##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936751_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 985306 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.4597992907787 31.0 31.0 34.0 30.0 34.0 2 31.51512322060355 31.0 31.0 34.0 30.0 34.0 3 31.52217077740316 33.0 31.0 34.0 28.0 34.0 4 35.18055304646475 37.0 35.0 37.0 33.0 37.0 5 35.190187616841875 37.0 35.0 37.0 33.0 37.0 6 35.1838332457125 37.0 35.0 37.0 32.0 37.0 7 35.11415742926563 37.0 35.0 37.0 32.0 37.0 8 35.08778288166316 37.0 35.0 37.0 32.0 37.0 9 36.59114630378786 39.0 37.0 39.0 32.0 39.0 10-11 36.47743797358385 39.0 37.0 39.0 32.0 39.0 12-13 36.36891838677528 39.0 37.0 39.0 32.0 39.0 14-15 37.44438428264925 40.0 37.0 41.0 32.0 41.0 16-17 37.36169575746012 40.0 37.0 41.0 32.0 41.0 18-19 37.355275416977065 40.0 37.0 41.0 32.0 41.0 20-21 37.23921604049909 39.5 37.0 41.0 31.0 41.0 22-23 37.32459510040536 40.0 37.0 41.0 31.5 41.0 24-25 37.3513964189805 40.0 37.0 41.0 31.5 41.0 26-27 37.25495835811412 40.0 37.0 41.0 31.0 41.0 28-29 37.10129797240654 39.0 36.0 41.0 31.0 41.0 30-31 36.869092444377685 39.0 36.0 41.0 30.5 41.0 32-33 36.619880524425916 39.0 35.0 41.0 30.0 41.0 34-35 36.402761172671234 39.0 35.0 40.5 30.0 41.0 36-37 36.18868554540417 39.0 35.0 40.0 30.0 41.0 38-39 35.97587297753185 38.0 35.0 40.0 29.5 41.0 40-41 35.73458955897965 38.0 35.0 40.0 28.5 41.0 42-43 35.4616712980536 38.0 35.0 40.0 27.0 41.0 44-45 35.130489918867845 38.0 34.5 40.0 26.0 41.0 46-47 34.877754220516266 38.0 34.0 40.0 24.5 41.0 48-49 34.677170340990514 38.0 34.0 40.0 24.0 41.0 50-51 33.6847456526196 37.5 33.0 39.5 22.5 40.5 52-53 33.798157120732036 37.5 33.0 39.5 23.0 40.5 54-55 34.22929475716173 38.0 34.0 40.0 23.0 41.0 56-57 34.130229086192514 38.0 34.0 40.0 22.5 41.0 58-59 33.90277284417227 38.0 33.5 40.0 22.0 41.0 60-61 33.62851642027959 37.5 33.0 40.0 20.5 41.0 62-63 33.2626163851636 37.0 33.0 40.0 19.0 41.0 64-65 32.899447988746644 36.5 32.0 39.5 18.0 41.0 66-67 32.44072298351984 36.0 32.0 39.0 13.5 41.0 68-69 32.06472659255094 35.5 31.0 39.0 12.0 40.5 70-71 31.677885347293124 35.0 31.0 38.5 8.0 40.0 72-73 31.284935847340826 35.0 31.0 37.5 7.5 40.0 74-75 30.85822881419579 35.0 30.5 37.0 6.0 39.0 76-77 30.385257980769424 35.0 30.0 36.5 2.0 39.0 78-79 29.899114082325696 34.0 29.5 36.0 2.0 39.0 80-81 29.457871463281457 34.0 29.0 35.5 2.0 37.5 82-83 29.046425171469572 34.0 29.0 35.0 2.0 37.0 84-85 28.68117214347624 34.0 29.0 35.0 2.0 36.5 86-87 28.321133739163265 34.0 28.0 35.0 2.0 36.0 88-89 27.910013234467264 34.0 27.0 35.0 2.0 36.0 90-91 27.501389923536443 34.0 26.0 35.0 2.0 35.5 92-93 27.03433400385261 33.0 25.5 35.0 2.0 35.0 94-95 26.51035515870197 33.0 25.0 35.0 2.0 35.0 96-97 25.920551077533275 33.0 23.5 35.0 2.0 35.0 98-99 25.184258494315472 32.5 19.0 35.0 2.0 35.0 100-101 22.393867996338194 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3360807381982376 1101 2 0.2254140539197209 1101 3 0.19924965005690254 1101 4 0.1795711913459499 1101 5 0.24011239225703918 1101 6 0.29294907764846556 1101 7 0.36464582807289503 1101 8 0.45372002667421185 1101 9 0.4943873062305997 1101 10-11 0.559414960582977 1101 12-13 0.4780260723793148 1101 14-15 0.5614715775785655 1101 16-17 0.6621767434804724 1101 18-19 0.5162510538816392 1101 20-21 0.6101198600146276 1101 22-23 0.37306545934364976 1101 24-25 0.37977867944162114 1101 26-27 0.3782911757204701 1101 28-29 