-45 0.6653354839501802 1102 46-47 0.565661627995361 1102 48-49 0.7156781228852083 1102 50-51 0.5824220749852032 1102 52-53 0.709638771355003 1102 54-55 0.6191854829770946 1102 56-57 0.5818805157050448 1102 58-59 0.4931540449252907 1102 60-61 0.5464037298185076 1102 62-63 0.41572537309968993 1102 64-65 0.39252366657157367 1102 66-67 0.6233607261928675 1102 68-69 0.4283593546867195 1102 70-71 0.183598573675944 1102 72-73 0.1943903773108957 1102 74-75 0.21700070868377708 1102 76-77 0.31955477278574307 1102 78-79 0.2592905236245997 1102 80-81 0.4268297125457927 1102 82-83 0.35909972967213477 1102 84-85 0.1727251529325109 1102 86-87 0.3446658740285997 1102 88-89 0.2679283616821131 1102 90-91 0.2380887065399584 1102 92-93 0.24245424542568372 1102 94-95 0.34440629408058143 1102 96-97 0.3863226445270449 1102 98-99 0.1667548288137617 1102 100-101 0.3652759378644639 1103 1 0.29543864674843334 1103 2 0.3279395167976915 1103 3 0.25283682661121176 1103 4 0.21084113835163265 1103 5 0.2280498665205073 1103 6 0.2617198656082351 1103 7 0.23437825679575042 1103 8 0.2246528480501908 1103 9 0.1714029364594225 1103 10-11 0.23987555828015417 1103 12-13 0.19737668973113642 1103 14-15 0.332158436343434 1103 16-17 0.35848211143913744 1103 18-19 0.25207338395605916 1103 20-21 0.3294862917274344 1103 22-23 0.33465678951157685 1103 24-25 0.3642727663225358 1103 26-27 0.2664745423831931 1103 28-29 0.32028101592865 1103 30-31 0.3867603679877263 1103 32-33 0.44450083318619704 1103 34-35 0.41445602213469357 1103 36-37 0.541406737575933 1103 38-39 0.5947774091589793 1103 40-41 0.5001992792518593 1103 42-43 0.6493858688168288 1103 44-45 0.6013796941555398 1103 46-47 0.6484178427737177 1103 48-49 0.6618452748080941 1103 50-51 0.506746781203212 1103 52-53 0.619976064149256 1103 54-55 0.4841463328904325 1103 56-57 0.42628313335051615 1103 58-59 0.3032669130549266 1103 60-61 0.21346438194472483 1103 62-63 0.42161183024249027 1103 64-65 0.41930149426657337 1103 66-67 0.1467868886527981 1103 68-69 0.3377807284739589 1103 70-71 0.19895623168169152 1103 72-73 0.5834553286280837 1103 74-75 0.4013211691211147 1103 76-77 0.15977178085577037 1103 78-79 0.11431063530621088 1103 80-81 0.3222275026439938 1103 82-83 0.29215701965388163 1103 84-85 0.25165678470839126 1103 86-87 0.4539027794629611 1103 88-89 0.3130416405301837 1103 90-91 0.23640695822483693 1103 92-93 0.3607095473273958 1103 94-95 0.2651246220272476 1103 96-97 0.47658300620941674 1103 98-99 0.5803662066946629 1103 100-101 0.4905753144175442 1104 1 0.24810987511215288 1104 2 0.26670136280081636 1104 3 0.16909101709634555 1104 4 0.13877511021606637 1104 5 0.13067720169475905 1104 6 0.16314321625259254 1104 7 0.0753072083974331 1104 8 0.1446221527674254 1104 9 0.14759512407069764 1104 10-11 0.22415520748072026 1104 12-13 0.19617819600961894 1104 14-15 0.23774664834321868 1104 16-17 0.3704668822661077 1104 18-19 0.10455349891029897 1104 20-21 0.3056022735738253 1104 22-23 0.18943422541720167 1104 24-25 0.18739036745824222 1104 26-27 0.2783638064218579 1104 28-29 0.4229895817894018 1104 30-31 0.4439903982504063 1104 32-33 