##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936757_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1672317 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.717385519611412 31.0 31.0 34.0 30.0 34.0 2 31.837927259006516 31.0 31.0 34.0 30.0 34.0 3 31.921199748612256 31.0 31.0 34.0 30.0 34.0 4 35.72267458860969 37.0 35.0 37.0 35.0 37.0 5 35.4881143945795 37.0 35.0 37.0 33.0 37.0 6 35.597064432162085 37.0 35.0 37.0 33.0 37.0 7 35.53695142727126 37.0 35.0 37.0 33.0 37.0 8 35.513927682371225 37.0 35.0 37.0 33.0 37.0 9 37.0964589847499 39.0 37.0 39.0 33.0 39.0 10-11 36.963628606298926 39.0 37.0 39.0 33.0 39.0 12-13 36.85573578454324 39.0 37.0 39.0 32.5 39.0 14-15 37.85964533040088 40.0 37.0 41.0 33.0 41.0 16-17 37.79182206483579 39.0 37.0 41.0 32.5 41.0 18-19 37.99678230861733 40.0 37.0 41.0 33.0 41.0 20-21 38.05420533307979 40.0 37.0 41.0 33.0 41.0 22-23 37.947166117428694 40.0 37.0 41.0 33.0 41.0 24-25 37.81172229906173 40.0 37.0 41.0 32.0 41.0 26-27 37.56627541309453 39.0 36.5 41.0 32.0 41.0 28-29 37.447934811402384 39.0 36.0 41.0 32.0 41.0 30-31 37.329898577841405 39.0 36.0 41.0 32.0 41.0 32-33 37.10443893113566 39.0 35.5 41.0 31.0 41.0 34-35 36.893468463216 39.0 35.0 41.0 31.0 41.0 36-37 36.57610399224549 39.0 35.0 41.0 30.5 41.0 38-39 36.375991513570696 39.0 35.0 40.0 30.0 41.0 40-41 36.11019920266313 38.5 35.0 40.0 30.0 41.0 42-43 35.78480395762287 38.0 35.0 40.0 29.0 41.0 44-45 35.42851504828331 38.0 35.0 40.0 27.5 41.0 46-47 34.93808081840943 38.0 34.0 40.0 25.5 41.0 48-49 34.45767728247695 38.0 34.0 40.0 24.0 41.0 50-51 34.09000954962486 38.0 33.0 40.0 23.0 41.0 52-53 33.78616434563543 38.0 33.0 40.0 22.0 41.0 54-55 33.50226003801911 37.5 33.0 40.0 20.5 41.0 56-57 33.17209685723461 37.0 32.5 40.0 19.0 41.0 58-59 32.829155895682455 36.5 32.0 39.5 18.0 41.0 60-61 32.45906009446774 36.0 31.0 39.0 16.0 40.5 62-63 32.05477011834479 35.5 31.0 39.0 13.0 40.0 64-65 31.634446698801725 35.0 31.0 38.5 9.5 40.0 66-67 31.167692189937675 35.0 30.0 38.0 8.0 40.0 68-69 30.877613215676213 35.0 30.0 38.0 7.0 40.0 70-71 30.448579426029873 34.5 29.0 37.0 6.0 39.0 72-73 30.00938099654551 34.0 29.0 36.5 2.0 39.0 74-75 29.482797519848212 34.0 28.5 36.0 2.0 39.0 76-77 28.097242329056034 32.5 26.5 34.5 2.0 36.5 78-79 28.86369450289628 33.5 28.5 35.0 2.0 37.0 80-81 28.66417640913774 34.0 28.0 35.0 2.0 37.0 82-83 28.319449003986684 33.5 27.5 35.0 2.0 36.5 84-85 27.893965976546312 33.0 26.5 35.0 2.0 36.0 86-87 27.484513103675916 33.0 26.0 35.0 2.0 35.5 88-89 27.063480488448064 33.0 25.5 35.0 2.0 35.0 90-91 26.54879726750371 32.0 25.0 34.5 2.0 35.0 92-93 26.02887490828593 32.0 24.0 34.0 2.0 35.0 94-95 25.398938119985623 32.0 21.5 34.0 2.0 35.0 96-97 24.601428437311824 31.5 17.5 34.0 2.0 35.0 98-99 23.611977872616258 31.0 2.0 34.0 2.0 35.0 100-101 20.44270643663851 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3595583163255469 1101 2 0.4518561216181318 1101 3 0.4564440878055862 1101 4 0.263898351522009 1101 5 0.25691847203280815 1101 6 0.24145232346506873 1101 7 0.31389487739370736 1101 8 0.25724793692806713 1101 9 0.3955323615452784 1101 10-11 0.3937923037857587 1101 12-13 0.38061557049551453 1101 14-15 0.5195008470786817 1101 16-17 0.576284644420582 1101 18-19 0.3862268917405203 1101 20-21 0.40206616432345754 1101 22-23 0.32408586277102813 