##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936757_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1672317 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.170114278572782 31.0 31.0 34.0 28.0 34.0 2 31.179003741515515 31.0 31.0 34.0 28.0 34.0 3 31.2050454548988 31.0 31.0 34.0 27.0 34.0 4 34.85765856592978 37.0 35.0 37.0 32.0 37.0 5 34.842657223480956 37.0 35.0 37.0 32.0 37.0 6 34.98241601323194 37.0 35.0 37.0 32.0 37.0 7 34.83838470816239 37.0 35.0 37.0 32.0 37.0 8 34.78750858838366 37.0 35.0 37.0 32.0 37.0 9 36.208407257714896 39.0 35.0 39.0 32.0 39.0 10-11 36.1069949656674 39.0 35.0 39.0 30.5 39.0 12-13 35.97930924579491 39.0 35.0 39.0 30.0 39.0 14-15 36.93688726479489 39.0 36.0 41.0 31.0 41.0 16-17 36.813186734333264 39.0 36.0 41.0 30.0 41.0 18-19 36.8086406464803 39.0 36.0 41.0 30.0 41.0 20-21 36.74879314149172 39.0 36.0 41.0 30.0 41.0 22-23 36.60356439598473 39.0 36.0 41.0 30.0 41.0 24-25 36.709224686467934 39.0 36.0 41.0 30.0 41.0 26-27 36.6691428718359 39.0 36.0 41.0 30.0 41.0 28-29 36.55145107058052 39.0 36.0 41.0 30.0 41.0 30-31 36.303195207607175 39.0 35.0 40.5 30.0 41.0 32-33 36.05829845657253 38.5 35.0 40.0 29.5 41.0 34-35 35.80093487060169 38.0 35.0 40.0 28.5 41.0 36-37 35.56838805083008 38.0 35.0 40.0 27.0 41.0 38-39 35.32958434315982 38.0 34.5 40.0 26.5 41.0 40-41 35.0991420884916 38.0 34.0 40.0 25.5 41.0 42-43 34.802246523834896 38.0 34.0 40.0 24.5 41.0 44-45 34.44253003467644 38.0 33.0 40.0 24.0 41.0 46-47 34.00832587362324 38.0 33.0 40.0 22.5 41.0 48-49 33.77212843019595 38.0 33.0 40.0 19.5 41.0 50-51 32.82187348451281 37.0 32.0 39.5 16.5 40.5 52-53 32.942607771134305 37.5 32.5 39.5 17.5 40.5 54-55 33.33308128781804 38.0 33.0 40.0 17.0 41.0 56-57 33.235652690249516 38.0 33.0 40.0 14.5 41.0 58-59 32.97897976280812 37.0 32.5 40.0 13.5 41.0 60-61 32.66649385254111 37.0 32.0 40.0 11.0 41.0 62-63 32.33328130970384 36.0 31.0 39.5 8.0 41.0 64-65 31.98102423164986 36.0 31.0 39.0 7.5 41.0 66-67 31.59847385394037 35.0 31.0 39.0 6.5 40.5 68-69 31.18608224397647 35.0 30.5 38.5 2.0 40.0 70-71 30.763138507830753 35.0 30.0 38.0 2.0 40.0 72-73 30.372537622950674 35.0 29.5 37.0 2.0 39.5 74-75 29.950433739536223 34.5 29.0 37.0 2.0 39.0 76-77 29.529486933398395 34.0 29.0 36.0 2.0 39.0 78-79 29.090011044556743 34.0 29.0 36.0 2.0 38.0 80-81 28.628511520244068 34.0 27.5 35.0 2.0 37.0 82-83 28.15310494362014 34.0 27.0 35.0 2.0 37.0 84-85 27.825447567656134 34.0 26.0 35.0 2.0 36.0 86-87 27.49818036891331 33.5 26.0 35.0 2.0 36.0 88-89 27.0813180156633 33.0 25.5 35.0 2.0 35.5 90-91 26.66063491550944 33.0 25.0 35.0 2.0 35.0 92-93 26.28917962324129 33.0 24.5 35.0 2.0 35.0 94-95 25.819890905850983 33.0 23.0 35.0 2.0 35.0 96-97 25.223200804632135 32.0 19.0 35.0 2.0 35.0 98-99 24.51043940831792 32.0 7.0 35.0 2.0 35.0 100-101 22.081722245244173 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5545159358245044 1101 2 0.6179905087867574 1101 3 0.5769907330329787 1101 4 0.5915325265342304 1101 5 0.5311937266119742 1101 6 0.3486923814433851 1101 7 0.5002891072668234 1101 8 0.5523965845118681 1101 9 0.8053254965136176 1101 10-11 0.6182540564236376 1101 12-13 0.8391625762707804 1101 14-15 0.997023580101164 1101 16-17 0.8795003467396683 1101 18-19 0.9135856945072689 1101 20-21 1.0141128802759454 1101 22-23 0.8775918850567095 1101 24-25 0.8899900533156497 1101 26-27 0.8167721016197333 1101 28-29 0.9339446048099305 1101 30-31 0.9812533999383461 1101 32-33 