0.547985599687081 1104 34-35 0.5238475673546574 1104 36-37 0.4616995008235918 1104 38-39 0.7251069042508647 1104 40-41 0.6564074534349373 1104 42-43 0.6298019855707153 1104 44-45 0.6238672167094492 1104 46-47 0.6766598989050792 1104 48-49 0.8841438137206197 1104 50-51 0.9205038185892889 1104 52-53 0.9813669291745271 1104 54-55 0.9109826422419118 1104 56-57 0.902669329524791 1104 58-59 1.0158004652219361 1104 60-61 0.8674474476261125 1104 62-63 0.8781495860392852 1104 64-65 0.7324216644350408 1104 66-67 0.9545676636354727 1104 68-69 0.9176290623630301 1104 70-71 0.7217961643011535 1104 72-73 0.5230599305245107 1104 74-75 0.773008131812233 1104 76-77 0.6895218126359026 1104 78-79 0.8186740309818497 1104 80-81 0.882380639626021 1104 82-83 0.6928253009509895 1104 84-85 0.8238938598446133 1104 86-87 0.6586199163092168 1104 88-89 0.48125005963659717 1104 90-91 0.6535644698836514 1104 92-93 0.5520936703055312 1104 94-95 0.9146270216769032 1104 96-97 0.9055838587822116 1104 98-99 0.9646940086209028 1104 100-101 0.761167676137358 1105 1 0.1383463618637144 1105 2 0.195969148346407 1105 3 0.20143532736947023 1105 4 0.055604587104859604 1105 5 0.12534565731230174 1105 6 0.16994593734102637 1105 7 0.18125743162398322 1105 8 0.21049752252316978 1105 9 0.20725035871357278 1105 10-11 0.1896874988286399 1105 12-13 0.17474413218801743 1105 14-15 0.3330023977017831 1105 16-17 0.23162801338521177 1105 18-19 0.25786482344010864 1105 20-21 0.14759201064512695 1105 22-23 0.164705706558685 1105 24-25 0.11306161634995249 1105 26-27 0.21409103849116207 1105 28-29 0.20803399171023784 1105 30-31 0.2945512108107238 1105 32-33 0.45766417700035333 1105 34-35 0.2864702634919425 1105 36-37 0.3622214743188721 1105 38-39 0.34150738601219643 1105 40-41 0.2923275077311729 1105 42-43 0.33549014318669634 1105 44-45 0.34899550331820706 1105 46-47 0.6255286620966345 1105 48-49 0.7068768284951545 1105 50-51 0.7188192231824999 1105 52-53 0.6172575599366397 1105 54-55 0.6500527801009781 1105 56-57 0.9042670274274869 1105 58-59 0.6482377538628796 1105 60-61 0.5049642190405663 1105 62-63 0.7550758467396435 1105 64-65 0.553066589071566 1105 66-67 0.5214738378698769 1105 68-69 0.5720025764745564 1105 70-71 0.45731095845410863 1105 72-73 0.4817012301816277 1105 74-75 0.717172071898073 1105 76-77 0.30471691028280645 1105 78-79 0.3857675350501424 1105 80-81 0.7694903854851489 1105 82-83 0.7921699407676996 1105 84-85 0.6952688804410876 1105 86-87 0.8909010640624331 1105 88-89 0.9836059039351035 1105 90-91 0.8891194761999017 1105 92-93 0.5260509002563367 1105 94-95 0.5564239365212735 1105 96-97 0.6537439071152704 1105 98-99 0.8908291685672367 1105 100-101 0.6920616023352899 1106 1 0.07768122367244601 1106 2 0.19973880107076525 1106 3 0.10190467992941521 1106 4 0.15577628919293574 1106 5 0.10557854869501426 1106 6 0.0010959139613859747 1106 7 0.11083443611387622 1106 8 -0.002563903081806984 1106 9 0.004509912941770722 1106 10-11 0.02485600821317746 1106 12-13 0.19180498461472695 1106 14-15 0.12100939651017484 1106 16-17 0.17139401586899083 1106 18-19 0.07315812479613015 1106 20-21 