1101 24-25 0.24847021899925892 1101 26-27 0.4232587193228099 1101 28-29 0.3599829892092785 1101 30-31 0.3513773329033967 1101 32-33 0.486468882059782 1101 34-35 0.23200670194272277 1101 36-37 0.16286578913865668 1101 38-39 0.2793208806994443 1101 40-41 0.1912219495630083 1101 42-43 0.2635992786896395 1101 44-45 0.3517042305125244 1101 46-47 0.4607231690392197 1101 48-49 0.4999296012842862 1101 50-51 0.39170181630866097 1101 52-53 0.15854796854453213 1101 54-55 0.22459540425163738 1101 56-57 0.28313293877042156 1101 58-59 0.27683332983010445 1101 60-61 0.17770921891852254 1101 62-63 0.2516838171290914 1101 64-65 0.22730873677229013 1101 66-67 0.19900536581922879 1101 68-69 0.10871397460993748 1101 70-71 0.3163934178691754 1101 72-73 0.3090403095872567 1101 74-75 0.44366207855518525 1101 76-77 0.24865868293850113 1101 78-79 -0.01280540694187593 1101 80-81 0.33578233398498014 1101 82-83 0.3743712299469024 1101 84-85 0.33016770912788473 1101 86-87 0.38330636789997286 1101 88-89 0.2951785151336459 1101 90-91 0.35707969087216185 1101 92-93 0.3486460026505718 1101 94-95 0.2884979869844706 1101 96-97 0.4856242970350415 1101 98-99 0.8346502445425052 1101 100-101 1.361966123920208 1102 1 0.22407472491271108 1102 2 0.2491668257643873 1102 3 0.24148760947282 1102 4 0.11270744202142424 1102 5 0.0850995748590293 1102 6 0.23963900732547927 1102 7 0.18734475381366167 1102 8 0.2573058271584827 1102 9 0.24796323125066522 1102 10-11 0.32475283096816554 1102 12-13 0.24919140149764019 1102 14-15 0.4324178213955179 1102 16-17 0.42830178963492926 1102 18-19 0.2972818458616473 1102 20-21 0.21462054474200443 1102 22-23 0.12935370041493144 1102 24-25 0.1711763619499962 1102 26-27 -0.00851833742695618 1102 28-29 0.12371718638964069 1102 30-31 0.2889801573920039 1102 32-33 0.37885215698291574 1102 34-35 0.3939102505791823 1102 36-37 0.5085588599900674 1102 38-39 0.4978646467535839 1102 40-41 0.5059862115318978 1102 42-43 0.5430212083408819 1102 44-45 0.8652881557474643 1102 46-47 0.8469243219907483 1102 48-49 0.7113156043278579 1102 50-51 0.7092907402090063 1102 52-53 0.4598369794535415 1102 54-55 0.578601789509392 1102 56-57 0.6002752066115278 1102 58-59 0.450202783894035 1102 60-61 0.5832318429058745 1102 62-63 0.44254045388002083 1102 64-65 0.6618577649443829 1102 66-67 0.7367269172774051 1102 68-69 0.6743358439444052 1102 70-71 0.5070041078028602 1102 72-73 0.4614945780384687 1102 74-75 0.3972516194891256 1102 76-77 0.307658707203867 1102 78-79 0.2951061423324184 1102 80-81 0.6762593910813841 1102 82-83 0.6264370792173821 1102 84-85 0.6841754809779808 1102 86-87 0.7773370878533861 1102 88-89 0.6029744572711486 1102 90-91 0.6515477073864737 1102 92-93 0.6867064026131366 1102 94-95 0.757937491693685 1102 96-97 0.7091982734699052 1102 98-99 0.6976656669158672 1102 100-101 0.8578054802640445 1103 1 0.16805254511087497 1103 2 0.22137541286253182 1103 3 0.2511230971557623 1103 4 0.06931441714489495 1103 5 0.01193046902016448 1103 6 0.17897482528142206 1103 7 0.06566490652705426 1103 8 0.22102730702395235 1103 9 0.14932674365862653 1103 10-11 0.12262575161174993 1103 12-13 0.06980316406468035 1103 14-15 0.1353402829899224 1103 16-17 0.34888022189866774 1103 18-19 0.1440351713169008 1103 20-21 0.2818038311099116 1103 22-23 0.18503803214234438 1103 24-25 0.04780878930508692 