##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936756_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1683961 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.730662408452453 31.0 31.0 34.0 30.0 34.0 2 31.867613917424453 31.0 31.0 34.0 30.0 34.0 3 31.931557203521933 31.0 31.0 34.0 30.0 34.0 4 35.68299800292287 37.0 35.0 37.0 33.0 37.0 5 35.50365774504279 37.0 35.0 37.0 33.0 37.0 6 35.60125798637854 37.0 35.0 37.0 33.0 37.0 7 35.54002081995961 37.0 35.0 37.0 33.0 37.0 8 35.50944232081385 37.0 35.0 37.0 33.0 37.0 9 37.08684583550332 39.0 37.0 39.0 33.0 39.0 10-11 36.976157108151554 39.0 37.0 39.0 33.0 39.0 12-13 36.860156203142466 39.0 37.0 39.0 33.0 39.0 14-15 37.89338321968265 40.0 37.0 41.0 33.0 41.0 16-17 37.801096640599155 39.0 37.0 41.0 32.5 41.0 18-19 37.998411780320325 40.0 37.0 41.0 33.0 41.0 20-21 38.03960661796799 40.0 37.0 41.0 33.0 41.0 22-23 37.93834447472358 40.0 37.0 41.0 33.0 41.0 24-25 37.790934588152574 40.0 37.0 41.0 32.0 41.0 26-27 37.563253246363786 39.0 36.5 41.0 32.0 41.0 28-29 37.44391497190256 39.0 36.0 41.0 32.0 41.0 30-31 37.33288211544092 39.0 36.0 41.0 32.0 41.0 32-33 37.10346617291018 39.0 35.5 41.0 31.0 41.0 34-35 36.886161259079046 39.0 35.0 41.0 31.0 41.0 36-37 36.62914847790418 39.0 35.0 41.0 30.0 41.0 38-39 36.42576965856098 39.0 35.0 40.0 30.0 41.0 40-41 36.17937559123994 39.0 35.0 40.0 30.0 41.0 42-43 35.82779292394539 38.0 35.0 40.0 29.0 41.0 44-45 35.443060735967165 38.0 35.0 40.0 27.5 41.0 46-47 34.94037391602299 38.0 34.0 40.0 25.5 41.0 48-49 34.44206694810628 38.0 34.0 40.0 24.0 41.0 50-51 34.10282750016182 38.0 33.0 40.0 23.0 41.0 52-53 33.728863376289596 38.0 33.0 40.0 21.0 41.0 54-55 33.510232125328315 37.0 33.0 40.0 20.5 41.0 56-57 33.201463394936106 37.0 32.5 40.0 19.5 41.0 58-59 32.824831157016106 36.5 32.0 39.5 18.0 41.0 60-61 32.4081873036252 36.0 31.0 39.0 14.5 40.5 62-63 32.07231937081678 35.5 31.0 39.0 13.0 40.0 64-65 31.64483441124824 35.0 31.0 38.5 10.5 40.0 66-67 31.220393465169323 35.0 30.0 38.0 8.0 40.0 68-69 30.867657267597053 35.0 30.0 38.0 7.5 40.0 70-71 30.50516549967606 34.5 29.5 37.0 6.5 39.0 72-73 30.054269665390116 34.0 29.0 36.5 2.0 39.0 74-75 29.55988648193159 34.0 29.0 36.0 2.0 39.0 76-77 28.10226276024207 32.0 26.5 34.5 2.0 36.5 78-79 28.860915425000933 33.5 28.0 35.0 2.0 37.0 80-81 28.65046280763034 34.0 28.0 35.0 2.0 37.0 82-83 28.34603503287784 33.5 27.5 35.0 2.0 36.5 84-85 27.95729414161017 33.0 27.0 35.0 2.0 36.0 86-87 27.558933668891385 33.0 26.0 35.0 2.0 36.0 88-89 27.069580293130304 33.0 25.5 35.0 2.0 35.0 90-91 26.575772835594172 32.0 25.0 34.5 2.0 35.0 92-93 25.954496273963592 32.0 24.0 34.0 2.0 35.0 94-95 25.38624825634323 32.0 21.5 34.0 2.0 35.0 96-97 24.632606990304406 31.0 18.0 34.0 2.0 35.0 98-99 23.724370695045785 31.0 2.0 34.0 2.0 35.0 100-101 20.57329415586228 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.33313482627013613 1101 2 0.33889729461687423 1101 3 0.2957758576983487 1101 4 0.31098229353420237 1101 5 0.25950700836034457 1101 6 0.2169792058502793 1101 7 0.38188887324032095 1101 8 0.3495621095903303 1101 9 0.359541123737273 1101 10-11 0.4460762309456996 1101 12-13 0.3784860719053569 1101 14-15 0.5069966359526035 1101 16-17 0.5268146060714329 1101 18-19 0.3890580859561297 1101 20-21 0.46306888018865067 1101 