##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936758_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1654826 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.36953311103403 31.0 31.0 34.0 30.0 34.0 2 31.55481120069421 31.0 31.0 34.0 30.0 34.0 3 31.808893503002732 31.0 31.0 34.0 30.0 34.0 4 35.658954476180575 37.0 35.0 37.0 33.0 37.0 5 35.458562410791224 37.0 35.0 37.0 33.0 37.0 6 35.573926201304545 37.0 35.0 37.0 33.0 37.0 7 35.503839678612735 37.0 35.0 37.0 33.0 37.0 8 35.48621849064494 37.0 35.0 37.0 33.0 37.0 9 37.07184682860917 39.0 37.0 39.0 33.0 39.0 10-11 36.953328930050645 39.0 37.0 39.0 33.0 39.0 12-13 36.81917615507612 39.0 37.0 39.0 32.0 39.0 14-15 37.85168229167296 40.0 37.0 41.0 33.0 41.0 16-17 37.772055793177046 39.0 37.0 41.0 32.5 41.0 18-19 37.8893802127837 39.5 37.0 41.0 33.0 41.0 20-21 37.98292328015151 40.0 37.0 41.0 33.0 41.0 22-23 37.885991941146685 40.0 37.0 41.0 33.0 41.0 24-25 37.76762511587321 40.0 37.0 41.0 32.0 41.0 26-27 37.536910527149075 39.0 36.5 41.0 32.0 41.0 28-29 37.42067413734132 39.0 36.0 41.0 32.0 41.0 30-31 37.32391774120059 39.0 36.0 41.0 32.0 41.0 32-33 37.081859361648895 39.0 35.5 41.0 31.0 41.0 34-35 36.869648531023806 39.0 35.0 41.0 31.0 41.0 36-37 36.588549188857314 39.0 35.0 41.0 30.0 41.0 38-39 36.40518247839954 39.0 35.0 40.0 30.0 41.0 40-41 36.12109188518914 38.5 35.0 40.0 30.0 41.0 42-43 35.80688090469934 38.0 35.0 40.0 29.0 41.0 44-45 35.432459968600924 38.0 35.0 40.0 27.5 41.0 46-47 34.90967237643112 38.0 34.0 40.0 25.0 41.0 48-49 34.35920423053542 38.0 33.5 40.0 23.5 41.0 50-51 34.11630467493259 38.0 33.0 40.0 23.0 41.0 52-53 33.81747718491249 38.0 33.0 40.0 22.0 41.0 54-55 33.523888916417796 37.5 33.0 40.0 20.5 41.0 56-57 33.24114227115117 37.0 32.5 40.0 19.5 41.0 58-59 32.878502331967226 37.0 32.0 40.0 18.5 41.0 60-61 32.50543440820968 36.0 31.0 39.0 16.5 40.5 62-63 32.204615167999535 36.0 31.0 39.0 14.5 40.0 64-65 31.757903549980483 35.0 31.0 39.0 12.0 40.0 66-67 31.347727495217022 35.0 30.0 38.0 8.0 40.0 68-69 30.895353952621 35.0 30.0 38.0 7.5 40.0 70-71 30.4283383872383 34.0 29.0 37.0 7.0 39.0 72-73 30.02888883785969 34.0 29.0 36.5 3.5 39.0 74-75 29.668465445913952 34.0 29.0 36.0 2.0 39.0 76-77 28.267713644818247 32.5 27.0 34.5 2.0 37.0 78-79 29.057423862085802 34.0 29.0 35.0 2.0 37.0 80-81 28.905248648498393 34.0 29.0 35.0 2.0 37.0 82-83 28.56484820760612 34.0 28.5 35.0 2.0 36.5 84-85 28.10172005999422 33.0 27.0 35.0 2.0 36.0 86-87 27.703808738803957 33.0 26.5 35.0 2.0 36.0 88-89 27.266052442975877 33.0 26.0 35.0 2.0 35.0 90-91 26.78649144985636 33.0 25.0 35.0 2.0 35.0 92-93 26.310070968186384 32.0 25.0 34.0 2.0 35.0 94-95 25.753267715155552 32.0 23.5 34.0 2.0 35.0 96-97 25.052043538112166 32.0 19.5 34.0 2.0 35.0 98-99 24.157171509270462 31.0 8.5 34.0 2.0 35.0 100-101 21.38725551810281 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.44768453771218475 1101 2 0.445808076064548 1101 3 0.2698669008682799 1101 4 0.19542374759191006 1101 5 0.14759556214933411 1101 6 0.1570529570488901 1101 7 0.1391386148488607 1101 8 0.26805966187770736 1101 9 0.2949143112691175 1101 10-11 0.21186392989861247 1101 12-13 0.3651786544909399 1101 14-15 0.531564430587899 1101 16-17 0.4695733905687831 1101 18-19 0.4664855762981759 1101 20-21 0.2747258032677493 1101 22-23 0.25282787606789725 1101 24-25 0.22834387823652946 1101 26-27 0.3093998652338428 1101 28-29 0.35658531015332784 1101 30-31 0.16143930025128128 1101 