0.11526400183628027 1106 22-23 0.13929606006890083 1106 24-25 0.254128834072219 1106 26-27 0.1125027313279432 1106 28-29 0.16416259984232795 1106 30-31 0.06764614723318374 1106 32-33 0.07500168850162936 1106 34-35 0.18282115418080735 1106 36-37 0.26087639083093705 1106 38-39 0.2729289938275343 1106 40-41 0.08163672735398109 1106 42-43 0.2133824309221879 1106 44-45 0.3192449112853879 1106 46-47 0.2820057022844793 1106 48-49 0.15439644979527145 1106 50-51 -0.11920449332553318 1106 52-53 -0.09744358290038946 1106 54-55 -0.1186666441062485 1106 56-57 -0.05358928582952416 1106 58-59 -0.050194427583242884 1106 60-61 -0.05484429416516434 1106 62-63 0.050597011598789265 1106 64-65 0.05509785242687215 1106 66-67 -0.0693315530226215 1106 68-69 -0.14173824136033986 1106 70-71 -0.06439439774602107 1106 72-73 0.10534056026695282 1106 74-75 -0.05911745352037201 1106 76-77 -0.04081280197164716 1106 78-79 -0.3216676932910083 1106 80-81 -0.21258773770795614 1106 82-83 -0.1846307943082941 1106 84-85 -0.13476490690977627 1106 86-87 -0.09291230607497525 1106 88-89 -0.07758401918861679 1106 90-91 0.07727718569907793 1106 92-93 -0.11015247244529647 1106 94-95 -0.10691615931011356 1106 96-97 -0.1952512711482015 1106 98-99 0.10094991309564705 1106 100-101 -0.17976392018709575 1107 1 0.08668928877807147 1107 2 0.1783431235775268 1107 3 0.10408664430650205 1107 4 0.18076593369477933 1107 5 0.08220070702449078 1107 6 0.14519449830387288 1107 7 0.09474077367875111 1107 8 0.13988876620013002 1107 9 0.16502988077881042 1107 10-11 0.1832597995437908 1107 12-13 0.14281495025883117 1107 14-15 0.22404112088335637 1107 16-17 0.25188791221654583 1107 18-19 0.32264765965151554 1107 20-21 0.15773068149808012 1107 22-23 0.21213724327593297 1107 24-25 0.34689643232761824 1107 26-27 0.22421662718817714 1107 28-29 0.236714438247823 1107 30-31 0.3093471291780645 1107 32-33 0.3203277039495944 1107 34-35 0.3043404885050265 1107 36-37 0.3658713958259412 1107 38-39 0.49546255782451 1107 40-41 0.5231403456868691 1107 42-43 0.5453279244530549 1107 44-45 0.4854031432972761 1107 46-47 0.5662970207221392 1107 48-49 0.46212713374546865 1107 50-51 0.4891968614172839 1107 52-53 0.6813514026019121 1107 54-55 0.605669385717583 1107 56-57 0.5456066401956647 1107 58-59 0.6647430739396256 1107 60-61 0.6211991745612337 1107 62-63 0.37456389837055326 1107 64-65 0.43780843452769247 1107 66-67 0.5142001591676255 1107 68-69 0.5252861976153191 1107 70-71 0.30559719602605995 1107 72-73 0.5116811708758533 1107 74-75 0.38673425574597076 1107 76-77 0.2128104040905825 1107 78-79 0.2460980775235413 1107 80-81 0.5241956009046511 1107 82-83 0.5369457998292724 1107 84-85 0.5453604555326628 1107 86-87 0.5817697192168048 1107 88-89 0.4309458655607763 1107 90-91 0.42654316362358813 1107 92-93 0.43643813207214066 1107 94-95 0.6803075925477877 1107 96-97 0.7401602685316284 1107 98-99 1.1124670544908355 1107 100-101 0.6073459211910368 1108 1 -0.003890948105667036 1108 2 0.12986319883300723 1108 3 -0.10469815066506172 1108 4 0.023223281794145123 1108 5 0.10258168791131084 1108 6 0.03409003856128123 1108 7 0.027192563134434522 