22-23 0.3346037430937727 1101 24-25 0.21654781918209665 1101 26-27 0.4960718026602464 1101 28-29 0.3770967453858205 1101 30-31 0.31737318304951145 1101 32-33 0.19532467790679675 1101 34-35 0.04213050261775919 1101 36-37 0.12434616122280318 1101 38-39 0.2848833032422817 1101 40-41 0.3241037860078748 1101 42-43 0.4513311338982291 1101 44-45 0.422768094510495 1101 46-47 0.5034126467085116 1101 48-49 0.3188508077551262 1101 50-51 0.4491665149670254 1101 52-53 0.4889239926377513 1101 54-55 0.26290775963545343 1101 56-57 0.2537236179960658 1101 58-59 0.06691631014972188 1101 60-61 0.48355472771421404 1101 62-63 0.39493984956908434 1101 64-65 0.5279995252455443 1101 66-67 0.23192731584739334 1101 68-69 0.14966783592799615 1101 70-71 0.011612076655513448 1101 72-73 -0.04824188723088341 1101 74-75 0.23823387418923758 1101 76-77 -0.026890470317823656 1101 78-79 0.2515347124467624 1101 80-81 0.36856254479350525 1101 82-83 0.3194701700780982 1101 84-85 0.30185230726664614 1101 86-87 0.412594300413776 1101 88-89 0.4101480297019613 1101 90-91 0.3759209864690227 1101 92-93 0.5957937291176911 1101 94-95 0.18894470468520908 1101 96-97 0.4375900988496433 1101 98-99 0.34147843202945793 1101 100-101 0.9302181099549252 1102 1 0.24936223938384217 1102 2 0.33311895672770575 1102 3 0.2777290686749545 1102 4 0.23697860153235695 1102 5 0.2767965820051259 1102 6 0.21420168446152132 1102 7 0.21631785545481108 1102 8 0.23745021353438744 1102 9 0.31777890285616195 1102 10-11 0.30363365222441274 1102 12-13 0.43383894208179186 1102 14-15 0.4704317951701853 1102 16-17 0.47460242246533824 1102 18-19 0.37545731915574976 1102 20-21 0.2764990694037408 1102 22-23 0.26021718340049205 1102 24-25 0.16799142740389783 1102 26-27 0.2535591114039022 1102 28-29 0.23551859209673864 1102 30-31 0.35307910090246963 1102 32-33 0.25005629777260197 1102 34-35 0.21252987781744537 1102 36-37 0.2906465268729832 1102 38-39 0.22728274944200422 1102 40-41 0.26151370471283286 1102 42-43 0.3227048070850671 1102 44-45 0.4441057351793134 1102 46-47 0.4798211099127485 1102 48-49 0.3731732899163731 1102 50-51 0.4071891639783516 1102 52-53 0.4303764758639943 1102 54-55 0.44344895740605494 1102 56-57 0.2708348040516597 1102 58-59 0.5430255082043232 1102 60-61 0.6368982968860024 1102 62-63 0.5124871533427324 1102 64-65 0.3160024717470229 1102 66-67 0.07918405197575851 1102 68-69 0.39072981895898806 1102 70-71 0.3980867473928491 1102 72-73 0.4916207277004254 1102 74-75 0.3854868828157443 1102 76-77 0.2189553176050687 1102 78-79 0.3871408752498411 1102 80-81 0.31050333076390046 1102 82-83 0.45717793393198036 1102 84-85 0.22312214290156263 1102 86-87 0.43379313601319325 1102 88-89 0.4858202225380559 1102 90-91 0.5723787946979293 1102 92-93 0.5820017947217231 1102 94-95 0.3363068758662955 1102 96-97 0.7839971420531633 1102 98-99 0.7389932532868677 1102 100-101 1.155752385222062 1103 1 0.15637992757800134 1103 2 0.1605137188283443 1103 3 0.20353737801267258 1103 4 0.19946591813732084 1103 5 0.2761918925769109 1103 6 0.20902960164237783 1103 7 0.16909311940654703 1103 8 0.13324206873800648 1103 9 0.2604362455135032 1103 10-11 0.21419583513954166 1103 12-13 0.24032529886227394 1103 14-15 0.3334189060984656 1103 16-17 0.37343760688876415 1103 18-19 0.33788910305812436 1103 20-21 0.25052141960256336 