0.29121464823415977 1101 30-31 0.33390460899617835 1101 32-33 0.18287781636475842 1101 34-35 -0.09348113497743071 1101 36-37 -0.1661019080528945 1101 38-39 -0.072464933022232 1101 40-41 -0.24221794123400997 1101 42-43 -0.13800282318124601 1101 44-45 -0.23280287885012996 1101 46-47 0.16071628015544803 1101 48-49 0.35291451080760794 1101 50-51 0.18311132112287964 1101 52-53 0.2811743500724191 1101 54-55 0.1290801670366264 1101 56-57 0.11167687000542514 1101 58-59 0.21615660551653804 1101 60-61 0.18993939347473088 1101 62-63 0.2784616919748615 1101 64-65 0.029006037682179908 1101 66-67 0.1519092156381916 1101 68-69 0.23127649639552672 1101 70-71 0.23625810870171549 1101 72-73 0.09799315179934709 1101 74-75 0.06405641698680853 1101 76-77 0.14034564672196126 1101 78-79 0.10964136085878096 1101 80-81 0.25615372741785336 1101 82-83 0.07994275995078937 1101 84-85 -0.0187275820652566 1101 86-87 0.04291527412448559 1101 88-89 0.15042858205075404 1101 90-91 0.14059282828810638 1101 92-93 -0.007147032442343715 1101 94-95 -0.07600988307662959 1101 96-97 -0.17207517804179417 1101 98-99 -0.0950188675501451 1101 100-101 0.14071628126803049 1102 1 0.31645379651247296 1102 2 0.39159052450795784 1102 3 0.3613135665325835 1102 4 0.4222182501694789 1102 5 0.3797164156845909 1102 6 0.4015115935639244 1102 7 0.4519771242277244 1102 8 0.4177956569263088 1102 9 0.3610985361771242 1102 10-11 0.5359205628238755 1102 12-13 0.4961537780070131 1102 14-15 0.40244433031602256 1102 16-17 0.32569533273435525 1102 18-19 0.30008413256766175 1102 20-21 0.26016442329449063 1102 22-23 0.2462192663209848 1102 24-25 0.22159495140494556 1102 26-27 0.05077653349994904 1102 28-29 0.05217721507907669 1102 30-31 0.23816040222256163 1102 32-33 0.2241115825985176 1102 34-35 0.3090430565173463 1102 36-37 0.03717349301662409 1102 38-39 0.10122045022197312 1102 40-41 0.04231860065547011 1102 42-43 -0.10472996858486283 1102 44-45 -0.1406396499221998 1102 46-47 -0.11296591999733607 1102 48-49 0.15660689685293505 1102 50-51 0.11919484544169734 1102 52-53 0.14832166354325693 1102 54-55 0.1003432177114405 1102 56-57 0.2505806133209276 1102 58-59 0.20937025461254422 1102 60-61 0.015642856678191208 1102 62-63 0.1923781676560452 1102 64-65 0.287781820722671 1102 66-67 0.4049904481311941 1102 68-69 0.327033435534819 1102 70-71 0.20171838372081297 1102 72-73 0.04027097201834451 1102 74-75 -0.012678367836208793 1102 76-77 0.2977049540812686 1102 78-79 0.24636150346128005 1102 80-81 0.44606969380441086 1102 82-83 0.44067296112216425 1102 84-85 0.0086820181384617 1102 86-87 0.01615184147869897 1102 88-89 -0.08768575459299299 1102 90-91 -0.2738233927457614 1102 92-93 -0.17549118828649668 1102 94-95 -0.41923626453830565 1102 96-97 -0.18037986355236058 1102 98-99 -0.46392961366677454 1102 100-101 -0.3928462346474255 1103 1 0.09920892841597961 1103 2 0.10127403970508198 1103 3 0.18619253304456151 1103 4 0.3109393505499298 1103 5 0.24053333946902455 1103 6 0.20508030463043525 1103 7 0.19560854694606178 1103 8 0.15211732589240512 1103 9 -0.06262988056271013 1103 10-11 0.2311118333619433 1103 12-13 -0.080363634928311 1103 14-15 0.39657786432664466 1103 16-17 0.3396215002162677 1103 18-19 0.3630984726320463 1103 20-21 0.2530244926953813 1103 22-23 0.2734185639504858 1103 24-25 0.20836028440381682 1103 26-27 -0.0015918765506413024 1103 28-29 0.18304639857337435 1103 30-31 0.10174834486230822 1103 