FastQCFastQC Report
Thu 26 May 2016
SRR936756_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936756_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1683961
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107940.6409887164845266No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83050.4931824430613298No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49870.2961470010291212No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22430.1331978590953116No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21800.12945667981621903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA152500.031.8164221
GGTATCA100900.031.7441831
GTACATG124900.026.900011
ATCAACG183950.026.1763063
TCAACGC184150.026.1220654
TACATGG127750.026.003072
TATCAAC185850.025.954452
CAACGCA186100.025.7221725
AACGCAG190850.025.281246
ACATGGG128600.024.3950463
GAGTACT94350.024.05351312-13
TATCTCG1000.001646644623.7681035
ACGCAGA209200.022.9728037
CGCAGAG211000.022.7331228
TATAGCG1050.002186936822.636295
GTACTTT101150.022.2703614-15
AGTACTT95700.021.65317512-13
GCAGAGT216800.021.554199
CATGGGA78350.021.1731344
CATGGGG44200.019.8951954
Produced by FastQC (version 0.11.5)