1.051422351721591 1101 34-35 0.8625479584455817 1101 36-37 0.7911726536328985 1101 38-39 0.7804022931456558 1101 40-41 0.7416029008940015 1101 42-43 0.7385487789206735 1101 44-45 0.8417620667691139 1101 46-47 0.8723361604080182 1101 48-49 0.9073922618880559 1101 50-51 0.7765042513974052 1101 52-53 0.928484776726286 1101 54-55 1.086079934711286 1101 56-57 0.9002493571145322 1101 58-59 1.0055391380546226 1101 60-61 0.9879890974424015 1101 62-63 0.9579034806645836 1101 64-65 0.9982424843701772 1101 66-67 1.057460115453864 1101 68-69 0.9690962555245299 1101 70-71 0.9286443136671352 1101 72-73 0.9205037822120126 1101 74-75 0.9429612557144473 1101 76-77 0.8260799805007757 1101 78-79 0.8271305161056226 1101 80-81 0.7339889099804857 1101 82-83 0.9457570044871382 1101 84-85 0.9932696609301779 1101 86-87 0.8944821979782098 1101 88-89 1.0843453077311374 1101 90-91 1.0867702074701278 1101 92-93 1.096889071811713 1101 94-95 1.088386178183267 1101 96-97 1.1847210396540362 1101 98-99 1.0576049094766589 1101 100-101 1.376206105046517 1102 1 0.28187825587081505 1102 2 0.37358800876075904 1102 3 0.2854669720398988 1102 4 0.2557282856415455 1102 5 0.32111493350195275 1102 6 0.2591663533718247 1102 7 0.36975565052676984 1102 8 0.3443006184556481 1102 9 0.5124945255869946 1102 10-11 0.5105286571717045 1102 12-13 0.43118620380434436 1102 14-15 0.34747387596531354 1102 16-17 0.5363601657200405 1102 18-19 0.46636927864049227 1102 20-21 0.5344604296473037 1102 22-23 0.5477645504685071 1102 24-25 0.1798453970692222 1102 26-27 0.2702276630683045 1102 28-29 0.36615479834356535 1102 30-31 0.3334497622133199 1102 32-33 0.059696148246096925 1102 34-35 -0.08239583256721517 1102 36-37 0.1846664157005904 1102 38-39 0.04937260967002999 1102 40-41 0.00846455710922811 1102 42-43 0.09369771627095957 1102 44-45 -0.4766409601619799 1102 46-47 -0.2884749267843816 1102 48-49 -0.047547473342127944 1102 50-51 -0.1368607655710221 1102 52-53 -0.24996723157941148 1102 54-55 0.05472925510770921 1102 56-57 0.19255688943079718 1102 58-59 0.2708965148992277 1102 60-61 -0.016680945576631245 1102 62-63 0.015050670908522079 1102 64-65 -0.03885492720856121 1102 66-67 0.14282861970204408 1102 68-69 0.0867454961918952 1102 70-71 -0.13902537923732794 1102 72-73 -0.002710546833281313 1102 74-75 -0.1349245029422228 1102 76-77 0.01921218861442142 1102 78-79 -0.1003085864918134 1102 80-81 0.13482589178493143 1102 82-83 0.21561370016825165 1102 84-85 0.07090600628873034 1102 86-87 -0.06456653034328852 1102 88-89 -0.008135721952335473 1102 90-91 0.2906290939671443 1102 92-93 -0.07185003043906946 1102 94-95 0.20350679438490715 1102 96-97 0.1670438938157197 1102 98-99 0.2576288281946155 1102 100-101 0.4176678160321394 1103 1 0.35172948151696914 1103 2 0.5051669561291803 1103 3 0.4174883034340162 1103 4 0.35881588373868567 1103 5 0.38214271885480144 1103 6 0.3737862364127622 1103 7 0.34042801364527264 1103 8 0.29627771144719617 1103 9 0.5425523991136458 1103 10-11 0.5539247120758048 1103 12-13 0.5409853129762325 1103 14-15 0.4270378549975149 1103 16-17 0.5978682104432451 1103 18-19 0.6261835379502472 1103 20-21 0.7925218180059304 1103 22-23 0.703535237981761 1103 24-25 0.798731558933298 1103 26-27 0.6598286084369036 1103 28-29 0.2544740420486775 1103 30-31 0.424791458422753 1103 32-33 0.5271778923299664 1103 34-35 0.487884141980544 1103 36-37 0.48106068288841897 1103 38-39 0.7296516752095243 1103 40-41 0.5949400290360174 1103 42-43 