32-33 0.18645699837677654 1103 34-35 0.4835904874300141 1103 36-37 0.45955988040740436 1103 38-39 0.45747029344284584 1103 40-41 0.27711687817543407 1103 42-43 0.3428389097127251 1103 44-45 0.5102387312810279 1103 46-47 0.7660047727573698 1103 48-49 0.6895146271092187 1103 50-51 0.8098090015519048 1103 52-53 0.8911144224378589 1103 54-55 0.9682783320556823 1103 56-57 0.7554595798329089 1103 58-59 0.8566573485545987 1103 60-61 0.979281804794752 1103 62-63 0.9303922986815891 1103 64-65 0.9172671671000217 1103 66-67 1.006216251885526 1103 68-69 0.9658486033930025 1103 70-71 0.7488139980859607 1103 72-73 0.5730616405245534 1103 74-75 0.6090733776381008 1103 76-77 0.7757859775354277 1103 78-79 0.8948994858183994 1103 80-81 0.7668999960745708 1103 82-83 0.6411395358117424 1103 84-85 0.8311536933757218 1103 86-87 0.6349770432011788 1103 88-89 0.6527038938036789 1103 90-91 0.6255887900378028 1103 92-93 0.5778847889700742 1103 94-95 0.8510735310217079 1103 96-97 0.727152223909485 1103 98-99 0.6265449044987008 1103 100-101 0.51819770531662 1104 1 0.21229521000094564 1104 2 0.3524610265549306 1104 3 0.19466844694628804 1104 4 0.2394990086267086 1104 5 0.2722395787959897 1104 6 0.3195034056733803 1104 7 0.2141835452576757 1104 8 0.3285045558705022 1104 9 0.4051407684546504 1104 10-11 0.4070905692879947 1104 12-13 0.3243158120514025 1104 14-15 0.04702523960281724 1104 16-17 0.19973110088777446 1104 18-19 0.164359073313193 1104 20-21 0.10356024316089929 1104 22-23 0.152489466708829 1104 24-25 0.2506835099052722 1104 26-27 0.11449125015584372 1104 28-29 -0.07762239707347618 1104 30-31 0.33025262372654396 1104 32-33 0.44046581659981143 1104 34-35 0.41942874925597806 1104 36-37 0.1909980997824121 1104 38-39 -0.022244320301616938 1104 40-41 0.11482452610257354 1104 42-43 0.21722650631278384 1104 44-45 0.11568441816657327 1104 46-47 0.055639983902679546 1104 48-49 0.19715850211042607 1104 50-51 0.08213386720424865 1104 52-53 0.2737687654609431 1104 54-55 0.22365715867243807 1104 56-57 0.04624965016643756 1104 58-59 0.17504575558216118 1104 60-61 0.12956227059761005 1104 62-63 0.205608075003596 1104 64-65 0.2789044236099727 1104 66-67 0.35181225377652225 1104 68-69 0.34657663743096023 1104 70-71 0.46838505038748224 1104 72-73 0.2314797632271386 1104 74-75 0.30830848844476577 1104 76-77 0.24650909112069996 1104 78-79 0.4117999865468178 1104 80-81 0.42680660395308223 1104 82-83 0.22742150669424177 1104 84-85 0.12048981818155369 1104 86-87 0.14700726056940994 1104 88-89 0.2131330344610909 1104 90-91 0.16957242712064868 1104 92-93 0.2741317378070143 1104 94-95 0.18974886797623824 1104 96-97 0.22080522586801976 1104 98-99 0.26084952875942946 1104 100-101 -0.16780852725677775 1105 1 0.3833172660382118 1105 2 0.20930157444517405 1105 3 0.32216911564878004 1105 4 0.33546199594364623 1105 5 0.20629204593669215 1105 6 0.5089514660646444 1105 7 0.5842588300134821 1105 8 0.5130385816824159 1105 9 0.48713808992965824 1105 10-11 0.5890229244581846 1105 12-13 0.45700147915643896 1105 14-15 0.42014451383771245 1105 16-17 0.4842164135804836 1105 18-19 0.3447469114723276 1105 20-21 0.3660582463409483 1105 22-23 0.14439793433992065 1105 24-25 0.2917618112523357 1105 26-27 0.18878061444267047 1105 28-29 0.07543617383499424 1105 30-31 0.12615904995751492 1105 32-33 -0.012249254839900914 1105 34-35 0.05075681098465168 1105 36-37 0.17556755753899012 1105 38-39 