1103 22-23 0.10399752071024437 1103 24-25 0.2338107061643555 1103 26-27 0.18007837645257752 1103 28-29 0.1570570536351994 1103 30-31 0.21191977003725526 1103 32-33 0.3147354421576267 1103 34-35 0.23435765788052265 1103 36-37 0.2711305526511225 1103 38-39 0.15926890054580412 1103 40-41 0.238333943297782 1103 42-43 0.26543070863449714 1103 44-45 0.11852709813008033 1103 46-47 0.1771301733982824 1103 48-49 0.17258779697793614 1103 50-51 0.09209181035158309 1103 52-53 0.10670309042589565 1103 54-55 0.18856345075584358 1103 56-57 0.12689746687902925 1103 58-59 0.25107569331333224 1103 60-61 0.18368699838057978 1103 62-63 0.33499777997101177 1103 64-65 0.3691890890512859 1103 66-67 0.14179546017540545 1103 68-69 0.25324181676510094 1103 70-71 0.29093811171480155 1103 72-73 0.10104688977364518 1103 74-75 0.13854255263612103 1103 76-77 0.1862787784579396 1103 78-79 -0.08111018741298537 1103 80-81 -0.0745967652185513 1103 82-83 -0.01285908786974943 1103 84-85 0.37254624820802107 1103 86-87 0.3767110024604712 1103 88-89 0.31002905292335825 1103 90-91 0.38023358696448284 1103 92-93 0.4902165211717424 1103 94-95 0.13294543338720288 1103 96-97 0.4663905329759679 1103 98-99 0.40748152971632123 1103 100-101 0.7182403874159498 1104 1 0.30120738487028476 1104 2 0.39819790670949473 1104 3 0.3244687820054146 1104 4 0.34662175423519415 1104 5 0.39219828348481656 1104 6 0.29694656181595036 1104 7 0.33397054174035645 1104 8 0.3720087887934156 1104 9 0.4647682106783364 1104 10-11 0.38464808500933856 1104 12-13 0.4040188553526747 1104 14-15 0.5061560826341847 1104 16-17 0.5884315793391224 1104 18-19 0.4608729237768827 1104 20-21 0.41271956660656883 1104 22-23 0.4620807008952781 1104 24-25 0.47021543349480055 1104 26-27 0.3699893143235542 1104 28-29 0.5167763298570094 1104 30-31 0.6048318898064409 1104 32-33 0.49540255220009044 1104 34-35 0.48634629014490116 1104 36-37 0.4089750051410519 1104 38-39 0.46017857518929617 1104 40-41 0.59037596430435 1104 42-43 0.721982340270543 1104 44-45 0.7039236702317808 1104 46-47 0.5891113876445502 1104 48-49 0.70572948177972 1104 50-51 0.653673986382266 1104 52-53 0.7791872607294295 1104 54-55 0.7377465177357578 1104 56-57 0.4747871030354105 1104 58-59 0.648367624792094 1104 60-61 0.6951877139471421 1104 62-63 0.6342856239971724 1104 64-65 0.6926433734650566 1104 66-67 0.6988545144207308 1104 68-69 0.7948320732540637 1104 70-71 0.7680127628187172 1104 72-73 0.9606766381906731 1104 74-75 0.6660598908597031 1104 76-77 0.7533314968893876 1104 78-79 0.8461501109080913 1104 80-81 1.1817559376029223 1104 82-83 1.0612532095121026 1104 84-85 1.4108228281278201 1104 86-87 1.3444439354438273 1104 88-89 1.2179129763531442 1104 90-91 1.1952322489665228 1104 92-93 1.1751794901105157 1104 94-95 1.2132394125275532 1104 96-97 1.3623069172857107 1104 98-99 1.2634794991076497 1104 100-101 1.9156062367232707 1105 1 -8.618502520114646E-4 1105 2 0.09000971303022354 1105 3 0.04209881657411074 1105 4 0.08608224298893674 1105 5 0.22808509741128802 1105 6 0.11313725869121072 1105 7 0.06900693108506317 1105 8 0.15352864490693463 1105 9 0.08752680201583019 1105 10-11 0.25922188751852815 1105 12-13 0.2034327796168114 1105 14-15 0.24616008648671794 1105 16-17 0.12206740845974196 1105 18-19 0.19495311747507316 1105 20-21 0.2134878649219587 