0.5209255440441893 1103 44-45 0.49716596648105593 1103 46-47 0.5718468621439001 1103 48-49 0.5901324345450334 1103 50-51 0.6305452278235251 1103 52-53 0.6730306777015684 1103 54-55 0.7137617673035734 1103 56-57 0.5832183444500387 1103 58-59 0.5839741139053771 1103 60-61 0.5806829304346408 1103 62-63 0.38038564045675116 1103 64-65 0.6106845086003041 1103 66-67 0.5643312103756202 1103 68-69 0.6719074511656835 1103 70-71 0.7576158351977256 1103 72-73 0.7947290798673201 1103 74-75 0.5970316828590647 1103 76-77 0.4914025955471253 1103 78-79 0.7491338884347094 1103 80-81 0.5925797323448805 1103 82-83 0.46737717621588004 1103 84-85 0.5340548109428553 1103 86-87 0.6602160651783358 1103 88-89 0.7326863245282844 1103 90-91 0.6816723324606109 1103 92-93 0.7526250172838616 1103 94-95 0.6334116515191077 1103 96-97 0.5958397608581087 1103 98-99 0.7430331853742658 1103 100-101 1.011909248629177 1104 1 0.30553106799060004 1104 2 0.3116546518786194 1104 3 0.3388069775346203 1104 4 0.4383433804254864 1104 5 0.5031393457630315 1104 6 0.4285960798132038 1104 7 0.45461180657684963 1104 8 0.4136636232831563 1104 9 0.3674829474245911 1104 10-11 0.48112601185595594 1104 12-13 0.28007057917228906 1104 14-15 0.424298908344916 1104 16-17 0.33668494298759555 1104 18-19 0.49209831039213014 1104 20-21 0.7632429564340626 1104 22-23 0.4368035629886222 1104 24-25 0.5599496005308922 1104 26-27 0.2280115242873464 1104 28-29 0.6286003417322306 1104 30-31 0.4899668939688837 1104 32-33 0.12506468704275875 1104 34-35 0.17701780219081797 1104 36-37 0.23961657073022025 1104 38-39 0.4311625150823275 1104 40-41 0.4964340614068803 1104 42-43 0.5970200601620093 1104 44-45 0.6466232966769851 1104 46-47 0.6638524800997274 1104 48-49 0.473201967373754 1104 50-51 0.49706226908497086 1104 52-53 0.5552733587106289 1104 54-55 0.6577758817414079 1104 56-57 0.49563000847000893 1104 58-59 0.5838601319956567 1104 60-61 0.8198618936242852 1104 62-63 1.044833040703253 1104 64-65 0.6994001063484809 1104 66-67 0.8092715727221744 1104 68-69 0.7554059616732722 1104 70-71 0.9676183273445638 1104 72-73 0.912276305083985 1104 74-75 0.6226945166425324 1104 76-77 0.8422959046158347 1104 78-79 0.7371585433013514 1104 80-81 0.7699911258992991 1104 82-83 0.8744592200081165 1104 84-85 0.9568571817665763 1104 86-87 1.0722176841078301 1104 88-89 1.0307659225392065 1104 90-91 1.077808732100511 1104 92-93 1.178084229147256 1104 94-95 0.922582307552613 1104 96-97 1.012388098847314 1104 98-99 1.335949209685257 1104 100-101 1.5689002034884787 1105 1 0.21370778295080584 1105 2 0.21595401913160828 1105 3 0.3666334796343662 1105 4 0.2103326878416567 1105 5 0.37787287809234726 1105 6 0.31190551104353403 1105 7 0.2966418040298038 1105 8 0.32269008623615036 1105 9 0.532235877471102 1105 10-11 0.5158082142622291 1105 12-13 0.2680952177862963 1105 14-15 0.17003244569304599 1105 16-17 0.33001384094380626 1105 18-19 0.37085003090439983 1105 20-21 0.47187224297197616 1105 22-23 0.3423446162620749 1105 24-25 0.4958729870762042 1105 26-27 0.5373033256778115 1105 28-29 0.4050149886398273 1105 30-31 0.19153784557371267 1105 32-33 0.241934835260885 1105 34-35 0.2531823866769116 1105 36-37 0.3189820608613445 1105 38-39 0.2861368187532136 1105 40-41 0.15910904550352 1105 42-43 0.5308077843932182 1105 44-45 0.2051934866767482 1105 46-47 0.3973368170077052 1105 48-49 0.34066527670460545 1105 50-51 0.49734598892974446 1105 52-53 0.3617267069498453 1105 54-55 