0.05281962437439347 1105 40-41 -0.16660031173707068 1105 42-43 -0.17475737609959197 1105 44-45 -0.14747764374041594 1105 46-47 0.30087246018231895 1105 48-49 0.21306154767877672 1105 50-51 0.1283316898346314 1105 52-53 0.2927339112009406 1105 54-55 0.1213691280755782 1105 56-57 -0.10918165365482224 1105 58-59 -0.02477021191889861 1105 60-61 -0.10419499225448448 1105 62-63 0.0910409948558879 1105 64-65 0.061403597019392464 1105 66-67 0.2996411532408203 1105 68-69 0.3766060231905719 1105 70-71 0.17429456936748622 1105 72-73 0.0011923233174861991 1105 74-75 0.10549450476110778 1105 76-77 0.228133936018871 1105 78-79 0.20366192362934754 1105 80-81 0.21664223316498266 1105 82-83 0.01572608582032231 1105 84-85 0.03998453914686095 1105 86-87 -0.3016370844364964 1105 88-89 -0.1804137078432575 1105 90-91 -0.23703816141184575 1105 92-93 -0.32088322441129336 1105 94-95 -0.23962105638608122 1105 96-97 -0.21773497801193997 1105 98-99 -0.13570591039236035 1105 100-101 -0.1482540874437177 1106 1 0.2883862709493137 1106 2 0.1980004609120698 1106 3 0.21532804361054403 1106 4 0.42439855869806564 1106 5 0.3116963363657348 1106 6 0.3706275872576654 1106 7 0.5720976172078522 1106 8 0.5590177817244424 1106 9 0.603474176456082 1106 10-11 0.5958749939489465 1106 12-13 0.3858465934424018 1106 14-15 0.4247732923654297 1106 16-17 0.3511196075470906 1106 18-19 0.379438402836314 1106 20-21 0.23404914854788217 1106 22-23 0.5047650303996534 1106 24-25 0.22378059336927691 1106 26-27 0.08891635911793827 1106 28-29 0.2657697960566381 1106 30-31 0.3031097025824607 1106 32-33 0.35027848500304515 1106 34-35 0.31490194234128666 1106 36-37 0.276401339524611 1106 38-39 0.221417958014122 1106 40-41 0.005280022848111798 1106 42-43 0.19824694190515402 1106 44-45 0.043071824004762505 1106 46-47 0.13569592097667282 1106 48-49 0.33188108878408684 1106 50-51 0.035102564203228326 1106 52-53 0.01832066085734141 1106 54-55 0.0951330910103394 1106 56-57 -0.006372160172318786 1106 58-59 0.19737258425727333 1106 60-61 0.07565083549805252 1106 62-63 0.1013788738029362 1106 64-65 0.033133304804493946 1106 66-67 0.2937055381275897 1106 68-69 0.4644028996951981 1106 70-71 0.28050944317581994 1106 72-73 -0.0829838744747029 1106 74-75 0.05178836325836045 1106 76-77 0.15092005581122336 1106 78-79 0.06337409676973493 1106 80-81 0.3730615720484707 1106 82-83 0.23890652720663397 1106 84-85 0.24812746882444614 1106 86-87 0.1221907840930605 1106 88-89 0.2197507615214498 1106 90-91 0.03247219479618835 1106 92-93 -0.1001602485991242 1106 94-95 0.08754850572928419 1106 96-97 0.2936066236548598 1106 98-99 0.12221006688175606 1106 100-101 -0.13663672875824417 1107 1 0.23157637159709665 1107 2 0.3923022732272976 1107 3 0.2582323340395867 1107 4 0.21027838737572324 1107 5 0.2563850980458682 1107 6 0.29373726795278543 1107 7 0.4651931302331036 1107 8 0.443809238537618 1107 9 0.5810761550162198 1107 10-11 0.5407971536587226 1107 12-13 0.2806864496364554 1107 14-15 0.13835705768985207 1107 16-17 0.13907954498004926 1107 18-19 0.1945666898585685 1107 20-21 0.49041026853084446 1107 22-23 0.44285722671605754 1107 24-25 0.5224686507606222 1107 26-27 0.530947659022118 1107 28-29 0.6478956016893065 1107 30-31 0.6118625381637841 1107 32-33 0.2881905144193908 1107 34-35 0.42901720002090116 1107 36-37 0.5500871448297104 1107 38-39 0.630597461572421 1107 40-41 0.7515477017251797 1107 42-43 0.5220273348327851 