##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936756_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1683961 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.133544660476105 31.0 31.0 34.0 28.0 34.0 2 31.157067770571885 31.0 31.0 34.0 28.0 34.0 3 31.08422522849401 31.0 31.0 34.0 27.0 34.0 4 34.77121144729599 37.0 35.0 37.0 32.0 37.0 5 34.819583113860716 37.0 35.0 37.0 32.0 37.0 6 34.886812699343984 37.0 35.0 37.0 32.0 37.0 7 34.7565228648407 37.0 35.0 37.0 32.0 37.0 8 34.73380974975073 37.0 35.0 37.0 32.0 37.0 9 36.16351922639539 39.0 35.0 39.0 31.0 39.0 10-11 36.028942178589645 39.0 35.0 39.0 30.0 39.0 12-13 35.90565903842191 38.5 35.0 39.0 30.0 39.0 14-15 36.78928846926978 39.0 36.0 41.0 30.0 41.0 16-17 36.71556348395242 39.0 36.0 41.0 30.0 41.0 18-19 36.64281298676157 39.0 36.0 41.0 30.0 41.0 20-21 36.647621292892175 39.0 36.0 41.0 30.0 41.0 22-23 36.62384075403172 39.0 36.0 41.0 30.0 41.0 24-25 36.715066144643494 39.0 36.0 41.0 30.0 41.0 26-27 36.61986322723626 39.0 36.0 41.0 30.0 41.0 28-29 36.48724287557728 39.0 35.5 41.0 30.0 41.0 30-31 36.236982923001186 39.0 35.0 40.5 29.5 41.0 32-33 36.00522102352727 38.0 35.0 40.0 29.0 41.0 34-35 35.77178212559555 38.0 35.0 40.0 28.5 41.0 36-37 35.52267956324404 38.0 35.0 40.0 27.0 41.0 38-39 35.29212879633198 38.0 34.5 40.0 26.5 41.0 40-41 35.01296288928307 38.0 34.0 40.0 25.5 41.0 42-43 34.672906320277015 38.0 33.5 40.0 24.0 41.0 44-45 34.40806170689226 38.0 33.0 40.0 23.5 41.0 46-47 34.09189553677312 38.0 33.0 40.0 22.5 41.0 48-49 33.75068543748935 38.0 33.0 40.0 19.5 41.0 50-51 32.74206291000801 37.0 31.5 39.5 16.5 40.5 52-53 32.82715365735905 37.0 32.5 39.5 15.5 40.0 54-55 33.245343864851975 38.0 33.0 40.0 15.0 41.0 56-57 33.09927723979356 37.5 32.5 40.0 13.5 41.0 58-59 32.866921799257824 37.0 32.0 40.0 12.5 41.0 60-61 32.57544117708189 37.0 32.0 40.0 8.5 41.0 62-63 32.23818039728948 36.0 31.0 39.5 8.0 41.0 64-65 31.87340146238541 36.0 31.0 39.0 7.0 41.0 66-67 31.516988516954967 35.0 31.0 39.0 4.5 40.5 68-69 31.082085333330166 35.0 30.0 38.5 2.0 40.0 70-71 30.69607461217926 35.0 30.0 38.0 2.0 40.0 72-73 30.28824242366658 35.0 29.5 37.0 2.0 39.5 74-75 29.884485151378207 34.5 29.0 36.5 2.0 39.0 76-77 29.430448804930755 34.0 29.0 36.0 2.0 39.0 78-79 28.973010954529233 34.0 28.5 36.0 2.0 38.0 80-81 28.538735457650148 34.0 27.5 35.0 2.0 37.0 82-83 28.143941872763087 34.0 27.0 35.0 2.0 37.0 84-85 27.758917813417295 34.0 26.0 35.0 2.0 36.0 86-87 27.403899793403767 33.0 26.0 35.0 2.0 36.0 88-89 27.044162246037764 33.0 25.5 35.0 2.0 35.5 90-91 26.67988183811858 33.0 25.0 35.0 2.0 35.0 92-93 26.255584006993036 33.0 24.0 35.0 2.0 35.0 94-95 25.74962306134168 33.0 22.0 35.0 2.0 35.0 96-97 25.14198339510238 32.0 19.0 35.0 2.0 35.0 98-99 24.399647022704208 32.0 4.5 35.0 2.0 35.0 100-101 21.865999865792617 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5693385338822097 1101 2 0.621429628929608 1101 3 0.6564782612614621 1101 4 0.7802003397949022 1101 5 0.6796605709291867 1101 6 0.7414998774700194 1101 7 0.7952148356158162 1101 8 0.5646983291596825 1101 