1103 26-27 0.11032691906488168 1103 28-29 0.22368537110012454 1103 30-31 0.1472157723360965 1103 32-33 0.21536853428195002 1103 34-35 0.26899584885813255 1103 36-37 0.2874122975563509 1103 38-39 0.2737138119809899 1103 40-41 0.2886499087715464 1103 42-43 0.22761957818604373 1103 44-45 0.24857603866719558 1103 46-47 0.1822620185637902 1103 48-49 0.38408777655462245 1103 50-51 0.47595134682052986 1103 52-53 0.34116443453338974 1103 54-55 0.25677347087168556 1103 56-57 0.21516627712027514 1103 58-59 0.558680801043991 1103 60-61 0.41976034031520726 1103 62-63 0.42103483317887935 1103 64-65 0.36477568149741657 1103 66-67 0.649849966697154 1103 68-69 0.7340092069720114 1103 70-71 0.7777764618312837 1103 72-73 0.5169910480563473 1103 74-75 0.47344620785987956 1103 76-77 0.2947401846453097 1103 78-79 0.4933881166983838 1103 80-81 0.6084110136611507 1103 82-83 0.6405630677638783 1103 84-85 0.49384732899179795 1103 86-87 0.41304596280414785 1103 88-89 0.4001459265315219 1103 90-91 0.27855888818822194 1103 92-93 0.5972796838299921 1103 94-95 0.6531591382106647 1103 96-97 0.5705426967041554 1103 98-99 0.9373590281254529 1103 100-101 1.0863225847696967 1104 1 0.14514894289614588 1104 2 0.1571263218199661 1104 3 0.14623269365979752 1104 4 0.07823591645134798 1104 5 0.046169475561569584 1104 6 0.13246054663235896 1104 7 0.021280544072311613 1104 8 7.553345959792068E-4 1104 9 0.08747223866853915 1104 10-11 0.08988686291604608 1104 12-13 0.1524332869719487 1104 14-15 0.14565323094848992 1104 16-17 0.26093062122172483 1104 18-19 0.10476840341884497 1104 20-21 0.0990744087084039 1104 22-23 0.14941433858754039 1104 24-25 0.13960624538341193 1104 26-27 0.09546435420144661 1104 28-29 0.16098807614198307 1104 30-31 0.13329017740848315 1104 32-33 0.1573913461190699 1104 34-35 0.12120429665634447 1104 36-37 0.4340857644996632 1104 38-39 0.32006822617193365 1104 40-41 0.24863211600224844 1104 42-43 0.11705374493964626 1104 44-45 0.2233261478193711 1104 46-47 0.34453313709338573 1104 48-49 0.4586271157686497 1104 50-51 0.3244365465593333 1104 52-53 0.30954556299293046 1104 54-55 0.3406941008245923 1104 56-57 0.41957737702638553 1104 58-59 0.31517197420762955 1104 60-61 0.26455175342042025 1104 62-63 0.2852736851685336 1104 64-65 0.5375524546888748 1104 66-67 0.3661678294011139 1104 68-69 0.7003405929533955 1104 70-71 0.2842922666788006 1104 72-73 0.06022882977519117 1104 74-75 0.36988398339665807 1104 76-77 0.2871251305328819 1104 78-79 0.2879168884370529 1104 80-81 0.6924060216398829 1104 82-83 0.5248622528898181 1104 84-85 0.6119905443635147 1104 86-87 0.42243304923334435 1104 88-89 0.7494980649480603 1104 90-91 0.771705484379293 1104 92-93 0.6528579391726552 1104 94-95 0.7695892441172454 1104 96-97 0.7082062800635711 1104 98-99 1.0337041692118767 1104 100-101 1.331847828864852 1105 1 0.17279476768181112 1105 2 0.20029000668359842 1105 3 0.16379477952920496 1105 4 0.14832616727913717 1105 5 0.01846373868043827 1105 6 0.15747870846653456 1105 7 0.09629707479184191 1105 8 0.14187090408711356 1105 9 0.02181077776698004 1105 10-11 0.03701647755893589 1105 12-13 0.022594365816267725 1105 14-15 0.1484347519252509 1105 16-17 0.24644569585776566 1105 18-19 0.1597103043700372 1105 20-21 0.19569505284812294 1105 22-23 0.17741728337681906 1105 24-25 0.19328575770523315 1105 