32-33 0.3055720812610616 1101 34-35 0.38898050562682585 1101 36-37 0.4512491839789874 1101 38-39 0.3890847746843846 1101 40-41 0.4803461662249049 1101 42-43 0.6188414801656137 1101 44-45 0.5979405041302783 1101 46-47 0.5470318394585476 1101 48-49 0.5237889110158136 1101 50-51 0.46355244666664674 1101 52-53 0.43805720502994205 1101 54-55 0.316127880690793 1101 56-57 0.4799573607383323 1101 58-59 0.4813508432903646 1101 60-61 0.5443255919293435 1101 62-63 0.42540454568811015 1101 64-65 0.40973733851814487 1101 66-67 0.1429557168253659 1101 68-69 0.14211871597226633 1101 70-71 0.16850269744518798 1101 72-73 0.22860931991095512 1101 74-75 0.08980736639362519 1101 76-77 0.5085680801484891 1101 78-79 0.45898149061305205 1101 80-81 0.33548708165173124 1101 82-83 0.4010548072202056 1101 84-85 0.34151486208533655 1101 86-87 0.2737846064538658 1101 88-89 0.32952681671274675 1101 90-91 0.2624282794606039 1101 92-93 0.42217435276573667 1101 94-95 0.2858540347921057 1101 96-97 0.28536074470611084 1101 98-99 0.44903555132630757 1101 100-101 0.3711947789084107 1102 1 0.3662736927430572 1102 2 0.4380718350431252 1102 3 0.16798348059032264 1102 4 0.06580509494692421 1102 5 0.199610694728662 1102 6 0.19042145511414077 1102 7 0.1281394459524492 1102 8 -0.014099314490067627 1102 9 -0.03766667642460675 1102 10-11 0.19976508591326336 1102 12-13 0.28139367197312737 1102 14-15 0.3401434161717489 1102 16-17 0.3525401194818656 1102 18-19 0.1773763839332716 1102 20-21 0.14462821250094038 1102 22-23 0.15544833945477876 1102 24-25 0.1460777496109884 1102 26-27 -0.12845389784152417 1102 28-29 0.10427006834915886 1102 30-31 0.24755765962655119 1102 32-33 0.24345676028443108 1102 34-35 0.41088385028334073 1102 36-37 0.4163062178580219 1102 38-39 0.2969970466231402 1102 40-41 0.3046630964888877 1102 42-43 0.26429168890871324 1102 44-45 0.12019100552132045 1102 46-47 0.3004258578616543 1102 48-49 0.26757256017541664 1102 50-51 0.17884799967523435 1102 52-53 0.282867781807532 1102 54-55 0.44620250039009335 1102 56-57 0.45664241807619277 1102 58-59 0.46532411947582375 1102 60-61 0.4070644823956968 1102 62-63 0.3980347778236961 1102 64-65 0.2859398652664886 1102 66-67 0.28697619250954176 1102 68-69 0.4171241910312169 1102 70-71 0.30611562701279027 1102 72-73 0.444224568001939 1102 74-75 0.10309984231545499 1102 76-77 0.3330109194967861 1102 78-79 0.48170937569277683 1102 80-81 0.6103637114748786 1102 82-83 0.6385081137758348 1102 84-85 0.7847267561932085 1102 86-87 0.6657414857877129 1102 88-89 0.5399452493881718 1102 90-91 0.669816880146076 1102 92-93 0.7021070354479022 1102 94-95 0.25580114261380515 1102 96-97 0.3561931540152976 1102 98-99 0.0514780493901732 1102 100-101 0.04336120273807964 1103 1 0.3072560335319672 1103 2 0.3592121930096752 1103 3 0.1572295894390301 1103 4 0.10892919700820158 1103 5 -0.019801613352072422 1103 6 -0.0949852998563756 1103 7 0.06599724385102945 1103 8 0.018947168261824743 1103 9 -0.21967163374768006 1103 10-11 0.0168606035501071 1103 12-13 0.12961522731153252 1103 14-15 0.13654334563909742 1103 16-17 0.05372882170117066 1103 18-19 0.08451824873367997 1103 20-21 0.12309989157204626 1103 22-23 -0.027222369926001022 1103 24-25 -0.0065442815556266964 1103 26-27 0.0322523370174963 1103 28-29 0.07152147916911389 1103 30-31 0.07695614838441145 1103 32-33 0.12728912101083978 1103 34-35 0.10745687194607711 1103 36-37 -0.005768564115271602 1103 38-39 0.1745083528864555 1103 40-41 