1107 44-45 0.5077509221875331 1107 46-47 0.5876200215753187 1107 48-49 0.6233215231501035 1107 50-51 0.36857102271161324 1107 52-53 0.5269400091445249 1107 54-55 0.6308552736504325 1107 56-57 0.4526275859561437 1107 58-59 0.44768582898124265 1107 60-61 0.6346432111624196 1107 62-63 0.9064546667420359 1107 64-65 0.723021028068132 1107 66-67 0.7623605922186059 1107 68-69 0.8290852736397873 1107 70-71 0.7173650394054256 1107 72-73 0.5092042525265796 1107 74-75 0.6608917053543593 1107 76-77 0.41541734921302265 1107 78-79 0.6748933132075088 1107 80-81 0.8798096413963492 1107 82-83 0.809724403038885 1107 84-85 0.4652326781046803 1107 86-87 0.4738069630806869 1107 88-89 0.5160884598074098 1107 90-91 0.5788487927537531 1107 92-93 0.6075762818777442 1107 94-95 0.6605734260873248 1107 96-97 0.5552089625487966 1107 98-99 0.8574916595571551 1107 100-101 0.5921465714079979 1108 1 0.06499336501162745 1108 2 0.21155545704585776 1108 3 0.032405362029408025 1108 4 0.1811615221347509 1108 5 -0.14183136373252125 1108 6 0.0597652767165755 1108 7 0.035282125617591475 1108 8 0.21180799468584155 1108 9 0.1490831193233575 1108 10-11 0.2557526559664751 1108 12-13 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50-51 -1.2542137651383811 2316 52-53 -1.1519488210826694 2316 54-55 -1.2657182969107765 2316 56-57 -1.1474815247912673 2316 58-59 -1.2066882800943048 2316 60-61 -1.2258270209618942 2316 62-63 -1.256604305857092 2316 64-65 -1.2101134421287938 2316 66-67 -1.4013407259096198 2316 68-69 -1.2249069478198287 2316 70-71 -1.226742100309135 2316 72-73 -0.8766430276240627 2316 74-75 -0.9866893797696221 2316 76-77 -0.728177673929018 2316 78-79 -1.5201685455468699 2316 80-81 -1.613731706297365 2316 82-83 -1.8694372040568332 2316 84-85 -1.609701767454336 2316 86-87 -1.6522335486839133 2316 88-89 -1.7856291545388956 2316 90-91 -2.2773186042509828 2316 92-93 -2.7860671371603196 2316 94-95 -2.360588779357652 2316 96-97 -2.779435459604205 2316 98-99 -3.8113836198191535 2316 100-101 -4.926050285498537 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2128.0 3 887.0 4 817.0 5 919.0 6 1027.0 7 1247.0 8 1616.0 9 2110.0 10 2561.0 11 3381.0 12 4618.0 13 5582.0 14 6548.0 15 7885.0 16 9904.0 17 12789.0 18 9664.0 19 6978.0 20 7002.0 21 7245.0 22 7850.0 23 9036.0 24 9935.0 25 11562.0 26 13150.0 27 15827.0 28 18581.0 29 22282.0 30 26725.0 31 33165.0 32 41274.0 33 52614.0 34 69061.0 35 94868.0 36 131198.0 37 173013.0 38 138556.0 39 21693.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.33136923521978 18.737394499510245 14.744032846622716 22.187203418647258 2 15.204308562415184 20.155074954247915 41.046852368196944 23.593764115139955 3 19.026114354216087 25.140610678189585 30.045464373045384 25.787810594548944 4 12.396637167782265 16.686258148736908 38.04787630364564 32.869228379835185 5 13.451694190794697 37.40743222526128 34.24342659657202 14.897446987371998 6 30.87334395476853 36.46523510841276 17.872240178472605 14.789180758346102 7 27.694623389620286 33.519242343813595 20.9856594627348 17.800474803831314 8 26.15848019175265 34.156904587627245 20.143266272021148 19.541348948598962 9 26.58344417256399 16.688123787960322 20.73438182168929 35.994050217786395 10-11 24.695022736537926 26.7034634573042 27.377055921678885 21.224457884478994 12-13 25.91933423048828 24.29755153860719 28.040017645288085 21.74309658561645 14-15 22.84767085766265 25.405161478301963 