0.40075409004980855 1105 56-57 0.6201085222987288 1105 58-59 0.6571172546341444 1105 60-61 0.5042749307471937 1105 62-63 0.5946364600214267 1105 64-65 0.7315227134719358 1105 66-67 0.6757413136635293 1105 68-69 0.6077072762262041 1105 70-71 0.5377263008602817 1105 72-73 0.6292173754214119 1105 74-75 0.5973364869788291 1105 76-77 0.42685257392992426 1105 78-79 0.3491036663313061 1105 80-81 0.13793435934568166 1105 82-83 0.4118053149314598 1105 84-85 0.5198316949729573 1105 86-87 0.5667385504555504 1105 88-89 0.4973476771417715 1105 90-91 0.4589196090002865 1105 92-93 0.5845085937339967 1105 94-95 0.45766366253209156 1105 96-97 0.5998401534871434 1105 98-99 0.5641504603737708 1105 100-101 1.3891143562938062 1106 1 0.1690515297553432 1106 2 0.09374890824704352 1106 3 0.21587632876504514 1106 4 0.19557514034737267 1106 5 0.25548215911663164 1106 6 0.08686174285843151 1106 7 0.36903515684320354 1106 8 0.3399098071732993 1106 9 0.341449971291496 1106 10-11 0.36151893212242925 1106 12-13 0.48943472566146795 1106 14-15 0.5247250148622769 1106 16-17 0.5595225837356139 1106 18-19 0.4656988641143016 1106 20-21 0.4284703162422403 1106 22-23 0.2352286065571647 1106 24-25 0.18315062145615713 1106 26-27 0.012455700934616232 1106 28-29 0.3306842158098817 1106 30-31 0.16031803787485188 1106 32-33 0.09115878273286881 1106 34-35 -0.047361423339729924 1106 36-37 -0.011564723842568014 1106 38-39 0.06804405808848912 1106 40-41 0.03542976017212851 1106 42-43 -0.10494206919639026 1106 44-45 0.11215100132553601 1106 46-47 0.31880755197236255 1106 48-49 0.23108972739451872 1106 50-51 0.04146788293791559 1106 52-53 0.21627456997789096 1106 54-55 0.4045644785253728 1106 56-57 0.24167485007827594 1106 58-59 0.27722102783902614 1106 60-61 0.33911208211230104 1106 62-63 0.43327625328065267 1106 64-65 0.3301137652407924 1106 66-67 0.6974326712674213 1106 68-69 0.5043152031480496 1106 70-71 0.692253093186828 1106 72-73 0.7665602360170318 1106 74-75 0.45321319858599907 1106 76-77 0.7200506106363278 1106 78-79 0.7307873393264153 1106 80-81 0.75148932753822 1106 82-83 0.9709301681337443 1106 84-85 0.8937921812103831 1106 86-87 0.7777342030740506 1106 88-89 1.0706404080596208 1106 90-91 1.0634490351645205 1106 92-93 1.061520458398121 1106 94-95 0.907322503085755 1106 96-97 1.023670497156484 1106 98-99 1.295571059463395 1106 100-101 1.8722460202904863 1107 1 0.3008341199530129 1107 2 0.26176626433962724 1107 3 0.18452733914189068 1107 4 0.44351561305346365 1107 5 0.35994489571861976 1107 6 0.2468974688904595 1107 7 0.4565126394856023 1107 8 0.4863943837262781 1107 9 0.5243980327619937 1107 10-11 0.418367980584911 1107 12-13 0.44207374488533446 1107 14-15 0.41767470572951026 1107 16-17 0.4488058302180775 1107 18-19 0.642943131806085 1107 20-21 0.5975522115323812 1107 22-23 0.6800119722269784 1107 24-25 0.6176983582542732 1107 26-27 0.4953962189017247 1107 28-29 0.5323213069142767 1107 30-31 0.6054509694914785 1107 32-33 0.32300926446099254 1107 34-35 0.5908394812771931 1107 36-37 0.5301820799750558 1107 38-39 0.3499473443403076 1107 40-41 0.4340497516630606 1107 42-43 0.6149260100164327 1107 44-45 0.7920609922431581 1107 46-47 0.5879911336881136 1107 48-49 0.7644587163538503 1107 50-51 0.618607597672792 1107 52-53 0.8757570554717304 1107 54-55 0.8556572304255852 1107 56-57 0.9133642327481297 1107 58-59 0.774733934773316 1107 60-61 0.8240385027802901 1107 62-63 0.9017551209102805 1107 64-65 0.933336953366716 