0.10183430981742703 1103 42-43 0.03832622318951451 1103 44-45 0.1466861674224731 1103 46-47 0.3166690726008383 1103 48-49 0.4812936649998889 1103 50-51 0.3284056010041354 1103 52-53 0.3425176299552817 1103 54-55 0.46027162639305885 1103 56-57 0.4609697229609111 1103 58-59 0.4677217953716237 1103 60-61 0.27132269734527625 1103 62-63 0.19248220194600663 1103 64-65 0.22472407209813738 1103 66-67 0.2101993007684726 1103 68-69 0.2650000963170882 1103 70-71 0.10556913257224565 1103 72-73 0.047573098805539615 1103 74-75 -0.0798679257336552 1103 76-77 0.008556896297157834 1103 78-79 0.04306676849617119 1103 80-81 0.0706911005178732 1103 82-83 0.020384128601051543 1103 84-85 0.20119616299092513 1103 86-87 0.3289584424775924 1103 88-89 -0.04756265714805252 1103 90-91 -0.268119732643715 1103 92-93 -0.07985958310606023 1103 94-95 -0.04081152026752122 1103 96-97 -0.5372587979557046 1103 98-99 -0.6475754705964114 1103 100-101 -0.1253041672677817 1104 1 0.3114464504425527 1104 2 0.28358392285041845 1104 3 0.0991777450903264 1104 4 0.024705358119803122 1104 5 0.08610265451234511 1104 6 0.07703327974855512 1104 7 0.012413574879346356 1104 8 0.08453422621165885 1104 9 0.0824239970304248 1104 10-11 0.05896196492393102 1104 12-13 0.027497733206182318 1104 14-15 0.1317010897075761 1104 16-17 0.09736142814114146 1104 18-19 0.11863267352675422 1104 20-21 0.021897863382982052 1104 22-23 0.20345619888045974 1104 24-25 0.13750840613924709 1104 26-27 0.13671794597126308 1104 28-29 0.07288070890427178 1104 30-31 -0.017180419118695056 1104 32-33 0.06570844468359383 1104 34-35 0.19664430335922845 1104 36-37 0.20655829165239226 1104 38-39 0.0105558141740687 1104 40-41 0.3144933387218529 1104 42-43 0.2029622751339133 1104 44-45 0.13394566270122255 1104 46-47 0.06993005445590228 1104 48-49 0.20441769081485717 1104 50-51 0.1560657311965059 1104 52-53 -0.0837954853771734 1104 54-55 -0.045683775018275696 1104 56-57 0.20132598149264425 1104 58-59 0.1319230172489867 1104 60-61 -0.04034829109152582 1104 62-63 -0.19006787416956428 1104 64-65 -0.15537701898909972 1104 66-67 -0.07016278581809843 1104 68-69 -0.12201896733475337 1104 70-71 0.06111120989370633 1104 72-73 -0.026970451447954957 1104 74-75 -0.21362779428080358 1104 76-77 0.05927039444900828 1104 78-79 0.15868088759250654 1104 80-81 0.2275895487739028 1104 82-83 0.049956796388279656 1104 84-85 0.21884876933992814 1104 86-87 0.12587152259482792 1104 88-89 0.08753987308538669 1104 90-91 0.08217348933500546 1104 92-93 0.3017597584991698 1104 94-95 0.38829948371075673 1104 96-97 0.13968975078067203 1104 98-99 0.020472787257002523 1104 100-101 0.20670686250467796 1105 1 0.39075445249211427 1105 2 0.38879284638325373 1105 3 0.2241732545846773 1105 4 0.16442689004713884 1105 5 0.014608132298995713 1105 6 0.0028205047799190197 1105 7 0.09970522329512477 1105 8 0.07848732705549821 1105 9 0.07974702541483225 1105 10-11 0.028497114081197594 1105 12-13 -0.022051451538580125 1105 14-15 0.08167638404840005 1105 16-17 0.11279064800322658 1105 18-19 0.0035602361559128326 1105 20-21 0.1249073845154598 1105 22-23 0.10428251494524687 1105 24-25 -0.0982960813412177 1105 26-27 0.02206147122933544 1105 28-29 0.13422101612419368 1105 30-31 -0.16103778553323878 1105 32-33 -0.04307098230897566 1105 34-35 -0.1383322924404311 1105 36-37 -0.05055939615000682 1105 38-39 -0.034921821217039906 1105 40-41 -0.22773884279416023 1105 42-43 -0.06421713900162729 1105 44-45 -0.268869395902243 