9 0.9391509143627985 1101 10-11 0.824795445289773 1101 12-13 0.9026817485434648 1101 14-15 1.1971531183385196 1101 16-17 1.217214639444336 1101 18-19 1.2810080531888772 1101 20-21 1.1867594265347847 1101 22-23 1.1229844388227832 1101 24-25 1.1738743179278401 1101 26-27 0.978879351707306 1101 28-29 0.9001877308551016 1101 30-31 0.9648490852144391 1101 32-33 0.9092071314825247 1101 34-35 1.098974886860539 1101 36-37 1.064253596090218 1101 38-39 1.0260069911979315 1101 40-41 0.9615538235374359 1101 42-43 1.1575524730469127 1101 44-45 1.0683668764293799 1101 46-47 1.0076404366151124 1101 48-49 1.067880635357504 1101 50-51 1.166648009416889 1101 52-53 1.2376244017364115 1101 54-55 1.2643189572186628 1101 56-57 1.2050311821331334 1101 58-59 1.3078368124054336 1101 60-61 1.140592349614444 1101 62-63 1.1754725067133478 1101 64-65 1.1237148198737223 1101 66-67 0.9832802028849628 1101 68-69 1.0432313436553677 1101 70-71 0.9387020770759626 1101 72-73 1.1011148985079728 1101 74-75 1.0432349534991836 1101 76-77 1.0141407946942387 1101 78-79 1.0792303674009922 1101 80-81 1.2274427497456806 1101 82-83 1.252442051188332 1101 84-85 1.0684608178020376 1101 86-87 1.2352484480958452 1101 88-89 1.2976431107891422 1101 90-91 1.2428720709133643 1101 92-93 1.0257189687085173 1101 94-95 1.4268215088352179 1101 96-97 1.5252038292652053 1101 98-99 1.477891349415902 1101 100-101 1.8311939977870466 1102 1 0.3999803742031318 1102 2 0.4952284858290348 1102 3 0.5767924364185504 1102 4 0.480059404724436 1102 5 0.5988000504989301 1102 6 0.5246451790426718 1102 7 0.7004361782264894 1102 8 0.6297535316872782 1102 9 0.7892893644320225 1102 10-11 0.7787515672678254 1102 12-13 0.6275425884738794 1102 14-15 0.765451424987674 1102 16-17 0.6707484887112614 1102 18-19 0.7147479800588386 1102 20-21 0.946383658846905 1102 22-23 0.4970035733323499 1102 24-25 0.9222320871067282 1102 26-27 0.7691022918187755 1102 28-29 0.6587597426411094 1102 30-31 0.5143678824738345 1102 32-33 0.7831923634751448 1102 34-35 0.4267408707435365 1102 36-37 0.5659893694581086 1102 38-39 0.6508536311212509 1102 40-41 0.9542385123797814 1102 42-43 0.7301153968283742 1102 44-45 0.7071781583350258 1102 46-47 0.6658391657144733 1102 48-49 0.9544532861438242 1102 50-51 0.8312138441997945 1102 52-53 0.674252255050348 1102 54-55 0.9014922683053186 1102 56-57 0.8419549280949994 1102 58-59 0.6181651875696161 1102 60-61 0.7173425004895222 1102 62-63 0.7171276950409435 1102 64-65 0.6592755426540826 1102 66-67 0.7230924077910714 1102 68-69 0.7253574397184117 1102 70-71 0.40488121016552725 1102 72-73 0.7304206956608752 1102 74-75 0.5813841600737852 1102 76-77 0.7503192177834528 1102 78-79 0.7313014384365282 1102 80-81 0.8468715544600833 1102 82-83 0.7561713280529716 1102 84-85 0.771057821600543 1102 86-87 0.7133607346519888 1102 88-89 0.6786356912854323 1102 90-91 0.8967697953622285 1102 92-93 0.8441972604476611 1102 94-95 0.9139527524008386 1102 96-97 0.9025065379165582 1102 98-99 1.0203016231210391 1102 100-101 1.5003224723512822 1103 1 0.48503453572788047 1103 2 0.4724491083437776 1103 3 