1107 66-67 0.9450041157879667 1107 68-69 1.012359595156763 1107 70-71 1.0333629235786965 1107 72-73 0.8987694867661027 1107 74-75 0.7114629247568267 1107 76-77 0.7692893202748117 1107 78-79 0.764332695394863 1107 80-81 1.1246782078107493 1107 82-83 1.1719701155102662 1107 84-85 1.0024396008796153 1107 86-87 1.0260767445105152 1107 88-89 1.0241731815644215 1107 90-91 1.1748715588586656 1107 92-93 1.2154791381039836 1107 94-95 1.3644007231810455 1107 96-97 1.2352322739230956 1107 98-99 1.5307103577968846 1107 100-101 1.9521335315680233 1108 1 -0.0958712724938664 1108 2 0.12445253821320534 1108 3 -0.07140851298953521 1108 4 0.06977701100854006 1108 5 0.05489408803273932 1108 6 -0.006743099141559128 1108 7 -0.04038134289144324 1108 8 -0.059442915838161525 1108 9 0.007037842977268838 1108 10-11 -0.0757200480635305 1108 12-13 0.18374374980705 1108 14-15 0.10071671376497449 1108 16-17 0.20421736656037837 1108 18-19 0.1426162304638794 1108 20-21 0.03672233093021049 1108 22-23 0.3306363143683555 1108 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86-87 -2.7088939299762878 2316 88-89 -2.8066865957154334 2316 90-91 -2.7249370084835114 2316 92-93 -3.090087584245385 2316 94-95 -3.4424875291561072 2316 96-97 -4.478733060789757 2316 98-99 -5.615470158274487 2316 100-101 -6.04292709744902 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4530.0 3 1964.0 4 2001.0 5 2203.0 6 2475.0 7 2978.0 8 3856.0 9 4489.0 10 5906.0 11 7491.0 12 9636.0 13 11749.0 14 13223.0 15 16079.0 16 19681.0 17 24726.0 18 20159.0 19 13478.0 20 13410.0 21 13894.0 22 15312.0 23 17095.0 24 19559.0 25 22292.0 26 25798.0 27 30101.0 28 35285.0 29 42165.0 30 49837.0 31 60480.0 32 74277.0 33 93126.0 34 120395.0 35 160265.0 36 213630.0 37 264344.0 38 203488.0 39 30933.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.25926475356001 18.885717259472916 14.67825042499967 21.176767561967406 2 15.593847846955772 20.705051332737217 40.42172692120208 23.27937389910493 3 19.361884739720868 26.73229841712247 29.7147351453837 24.19108169777297 4 12.411651317198544 17.072848252649415 37.84129483542347 32.67420559472857 5 13.123570793511 38.48671339047567 33.138504542947096 15.25121127306624 6 29.85795033047607 36.6043524248472 18.257666039695415 15.28003120498131 7 27.788892412068417 34.39068604282458 20.412391487732357 17.408030057374646 8 27.179371829791155 33.10321682538524 19.90426036586132 19.813150978962284 9 27.862201318738116 16.566782204812398 20.603697779011462 34.967318697438024 10-11 24.89585383700011 27.108843883361082 26.54493197219897 21.45037030743983 12-13 26.76747024964187 24.960597582640382 27.287591132579074 20.98434103513867 14-15 23.640367354459247 25.584280713173023 26.93164986468281 23.843702067684916 16-17 21.42057586677061 29.158946384095845 27.250079720580118 22.17039802855343 18-19 21.39377435608108 26.7857586234026 31.01230057815918 20.808166442357138 20-21 22.996708950542157 25.512062297299643 30.781718438548637 20.709510313609563 22-23 22.274363210897373 25.814514380555785 30.706011647988518 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22.227753514837467 27.04670533787677 28.655205230390298 22.070335916895466 50-51 22.222713889001838 27.340197665991354 28.475300122704084 21.961788322302723 52-53 22.409815429970728 27.380738100719682 28.254642655320776 21.95480381398881 54-55 21.984977324856306 27.299096911234894 28.439925595269244 22.27600016863956 56-57 22.262332917374263 