1105 46-47 0.01671630197509444 1105 48-49 0.12733493126835782 1105 50-51 -0.11162008592740591 1105 52-53 -0.06713775770538888 1105 54-55 -0.048987841809271515 1105 56-57 0.021062904037250973 1105 58-59 0.08563472741631273 1105 60-61 0.09753512718852164 1105 62-63 -0.002016211611049812 1105 64-65 -0.002681392079846745 1105 66-67 0.007649168821806995 1105 68-69 -0.16667082237052 1105 70-71 -0.1804972167528618 1105 72-73 -0.28587756636267514 1105 74-75 -0.3065562090031122 1105 76-77 -0.2715175837268191 1105 78-79 -0.21519892819907582 1105 80-81 -0.3181827268126831 1105 82-83 -0.09050685068381625 1105 84-85 0.20684525382547037 1105 86-87 0.03233381715813266 1105 88-89 -0.23002663839360693 1105 90-91 -0.3769592853058974 1105 92-93 0.0016994439179782717 1105 94-95 0.04110792472694058 1105 96-97 -0.0930500059820325 1105 98-99 -0.10250955765038228 1105 100-101 -0.050227896277963424 1106 1 0.26483030679454345 1106 2 0.3294744098780136 1106 3 0.1389574065007686 1106 4 -0.003912116996730219 1106 5 -0.16266485865958913 1106 6 -0.09196345683589868 1106 7 -0.030619462732445868 1106 8 -0.04558231179448313 1106 9 0.016633326220016897 1106 10-11 -0.03851728029278689 1106 12-13 -0.0744099625187502 1106 14-15 -0.07714699550641768 1106 16-17 0.0984533640976295 1106 18-19 -0.04178371470487008 1106 20-21 -0.06951902744350491 1106 22-23 -0.06721685783119113 1106 24-25 0.07460153803306468 1106 26-27 0.04035040167734394 1106 28-29 0.025160236979623107 1106 30-31 -0.019648059476622848 1106 32-33 -0.03214774667642217 1106 34-35 -0.13290827427473317 1106 36-37 2.3604364159268698E-4 1106 38-39 0.12439480436625416 1106 40-41 -0.026441977131725025 1106 42-43 -0.1063945854113797 1106 44-45 0.10527014760424436 1106 46-47 0.03891866544308442 1106 48-49 0.009724145048927824 1106 50-51 -0.04917305155764495 1106 52-53 0.08964679997999525 1106 54-55 0.046282613244720494 1106 56-57 0.0030548199589119918 1106 58-59 -0.0222727399970708 1106 60-61 0.26236063872025284 1106 62-63 -0.09691527419805368 1106 64-65 0.06031413678456232 1106 66-67 0.17174800955552172 1106 68-69 0.24677600972847102 1106 70-71 0.39971833820723646 1106 72-73 0.3151266052034565 1106 74-75 0.23887307004430625 1106 76-77 0.23043437527636357 1106 78-79 0.2681505653106129 1106 80-81 0.24420184609672546 1106 82-83 0.4420585903570142 1106 84-85 0.44405377596276097 1106 86-87 0.1930259760809605 1106 88-89 0.1853336322502166 1106 90-91 0.2815369005548547 1106 92-93 0.3895940138838654 1106 94-95 0.45088932803184534 1106 96-97 0.2856661056063672 1106 98-99 0.21117757458283037 1106 100-101 0.1669093185056667 1107 1 0.6508268179494578 1107 2 0.618100070881173 1107 3 0.412286776422647 1107 4 0.2025425071571334 1107 5 0.039602108391946444 1107 6 0.18477359949669392 1107 7 0.2078657839899165 1107 8 0.2184053364423164 1107 9 0.3482561346099331 1107 10-11 0.3179686013576628 1107 12-13 0.4494061964662066 1107 14-15 0.6556524180651451 1107 16-17 0.42354355126451537 1107 18-19 0.39962868738322044 1107 20-21 0.34714535765406396 1107 22-23 0.47699392134421004 1107 24-25 0.2717630053297029 1107 26-27 0.3371859631258687 1107 28-29 0.4522155396069465 1107 30-31 0.46195222656695023 1107 32-33 0.5192216184997989 1107 34-35 0.482926173768071 1107 36-37 0.718283483329941 1107 38-39 0.7774015538995087 1107 40-41 0.7846186787085685 1107 42-43 0.5876766595170153 1107 44-45 0.5601447851606949 1107 46-47 0.8202446091000652 1107 48-49 0.8138458973982878 1107 50-51 0.5929419760297847 