0.5377124967503768 1103 4 0.43724300543744476 1103 5 0.518410635991863 1103 6 0.47703239966518396 1103 7 0.5906788767044802 1103 8 0.3174499529131154 1103 9 0.4278331735640677 1103 10-11 0.40734679556894093 1103 12-13 0.5469605234800525 1103 14-15 0.962566466808596 1103 16-17 0.9373445397190849 1103 18-19 0.8941919674440086 1103 20-21 1.0087468838571922 1103 22-23 0.7250283916512785 1103 24-25 0.6270819431330565 1103 26-27 0.7556297839345021 1103 28-29 0.7978170578748944 1103 30-31 0.6993822798411742 1103 32-33 0.909528576281943 1103 34-35 0.9687359345033002 1103 36-37 0.9059297232219876 1103 38-39 0.7490868684721335 1103 40-41 0.8968451214569768 1103 42-43 1.1319664869147559 1103 44-45 1.1161696570324011 1103 46-47 1.3822315968127867 1103 48-49 1.4406002764084676 1103 50-51 1.4160849534826738 1103 52-53 1.5082005616457081 1103 54-55 1.444544510181096 1103 56-57 1.3795101180031324 1103 58-59 1.4208760061627856 1103 60-61 1.3094753676223903 1103 62-63 1.2119556122218 1103 64-65 1.3675924218533133 1103 66-67 1.3685483244234575 1103 68-69 1.5609950374148305 1103 70-71 1.4492765207373104 1103 72-73 1.5269886375229333 1103 74-75 1.374987615441949 1103 76-77 1.3893196291368035 1103 78-79 1.2409264212968054 1103 80-81 1.3102707043298203 1103 82-83 1.1511253935952723 1103 84-85 1.3297428620502885 1103 86-87 1.3119772162096481 1103 88-89 1.3277910457349087 1103 90-91 1.3714626515256718 1103 92-93 1.4112677389887303 1103 94-95 1.4367657237432212 1103 96-97 1.4996503744721572 1103 98-99 1.804953343949233 1103 100-101 1.9566559428023993 1104 1 0.384940154401086 1104 2 0.44708256904926813 1104 3 0.6435232120152286 1104 4 0.6107777336992442 1104 5 0.5573673648754394 1104 6 0.5288949059452506 1104 7 0.41370109927650844 1104 8 0.42004980101098965 1104 9 0.6381241487678153 1104 10-11 0.674680264013837 1104 12-13 0.8478939738226146 1104 14-15 0.8148044323695842 1104 16-17 0.7888326689224598 1104 18-19 0.6972955652155832 1104 20-21 0.735729383630904 1104 22-23 0.7649718940314756 1104 24-25 0.7201436366449414 1104 26-27 0.5313191808253634 1104 28-29 0.4567134981444312 1104 30-31 0.5152585110944443 1104 32-33 0.7073642989910454 1104 34-35 0.6880623312354359 1104 36-37 0.7631791142361521 1104 38-39 0.6540280162382714 1104 40-41 0.8247891760892756 1104 42-43 0.8874517866459755 1104 44-45 0.9859965568255404 1104 46-47 1.1376091680316662 1104 48-49 1.0046282340070505 1104 50-51 0.925955311974306 1104 52-53 0.875249639935646 1104 54-55 1.1059301841582112 1104 56-57 1.206306445854615 1104 58-59 1.1016621586709476 1104 60-61 0.9130796980134406 1104 62-63 1.104529297212487 1104 64-65 1.205344263896098 1104 66-67 1.156490732793543 1104 68-69 1.0039859941227327 1104 70-71 1.1112170703396487 1104 72-73 1.1927559268833576 1104 74-75 1.1697146245049161 1104 76-77 1.1243133917051296 1104 78-79 0.9435365339378698 1104 80-81 1.055961891776775 1104 82-83 1.0560471928614703 1104 84-85 1.0566927317266632 1104 86-87 1.020598809397928 1104 88-89 1.1934498195253624 1104 90-91 1.178445951474643 1104 92-93 1.2514727412646387 1104 94-95 1.1906418185731695 