1105 22-23 0.1572339901231814 1105 24-25 0.1727673377680432 1105 26-27 0.1583770385747698 1105 28-29 0.07640094503791772 1105 30-31 0.12366733619658277 1105 32-33 0.021610919621345204 1105 34-35 0.17292203468019807 1105 36-37 0.1794560506825107 1105 38-39 0.17784297353163936 1105 40-41 0.09848849462880338 1105 42-43 0.05204654377974549 1105 44-45 0.07861553910088048 1105 46-47 0.00909281859621558 1105 48-49 0.2599239901964765 1105 50-51 0.3205785197206481 1105 52-53 0.23969020135418617 1105 54-55 -0.047713507579942416 1105 56-57 0.13984040629255645 1105 58-59 0.05798349139760006 1105 60-61 0.15383106999524188 1105 62-63 0.12377566874890533 1105 64-65 0.14761311600472382 1105 66-67 -0.10505964550323199 1105 68-69 0.08659816629792161 1105 70-71 0.12423520677660349 1105 72-73 0.25697086775644706 1105 74-75 0.21150929177933264 1105 76-77 0.09405335682075844 1105 78-79 0.25109045508177275 1105 80-81 0.35002714028770754 1105 82-83 0.49031518883393943 1105 84-85 0.5540745238539451 1105 86-87 0.6143253488983476 1105 88-89 0.3568846483083661 1105 90-91 0.5086252932973672 1105 92-93 0.50179171068811 1105 94-95 0.569962338051166 1105 96-97 0.4620923801484018 1105 98-99 0.28819493395913653 1105 100-101 1.060024093905536 1106 1 0.10164858788550646 1106 2 0.20196468369773513 1106 3 0.10119188776485188 1106 4 0.14063009986754338 1106 5 0.11267893494630243 1106 6 0.11038370443932166 1106 7 0.08272185101530027 1106 8 0.055785396664241205 1106 9 0.014338281324533853 1106 10-11 0.1343606222577094 1106 12-13 0.2009699076755851 1106 14-15 0.22138074078616654 1106 16-17 0.08579554122228217 1106 18-19 0.059397385748418685 1106 20-21 -0.05112024247194569 1106 22-23 -0.054205680084521646 1106 24-25 -0.01595418857834119 1106 26-27 -0.09149083310220618 1106 28-29 -0.16699528137717579 1106 30-31 -0.07805297456922489 1106 32-33 0.05436817346627265 1106 34-35 0.07221911200169018 1106 36-37 -0.3124999770115906 1106 38-39 0.07711626775498104 1106 40-41 0.1773294649792021 1106 42-43 0.15203333094743954 1106 44-45 -0.008435885242441543 1106 46-47 0.13875562711584877 1106 48-49 -0.16050040597708204 1106 50-51 -0.09465323335794551 1106 52-53 -0.3201507161448873 1106 54-55 -0.25137346212779477 1106 56-57 -0.31339938018807345 1106 58-59 -0.37687460278346663 1106 60-61 -0.43415053774219103 1106 62-63 -0.3751488067016595 1106 64-65 -0.35396978130514256 1106 66-67 -0.1695673353716316 1106 68-69 -0.33543555285011095 1106 70-71 -0.14219627147507552 1106 72-73 -0.07503853978015229 1106 74-75 -0.18457970985906158 1106 76-77 -0.29427442712301044 1106 78-79 -0.32602227680343177 1106 80-81 -0.16715482683876814 1106 82-83 -0.18778322034105344 1106 84-85 0.03610771448868988 1106 86-87 0.05687859661252759 1106 88-89 0.17817316371452918 1106 90-91 0.04232330080225566 1106 92-93 0.04109724422227856 1106 94-95 -0.16569645376744901 1106 96-97 0.16384175914528143 1106 98-99 0.12217305350884189 1106 100-101 0.6735936726804432 1107 1 0.0824902224416526 1107 2 0.08104733173165357 1107 3 0.13085294262001312 1107 4 0.16303132460976855 1107 5 0.19182676817954558 1107 6 0.009710740313849442 1107 7 0.03408850374636785 1107 8 0.06713823705115374 1107 9 0.018799859331174673 1107 10-11 0.03333131635185538 1107 12-13 0.12353281318208786 1107 14-15 0.15555159161106502 1107 16-17 