26-27 0.17046002547413508 1105 28-29 0.06958590098787454 1105 30-31 0.14721189770746435 1105 32-33 0.219250427851712 1105 34-35 0.16384084918262687 1105 36-37 0.061972680576303674 1105 38-39 0.18772256412131583 1105 40-41 0.26005321208619847 1105 42-43 0.17561624988004354 1105 44-45 0.3294514851777848 1105 46-47 0.38451505054141677 1105 48-49 0.4019054178033983 1105 50-51 0.48171614846698674 1105 52-53 0.5186787698521442 1105 54-55 0.6093935754802047 1105 56-57 0.2904624198305896 1105 58-59 0.4105924943826267 1105 60-61 0.42298693731604686 1105 62-63 0.42773116012779155 1105 64-65 0.3579524604605133 1105 66-67 0.5339042876991584 1105 68-69 0.5532258151802196 1105 70-71 0.40966930510158406 1105 72-73 0.32648508290411016 1105 74-75 0.13267568629289173 1105 76-77 0.06488058504297811 1105 78-79 0.34687807543681615 1105 80-81 0.5231000860137058 1105 82-83 0.3342368647159013 1105 84-85 0.22389630106896163 1105 86-87 0.1980807585837745 1105 88-89 0.18496568042156625 1105 90-91 0.22538590636120048 1105 92-93 0.32623617207324074 1105 94-95 0.35097668905144275 1105 96-97 0.3572598885283007 1105 98-99 0.47285433966335333 1105 100-101 0.5321723893108263 1106 1 0.08333290720084463 1106 2 0.16771873038782914 1106 3 0.13055419859756512 1106 4 0.006151918775003651 1106 5 -0.16457248743298436 1106 6 -0.0939883391253602 1106 7 0.08861773368507642 1106 8 -0.15807332052656875 1106 9 0.05152046688117906 1106 10-11 0.08545523153681245 1106 12-13 -0.017725660955910882 1106 14-15 0.07972970146401792 1106 16-17 0.07459754744566283 1106 18-19 -0.03171237080340461 1106 20-21 0.026196907379720358 1106 22-23 -0.031187154484747737 1106 24-25 -0.05582276570338962 1106 26-27 -0.01732903821206122 1106 28-29 0.025169481953589923 1106 30-31 0.12405333758004389 1106 32-33 0.18905053506439629 1106 34-35 0.061761046999329494 1106 36-37 -0.02038850843099027 1106 38-39 0.22033241839262274 1106 40-41 0.16896317511950798 1106 42-43 0.2742722180013075 1106 44-45 0.1989203485683788 1106 46-47 -0.04920504378095103 1106 48-49 0.05160670962332148 1106 50-51 0.08493934606988773 1106 52-53 0.02684289202502299 1106 54-55 -1.9049506946089423E-4 1106 56-57 0.10589376497797787 1106 58-59 -0.04410596218848184 1106 60-61 0.1516480491673846 1106 62-63 0.11532485503022372 1106 64-65 0.20777227497636375 1106 66-67 0.1402970765614313 1106 68-69 0.1258349877523628 1106 70-71 0.14390113859346698 1106 72-73 0.08700587008073057 1106 74-75 0.2125063770090776 1106 76-77 -0.027873262684021682 1106 78-79 0.0977167203428202 1106 80-81 0.17905736206778045 1106 82-83 0.4303287143826431 1106 84-85 0.2323645540289334 1106 86-87 0.31568641420794563 1106 88-89 0.26008892853217347 1106 90-91 0.4605377238641317 1106 92-93 0.5118154459396855 1106 94-95 0.6879866015770482 1106 96-97 0.6446397777831763 1106 98-99 1.0037478613522346 1106 100-101 1.1615791870909646 1107 1 0.04907272875172808 1107 2 0.14037956542347985 1107 3 0.0977927634007898 1107 4 0.05794651823006092 1107 5 -0.058344872779272805 1107 6 0.117155527553777 1107 7 0.07388104721419353 1107 8 0.007069960776711071 1107 9 0.07930168114383207 1107 10-11 0.048654702888086376 1107 12-13 0.07817006972290841 1107 14-15 0.18707797898045442 1107 16-17 0.08452917313098851 1107 18-19 0.21292344394021256 1107 20-21 0.08178241902538019 1107 22-23 0.0430836150431162 1107 