1107 52-53 0.47480336776997234 1107 54-55 0.47547069406702036 1107 56-57 0.5409660238972904 1107 58-59 0.2611199793203909 1107 60-61 0.577725148717434 1107 62-63 0.27419269486647835 1107 64-65 0.44737125162962244 1107 66-67 0.6189216995437334 1107 68-69 0.5825436143155969 1107 70-71 0.317620148599719 1107 72-73 0.4717331818589656 1107 74-75 0.6899013080471654 1107 76-77 0.5879115986980388 1107 78-79 0.6479482162070731 1107 80-81 0.6178330676097055 1107 82-83 0.5036011461837511 1107 84-85 0.6103628881585976 1107 86-87 0.7818789139597015 1107 88-89 0.9156022967891566 1107 90-91 0.7286157424564585 1107 92-93 0.9334038887756577 1107 94-95 0.9538121810101394 1107 96-97 1.0173028967554423 1107 98-99 1.1271630299057982 1107 100-101 1.2235686645949286 1108 1 0.37249738482670125 1108 2 0.3563902570418982 1108 3 0.12631913942556494 1108 4 0.1135075206941849 1108 5 0.02427585490273998 1108 6 0.010425660486895083 1108 7 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-1.090642141731216 2316 64-65 -0.9228979547020444 2316 66-67 -1.0814192111167813 2316 68-69 -1.0395674073977048 2316 70-71 -0.9529920402728997 2316 72-73 -0.8371401640898171 2316 74-75 -1.1482455502914632 2316 76-77 -1.169168189417654 2316 78-79 -1.0614623510737893 2316 80-81 -1.0706402845547167 2316 82-83 -0.6313866124559908 2316 84-85 -0.6533710892993447 2316 86-87 -0.6958479396826647 2316 88-89 -0.8188560100701494 2316 90-91 -1.0516505459769228 2316 92-93 -1.0704139825743795 2316 94-95 -1.4735912866270873 2316 96-97 -1.5937719084877955 2316 98-99 -1.6902300610635699 2316 100-101 -2.370829229683917 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 16.0 4 44.0 5 90.0 6 217.0 7 536.0 8 1064.0 9 2032.0 10 3315.0 11 5128.0 12 7241.0 13 9584.0 14 12073.0 15 15970.0 16 20332.0 17 25127.0 18 21233.0 19 13475.0 20 11702.0 21 11952.0 22 12788.0 23 14395.0 24 17000.0 25 19944.0 26 24560.0 27 30060.0 28 37190.0 29 44893.0 30 54450.0 31 65791.0 32 81114.0 33 100930.0 34 128981.0 35 168413.0 36 227345.0 37 284098.0 38 171456.0 39 10283.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.52652982491695 19.916261444004803 15.532775727416901 21.02443300366135 2 15.761463088278576 21.661004800448676 39.4563883196133 23.121143791659442 3 19.25709893180626 26.83350294544936 30.326767209183707 23.58263091356067 4 12.86289299053559 17.908099744956544 37.16484751785909 32.06415974664878 5 12.896582480574997 38.69252719016985 33.293832705069896 15.117057624185263 6 29.773341728979364 37.3214464844038 17.638531180921742 15.266680605695102 7 27.455817107055363 34.57336300009789 20.41525816007242 17.555561732774322 8 27.17989685924683 33.584618564126984 19.521266888482536 19.71421768814365 9 27.400474752773217 16.470706255182034 20.563212944990077 35.56560604705467 10-11 24.864184788454587 27.36188544749828 26.3415340139302 21.43239575011693 12-13 26.724384858311044 25.107081003274068 27.403128554007623 20.765405584407265 14-15 23.38784361251383 25.683100725726067 27.15506861599607 23.773987045764038 16-17 21.123640793654438 29.205850041031507 27.612540532962377 22.05796863235168 18-19 21.068052190397115 26.573617580826497 31.68406578216253 20.674264446613858 20-21 22.55959918432487 25.268404432974954 31.66741750112021 20.50457888157996 22-23 21.924896332258886 25.5091231237982 31.646549504896647 20.91943103904627 24-25 21.558610995959697 25.74874941534639 31.968315702073813 20.724323886620105 26-27 21.297079359727054 