1108 8 0.0556517969437067 1108 9 -0.0224054465397856 1108 10-11 -0.01448788079143526 1108 12-13 -0.013663007580994702 1108 14-15 0.02817696558341254 1108 16-17 0.06531096776022594 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-1.2601148499352597 2316 70-71 -1.140525839816604 2316 72-73 -0.9910746402911741 2316 74-75 -1.4275976740065168 2316 76-77 -1.2809316793711218 2316 78-79 -1.1504023846311249 2316 80-81 -1.277996210399312 2316 82-83 -1.2979528684806105 2316 84-85 -0.9880867047836581 2316 86-87 -1.4006940786675557 2316 88-89 -1.6035128999727526 2316 90-91 -2.079523900153756 2316 92-93 -1.8863084360015314 2316 94-95 -1.9579947593059686 2316 96-97 -1.640222121663843 2316 98-99 -1.05130001955661 2316 100-101 -0.7489205537670287 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 21.0 4 37.0 5 91.0 6 208.0 7 433.0 8 1043.0 9 1927.0 10 3141.0 11 4985.0 12 6953.0 13 9398.0 14 12013.0 15 15849.0 16 20255.0 17 25654.0 18 21995.0 19 14387.0 20 12596.0 21 13029.0 22 14060.0 23 15737.0 24 18283.0 25 21659.0 26 25691.0 27 31601.0 28 38362.0 29 46461.0 30 56500.0 31 67868.0 32 83912.0 33 104229.0 34 133943.0 35 175700.0 36 231974.0 37 269770.0 38 131566.0 39 4827.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.57218346555696 19.932444616137865 15.478324758510285 21.01704715979489 2 15.84032958535937 21.58307329790128 39.361244991580044 23.215352125159306 3 19.356863617582558 26.74680149332916 30.361792483967736 23.534542405120547 4 12.84122746541886 17.842963245468635 37.20925684166263 32.10655244744987 5 12.931107724900324 38.625296217040216 33.291525243467945 15.15207081459151 6 29.71997220319052 37.34661484611214 17.645167737365377 15.288245213331955 7 27.448699816765416 34.61446783126772 20.37503804654085 17.56179430542602 8 27.20934596232938 33.548027080994224 19.557993287815325 19.684633668861075 9 27.44128168472624 16.47889966684167 20.535229155601627 35.54458949283046 10-11 24.887770687323176 27.337593717969956 26.36409419866896 21.410541396037917 12-13 26.71763563321169 25.11299329710621 27.386335924564786 20.783035145117314 14-15 23.435232869277588 25.69626697731387 27.139713009900046 23.728787143508494 16-17 21.187364233909463 29.144311484848593 27.56202913538775 22.10629514585419 18-19 21.067083177223367 26.583719049734956 31.68370175285261 20.665496020189067 20-21 22.547110537407285 25.305564519677876 31.636730572709627 20.51059437020521 22-23 21.97970912893617 25.46663533303242 31.60147773330697 20.952177804724432 24-25 21.591052165143783 25.78920025670018 31.92418176817529 20.69556580998075 26-27 21.403196208189005 25.843875054815935 31.843969788972025 20.908958948023034 28-29 20.98162280170986 26.059769288785066 31.96017744007608 20.998430469428992 30-31 21.18120696257094 25.79674155493832 31.87287095272831 21.149180529762425 32-33 21.048599220160792 25.92224283688134 31.60790572007385 21.421252222884025 34-35 21.628169647659377 25.892525352291727 31.48677622507554 20.99252877497335 36-37 21.873541724666616 25.565501411404245 31.380847502638076 21.180109361291066 38-39 21.543635749638234 25.82119679904161 