1104 96-97 1.2570651131755852 1104 98-99 1.195846824882107 1104 100-101 1.6912430589314411 1105 1 0.3569831753235775 1105 2 0.2932116791063457 1105 3 0.5244394952420812 1105 4 0.4826084243322768 1105 5 0.5015451485381419 1105 6 0.42811856839841056 1105 7 0.573433377106042 1105 8 0.5523865849085681 1105 9 0.582146507289167 1105 10-11 0.5656703347748291 1105 12-13 0.6371644372133787 1105 14-15 0.9499472233070065 1105 16-17 0.8163087128008968 1105 18-19 0.6546359352409183 1105 20-21 0.651663770891723 1105 22-23 0.5964853660494356 1105 24-25 0.369935168339218 1105 26-27 0.4924356251521118 1105 28-29 0.620118286058478 1105 30-31 0.4445219440984829 1105 32-33 0.4073660329429316 1105 34-35 0.4485335878303687 1105 36-37 0.7149109380855521 1105 38-39 0.5058536311212478 1105 40-41 0.32187156560106445 1105 42-43 0.3779445284810308 1105 44-45 0.3932425841053302 1105 46-47 0.6403489696360438 1105 48-49 0.7406017455275773 1105 50-51 0.7729085220709493 1105 52-53 0.9238040757786337 1105 54-55 0.8111421282492941 1105 56-57 0.7053582894395376 1105 58-59 0.7954200895304027 1105 60-61 0.650465749789241 1105 62-63 0.7731360984022828 1105 64-65 0.8849618171638873 1105 66-67 0.9401512313204776 1105 68-69 0.8272341904186966 1105 70-71 0.9838728068041824 1105 72-73 0.8071854015432258 1105 74-75 0.8443393421466183 1105 76-77 1.010179161761041 1105 78-79 1.0689204860555748 1105 80-81 1.2595606300903341 1105 82-83 1.076759563347089 1105 84-85 1.0463659448498426 1105 86-87 1.2055596142038105 1105 88-89 1.1289438145347326 1105 90-91 1.1914896833174105 1105 92-93 1.4862280727725938 1105 94-95 1.481179643157141 1105 96-97 1.8856437928185166 1105 98-99 1.8505117071546557 1105 100-101 1.8985297552644482 1106 1 0.28050201682244236 1106 2 0.24033392423081068 1106 3 0.15274138203453091 1106 4 0.24635241249357875 1106 5 0.27642491176418815 1106 6 0.25419012909816274 1106 7 0.3600144246193864 1106 8 0.44367140072168354 1106 9 0.6039952467849616 1106 10-11 0.6761322553921403 1106 12-13 0.5764382599500166 1106 14-15 0.23803189113640144 1106 16-17 0.16852318349483397 1106 18-19 0.30816640403220674 1106 20-21 0.14583426927975296 1106 22-23 0.0018814867618743847 1106 24-25 -0.24919965540159694 1106 26-27 -0.09511524425225559 1106 28-29 -0.04238898099929145 1106 30-31 0.005261334194145206 1106 32-33 0.05854197501786729 1106 34-35 0.04121367873465687 1106 36-37 -0.1981116294320202 1106 38-39 0.11162499627108247 1106 40-41 -0.021749278963170582 1106 42-43 0.013439481179091217 1106 44-45 -0.08202827895687648 1106 46-47 0.2513552589442227 1106 48-49 0.21046327504504347 1106 50-51 0.1536389274406389 1106 52-53 0.17760408634890723 1106 54-55 0.3118529786271793 1106 56-57 0.4394632521793511 1106 58-59 0.4424604150945868 1106 60-61 0.3826865626426823 1106 62-63 0.5407514464282954 1106 64-65 0.28181716307583926 1106 66-67 0.4824375798221432 1106 68-69 0.6085538881090926 1106 70-71 0.2956137295883927 1106 72-73 0.46040404749217245 1106 74-75 0.5571055807175149 1106 76-77 0.41276094919299666 1106 78-79 0.5043203063610555 1106 80-81 0.6323765489106918 1106 82-83 