25.869313713137732 31.924302592629854 20.90930433450536 28-29 20.97942623574926 26.06104811019406 32.04950248545769 20.910023168598997 30-31 21.165893674557733 25.770851902769177 31.93633767921079 21.1269167434623 32-33 21.020973383515713 25.914571503851906 31.689080739005682 21.375374373626695 34-35 21.61237670849604 25.87704508894634 31.511078559696593 20.99949964286103 36-37 21.914937333059378 25.473778991769297 31.388734454126833 21.22254922104449 38-39 21.5183910796556 25.822836748802292 31.395574393409813 21.263197778132298 40-41 21.562401232318507 25.89923536062781 31.35731495733236 21.18104844972132 42-43 21.55653031043821 26.057301163198986 30.785429764767525 21.600738761595277 44-45 21.580096821894728 26.163364436959107 30.585101079229943 21.67143766191622 46-47 22.06585877374194 26.53882028537009 29.800395637293605 21.594925303594366 48-49 22.221624584583086 26.793612959255146 29.129284935965817 21.855477520195958 50-51 22.19165491310827 27.032368514241444 28.94529791191539 21.830678660734897 52-53 22.450552638099094 27.120178391215806 28.736184047522645 21.693084923162456 54-55 22.01825741688675 27.04007445593381 28.854061534928416 22.08760659225102 56-57 22.22887305111996 27.317905357529725 28.37279866712151 22.080422924228795 58-59 22.45967200989119 26.839913560864538 28.55313553371211 22.147278895532168 60-61 22.17294940420884 27.029837148904733 28.980091948396492 21.817121498489936 62-63 22.285000332372476 27.06028632377914 28.935531826175236 21.71918151767315 64-65 22.0248733336033 27.120872651844042 28.800097670474567 22.054156344078095 66-67 22.063999303829902 27.24584352610154 28.509313692312155 22.180843477756397 68-69 22.253601897968483 27.10663140809102 28.634076655978706 22.005690037961788 70-71 22.230595145651602 27.105822136142933 28.57324579028949 22.090336927915978 72-73 22.282070743389333 27.153368390392707 28.374282250822745 22.190278615395215 74-75 22.06612658974418 27.7144545710546 28.126582492660862 22.092836346540363 76-77 22.09735032544581 27.442293874753975 28.333485415519753 22.126870384280465 78-79 22.13711555063879 27.458374265821494 28.254461427167165 22.150048756372552 80-81 22.054639491469853 27.78690568107472 28.071510918983606 22.086943908471827 82-83 22.228302780792873 27.695970594136714 27.981471453540234 22.094255171530172 84-85 21.972552703710072 27.669421347756103 27.91526673892555 22.442759209608276 86-87 22.05988292473025 27.52637698817039 27.8418492719359 22.57189081516346 88-89 22.178757594010687 27.470393898738866 27.71904192984964 22.631806577400802 90-91 22.008465770928545 27.463889310317107 27.92882369545468 22.598821223299666 92-93 22.114336372602104 27.451830571240716 28.002748394956683 22.431084661200494 94-95 21.81495957708154 27.2703840743824 28.091261669464267 22.82339467907179 96-97 21.931195491034707 27.139338075095836 28.072158674385477 22.857307759483977 98-99 21.721532038232365 27.381295924901107 27.741004785542025 23.156167251324508 100-101 21.604995163602002 27.41716403049484 27.946584167376535 23.031256638526617 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1117.0 1 870.5 2 1142.5 3 1392.5 4 848.5 5 647.0 6 1116.5 7 2153.0 8 3378.5 9 4058.5 10 3506.0 11 2637.0 12 2494.0 13 2743.5 14 2999.0 15 3232.0 16 3477.0 17 3651.0 18 3968.0 19 4319.5 20 4671.5 21 5206.5 22 5878.5 23 6774.5 24 7811.5 25 9242.5 26 11311.5 27 13656.5 28 15726.0 29 18382.5 30 22015.5 31 26019.5 32 30394.5 33 33498.0 34 36210.0 35 