31.377979887810596 21.257187563509564 40-41 21.550784963681696 25.90341950367278 31.348215958294993 21.197579574350527 42-43 21.5306837493739 26.12344399259589 30.73450975586426 21.61136250216595 44-45 21.530149868419322 26.227722127771546 30.555811080037298 21.686316923771834 46-47 22.07257974316677 26.55643491861121 29.76960063172345 21.601384706498575 48-49 22.260208456719834 26.79355841476078 29.10103115971393 21.845201968805448 50-51 22.234476629836212 27.012931608792854 28.940754675927455 21.811837085443475 52-53 22.449626332589208 27.148163597719645 28.64717270295007 21.755037366741078 54-55 22.037687669012506 27.114090719205997 28.761730602505807 22.08649100927569 56-57 22.295593620661517 27.35067107017309 28.316306727083457 22.037428582081933 58-59 22.43839827884959 26.890102468347273 28.432964675465968 22.23853457733717 60-61 22.167304621543774 27.03391475089199 28.951049378471506 21.84773124909273 62-63 22.271748952650224 26.953002881453358 28.937413268376105 21.83783489752031 64-65 22.01327920434792 27.118126609017096 28.797830439853016 22.070763746781967 66-67 22.051755651520462 27.234716301664204 28.54558361939955 22.167944427415783 68-69 22.183872919633526 27.141975270913683 28.65136022198725 22.022791587465544 70-71 22.2369594031075 27.08304364076553 28.528913890482304 22.151083065644666 72-73 22.27677264423162 27.144534475561105 28.38290545632574 22.195787423881534 74-75 22.0503799292005 27.703276562724916 28.07771991747088 22.16862359060371 76-77 22.083286729485597 27.432002694160794 28.261612303660282 22.223098272693324 78-79 22.123946544224975 27.502347125138442 28.159358794751242 22.21434753588534 80-81 22.02861240559535 27.83345358819418 28.0032467284413 22.134687277769167 82-83 22.238089897289317 27.704410578690002 27.903848204923843 22.15365131909684 84-85 21.98552062195927 27.6335171014351 27.845176392929616 22.535785883676013 86-87 22.115501270821408 27.55263663736713 27.741495031160106 22.590367060651353 88-89 22.143532744639334 27.449449379090314 27.641582230996438 22.76543564527392 90-91 21.992960370068033 27.467392736106788 27.87631106765887 22.66333582616631 92-93 22.053773427202586 27.413146646105645 27.989729268681025 22.54335065801075 94-95 21.82817810187125 27.27115762671412 28.00189591013313 22.898768361281505 96-97 21.957832273379715 27.176556765810606 27.934247121225873 22.931363839583803 98-99 21.687802215634242 27.434876665919994 27.668046637661504 23.20927448078426 100-101 21.634736008620376 27.505930939809865 27.78644154918623 23.07289150238353 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1001.0 1 791.0 2 1006.0 3 1242.5 4 825.5 5 631.0 6 1027.0 7 2014.0 8 3180.0 9 3764.0 10 3291.0 11 2633.5 12 2511.5 13 2636.5 14 2868.5 15 3142.0 16 3379.0 17 3624.5 18 3926.0 19 4197.5 20 4492.5 21 5075.0 22 5893.0 23 6764.5 24 7722.0 25 9033.5 26 11146.5 27 13568.0 28 15563.0 29 18247.0 30 21574.5 31 25082.0 32 29726.5 33 33446.5 34 35978.0 35 38187.5 36 41437.5 37 44902.0 38 45694.0 39 45538.5 40 46108.5 41 48342.0 42 49814.5 43 50982.5 44 53104.0 45 55381.5 46 56039.5 47 56927.5 48 63245.5 49 62679.5 50 