0.13298801528387827 1107 18-19 0.0307267821263153 1107 20-21 -0.045958654147796096 1107 22-23 0.0673523416013424 1107 24-25 0.2714400792988343 1107 26-27 0.12797261458056397 1107 28-29 0.27417254158626037 1107 30-31 0.2684661477147472 1107 32-33 0.14449461366854877 1107 34-35 0.37264177211842764 1107 36-37 0.26603626041282524 1107 38-39 0.1293483688117263 1107 40-41 0.11982201460315167 1107 42-43 -0.024133546741147427 1107 44-45 0.06403240332104332 1107 46-47 0.04128869533487034 1107 48-49 0.13815321848102968 1107 50-51 0.13874019599781917 1107 52-53 0.20663291610800627 1107 54-55 0.09831367277097769 1107 56-57 -0.022788643177847234 1107 58-59 0.17025122492840694 1107 60-61 0.43665633336227927 1107 62-63 0.44138780763992 1107 64-65 0.13421801051491755 1107 66-67 0.36993933850735417 1107 68-69 0.38614104631935575 1107 70-71 0.39885594169066607 1107 72-73 0.41704127958587733 1107 74-75 0.3518929895894587 1107 76-77 0.3194139578048336 1107 78-79 0.3286027711312478 1107 80-81 0.56854960569515 1107 82-83 0.5739851533269906 1107 84-85 0.6540495380401694 1107 86-87 0.7728439309684667 1107 88-89 0.6832147289703983 1107 90-91 0.721841133099165 1107 92-93 1.0059315288937363 1107 94-95 1.125184297872174 1107 96-97 1.2861269267988753 1107 98-99 1.1115337912645522 1107 100-101 1.6509644786569666 1108 1 -0.018181414893788883 1108 2 0.019832122922871065 1108 3 -0.13220427116181455 1108 4 -0.021124022601874515 1108 5 -0.0615154525931203 1108 6 0.009274685469556232 1108 7 0.06650775562715694 1108 8 -0.0320532495423862 1108 9 -0.03744497774085431 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5 89.0 6 197.0 7 481.0 8 1125.0 9 1984.0 10 3318.0 11 5044.0 12 7136.0 13 9544.0 14 12392.0 15 16222.0 16 20616.0 17 25915.0 18 22500.0 19 14107.0 20 12501.0 21 12692.0 22 13486.0 23 14955.0 24 17425.0 25 21128.0 26 25584.0 27 31277.0 28 38301.0 29 46445.0 30 56488.0 31 67981.0 32 83406.0 33 103069.0 34 132370.0 35 173136.0 36 232063.0 37 286330.0 38 165660.0 39 8935.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.556519626681144 19.939225505330537 15.51204828222364 20.99220658576468 2 15.91661059187071 21.579046457250815 39.293297215819564 23.211045735058914 3 19.267300025477677 26.69276594329465 30.419277039743392 23.620656991484278 4 12.818408502334675 17.89316973492854 37.15192929052395 32.136492472212836 5 12.891708790999962 38.69341720193075 33.31110324579073 15.103770761278554 6 29.77533327672078 37.22740609788469 17.67618133674117 15.321079288653362 7 27.419518623056 34.58233296376817 20.384379448217622 17.613768964958215 8 27.180973906165285 33.54418540571902 19.562270147586553 19.712570540529146 9 27.357284402667283 16.495156360509537 20.535570598131432 35.61198863869175 10-11 24.914413101016 27.324742081318988 26.32082928286344 21.440015534801578 12-13 26.72495062916453 25.05571013685468 27.405557028622468 20.81378220535833 14-15 23.415607752687418 25.669048752997405 27.127309588481424 23.788033905833757 16-17 21.115400272156027 29.192311814914902 27.595273167036876 22.0970147458922 18-19 21.006563187005696 26.582005988343987 31.671177917476772 20.74025290717354 20-21 22.561834864346622 25.301743923998238 31.610708324005127 20.52571288765001 22-23 21.899107994909613 25.46956309506348 31.654897773639384 20.976431136387525 24-25 21.58749519733533 