27.629102063677323 27.899520316356163 22.209044702592255 58-59 22.451731777323737 27.11064052332923 28.078352267647368 22.35927543169967 60-61 22.161318767935512 27.2660754669251 28.614470170347932 21.958135594791454 62-63 22.188770570573084 27.31273370724499 28.534843571253017 21.963652150928908 64-65 22.00303172548234 27.39190882100561 28.41339699757522 22.191662455936832 66-67 22.026376000028705 27.602347084861755 28.08518348859767 22.28609342651187 68-69 22.15063294016045 27.49581648263975 28.26185768505261 22.09169289214719 70-71 22.199185570989847 27.387096571641976 28.085332354192598 22.32838550317558 72-73 22.16397163866991 27.562210088400423 27.997843658676164 22.2759746142535 74-75 21.972014427229826 28.04779416051803 27.798460102813298 22.181731309438845 76-77 22.096227832833044 27.692898343717303 27.892125892195857 22.31874793125379 78-79 22.103395177084423 27.777930601444183 27.8857809206297 22.232893300841695 80-81 22.12552383828628 28.08389186991019 27.654385589831072 22.136198701972457 82-83 22.239677316633053 28.001572752511244 27.534740338859397 22.224009591996303 84-85 22.00245925071798 27.945419226890998 27.547824412369458 22.504297110021565 86-87 22.194850592061417 27.858146850606413 27.453063999681866 22.493938557650303 88-89 22.245973091451212 27.76573290767151 27.29297798524655 22.695316015630723 90-91 22.052133041512683 27.76715343307859 27.545866073457475 22.634847451951252 92-93 22.168714618371073 27.793143271692934 27.68306792345741 22.355074186478586 94-95 21.85933524943396 27.58006776920197 27.806573110242766 22.7540238711213 96-97 21.93520260548295 27.50483504628129 27.731846000935313 22.828116347300448 98-99 21.789656828530298 27.730817009706556 27.424590137461173 23.054936024301973 100-101 21.905246720369824 27.642838620451204 27.57066746504574 22.88124719413323 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 885.0 1 634.0 2 694.0 3 960.0 4 779.5 5 714.0 6 1101.5 7 1960.5 8 3058.5 9 3564.5 10 3116.5 11 2591.5 12 2612.5 13 2790.0 14 2961.5 15 3174.0 16 3366.5 17 3520.5 18 3650.0 19 3886.0 20 4225.0 21 4798.5 22 5537.0 23 6470.0 24 7551.0 25 8900.0 26 10969.0 27 13439.5 28 15546.5 29 18201.5 30 21547.0 31 25248.0 32 30088.5 33 33608.5 34 36224.0 35 38761.5 36 42030.5 37 45621.0 38 46568.5 39 46793.5 40 47652.5 41 49538.5 42 50892.0 43 52195.0 44 54459.0 45 56878.0 46 57433.5 47 58419.0 48 64302.0 49 63784.0 50 57263.5 51 53178.5 52 50689.0 53 50329.0 54 49369.0 55 46587.0 56 42720.0 57 39016.5 58 36987.0 59 34034.0 60 29440.5 61 25068.0 62 21558.5 63 17047.0 64 12225.5 65 9611.5 66 7540.5 67 5900.5 68 4512.0 69 3258.0 70 2584.5 71 2104.5 72 1734.5 73 1598.5 74 1305.0 75 1032.0 76 786.0 77 503.5 78 334.0 79 228.0 80 153.5 81 105.0 82 74.5 83 51.5 84 28.5 85 20.0 86 18.5 87 16.5 88 11.5 89 6.0 90 4.5 91 5.0 92 4.5 93 2.0 94 3.5 95 4.5 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.032948298677822445 2 0.0228425591559495 3 0.021646613650402404 4 0.04873477935104409 5 0.05591045238432665 6 0.04718005019383287 7 0.050648292159919436 8 0.0418580926941483 9 0.04299424092441804 10-11 0.05190403494074389 12-13 0.04679136790453006 14-15 0.0412003226660974 16-17 0.05106687308686093 18-19 0.05330927090976173 20-21 0.01231823870713507 22-23 0.021198134085822246 24-25 0.005800335701903407 26-27 0.010793408187562525 28-29 0.006428207092315631 30-31 0.009029388566880561 32-33 0.003348647415531864 34-35 0.008521111727023047 36-37 