38604.5 36 41773.0 37 45266.5 38 46270.0 39 46297.0 40 46917.5 41 49077.0 42 50375.0 43 51459.0 44 53705.0 45 56083.0 46 56386.5 47 57449.0 48 63755.0 49 62899.0 50 56083.0 51 52116.0 52 49484.0 53 49377.0 54 48568.0 55 45342.0 56 41535.0 57 38033.5 58 36105.5 59 33165.5 60 28255.0 61 24196.5 62 20853.5 63 16288.5 64 11837.0 65 9344.5 66 7224.5 67 5545.5 68 4078.0 69 2947.0 70 2345.0 71 1906.5 72 1649.0 73 1512.5 74 1264.5 75 1044.0 76 779.0 77 527.5 78 356.0 79 236.5 80 165.5 81 125.0 82 89.0 83 59.5 84 34.0 85 15.0 86 10.5 87 6.0 88 4.0 89 3.0 90 3.5 91 2.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0050603507559102 2 0.01142114034949898 3 0.004230051981295919 4 0.012448438687813704 5 0.0 6 0.0 7 0.0 8 0.0 9 0.004230051981295919 10-11 2.4171725607405247E-4 12-13 2.4171725607405247E-4 14-15 3.021465700925656E-5 16-17 0.0 18-19 3.021465700925656E-5 20-21 3.021465700925656E-5 22-23 0.0012085862803702624 24-25 0.0 26-27 3.021465700925656E-5 28-29 0.0 30-31 6.042931401851312E-5 32-33 0.0037466174691478137 34-35 4.8343451214810494E-4 36-37 0.0027797484448516038 38-39 1.2085862803702623E-4 40-41 0.0035653295270922742 42-43 0.009970836813054666 44-45 0.0021150259906479595 46-47 0.001661806135509111 48-49 0.009849978185017639 50-51 4.8343451214810494E-4 52-53 0.0021754553046664724 54-55 0.009426972986888047 56-57 0.003716402812138557 58-59 0.0012085862803702624 60-61 0.0056501408607309775 62-63 0.0033840415850367347 64-65 0.017101495867239212 66-67 0.0035049002130737614 68-69 2.4171725607405247E-4 70-71 0.0012690155943887757 72-73 0.0 74-75 0.0 76-77 0.0011481569663517493 78-79 0.010303198040156487 80-81 0.015318831103693076 82-83 0.011481569663517493 84-85 0.007704737537360424 86-87 0.009910407499036152 88-89 0.028975856071877044 90-91 0.024111296293386736 92-93 0.0025380311887775514 94-95 0.008067313421471501 96-97 0.010091695441091692 98-99 0.009336329015860277 100-101 0.011209637750434185 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1654826.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.79932994536306 #Duplication Level Percentage of deduplicated Percentage of total 1 76.20206294615419 40.23417864011319 2 13.508279630459194 14.26456226405684 3 4.403539326860375 6.975117775388487 4 1.9369135585071477 4.0907095220506475 5 1.0093937940863809 2.6647657989384346 6 0.6287525603106698 1.9918628331500907 7 0.4145698030627455 1.5322305472105862 8 0.2936798824814052 1.240488081076093 9 0.2003305625746996 0.951958752436959 >10 1.2266185372594527 12.216043338753023 >50 0.1121978040805063 4.136022764972106 >100 0.05802095179222471 5.516199927191518 >500 0.0042592605658595215 1.4843090660326903 >1k 0.0010360363538577215 0.9440961763517374 >5k 1.1511515042863572E-4 0.39197040116476883 >10k+ 2.3023030085727144E-4 1.3654841111129938 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12082 0.7301069719716756 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10384 0.6274979967682403 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6449 0.38970864610539113 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2818 0.17028980690416998 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2460 0.14865611248554228 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1918 0.11590342428750817 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1876 0.11336539309873062 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 6.0429314018513124E-5 6 0.0 0.0 0.0 0.0 