24-25 0.05931629123799098 1107 26-27 0.03529579922544457 1107 28-29 0.00830794144950886 1107 30-31 -0.09853553243332414 1107 32-33 0.23678250533696144 1107 34-35 0.17572075262165043 1107 36-37 0.20432476300538838 1107 38-39 0.2786492817435757 1107 40-41 0.19837061784168242 1107 42-43 0.15174203644426854 1107 44-45 0.25980637830184605 1107 46-47 0.4214994590870873 1107 48-49 0.20464523278639746 1107 50-51 0.19522083944216462 1107 52-53 0.4076849582207771 1107 54-55 0.4301621486267351 1107 56-57 0.07415025012784326 1107 58-59 0.25074861415328087 1107 60-61 0.36394365794319583 1107 62-63 0.41639633425089073 1107 64-65 0.5664166772442449 1107 66-67 0.6110409565171366 1107 68-69 0.6568280748858584 1107 70-71 0.5333329920925927 1107 72-73 0.5771484724686999 1107 74-75 0.5310552483500999 1107 76-77 0.4878642958158501 1107 78-79 0.5684198653802532 1107 80-81 0.8584373464376434 1107 82-83 0.7553983409188767 1107 84-85 0.7309198222608231 1107 86-87 0.706962352544334 1107 88-89 0.5179998005309905 1107 90-91 0.6640348920329622 1107 92-93 0.5177147673261189 1107 94-95 0.8316980354159931 1107 96-97 1.1670494141561392 1107 98-99 1.3371771983386331 1107 100-101 1.510637154388366 1108 1 -0.08481247550551174 1108 2 0.018371069021061004 1108 3 -0.04355870982347554 1108 4 -0.062421709749358456 1108 5 -0.20244892808442927 1108 6 0.010095641229320051 1108 7 0.015083079824876222 1108 8 0.08638356499743338 1108 9 0.09668567589642407 1108 10-11 -0.023996239561199673 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92.0 6 205.0 7 526.0 8 1082.0 9 1985.0 10 3241.0 11 4985.0 12 7128.0 13 9709.0 14 12190.0 15 16500.0 16 20666.0 17 26030.0 18 22096.0 19 14206.0 20 12528.0 21 12813.0 22 13380.0 23 15075.0 24 17494.0 25 20715.0 26 25512.0 27 30987.0 28 38048.0 29 46292.0 30 55775.0 31 67414.0 32 82822.0 33 102345.0 34 130491.0 35 171842.0 36 230765.0 37 285776.0 38 162908.0 39 8636.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.60801011673245 19.869102211779005 15.539939916946125 20.98294775454242 2 15.886741633237891 21.60712876118578 39.38489856549289 23.121231040083433 3 19.29595539428268 26.741902536220618 30.355173741756623 23.606968327740077 4 12.835762704567092 17.89235501509288 37.16937987648238 32.10250240385765 5 12.896885313247658 38.63420683931082 33.294923228625436 15.173984618816089 6 29.719460747526632 37.349183616722925 17.626105129715395 15.305250506035048 7 27.38206930862988 34.61090211963402 20.39846512353818 17.60856344819792 8 27.19293052692761 33.567200476943064 19.511133355697513 19.728735640431807 9 27.400554090167322 16.404919957112725 20.513893813935493 35.68063213878446 10-11 24.882811869323348 27.31286226066802 26.36268014741277 21.441645722595855 12-13 26.764689437920325 25.062899954762862 27.369216679916892 20.803193927399924 14-15 23.398188938568264 25.714160137154146 27.146128128141296 23.74152279613629 16-17 21.055437418972122 29.19524070362096 27.645395703070808 22.10392617433611 18-19 21.004181623460145 26.616843576905573 31.68711434494776 20.691860454686523 20-21 22.55960519435167 25.2938364239066 31.591149163450815 20.555409218290915 22-23 21.95905087291742 25.519667737949263 31.58890374871173 20.93237764042159 24-25 21.59821563383419 25.775121426894955 31.942875167620688 20.683787771650163 26-27 21.306082151215236 25.89879895027375 