0.5994565666767215 1106 84-85 0.736990119047821 1106 86-87 0.8091931430870609 1106 88-89 0.8701229276894331 1106 90-91 0.9450994291025161 1106 92-93 0.8317277820902795 1106 94-95 1.0393689566183753 1106 96-97 1.0082668042872562 1106 98-99 1.1299076164617752 1106 100-101 1.5175588763468397 1107 1 0.34518535571212183 1107 2 0.35308732200187265 1107 3 0.3493660053410146 1107 4 0.3419903716302741 1107 5 0.4392868223167383 1107 6 0.4868204801141971 1107 7 0.5935948215871107 1107 8 0.6198467769380969 1107 9 0.8153616848163807 1107 10-11 0.7198878949853764 1107 12-13 0.5352403790355638 1107 14-15 0.3210719010125871 1107 16-17 0.4334966949255019 1107 18-19 0.789961811964524 1107 20-21 0.5332308630698108 1107 22-23 0.57321544550733 1107 24-25 0.3760908600624404 1107 26-27 0.40905962026332077 1107 28-29 0.35763463596222067 1107 30-31 0.3352315863648485 1107 32-33 0.48236832832642307 1107 34-35 0.42429689691541483 1107 36-37 0.33616874803382046 1107 38-39 0.4424974342631387 1107 40-41 0.5671678356405252 1107 42-43 0.49763997248346215 1107 44-45 0.4912671996474103 1107 46-47 0.4888185885421805 1107 48-49 0.6589718877811492 1107 50-51 0.5447197075876034 1107 52-53 0.5289473904930233 1107 54-55 0.57190807890683 1107 56-57 0.8427810178000996 1107 58-59 0.8820264892101051 1107 60-61 0.7494354612632961 1107 62-63 0.7414787713123943 1107 64-65 0.8737924058206339 1107 66-67 0.7522762747822469 1107 68-69 0.7144799861039779 1107 70-71 0.8174234866139898 1107 72-73 0.9411140294156546 1107 74-75 0.869286774101667 1107 76-77 0.8685848561183143 1107 78-79 0.9755132790029535 1107 80-81 0.8764612753565046 1107 82-83 0.8316794356485104 1107 84-85 0.883640715048088 1107 86-87 1.1895283548435351 1107 88-89 1.2932597074690158 1107 90-91 1.1577431809550447 1107 92-93 1.319394181773955 1107 94-95 1.3370175183394544 1107 96-97 1.551603481368847 1107 98-99 1.7474623842551544 1107 100-101 1.932280622618375 1108 1 0.009255892038790847 1108 2 -0.04249076107370442 1108 3 0.10053938793547701 1108 4 0.19058488191365086 1108 5 0.10155165196870541 1108 6 -4.268464507788394E-4 1108 7 0.09169361652519115 1108 8 0.16072677007052505 1108 9 -0.28762508293896616 1108 10-11 -0.03694543790239635 1108 12-13 -0.008164009256425686 1108 14-15 0.40182462435033983 1108 16-17 0.43238101236749316 1108 18-19 0.40039685984292817 1108 20-21 0.4511918076453938 1108 22-23 0.34595928497023465 1108 24-25 0.27570556936861834 1108 26-27 0.12936860730019362 1108 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-6.261586511797848 2316 90-91 -5.4236164762767345 2316 92-93 -4.824637034617364 2316 94-95 -4.418318369810859 2316 96-97 -5.7865219695332755 2316 98-99 -6.660492020837275 2316 100-101 -7.3668440077107284 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5108.0 3 2021.0 4 2130.0 5 2279.0 6 2559.0 7 3146.0 8 3918.0 9 4994.0 10 6207.0 11 7889.0 12 9951.0 13 12381.0 14 13599.0 15 16455.0 16 20305.0 17 25089.0 18 19369.0 19 13427.0 20 13608.0 21 14111.0 22 15672.0 23 17541.0 24 19791.0 25 22709.0 26 26071.0 27 30665.0 28 36122.0 29 42683.0 30 51057.0 31 61135.0 32 74871.0 33 93548.0 34 121482.0 35 160435.0 36 212525.0 37 265127.0 38 203913.0 39 30065.