6.0429314018513124E-5 7 0.0 0.0 0.0 0.0 6.0429314018513124E-5 8 0.0 0.0 0.0 0.0 6.0429314018513124E-5 9 0.0 0.0 0.0 0.0 6.0429314018513124E-5 10-11 0.0 0.0 0.0 0.0 6.0429314018513124E-5 12-13 0.0 0.0 0.0 0.0 6.0429314018513124E-5 14-15 0.0 0.0 0.0 0.0 6.0429314018513124E-5 16-17 0.0 0.0 0.0 0.0 6.0429314018513124E-5 18-19 0.0 0.0 0.0 0.0 6.0429314018513124E-5 20-21 0.0 0.0 0.0 0.0 6.0429314018513124E-5 22-23 0.0 0.0 0.0 3.0214657009256562E-5 6.0429314018513124E-5 24-25 0.0 0.0 0.0 1.2085862803702625E-4 6.0429314018513124E-5 26-27 0.0 0.0 0.0 3.9279054112033535E-4 6.0429314018513124E-5 28-29 0.0 0.0 0.0 9.064397102776969E-4 6.0429314018513124E-5 30-31 0.0 0.0 0.0 0.002115025990647959 6.0429314018513124E-5 32-33 0.0 0.0 0.0 0.0035351148700830176 6.0429314018513124E-5 34-35 0.0 0.0 0.0 0.005710570174749491 6.0429314018513124E-5 36-37 0.0 0.0 0.0 0.008097528078480758 6.0429314018513124E-5 38-39 0.0 0.0 0.0 0.012236936088748908 6.0429314018513124E-5 40-41 0.0 0.0 0.0 0.01670870532611888 6.0429314018513124E-5 42-43 0.0 0.0 0.0 0.02169412373264621 6.0429314018513124E-5 44-45 0.0 0.0 0.0 0.02480623340459964 9.064397102776969E-5 46-47 0.0 0.0 0.0 0.030003154410191767 1.510732850462828E-4 48-49 0.0 0.0 0.0 0.03438427967653397 1.8128794205553937E-4 50-51 0.0 0.0 0.0 0.039037336855959476 1.8128794205553937E-4 52-53 0.0 0.0 0.0 0.05106277034564359 1.8128794205553937E-4 54-55 0.0 6.0429314018513124E-5 0.0 0.06831533949792909 1.8128794205553937E-4 56-57 0.0 6.0429314018513124E-5 0.0 0.09206405990720475 1.8128794205553937E-4 58-59 0.0 6.0429314018513124E-5 0.0 0.12545125590243325 1.8128794205553937E-4 60-61 0.0 6.0429314018513124E-5 0.0 0.16593889629483705 1.8128794205553937E-4 62-63 0.0 6.0429314018513124E-5 0.0 0.19279972637606613 2.417172560740525E-4 64-65 0.0 6.0429314018513124E-5 0.0 0.22588477580120206 2.417172560740525E-4 66-67 0.0 6.0429314018513124E-5 0.0 0.2848637862832709 2.417172560740525E-4 68-69 0.0 6.0429314018513124E-5 0.0 0.36728937060452277 2.417172560740525E-4 70-71 0.0 6.0429314018513124E-5 0.0 0.4514976196893208 2.417172560740525E-4 72-73 0.0 6.0429314018513124E-5 0.0 0.5448911245049328 2.7193191308330904E-4 74-75 0.0 6.0429314018513124E-5 0.0 0.6296734520729068 3.0214657009256565E-4 76-77 0.0 6.0429314018513124E-5 0.0 0.7103465862876218 3.0214657009256565E-4 78-79 0.0 6.0429314018513124E-5 0.0 0.7858832288107631 3.0214657009256565E-4 80-81 0.0 6.0429314018513124E-5 0.0 0.8632931800684784 3.0214657009256565E-4 82-83 0.0 6.0429314018513124E-5 0.0 0.9698602753401264 3.0214657009256565E-4 84-85 0.0 6.0429314018513124E-5 0.0 1.1442592755975554 3.0214657009256565E-4 86-87 0.0 6.0429314018513124E-5 0.0 1.370567056596887 3.0214657009256565E-4 88-89 0.0 6.0429314018513124E-5 0.0 1.6388127815250666 3.0214657009256565E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11265 0.0 33.139683 1 TTACGCT 65 0.00584566 29.229895 4 GTATCAA 16295 0.0 29.035042 1 GAGTACT 11665 0.0 23.510183 12-13 ATCAACG 20195 0.0 23.33322 3 GTACATG 11945 0.0 23.138557 1 TCAACGC 20470 0.0 23.064772 4 TATCAAC 20455 0.0 22.992971 2 CAACGCA 20715 0.0 22.787848 5 TAGCCGC 105 0.0021993245 22.614271 5 AACGCAG 21115 0.0 22.35616 6 TACATGG 12295 0.0 22.294958 2 GTACTTT 12405 0.0 22.050299 14-15 ACATGGG 12250 0.0 21.443514 3 AGTACTT 11870 0.0 20.943764 12-13 ACGCAGA 23525 0.0 19.985151 7 CGCAGAG 23650 0.0 19.899601 8 ACTTTTT 14100 0.0 19.569153 16-17 GCAGAGT 24465 0.0 19.023163 9 CATGGGA 7530 0.0 18.860659 4 >>END_MODULE