25.719627711093075 31.938447505613254 20.754429585958345 26-27 21.364641718972184 25.87398634093388 31.822815171474694 20.938556768619243 28-29 21.03464555706659 26.047799466717343 31.959635609979152 20.95791936623691 30-31 21.15010663981693 25.83620135996572 31.874541811032998 21.13915018918435 32-33 21.026877223176896 25.91456289807097 31.656346404477663 21.402213474274472 34-35 21.68027473875055 25.848715532205375 31.500593553045945 20.97041617599813 36-37 21.84677107826297 25.532848618229405 31.396640447473178 21.22373985603445 38-39 21.552693745256654 25.788657302109698 31.384973793571973 21.273675159061675 40-41 21.53664165020743 25.899666143692418 31.382849215954316 21.180842990145834 42-43 21.56432885285263 26.096654738711873 30.736620246522943 21.60239616191256 44-45 21.61272267577635 26.17599681695565 30.522515139503152 21.688765367764848 46-47 22.097476360738426 26.538652709065612 29.76614010927833 21.597730820917636 48-49 22.283318561538998 26.79689599320646 29.068278351081755 21.85150709417279 50-51 22.21620135419054 27.03942141074777 28.915923081216988 21.828454153844692 52-53 22.521678842844906 27.051751602994734 28.647575725634404 21.77899382852596 54-55 22.028808298709354 27.06080349801906 28.76931240088258 22.141075802389 56-57 22.29670596291955 27.370997827059863 28.27821592616339 22.054080283857196 58-59 22.492594046843283 26.85252782541494 28.445631957838785 22.20924616990299 60-61 22.235253482430224 27.016732712287695 28.9312488992809 21.816764906001186 62-63 22.279540246815618 26.944602497140203 28.928761063523723 21.847096192520457 64-65 22.13883577003594 27.073102860043115 28.748507285161175 22.03955408475977 66-67 22.0852782231724 27.254528178632935 28.416978442900408 22.24321515529426 68-69 22.218283052504184 27.0898878940011 28.58996925072241 22.101859802772307 70-71 22.290223390369594 27.087670832645472 28.50258141830141 22.119524358683528 72-73 22.26004640249982 27.15929881986578 28.348934446819136 22.231720330815264 74-75 22.113405781071798 27.708632176048063 28.042940116448072 22.135021926432064 76-77 22.147986993378215 27.3819615817102 28.270577165130117 22.199474259781468 78-79 22.167619484278806 27.410442469256637 28.16045226864824 22.261485777816315 80-81 22.163147158284183 27.72541271453895 27.969318847651177 22.142121279525696 82-83 22.201334683994812 27.680363645111022 27.950941409267354 22.16736026162681 84-85 22.041610629814613 27.62098019740813 27.86676020662748 22.47064896614978 86-87 22.170736784527207 27.463577698781087 27.75388113060358 22.61180438608812 88-89 22.242088553072314 27.438370146281414 27.60573053373527 22.713810766911006 90-91 22.012343386781733 27.402853387004537 27.912838756154983 22.671964470058747 92-93 22.12864238145052 27.365925492942605 27.98995610697566 22.51547601863122 94-95 21.823161507761007 27.26131627166359 28.030006234467663 22.88551598610774 96-97 21.974409608473366 27.139011577143275 27.97388257795759 22.91269623642577 98-99 21.7263578583722 27.355033873484917 27.641256873704474 23.27735139443841 100-101 21.67753525010221 27.34833304809987 27.832546564362932 23.14158513743499 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1062.0 1 873.0 2 1190.0 3 1422.5 4 896.5 5 674.0 6 1072.0 7 2154.5 8 3407.0 9 4030.5 10 3471.5 11 2664.5 12 2580.0 