26.98839654683183 24.75877111720356 16-17 21.575849375341363 28.609629468700064 27.48157901683015 22.332942139128424 18-19 21.377954934098494 26.8329562867963 30.637564798793182 21.151523980312028 20-21 22.53035348809343 25.894839564305016 30.584963110731778 20.98984383686977 22-23 21.95833709740122 25.916749306988578 30.556906108752298 21.568007486857905 24-25 21.673412675052827 26.100962777866865 30.71059421034149 21.515030336738818 26-27 21.553120973598222 26.282980655002795 30.57982215451538 21.5840762168836 28-29 21.336620298668638 26.49725060032112 30.70365957377708 21.462469527233164 30-31 21.455568693322235 26.249359322429548 30.54811551987496 21.746956464373252 32-33 21.25928470769251 26.42426413759611 30.4777935824953 21.838657572216086 34-35 21.839560502016635 26.445743758791686 30.156519903461465 21.55817583573022 36-37 21.81473294482301 26.302646295247552 30.22875219983315 21.653868560096296 38-39 21.519406154027276 26.624216233332586 30.22441759209829 21.63196002054184 40-41 21.841992820438914 26.546411894078638 30.025768879840292 21.585826405642155 42-43 21.63632174715285 26.706387910884487 29.683400606729233 21.97388973523343 44-45 21.75012470878026 26.844332487055915 29.544131684352564 21.861411119811265 46-47 21.925628667034065 27.020325809728824 29.05361722685195 22.00042829638516 48-49 22.001250388209 27.21537936900052 28.712445219396443 22.070925023394032 50-51 21.84536031203598 27.531308645032688 28.564729906797215 22.058601136134122 52-53 22.204731442096215 27.55051604567631 28.152780780305314 22.091971731922165 54-55 21.844297568976437 27.435032640997743 28.284043667484042 22.436626122541774 56-57 21.9238872954688 27.646751960312677 28.189834182819073 22.23952656139945 58-59 22.071394062911057 27.30222559638838 28.31946564575586 22.306914694944705 60-61 21.99819240210071 27.307211970540518 28.694677837033023 21.99991779032575 62-63 21.928860390012403 27.395813669311543 28.555965582126092 22.119360358549965 64-65 21.79230782112444 27.404408507260992 28.489256312420743 22.314027359193826 66-67 21.854866951221368 27.599894433927314 28.1502083407348 22.395030274116518 68-69 21.885694182364794 27.367105945159487 28.36618149567905 22.381018376796675 70-71 21.88247300199534 27.278519895003527 28.280403535761252 22.55860356723988 72-73 21.80810866090819 27.366667123446888 28.33529495059922 22.489929265045706 74-75 21.74238797893817 27.661751799103456 28.101140654687363 22.494719567271012 76-77 21.761753764204435 27.53225645167103 28.169390849365328 22.5365989347592 78-79 21.852228904804097 27.57291954921768 28.104282127379772 22.47056941859845 80-81 21.777642571029673 27.785105238646402 27.864028509889028 22.5732236804349 82-83 21.820054802087498 27.716330910377717 27.98516271813074 22.478451569404044 84-85 21.85391641708323 27.526401029699795 27.899088608083204 22.72059394513377 86-87 21.801291603561758 27.5191547934693 27.901692362038794 22.77786124093015 88-89 21.767163385184325 27.50217364050397 27.792093887057995 22.93856908725371 90-91 21.74884853333631 27.39411913117506 28.126905256617285 22.73012707887134 92-93 21.799474457059933 27.45284249497374 27.980415734209473 22.76726731375685 94-95 21.659005587335038 27.50121932713443 27.908659836404613 22.931115249125916 96-97 21.66281178482298 27.451834279162473 27.967155087048255 22.918198848966288 98-99 21.741143689237575 27.40434900048539 