FastQCFastQC Report
Thu 26 May 2016
SRR936758_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936758_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1654826
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112360.6789837723120135No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87360.5279104872657306No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53400.32269253685886007No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21790.1316754752463401No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21730.131312899362229No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94400.032.5233841
GTATCAA144450.032.144911
GCGCGAC856.3920865E-427.9533429
ATCAACG175850.026.3764533
TATCAAC176000.026.3007642
TCAACGC176550.026.2726694
CAACGCA178250.026.0228885
AACGCAG182400.025.612436
GTACATG126350.025.314831
TACATGG128600.024.501612
GAGTACT91600.024.30286212-13
ACATGGG127100.023.9300373
TAGGACG1401.7728624E-523.7624954
ACGCAGA201250.023.1168947
CGCAGAG203050.022.865168
GTACTTT98350.022.63250214-15
CATGGGT13750.022.466364
GCAGAGT209250.021.8016039
AGTACTT93950.021.79835712-13
CATGGGG43750.021.6170834
Produced by FastQC (version 0.11.5)