FastQCFastQC Report
Thu 26 May 2016
SRR936757_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936757_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1672317
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110010.6578298253261792No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84820.5072004889025227No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51920.31046745324002567No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22260.13310873476739157No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22140.13239116746406335No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA149600.032.9722561
GGTATCA97850.030.741551
TCAACGC181600.027.1686694
ATCAACG181050.027.115883
TATCAAC183000.026.8269372
CAACGCA183500.026.755445
GTACATG123850.026.2447831
AACGCAG187800.026.1910936
TACATGG126500.025.4343152
TACCTGG12150.025.4248812
ACATGGG128100.024.3746343
GAGTACT95050.024.17824212-13
ACGCAGA208250.023.554217
CGCAGAG208950.023.4966438
GTACCTG13550.023.1466161
GCAGAGT214150.022.3921059
AGTACTT97950.022.34629412-13
GTACTTT103150.022.04888214-15
GTACACG3251.8189894E-1221.9326341
GTATAGG5900.021.7467631
Produced by FastQC (version 0.11.5)