13 2757.5 14 2919.5 15 3151.0 16 3447.5 17 3747.5 18 4030.5 19 4238.0 20 4559.5 21 5202.5 22 5975.0 23 6913.5 24 7940.5 25 9257.5 26 11391.0 27 13910.0 28 15934.0 29 18556.0 30 22133.0 31 26022.0 32 30823.0 33 34435.5 34 36925.0 35 38958.5 36 42135.0 37 45779.0 38 46720.5 39 46980.0 40 47708.5 41 49758.5 42 51198.5 43 52429.0 44 54550.5 45 56830.5 46 57390.5 47 58506.5 48 65193.5 49 64471.0 50 56766.5 51 52824.0 52 50661.5 53 50452.5 54 49704.5 55 46330.0 56 42305.0 57 38771.0 58 36869.5 59 34089.5 60 29267.5 61 24980.0 62 21550.0 63 16771.5 64 12085.5 65 9667.5 66 7536.5 67 5733.0 68 4282.0 69 3113.0 70 2373.5 71 1857.0 72 1616.0 73 1564.0 74 1318.0 75 1073.5 76 790.5 77 512.5 78 368.0 79 270.0 80 207.0 81 148.0 82 103.5 83 71.5 84 34.5 85 20.5 86 12.0 87 5.0 88 4.0 89 2.0 90 1.5 91 1.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021378167309100388 2 0.011757992020005213 3 0.007957428942831812 4 0.0 5 0.0033848764906075617 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0012767516587379399 14-15 1.7815139424250324E-4 16-17 2.9691899040417207E-5 18-19 0.0013658273558591915 20-21 0.0 22-23 1.1876759616166883E-4 24-25 0.0 26-27 0.001454903052980443 28-29 0.003028573702122555 30-31 0.0012767516587379399 32-33 0.008165272236114731 34-35 0.00201904913474837 36-37 9.798326683337678E-4 38-39 0.0013658273558591915 40-41 1.7815139424250324E-4 42-43 0.006027455505204693 44-45 0.002286276226112125 46-47 2.3753519232333766E-4 48-49 5.047622836870926E-4 50-51 1.7815139424250324E-4 52-53 2.0784329328292045E-4 54-55 0.0021675086299504562 56-57 0.01766667992904824 58-59 0.030760807405872226 60-61 0.023901978727535852 62-63 0.016330544472229463 64-65 0.021823545794706647 66-67 0.0036224116829308996 68-69 0.0011282921635358538 70-71 1.7815139424250324E-4 72-73 0.0 74-75 0.0020487410337887875 76-77 0.002939498005001303 78-79 0.010867235048792698 80-81 0.019091891082988265 82-83 0.020279567044604953 84-85 0.03236416995405476 86-87 0.012203370505611471 88-89 0.045547373127999995 90-91 0.051871747623608855 92-93 0.032661088944458926 94-95 0.034294143391681874 96-97 0.05620676488350977 98-99 0.05982917656644067 100-101 0.06704430803326206 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1683961.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.104036004383346 #Duplication Level Percentage of deduplicated Percentage of total 1 75.68719972529875 39.436085795579196 2 13.845375974317777 14.427999365201554 3 4.496535708539592 7.028629753582269 4 1.9494604956851957 4.062990394252178 5 1.0408573758379545 2.7116435093044373 6 0.6208659538945627 1.9409773209370862 7 0.42502823536604795 1.5501980534874453 8 0.29439620992965587 1.2271384577383029 9 0.21577675289914133 1.011855573176921 >10 1.2406898478440267 12.278037673401759 >50 0.1158159570920786 4.190663823534376 >100 0.061598312271482165 5.703828555629496 >500 0.0049138641789663 1.6658166584306828 >1k 0.0011427591113875115 1.0091834915731366 >5k 1.1427591113875116E-4 0.3916696564734258 >10k+ 2.2855182227750231E-4 1.363281917697713 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12267 0.7284610510575957 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10629 0.6311903898011889 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6578 0.3906266237757288 