31.87058957551388 20.92452932299713 28-29 20.986478247778493 26.059669369940703 31.997334101169606 20.956518281111197 30-31 21.14361264775336 25.80528458760615 31.89606870071524 21.15503406392525 32-33 21.04141165467824 25.900776591857834 31.671786340665918 21.38602541279801 34-35 21.64805112401916 25.84284243996869 31.49421499860361 21.01489143740854 36-37 21.902883497514555 25.499151982853068 31.35235411473708 21.245610404895295 38-39 21.543759579612374 25.78121495947484 31.429471028979695 21.245554431933098 40-41 21.5705456904774 25.874626633618075 31.373928487070085 21.18089918883444 42-43 21.566733859273445 26.08268099099029 30.811896380269893 21.538688769466372 44-45 21.604800588412928 26.193016184345463 30.53701649529539 21.66516673194622 46-47 22.044720601632527 26.56748886948134 29.76147158746565 21.62631894142048 48-49 22.271179873073542 26.734953570127974 29.147219978149945 21.84664657864854 50-51 22.18547640105029 27.044625392947076 28.91647964867527 21.853418557327366 52-53 22.43750855873068 27.127842470320267 28.669938867490817 21.764710103458235 54-55 22.032631877629115 27.073144405432046 28.78576633245837 22.108457384480467 56-57 22.287418876260173 27.333012017645252 28.35071766815704 22.028851437937536 58-59 22.536845180130495 26.868151466706212 28.440415798227768 22.154587554935517 60-61 22.182066839128506 27.043319974118763 28.962884824912962 21.81172836183977 62-63 22.294959621175494 26.99142549853474 28.903607796914947 21.81000708337482 64-65 22.069223082254737 27.125141807686948 28.70041066668899 22.10522444336932 66-67 22.054306051141477 27.282132056979076 28.443462541478226 22.220099350401224 68-69 22.234099044525426 27.134568948092934 28.619507276987164 22.011824730394473 70-71 22.257582376052845 27.055421061396608 28.549846006928714 22.137150555621833 72-73 22.25884844755633 27.17118768832607 28.384009079716005 22.18595478440159 74-75 22.094692822120706 27.667537571604473 28.146993763331935 22.09077584294289 76-77 22.084847122154276 27.426579435753812 28.325419423279723 22.163154018812193 78-79 22.137522809789306 27.489014075916124 28.1723434873959 22.20111962689867 80-81 22.09474773207035 27.79886738068328 27.97115229364382 22.135232593602556 82-83 22.245477962777194 27.68220831196712 27.900480048318784 22.1718336769369 84-85 22.024201966308848 27.58537381007697 27.846463936104083 22.543960287510103 86-87 22.11098187776386 27.455621301775146 27.77127292189289 22.6621238985681 88-89 22.211088844355377 27.424529751834314 27.632346160539523 22.732035243270783 90-91 22.039629984585183 27.383503005964084 27.921003174166504 22.655863835284233 92-93 22.10640341183618 27.378376794538767 27.98383180967963 22.531387983945425 94-95 21.809670828956815 27.222830674233595 28.07962678420704 22.88787171260255 96-97 21.966575923243322 27.15405004193333 27.954535601470432 22.924838433352917 98-99 21.707565459396815 27.388955531981306 27.67419859854161 23.229280410080264 100-101 21.628000849162945 27.421086151168943 27.823033245925366 23.127879753742743 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1043.0 1 796.5 2 1088.0 3 1366.0 4 854.5 5 667.0 6 1071.5 7 2113.0 8 3350.5 9 3985.0 10 3485.0 11 2743.0 12 2613.5 13 2685.0 14 2915.0 15 3184.0 16 3387.5 17 3691.0 18 4040.5 19 4340.0 20 4675.0 21 5192.5 22 5918.0 23 6883.0 24 