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.30297042647325 18.916657505103238 14.61210930884175 21.168262759581758 2 15.58005083460103 20.723683233551075 40.4492072760364 23.247058655811497 3 19.382664942707255 26.760549996109805 29.684668659475054 24.172116401707882 4 12.412792804136766 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27.10081214102132 28.58574869473094 22.071013050315212 50-51 22.24926199820777 27.375071187257973 28.40108816293227 21.974578651601988 52-53 22.390856063766453 27.38979457616953 28.238301738612765 21.981047621451253 54-55 22.024559423001723 27.310176955283584 28.428673781062635 22.23658984065206 56-57 22.235524639312978 27.653171542685467 27.894663860094205 22.216639957907354 58-59 22.42951664612396 27.148974354330047 28.098056257221 22.32345274232499 60-61 22.154966736000688 27.30047167920469 28.562172723372097 21.98238886142252 62-63 22.24090228046494 27.287427898505324 28.504920984541343 21.96674883648839 64-65 22.035702981503597 27.450831349405476 28.383282391568866 22.13018327752206 66-67 22.010359555866334 27.616396543251664 28.047792321933073 22.325451578948933 68-69 22.130242628963444 27.453904297673006 28.2902128882681 22.125640185095445 70-71 22.20957315023653 27.398840086037445 28.11363142385793 22.277955339868093 72-73 22.19356685739775 27.50688327667431 27.985913598135436 22.313636267792504 74-75 21.99711045744161 28.073197785178994 27.74067766157903 22.189014095800363 76-77 22.081078295775196 27.72176338566436 27.877565530601935 22.31959278795851 78-79 22.15811138970896 27.774638066154107 27.827826727911336 22.2394238162256 80-81 22.06854015131763 28.12860988880132 27.63616537678256 22.16668458309849 82-83 22.173528189964728 28.053404344336457 27.545645582602702 22.227421883096117 84-85 22.01144075408614 27.980616339819793 27.538634443707068 22.469308462387 86-87 22.150043143149148 27.88692397363547 27.471556104800143 22.491476778415244 88-89 22.23340640663735 27.753484343576957 27.319142087936786 22.693967161848903 90-91 22.04343652187133 27.758254177382373 27.603134347016482 22.59517495372981 92-93 22.1697118711007 27.770690612574572 27.689504957551247 22.370092558773486 94-95 21.876086674413266 27.57850856080432 27.782814486359165 22.762590278423247 96-97 21.954821308359374 27.462932371529426 27.72861427009043 22.85363205002077 98-99 21.78421270993476 27.79304444049125 27.3536555993344 23.06908725023959 100-101 21.915785934981272 27.61104227868461 27.55467624275527 22.918495543578846 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 883.0 1 660.5 2 745.0 3 989.5 4 780.5 5 719.0 6 1112.0 7 1920.5 8 3108.5 9 3697.5 10 3169.5 11 2551.5 12 2511.5 13 2776.5 14 2901.5 15 3066.0 16 3274.5 17 3410.5 18 3629.0 19 3917.0 20 4273.5 21 4882.0 22 5565.5 23 6474.0 24 7612.0 25 9051.0 26 11157.5 27 13410.0 28 15474.0 29 18248.5 30 21827.0 31 25696.5 32 30387.5 33 33853.0 34 36400.0 35 38847.0 36 42424.0 37 46099.5 38 47135.0 39 47103.0 40 47585.5 41 50011.0 42 51454.0 43 52464.5 44 