27.648871116129726 23.20563619414731 100-101 21.776075031003916 27.352514288209978 27.67951547555083 23.19189520523527 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 298.0 1 249.0 2 293.0 3 384.5 4 339.0 5 333.5 6 510.5 7 980.5 8 1571.0 9 1839.0 10 1619.5 11 1376.0 12 1422.5 13 1534.5 14 1604.5 15 1639.0 16 1686.0 17 1790.0 18 1909.0 19 2032.0 20 2194.0 21 2537.0 22 2999.5 23 3614.0 24 4372.0 25 5376.0 26 6793.0 27 8363.0 28 9834.5 29 11226.0 30 12937.0 31 14900.5 32 16758.5 33 18351.5 34 20086.0 35 21775.5 36 23876.5 37 25807.0 38 26582.5 39 27416.0 40 28604.5 41 30224.0 42 31652.5 43 32720.5 44 33837.0 45 34819.0 46 35226.5 47 35854.0 48 38217.0 49 38254.5 50 35523.5 51 33700.5 52 32488.5 53 31911.5 54 30965.5 55 28459.5 56 25572.0 57 22976.0 58 21127.5 59 19060.5 60 16142.0 61 13167.0 62 10609.0 63 8313.5 64 5998.0 65 4487.0 66 3581.0 67 2863.5 68 2192.0 69 1654.0 70 1282.5 71 1008.5 72 842.0 73 761.5 74 605.5 75 451.0 76 340.0 77 226.0 78 156.5 79 111.0 80 78.5 81 55.5 82 33.5 83 20.5 84 17.0 85 12.5 86 10.5 87 7.5 88 5.0 89 3.0 90 2.0 91 1.0 92 2.5 93 4.0 94 2.5 95 1.5 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012280448916377246 2 0.010656587902641412 3 0.014107292556830062 4 0.018674401657962095 5 0.03572494230218835 6 0.05125311324603728 7 0.00578500486143391 8 0.029128006933886525 9 0.0401905600899619 10-11 0.04440244959433922 12-13 0.03455779219856573 14-15 0.03019366572415067 16-17 0.02922949824724502 18-19 0.02410418692264129 20-21 0.05424710699011272 22-23 0.01146851840950933 24-25 0.003247722027471669 26-27 0.0012686414169811207 28-29 0.0 30-31 0.0021313175805282823 32-33 0.001674606670415079 34-35 0.0 36-37 4.059652534339586E-4 38-39 0.0 40-41 9.134218202264068E-4 42-43 0.002334300207245262 44-45 0.0010656587902641412 46-47 7.104391935094274E-4 48-49 0.0012178957603018758 50-51 0.0035521959675471376 52-53 0.0025372828339622414 54-55 0.004262635161056565 56-57 4.059652534339586E-4 58-59 0.004262635161056565 60-61 0.002080571923849038 62-63 4.059652534339586E-4 64-65 6.596935368301828E-4 66-67 0.014310275183547041 68-69 0.040139814433282656 70-71 0.05186206112618821 72-73 0.015172951347094203 74-75 0.03059963097758463 76-77 0.02745340026347145 78-79 0.058205268211093814 80-81 0.08175125291026342 82-83 0.04967999788898068 84-85 0.016695321047471547 86-87 0.0176594885243772 88-89 0.021059447521886602 90-91 0.03719656634588645 92-93 0.02329225641577337 94-95 0.013346107706641387 96-97 0.01943558650815077 98-99 0.05358741345328253 100-101 0.0764229589589427 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 985306.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.01966243645472 #Duplication Level Percentage of deduplicated Percentage of total 1 80.27303331426047 51.390524955292385 2 11.886739788567482 15.219701374681305 3 3.5882988159887783 6.891650367621952 4 1.4894481129643846 3.8141586163437746 5 0.790849064522121 2.5314945074446094 6 0.43954457932667246 1.6883697356560237 7 0.29475888191800353 1.3209254884376183 8 0.2064013641489098 1.0570996527389576 9 0.14980453094190785 0.8631391952117138 >10 0.8158236647125027 9.35794224853088 >50 0.04147279209557358 1.7992237237337456 >100 0.022395585657038922 2.3379401076712703 >500 6.353355093968E-4 0.2821756311154907 >1k 6.353355093968E-4 0.8953356511610435 >5k 1.588338773492E-4 0.5503187443592922 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5412 