FastQCFastQC Report
Thu 26 May 2016
SRR936758_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936758_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1654826
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120820.7301069719716756No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103840.6274979967682403No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64490.38970864610539113No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28180.17028980690416998No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT24600.14865611248554228No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19180.11590342428750817No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18760.11336539309873062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112650.033.1396831
TTACGCT650.0058456629.2298954
GTATCAA162950.029.0350421
GAGTACT116650.023.51018312-13
ATCAACG201950.023.333223
GTACATG119450.023.1385571
TCAACGC204700.023.0647724
TATCAAC204550.022.9929712
CAACGCA207150.022.7878485
TAGCCGC1050.002199324522.6142715
AACGCAG211150.022.356166
TACATGG122950.022.2949582
GTACTTT124050.022.05029914-15
ACATGGG122500.021.4435143
AGTACTT118700.020.94376412-13
ACGCAGA235250.019.9851517
CGCAGAG236500.019.8996018
ACTTTTT141000.019.56915316-17
GCAGAGT244650.019.0231639
CATGGGA75300.018.8606594
Produced by FastQC (version 0.11.5)