54594.5 45 57011.5 46 57651.0 47 58468.5 48 64404.0 49 64107.0 50 57485.0 51 53477.0 52 51546.5 53 51302.5 54 49975.5 55 46926.5 56 42991.0 57 39320.5 58 37425.5 59 34396.0 60 29458.0 61 25363.0 62 22006.0 63 17161.0 64 12344.0 65 9794.5 66 7548.5 67 5875.5 68 4463.5 69 3164.0 70 2530.0 71 2089.5 72 1791.5 73 1619.5 74 1310.5 75 1081.5 76 847.0 77 536.5 78 355.5 79 240.0 80 157.0 81 114.0 82 69.0 83 39.5 84 28.5 85 22.5 86 12.5 87 9.0 88 7.0 89 3.5 90 6.5 91 6.0 92 1.5 93 1.5 94 1.5 95 1.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07161686048548631 2 0.027435314713345503 3 0.01425211153940026 4 0.01941850197243285 5 0.023278448847687087 6 0.02963251524233637 7 0.07310145543750717 8 0.05219835851305345 9 0.06823198399487874 10-11 0.058047662624015645 12-13 0.03886669584390612 14-15 0.04412216197405997 16-17 0.05576138639790351 18-19 0.052762504594821374 20-21 0.018587128799301172 22-23 0.01202521911136897 24-25 0.021912621491827898 26-27 0.02298152985728292 28-29 0.006086839303285527 30-31 0.0013658273558591915 32-33 0.00902633730828683 34-35 0.010689083654550194 36-37 0.00424594156277966 38-39 0.0091451049044485 40-41 0.003859946875254237 42-43 0.001722130144344198 44-45 6.235298798487614E-4 46-47 7.126055769700129E-4 48-49 0.001009524567374185 50-51 0.0013064435577783572 52-53 2.0784329328292045E-4 54-55 2.6722709136375487E-4 56-57 0.0033551845915671444 58-59 0.004186557764698826 60-61 0.004958547139749673 62-63 0.0029691899040417205 64-65 7.126055769700129E-4 66-67 5.047622836870926E-4 68-69 0.0043350172599009125 70-71 0.0025535033174758797 72-73 0.020873405025413298 74-75 0.018022982717533245 76-77 0.0246739681025867 78-79 0.019685729063796606 80-81 0.012945667981621901 82-83 0.005374233726315515 84-85 0.004067790168537157 86-87 0.0019002815385867013 88-89 0.0021081248318696216 90-91 0.006027455505204693 92-93 0.009857710481418513 94-95 0.012559673294096478 96-97 0.01071877555359061 98-99 0.02212046478511082 100-101 0.019180966780109516 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1683961.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.9620168077915 #Duplication Level Percentage of deduplicated Percentage of total 1 77.61365687232463 42.6580311353087 2 12.755860939168134 14.021756866728206 3 4.177059712967262 6.887388784537663 4 1.8391012430612037 4.0432285372944055 5 0.9307413937032426 2.557771206221245 6 0.5582835813720479 1.841063494973072 7 0.3711631610655979 1.4279913127844295 8 0.27212488594239886 1.1965226043987578 9 0.19883288306587216 0.9835430634907297 >10 1.121903043730635 11.66945239734929 >50 0.10445100313804377 4.0008270492624085 >100 0.05235856330408518 5.310749790130824 >500 0.003265402797697233 1.2042048339833684 >1k 9.7962083930917E-4 1.0548831952719329 >5k 1.088467599232411E-4 0.4968414300875292 >10k+ 1.088467599232411E-4 0.6457442981775785 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10794 0.6409887164845266 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8305 0.4931824430613298 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4987 0.2961470010291212 