0.009447969493822045 38-39 0.011361482302697396 40-41 0.0018537155335979962 42-43 0.0016145264324885773 44-45 0.0023918910110941887 46-47 4.18580926941483E-4 48-49 8.072632162442887E-4 50-51 5.979727527735471E-5 52-53 0.003109458314422445 54-55 0.00666739619342505 56-57 0.016593743889465933 58-59 0.010554219086453107 60-61 0.010793408187562525 62-63 0.005142565673852505 64-65 1.1959455055470941E-4 66-67 0.006876686656895792 68-69 0.016683439802381964 70-71 0.006129220715928858 72-73 0.0023918910110941887 74-75 0.00373732970483467 76-77 0.006488004367592986 78-79 0.005501349325516634 80-81 0.00983665178312485 82-83 0.005351856137323247 84-85 0.015726683397944287 86-87 0.0039765188059440884 88-89 0.005830234339542084 90-91 0.0034084446908092185 92-93 0.016773135715297996 94-95 0.010225334072427657 96-97 0.009029388566880561 98-99 0.021168235448183567 100-101 0.03566907470294209 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1672317.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.90537539381003 #Duplication Level Percentage of deduplicated Percentage of total 1 77.61565827088297 42.615168538005065 2 12.845002706878503 14.105193911113403 3 4.063794281028813 6.69372451569316 4 1.834025542971807 4.027914436748134 5 0.9514216465320946 2.6119081330320744 6 0.5514489986413326 1.81665085685658 7 0.38895279189830867 1.494891933475298 8 0.27299736299766486 1.1991218157525612 9 0.19140402241739451 0.945819873244105 >10 1.121713068861955 11.634765998307518 >50 0.10625216295431937 4.0555145307025775 >100 0.05282653702899363 5.3264044523708804 >500 0.0032945904189246996 1.2201079314907963 >1k 8.785574450465867E-4 0.7649868706115015 >5k 2.1963936126164667E-4 0.8244999187153521 >10k+ 1.0981968063082334E-4 0.6633262838809119 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11001 0.6578298253261792 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8482 0.5072004889025227 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5192 0.31046745324002567 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2226 0.13310873476739157 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2214 0.13239116746406335 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 5.9797275277354714E-5 0.0 0.0 0.0 6 0.0 5.9797275277354714E-5 0.0 0.0 0.0 7 0.0 5.9797275277354714E-5 0.0 0.0 0.0 8 0.0 5.9797275277354714E-5 0.0 0.0 0.0 9 0.0 5.9797275277354714E-5 0.0 0.0 0.0 10-11 0.0 5.9797275277354714E-5 5.9797275277354714E-5 0.0 0.0 12-13 0.0 5.9797275277354714E-5 5.9797275277354714E-5 5.9797275277354714E-5 5.9797275277354714E-5 14-15 0.0 5.9797275277354714E-5 5.9797275277354714E-5 5.9797275277354714E-5 5.9797275277354714E-5 16-17 0.0 5.9797275277354714E-5 5.9797275277354714E-5 5.9797275277354714E-5 5.9797275277354714E-5 18-19 0.0 5.9797275277354714E-5 5.9797275277354714E-5 5.9797275277354714E-5 5.9797275277354714E-5 20-21 0.0 5.9797275277354714E-5 5.9797275277354714E-5 8.969591291603206E-5 5.9797275277354714E-5 22-23 0.0 5.9797275277354714E-5 5.9797275277354714E-5 2.3918910110941885E-4 5.9797275277354714E-5 24-25 0.0 5.9797275277354714E-5 5.9797275277354714E-5 2.989863763867736E-4 5.9797275277354714E-5 26-27 0.0 5.9797275277354714E-5 5.9797275277354714E-5 5.680741151348697E-4 5.9797275277354714E-5 28-29 0.0 5.9797275277354714E-5 5.9797275277354714E-5 9.866550420763527E-4 5.9797275277354714E-5 30-31 0.0 5.9797275277354714E-5 5.9797275277354714E-5 0.0020331073594300605 5.9797275277354714E-5 32-33 0.0 