7889.0 25 9225.0 26 11469.5 27 13790.5 28 15738.5 29 18615.5 30 22059.0 31 25820.5 32 30448.5 33 33671.0 34 36452.5 35 38919.0 36 42083.0 37 45945.5 38 46811.0 39 46535.0 40 47531.5 41 49638.0 42 50795.0 43 52226.0 44 54354.0 45 56406.0 46 56646.0 47 57884.5 48 64716.0 49 64214.0 50 56872.5 51 52819.0 52 50393.5 53 49726.0 54 48991.0 55 45989.0 56 42077.0 57 38664.0 58 36681.5 59 33685.5 60 28789.0 61 24599.5 62 21137.0 63 16382.0 64 11878.5 65 9516.5 66 7338.5 67 5612.5 68 4225.0 69 3009.5 70 2363.5 71 1953.5 72 1666.0 73 1590.0 74 1348.5 75 1074.0 76 809.5 77 544.0 78 347.5 79 239.5 80 179.0 81 126.0 82 98.5 83 66.0 84 34.5 85 16.5 86 7.0 87 4.0 88 5.5 89 5.0 90 1.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03773208070001082 2 0.006278713904122244 3 0.0038868228930280563 4 2.989863763867736E-4 5 8.37161853882966E-4 6 1.1959455055470941E-4 7 0.0 8 0.0 9 0.0015547291572112225 10-11 3.5878365166412826E-4 12-13 0.0011062495926310622 14-15 0.002272296460539479 16-17 0.0031692555896997996 18-19 0.0 20-21 2.9898637638677353E-5 22-23 0.0013454386937404809 24-25 8.670604915216434E-4 26-27 0.004275505182330862 28-29 0.005112667036213828 30-31 0.0011959455055470943 32-33 5.979727527735472E-4 34-35 0.008849996741048497 36-37 0.010823306825201202 38-39 0.008730402190493787 40-41 0.0019135128088753506 42-43 5.979727527735472E-4 44-45 0.0013155400561018038 46-47 2.9898637638677353E-5 48-49 5.979727527735472E-4 50-51 0.0010763509549923849 52-53 0.002870269213313026 54-55 0.003378546053170541 56-57 0.0015547291572112225 58-59 9.268577667989981E-4 60-61 0.0037672283424733466 62-63 0.005471450687877956 64-65 0.009447969493822045 66-67 0.0032290528649771546 68-69 0.003528039241363928 70-71 8.969591291603206E-5 72-73 0.0010763509549923849 74-75 0.006577700280509019 76-77 0.003019762401506413 78-79 0.003797126980112024 80-81 0.00520236294912986 82-83 0.005351856137323247 84-85 0.005830234339542084 86-87 0.0037074310671959924 88-89 0.010045942246595592 90-91 0.013424488299766134 92-93 0.001255742780824449 94-95 0.0024217896487328658 96-97 0.001166046867908417 98-99 0.003528039241363928 100-101 0.004903376572743086 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1672317.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.19000327666645 #Duplication Level Percentage of deduplicated Percentage of total 1 75.63668722956666 39.474789543472795 2 13.762923041766214 14.36573997292574 3 4.609434091639463 7.216991410387248 4 1.9872296063134613 4.148540786599525 5 1.0315328889583177 2.6917852427361906 6 0.6255437406393102 1.9588277924198272 7 0.4109074164037983 1.5011681585964542 8 0.3028607642074385 1.2645043421087938 9 0.21368485747176477 1.0036992070462865 >10 1.2394333379983806 12.321622657660331 >50 0.11360660373271511 4.128236017585748 >100 0.06041398541932793 5.659156162493346 >500 0.004364251271218892 1.537149747231931 >1k 0.0010336384589728956 0.9411708064353982 >5k 1.1484871766365507E-4 0.39739921326370453 >10k+ 2.2969743532731015E-4 1.3892189390366336 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12496 0.7472267518658245 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10681 0.6386946972374257 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6630 0.39645593508886173 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2858 