55533.0 51 51886.0 52 49575.5 53 49128.0 54 48235.5 55 44908.0 56 40930.0 57 37617.5 58 35891.0 59 33207.5 60 28125.0 61 23688.5 62 20435.0 63 16029.5 64 11721.5 65 9212.0 66 7066.0 67 5388.5 68 4001.5 69 2872.5 70 2252.0 71 1835.0 72 1594.5 73 1513.5 74 1278.5 75 1056.5 76 804.5 77 509.5 78 323.0 79 213.5 80 142.0 81 112.0 82 79.5 83 48.0 84 25.0 85 12.0 86 6.0 87 3.0 88 2.0 89 3.0 90 2.5 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010267950730949743 2 0.010023475713546177 3 0.00507285661112398 4 4.2783128045623933E-4 5 0.006845300487299829 6 0.0 7 3.055937717544566E-4 8 0.0018335626305267397 9 0.0 10-11 0.0 12-13 0.0 14-15 3.055937717544566E-4 16-17 0.004461669067615067 18-19 0.0015890876131231745 20-21 4.2783128045623933E-4 22-23 2.7503439457901097E-4 24-25 5.500687891580219E-4 26-27 9.1678131526337E-5 28-29 6.111875435089133E-5 30-31 6.417469206843589E-4 32-33 0.0032087346034217947 34-35 9.167813152633699E-4 36-37 0.0013751719728950548 38-39 0.0052867722513521 40-41 3.0559377175445665E-5 42-43 0.0024753095512110987 44-45 0.005531247268755665 46-47 0.0 48-49 0.002444750174035653 50-51 7.945438065615873E-4 52-53 7.028656750352502E-4 54-55 3.0559377175445665E-5 56-57 0.011612563326669352 58-59 0.0026892251914392186 60-61 0.00436999093608873 62-63 3.361531489299023E-4 64-65 0.004094956541509718 66-67 0.0017418844990004028 68-69 0.0017724438761758485 70-71 0.005225653497001209 72-73 0.003544887752351697 74-75 0.007975997442791318 76-77 0.0017113251218249573 78-79 0.007028656750352502 80-81 0.003086497094720012 82-83 0.0035754471295271427 84-85 0.0023836314196847615 86-87 7.639844293861417E-4 88-89 0.003147615849070903 90-91 0.008281591214545775 92-93 0.003269853357772686 94-95 3.361531489299023E-4 96-97 1.527968858772283E-4 98-99 8.251031837370328E-4 100-101 0.002169715779456642 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1636159.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.64710298455402 #Duplication Level Percentage of deduplicated Percentage of total 1 76.16451140893705 40.098408759145485 2 13.529912111714882 14.246213526348361 3 4.448598999955567 7.026175490629343 4 1.9489602056308146 4.104284346345723 5 1.0033752644705873 2.641240044036856 6 0.6017566478938594 1.9008446527984815 7 0.4030569519016946 1.4853846598786267 8 0.28683340431085025 1.2080758220930843 9 0.21869168714141116 1.0362135397319765 >10 1.217989788494529 12.273977154959038 >50 0.11191540917709306 4.134330345240084 >100 0.058575726136534896 5.600545664628602 >500 0.004308571734025972 1.507831837276234 >1k 0.0011644788470340465 0.9967813719160109 >5k 1.1644788470340466E-4 0.38567880009370964 >10k+ 2.328957694068093E-4 1.3540139848783455 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11968 0.7314692520714674 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10118 0.6183995565223185 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6291 0.3844980836214573 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2764 0.16893223702586363 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2454 0.14998542317708732 