FastQCFastQC Report
Thu 26 May 2016
SRR936751_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936751_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences985306
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54120.5492709878961459No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41240.41855017629041125No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25490.2587013577507901No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11140.11306132308135745No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10180.10331815699894245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41750.037.2061581
GTATCAA65200.034.7531621
ATCAACG78900.028.5366423
TATCAAC79650.028.2097282
TCAACGC79750.028.1729244
CAACGCA81050.027.7796525
AACGCAG82100.027.4836246
GTACATG51850.024.9197521
GAGTACT49550.024.73304212-13
ACGCAGA90500.024.72277
CGCAGAG90900.024.561658
TACATGG51950.024.0488192
ACATGGG52050.023.2713153
GCAGAGT94650.022.9349129
GTACTTT53650.022.71183414-15
AGTACTT51650.022.53187212-13
TAGCCCT6100.021.8036754
AGAGTAC78050.021.48341410-11
GTCCTAC1553.9036167E-521.4530931
CATGGGG22550.020.643394
Produced by FastQC (version 0.11.5)