5.9797275277354714E-5 5.9797275277354714E-5 0.0037672283424733466 5.9797275277354714E-5 34-35 0.0 5.9797275277354714E-5 5.9797275277354714E-5 0.00621891662884489 5.9797275277354714E-5 36-37 0.0 5.9797275277354714E-5 5.9797275277354714E-5 0.00926857766798998 5.9797275277354714E-5 38-39 0.0 5.9797275277354714E-5 5.9797275277354714E-5 0.013394589662127455 5.9797275277354714E-5 40-41 0.0 5.9797275277354714E-5 5.9797275277354714E-5 0.01787938530792906 5.9797275277354714E-5 42-43 0.0 5.9797275277354714E-5 5.9797275277354714E-5 0.02239407959136934 1.1959455055470943E-4 44-45 0.0 5.9797275277354714E-5 5.9797275277354714E-5 0.0260118147456493 1.1959455055470943E-4 46-47 0.0 5.9797275277354714E-5 5.9797275277354714E-5 0.032380224562687576 1.1959455055470943E-4 48-49 0.0 5.9797275277354714E-5 5.9797275277354714E-5 0.03647633791918638 1.1959455055470943E-4 50-51 0.0 5.9797275277354714E-5 5.9797275277354714E-5 0.04191788996942565 1.1959455055470943E-4 52-53 0.0 5.9797275277354714E-5 5.9797275277354714E-5 0.05280099406990421 1.1959455055470943E-4 54-55 0.0 5.9797275277354714E-5 5.9797275277354714E-5 0.06981331888631162 1.1959455055470943E-4 56-57 0.0 5.9797275277354714E-5 5.9797275277354714E-5 0.09486837722752325 1.1959455055470943E-4 58-59 0.0 5.9797275277354714E-5 5.9797275277354714E-5 0.13182309334892844 1.1959455055470943E-4 60-61 0.0 5.9797275277354714E-5 5.9797275277354714E-5 0.176162772967087 1.1959455055470943E-4 62-63 0.0 5.9797275277354714E-5 5.9797275277354714E-5 0.20414789779688897 1.1959455055470943E-4 64-65 0.0 5.9797275277354714E-5 5.9797275277354714E-5 0.23889011473303207 1.1959455055470943E-4 66-67 0.0 5.9797275277354714E-5 5.9797275277354714E-5 0.2987770859233028 1.1959455055470943E-4 68-69 0.0 5.9797275277354714E-5 5.9797275277354714E-5 0.38353972362895317 1.7939182583206413E-4 70-71 0.0 5.9797275277354714E-5 5.9797275277354714E-5 0.4663290512504507 1.7939182583206413E-4 72-73 0.0 5.9797275277354714E-5 5.9797275277354714E-5 0.5556362818771801 1.7939182583206413E-4 74-75 0.0 5.9797275277354714E-5 5.9797275277354714E-5 0.6431495942455886 1.7939182583206413E-4 76-77 0.0 5.9797275277354714E-5 5.9797275277354714E-5 0.7262379082434729 1.7939182583206413E-4 78-79 0.0 5.9797275277354714E-5 5.9797275277354714E-5 0.8024495355844616 1.7939182583206413E-4 80-81 0.0 5.9797275277354714E-5 5.9797275277354714E-5 0.8821593035291754 1.7939182583206413E-4 82-83 0.0 5.9797275277354714E-5 5.9797275277354714E-5 0.9959535183819814 1.7939182583206413E-4 84-85 0.0 5.9797275277354714E-5 5.9797275277354714E-5 1.1754649387646001 1.7939182583206413E-4 86-87 0.0 5.9797275277354714E-5 5.9797275277354714E-5 1.4022760038916067 1.7939182583206413E-4 88-89 0.0 5.9797275277354714E-5 5.9797275277354714E-5 1.6675068183843136 1.7939182583206413E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14960 0.0 32.972256 1 GGTATCA 9785 0.0 30.74155 1 TCAACGC 18160 0.0 27.168669 4 ATCAACG 18105 0.0 27.11588 3 TATCAAC 18300 0.0 26.826937 2 CAACGCA 18350 0.0 26.75544 5 GTACATG 12385 0.0 26.244783 1 AACGCAG 18780 0.0 26.191093 6 TACATGG 12650 0.0 25.434315 2 TACCTGG 1215 0.0 25.424881 2 ACATGGG 12810 0.0 24.374634 3 GAGTACT 9505 0.0 24.178242 12-13 ACGCAGA 20825 0.0 23.55421 7 CGCAGAG 20895 0.0 23.496643 8 GTACCTG 1355 0.0 23.146616 1 GCAGAGT 21415 0.0 22.392105 9 AGTACTT 9795 0.0 22.346294 12-13 GTACTTT 10315 0.0 22.048882 14-15 GTACACG 325 1.8189894E-12 21.932634 1 GTATAGG 590 0.0 21.746763 1 >>END_MODULE