FastQCFastQC Report
Thu 26 May 2016
SRR936757_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936757_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1672317
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124960.7472267518658245No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106810.6386946972374257No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66300.39645593508886173No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28580.17090061274267979No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25240.15092832280004329No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19300.11540874128529459No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17580.10512360993758958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112100.031.1534541
GTATCAA164500.030.29941
TATAGCG953.493157E-529.9982725
TATCAAC204800.024.398012
ATCAACG203650.024.3491973
TCAACGC205150.024.2167454
CAACGCA206300.024.0824725
GTACATG119650.023.7472291
GAGTACT115100.023.58568212-13
AACGCAG211900.023.4684456
TACATGG121600.022.9674262
AGTACTT116500.022.03826112-13
TATAACG1954.2261672E-721.9218142
GTACTTT122750.021.88359514-15
ACATGGG122200.021.7274723
ACGCAGA235400.021.0852347
CGCAGAG237000.020.9428868
TAACGCA2108.619736E-720.3553624
GCAGAGT244200.020.0932069
ATGGGCG3153.2196112E-1019.6020455
Produced by FastQC (version 0.11.5)