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2243 0.1331978590953116 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2180 0.12945667981621903 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 2.9691899040417207E-5 18-19 0.0 0.0 0.0 2.9691899040417207E-5 5.9383798080834414E-5 20-21 0.0 0.0 0.0 2.6722709136375487E-4 5.9383798080834414E-5 22-23 0.0 0.0 0.0 4.453784856062581E-4 5.9383798080834414E-5 24-25 0.0 0.0 0.0 5.938379808083441E-4 5.9383798080834414E-5 26-27 0.0 0.0 0.0 8.313731731316818E-4 1.1876759616166883E-4 28-29 0.0 0.0 0.0 0.0011579840625762711 1.1876759616166883E-4 30-31 0.0 0.0 0.0 0.002612887115556714 1.1876759616166883E-4 32-33 0.0 0.0 0.0 0.0041865577646988264 1.1876759616166883E-4 34-35 0.0 0.0 0.0 0.005968071707123859 1.1876759616166883E-4 36-37 0.0 0.0 0.0 0.008788802115963494 1.1876759616166883E-4 38-39 0.0 0.0 0.0 0.013331662669147325 1.1876759616166883E-4 40-41 0.0 0.0 0.0 0.018052674616573665 1.1876759616166883E-4 42-43 0.0 0.0 0.0 0.023219065049606254 1.1876759616166883E-4 44-45 0.0 0.0 0.0 0.026514865843092567 1.1876759616166883E-4 46-47 0.0 0.0 0.0 0.03182971577132725 1.1876759616166883E-4 48-49 0.0 0.0 0.0 0.036788262911076924 1.1876759616166883E-4 50-51 0.0 0.0 0.0 0.041657734353705345 1.1876759616166883E-4 52-53 0.0 0.0 0.0 0.05273281269578096 1.1876759616166883E-4 54-55 0.0 0.0 0.0 0.0710824063027588 1.1876759616166883E-4 56-57 0.0 0.0 0.0 0.09789419113625553 1.4845949520208603E-4 58-59 0.0 0.0 0.0 0.13251494541738199 1.7815139424250324E-4 60-61 0.0 0.0 0.0 0.17482590154997651 1.7815139424250324E-4 62-63 0.0 0.0 0.0 0.20389427071054494 1.7815139424250324E-4 64-65 0.0 0.0 0.0 0.2357239864818722 1.7815139424250324E-4 66-67 0.0 0.0 0.0 0.2962954605243233 1.7815139424250324E-4 68-69 0.0 0.0 0.0 0.3775621881979452 1.7815139424250324E-4 70-71 0.0 0.0 0.0 0.4615011867852047 1.7815139424250324E-4 72-73 0.0 0.0 0.0 0.5536351495076193 1.7815139424250324E-4 74-75 0.0 0.0 0.0 0.6414340949701329 1.7815139424250324E-4 76-77 0.0 0.0 0.0 0.7241854175957757 1.7815139424250324E-4 78-79 0.0 0.0 0.0 0.8037300151250534 2.0784329328292045E-4 80-81 0.0 0.0 0.0 0.8860062673660494 2.3753519232333766E-4 82-83 0.0 0.0 0.0 1.0015374465323128 2.3753519232333766E-4 84-85 0.0 0.0 0.0 1.1814109709191603 2.3753519232333766E-4 86-87 0.0 0.0 0.0 1.413037475333455 2.6722709136375487E-4 88-89 0.0 0.0 0.0 1.6778298309758957 2.9691899040417205E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 15250 0.0 31.816422 1 GGTATCA 10090 0.0 31.744183 1 GTACATG 12490 0.0 26.90001 1 ATCAACG 18395 0.0 26.176306 3 TCAACGC 18415 0.0 26.122065 4 TACATGG 12775 0.0 26.00307 2 TATCAAC 18585 0.0 25.95445 2 CAACGCA 18610 0.0 25.722172 5 AACGCAG 19085 0.0 25.28124 6 ACATGGG 12860 0.0 24.395046 3 GAGTACT 9435 0.0 24.053513 12-13 TATCTCG 100 0.0016466446 23.768103 5 ACGCAGA 20920 0.0 22.972803 7 CGCAGAG 21100 0.0 22.733122 8 TATAGCG 105 0.0021869368 22.63629 5 GTACTTT 10115 0.0 22.27036 14-15 AGTACTT 9570 0.0 21.653175 12-13 GCAGAGT 21680 0.0 21.55419 9 CATGGGA 7835 0.0 21.173134 4 CATGGGG 4420 0.0 19.895195 4 >>END_MODULE