FastQCFastQC Report
Thu 26 May 2016
SRR936756_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936756_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1683961
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122670.7284610510575957No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106290.6311903898011889No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65780.3906266237757288No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29110.172866236213309No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT24630.14626229467309518No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20070.11918328274823466No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18640.11069139962267534No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107400.031.4053081
GTATCAA157000.030.8032911
ATCAACG197150.024.4272233
TCAACGC197900.024.3324814
TATCAAC199650.024.265522
CAACGCA200500.024.0406325
AACGCAG205600.023.5366866
GTACATG128200.023.1971871
TACATGG130200.022.5442712
TACCTGG12750.022.3511452
GAGTACT111750.022.16356812-13
AGTACTT113600.021.94895712-13
ACATGGG129300.021.4877173
ACGCAGA230600.020.9656587
CGCAGAG231450.020.888668
TACACCG1604.999859E-520.7764955
GTACTTT122200.020.15044814-15
GCAGAGT239750.019.947619
CATGGGG44800.019.5044674
GTATAGG5400.019.354321
Produced by FastQC (version 0.11.5)