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2911 0.172866236213309 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2463 0.14626229467309518 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2007 0.11918328274823466 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1864 0.11069139962267534 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 1.1876759616166883E-4 14-15 0.0 0.0 0.0 0.0 1.1876759616166883E-4 16-17 0.0 0.0 0.0 0.0 1.1876759616166883E-4 18-19 0.0 0.0 0.0 2.9691899040417207E-5 1.1876759616166883E-4 20-21 0.0 0.0 0.0 2.0784329328292045E-4 1.4845949520208603E-4 22-23 0.0 0.0 0.0 3.859946875254237E-4 1.7815139424250324E-4 24-25 0.0 0.0 0.0 5.344541827275097E-4 1.7815139424250324E-4 26-27 0.0 0.0 0.0 7.422974760104301E-4 1.7815139424250324E-4 28-29 0.0 0.0 0.0 0.0010986002644954368 1.7815139424250324E-4 30-31 0.0 0.0 0.0 0.0025535033174758797 1.7815139424250324E-4 32-33 0.0 0.0 0.0 0.0041865577646988264 1.7815139424250324E-4 34-35 0.0 0.0 0.0 0.00605714740424511 1.7815139424250324E-4 36-37 0.0 0.0 0.0 0.00923418060156975 1.7815139424250324E-4 38-39 0.0 0.0 0.0 0.014103652044198174 1.7815139424250324E-4 40-41 0.0 0.0 0.0 0.018943431587786178 1.7815139424250324E-4 42-43 0.0 0.0 0.0 0.024377049112182525 1.7815139424250324E-4 44-45 0.0 0.0 0.0 0.027880693198951756 1.7815139424250324E-4 46-47 0.0 0.0 0.0 0.033195543127186436 1.7815139424250324E-4 48-49 0.0 0.0 0.0 0.038599468752542374 1.7815139424250324E-4 50-51 0.0 0.0 0.0 0.04361739969037288 1.7815139424250324E-4 52-53 0.0 0.0 0.0 0.054722169931488915 1.7815139424250324E-4 54-55 0.0 0.0 0.0 0.073517142024073 1.7815139424250324E-4 56-57 0.0 0.0 0.0 0.10053677015085266 1.7815139424250324E-4 58-59 0.0 0.0 0.0 0.1350981406338983 1.7815139424250324E-4 60-61 0.0 0.0 0.0 0.17723094537225031 1.7815139424250324E-4 62-63 0.0 0.0 0.0 0.20615085503761665 1.7815139424250324E-4 64-65 0.0 0.0 0.0 0.2379508789099035 1.7815139424250324E-4 66-67 0.0 0.0 0.0 0.29864112054851627 1.7815139424250324E-4 68-69 0.0 0.0 0.0 0.3800859996163807 1.7815139424250324E-4 70-71 0.0 0.0 0.0 0.46497513897293347 1.7815139424250324E-4 72-73 0.0 0.0 0.0 0.5569012584020652 2.0784329328292045E-4 74-75 0.0 0.0 0.0 0.6439579063885683 2.3753519232333766E-4 76-77 0.0 0.0 0.0 0.7267389209132515 2.3753519232333766E-4 78-79 0.0 0.0 0.0 0.8064022860386909 2.3753519232333766E-4 80-81 0.0 0.0 0.0 0.8891536086643337 2.3753519232333766E-4 82-83 0.0 0.0 0.0 1.0045660202344353 2.3753519232333766E-4 84-85 0.0 0.0 0.0 1.1836081714481512 2.3753519232333766E-4 86-87 0.0 0.0 0.0 1.4148189892758798 2.3753519232333766E-4 88-89 0.0 0.0 0.0 1.6788393555432697 2.3753519232333766E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10740 0.0 31.405308 1 GTATCAA 15700 0.0 30.803291 1 ATCAACG 19715 0.0 24.427223 3 TCAACGC 19790 0.0 24.332481 4 TATCAAC 19965 0.0 24.26552 2 CAACGCA 20050 0.0 24.040632 5 AACGCAG 20560 0.0 23.536686 6 GTACATG 12820 0.0 23.197187 1 TACATGG 13020 0.0 22.544271 2 TACCTGG 1275 0.0 22.351145 2 GAGTACT 11175 0.0 22.163568 12-13 AGTACTT 11360 0.0 21.948957 12-13 ACATGGG 12930 0.0 21.487717 3 ACGCAGA 23060 0.0 20.965658 7 CGCAGAG 23145 0.0 20.88866 8 TACACCG 160 4.999859E-5 20.776495 5 GTACTTT 12220 0.0 20.150448 14-15 GCAGAGT 23975 0.0 19.94761 9 CATGGGG 4480 0.0 19.504467 4 GTATAGG 540 0.0 19.35432 1 >>END_MODULE