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1837 0.11227515174258736 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1780 0.10879138274458655 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 1.2223750870178266E-4 14-15 0.0 0.0 0.0 0.0 1.2223750870178266E-4 16-17 0.0 0.0 0.0 0.0 1.2223750870178266E-4 18-19 0.0 0.0 0.0 3.0559377175445665E-5 1.2223750870178266E-4 20-21 0.0 0.0 0.0 9.1678131526337E-5 1.2223750870178266E-4 22-23 0.0 0.0 0.0 1.2223750870178266E-4 1.2223750870178266E-4 24-25 0.0 0.0 0.0 3.055937717544566E-4 1.2223750870178266E-4 26-27 0.0 0.0 0.0 4.278312804562393E-4 1.83356263052674E-4 28-29 0.0 0.0 0.0 9.779000696142613E-4 1.83356263052674E-4 30-31 0.0 0.0 0.0 0.0019558001392285225 1.83356263052674E-4 32-33 0.0 0.0 0.0 0.0035143283751762513 1.83356263052674E-4 34-35 0.0 0.0 0.0 0.005195094119825763 1.83356263052674E-4 36-37 0.0 0.0 0.0 0.008434388100423003 1.83356263052674E-4 38-39 0.0 0.0 0.0 0.01310997280826619 1.83356263052674E-4 40-41 0.0 0.0 0.0 0.018060591910688387 1.83356263052674E-4 42-43 0.0 0.0 0.0 0.022369464092426225 1.83356263052674E-4 44-45 0.0 0.0 0.0 0.026067148730655153 1.83356263052674E-4 46-47 0.0 0.0 0.0 0.03153727724505992 1.83356263052674E-4 48-49 0.0 0.0 0.0 0.03667125261053479 2.444750174035653E-4 50-51 0.0 0.0 0.0 0.04162187171295699 2.444750174035653E-4 52-53 0.0 0.0 0.0 0.05329555379397724 3.055937717544566E-4 54-55 0.0 0.0 0.0 0.07181453636229732 3.055937717544566E-4 56-57 0.0 0.0 0.0 0.10219055727469029 3.055937717544566E-4 58-59 0.0 0.0 0.0 0.1404814568755237 3.055937717544566E-4 60-61 0.0 0.0 0.0 0.18702338831372745 3.055937717544566E-4 62-63 0.0 0.0 0.0 0.21721605296306778 3.66712526105348E-4 64-65 0.0 0.0 0.0 0.2521759804517776 3.66712526105348E-4 66-67 0.0 0.0 0.0 0.31577004435388 3.66712526105348E-4 68-69 0.0 0.0 0.0 0.4053395788551113 3.66712526105348E-4 70-71 0.0 0.0 0.0 0.4944201633215354 3.66712526105348E-4 72-73 0.0 0.0 0.0 0.59107947332747 3.66712526105348E-4 74-75 0.0 0.0 0.0 0.685630186308299 3.66712526105348E-4 76-77 0.0 0.0 0.0 0.7723576987322136 3.972719032807936E-4 78-79 0.0 0.0 0.0 0.855479204649426 4.278312804562393E-4 80-81 0.0 0.0 0.0 0.9349030259284091 4.278312804562393E-4 82-83 0.0 0.0 0.0 1.048920062169997 4.889500348071306E-4 84-85 0.0 0.0 0.0 1.2268978748397925 4.889500348071306E-4 86-87 0.0 0.0 0.0 1.4518760096054235 4.889500348071306E-4 88-89 0.0 0.0 0.0 1.719973425565608 4.889500348071306E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11010 0.0 32.537643 1 GTATCAA 16085 0.0 29.41984 1 ATCAACG 19610 0.0 24.052168 3 TCAACGC 19665 0.0 23.960012 4 TATCACG 120 1.7101245E-4 23.7523 2 CAACGCA 19895 0.0 23.683018 5 TATCAAC 19910 0.0 23.66879 2 GAGTACT 11110 0.0 23.385994 12-13 GTACATG 12590 0.0 23.020044 1 AACGCAG 20490 0.0 22.993893 6 TACATGG 12910 0.0 22.078049 2 CGTATAG 130 2.937282E-4 21.92855 1 CGACCGT 65 2.9460565E-4 21.919842 18-19 GTACTTT 11830 0.0 21.842216 14-15 ACATGGG 12740 0.0 21.736105 3 CGCGACA 55 0.0028794252 21.5897 80-81 TACCTGG 1125 0.0 21.53542 2 AGTACTT 11350 0.0 21.07105 12-13 GTATTAC 250 9.469659E-9 20.90522 1 ACGCAGA 22785 0.0 20.677849 7 >>END_MODULE