0.17090061274267979 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2524 0.15092832280004329 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1930 0.11540874128529459 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1758 0.10512360993758958 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.9797275277354714E-5 5.9797275277354714E-5 14-15 0.0 0.0 0.0 5.9797275277354714E-5 5.9797275277354714E-5 16-17 0.0 0.0 0.0 5.9797275277354714E-5 5.9797275277354714E-5 18-19 0.0 0.0 0.0 5.9797275277354714E-5 5.9797275277354714E-5 20-21 0.0 0.0 0.0 8.969591291603206E-5 1.1959455055470943E-4 22-23 0.0 0.0 0.0 2.3918910110941885E-4 1.1959455055470943E-4 24-25 0.0 0.0 0.0 2.989863763867736E-4 1.1959455055470943E-4 26-27 0.0 0.0 0.0 5.680741151348697E-4 1.7939182583206413E-4 28-29 0.0 0.0 0.0 9.866550420763527E-4 1.7939182583206413E-4 30-31 0.0 0.0 0.0 0.0020630059970687376 1.7939182583206413E-4 32-33 0.0 0.0 0.0 0.003946620168305411 1.7939182583206413E-4 34-35 0.0 0.0 0.0 0.006428207092315632 1.7939182583206413E-4 36-37 0.0 0.0 0.0 0.009537665406738076 1.7939182583206413E-4 38-39 0.0 0.0 0.0 0.013992562414901002 1.7939182583206413E-4 40-41 0.0 0.0 0.0 0.018686648524173347 1.7939182583206413E-4 42-43 0.0 0.0 0.0 0.023261140082890985 1.7939182583206413E-4 44-45 0.0 0.0 0.0 0.026878875237170943 1.7939182583206413E-4 46-47 0.0 0.0 0.0 0.03306789322837715 1.7939182583206413E-4 48-49 0.0 0.0 0.0 0.037104209309598596 2.3918910110941885E-4 50-51 0.0 0.0 0.0 0.042784950460947296 2.3918910110941885E-4 52-53 0.0 0.0 0.0 0.05372785183670321 3.5878365166412826E-4 54-55 0.0 0.0 0.0 0.07082987256602666 3.5878365166412826E-4 56-57 0.0 0.0 0.0 0.09621381592126374 3.5878365166412826E-4 58-59 0.0 0.0 0.0 0.13325822795558498 3.5878365166412826E-4 60-61 0.0 0.0 0.0 0.17819588032651706 3.5878365166412826E-4 62-63 0.0 0.0 0.0 0.20653978880798318 3.5878365166412826E-4 64-65 0.0 0.0 0.0 0.24182018122162244 3.5878365166412826E-4 66-67 0.0 0.0 0.0 0.3018865442377253 3.5878365166412826E-4 68-69 0.0 0.0 0.0 0.3862903982917114 3.5878365166412826E-4 70-71 0.0 0.0 0.0 0.4696776986659826 3.5878365166412826E-4 72-73 0.0 0.0 0.0 0.5597024965960401 3.886822893028056E-4 74-75 0.0 0.0 0.0 0.6481127680936091 4.18580926941483E-4 76-77 0.0 0.0 0.0 0.7321578384959311 4.18580926941483E-4 78-79 0.0 0.0 0.0 0.8085787563003904 4.18580926941483E-4 80-81 0.0 0.0 0.0 0.8877204501299694 4.18580926941483E-4 82-83 0.0 0.0 0.0 1.002112637735549 4.18580926941483E-4 84-85 0.0 0.0 0.0 1.1809662880901168 4.18580926941483E-4 86-87 0.0 0.0 0.0 1.4083753259698968 4.18580926941483E-4 88-89 0.0 0.0 0.0 1.673486545912049 4.18580926941483E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11210 0.0 31.153454 1 GTATCAA 16450 0.0 30.2994 1 TATAGCG 95 3.493157E-5 29.998272 5 TATCAAC 20480 0.0 24.39801 2 ATCAACG 20365 0.0 24.349197 3 TCAACGC 20515 0.0 24.216745 4 CAACGCA 20630 0.0 24.082472 5 GTACATG 11965 0.0 23.747229 1 GAGTACT 11510 0.0 23.585682 12-13 AACGCAG 21190 0.0 23.468445 6 TACATGG 12160 0.0 22.967426 2 AGTACTT 11650 0.0 22.038261 12-13 TATAACG 195 4.2261672E-7 21.921814 2 GTACTTT 12275 0.0 21.883595 14-15 ACATGGG 12220 0.0 21.727472 3 ACGCAGA 23540 0.0 21.085234 7 CGCAGAG 23700 0.0 20.942886 8 TAACGCA 210 8.619736E-7 20.355362 4 GCAGAGT 24420 0.0 20.093206 9 ATGGGCG 315 3.2196112E-10 19.602045 5 >>END_MODULE