0.5492709878961459 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4124 0.41855017629041125 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2549 0.2587013577507901 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1114 0.11306132308135745 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1018 0.10331815699894245 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.0149131335848965E-4 0.0 0.0 0.0 0.0 3 1.0149131335848965E-4 0.0 0.0 0.0 0.0 4 1.0149131335848965E-4 0.0 0.0 0.0 0.0 5 2.029826267169793E-4 0.0 0.0 0.0 0.0 6 5.074565667924482E-4 0.0 0.0 0.0 0.0 7 5.074565667924482E-4 0.0 0.0 0.0 0.0 8 5.074565667924482E-4 0.0 0.0 0.0 0.0 9 5.074565667924482E-4 0.0 0.0 0.0 0.0 10-11 5.58202223471693E-4 0.0 0.0 0.0 0.0 12-13 7.611848501886723E-4 0.0 0.0 0.0 0.0 14-15 0.0010149131335848965 0.0 0.0 0.0 0.0 16-17 0.0010656587902641412 0.0 0.0 2.029826267169793E-4 0.0 18-19 0.0012686414169811207 0.0 0.0 2.029826267169793E-4 0.0 20-21 0.001420878387018855 0.0 0.0 2.029826267169793E-4 0.0 22-23 0.0015223697003773446 0.0 0.0 2.029826267169793E-4 0.0 24-25 0.0018268436404528137 0.0 0.0 3.552195967547138E-4 0.0 26-27 0.0018268436404528137 0.0 0.0 5.074565667924482E-4 0.0 28-29 0.002080571923849038 0.0 0.0 8.119305068679172E-4 0.0 30-31 0.002232808893886772 0.0 0.0 0.002029826267169793 0.0 32-33 0.002232808893886772 0.0 0.0 0.0041103981910188304 0.0 34-35 0.002283554550566017 0.0 0.0 0.006444698398264092 0.0 36-37 0.0024357915206037516 0.0 0.0 0.008931235575547088 0.0 38-39 0.0026387741473207307 0.0 0.0 0.011417772752830086 0.0 40-41 0.0027402654606792204 0.0 0.0 0.01441176649690553 0.0 42-43 0.003247722027471669 0.0 0.0 0.021414667118641317 0.0 44-45 0.0035521959675471376 0.0 0.0 0.03029515703750916 0.0 46-47 0.004059652534339586 0.0 0.0 0.04150994716362227 0.0 48-49 0.004364126474415054 0.0 0.0 0.06739023207003712 0.0 50-51 0.004364126474415054 0.0 0.0 0.0925093321262633 0.0 52-53 0.004364126474415054 0.0 0.0 0.11955676713630081 0.0 54-55 0.004364126474415054 0.0 0.0 0.17065764341230033 0.0 56-57 0.004668600414490524 0.0 0.0 0.220540623927998 0.0 58-59 0.004668600414490524 0.0 0.0 0.28524133619403513 0.0 60-61 0.004719346071169769 0.0 0.0 0.36272995394324203 0.0 62-63 0.004820837384528258 0.0 0.0 0.4551885404128261 0.0 64-65 0.004973074354565993 0.0 0.0 0.534554747459165 0.0 66-67 0.004973074354565993 0.0 0.0 0.6455354986166735 0.0 68-69 0.004973074354565993 0.0 0.0 0.791378515912823 0.0 70-71 0.005125311324603727 0.0 0.0 0.8906370203774259 0.0 72-73 0.005226802637962217 0.0 0.0 1.0280055130081416 0.0 74-75 0.005277548294641461 0.0 0.0 1.19074683397848 0.0 76-77 0.005277548294641461 0.0 0.0 1.3289272571160633 0.0 78-79 0.005277548294641461 0.0 0.0 1.4592421034683642 0.0 80-81 0.00568351354807542 0.0 0.0 1.645377172167834 0.0 82-83 0.0058864961747924 0.0 0.0 1.901541247084662 0.0 84-85 0.00598798748815089 0.0 0.0 2.1852094679216405 0.0 86-87 0.006140224458188624 0.0 0.0 2.5267277373729584 0.0 88-89 0.006190970114867868 0.0 0.0 2.8985411638617853 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4175 0.0 37.206158 1 GTATCAA 6520 0.0 34.753162 1 ATCAACG 7890 0.0 28.536642 3 TATCAAC 7965 0.0 28.209728 2 TCAACGC 7975 0.0 28.172924 4 CAACGCA 8105 0.0 27.779652 5 AACGCAG 8210 0.0 27.483624 6 GTACATG 5185 0.0 24.919752 1 GAGTACT 4955 0.0 24.733042 12-13 ACGCAGA 9050 0.0 24.7227 7 CGCAGAG 9090 0.0 24.56165 8 TACATGG 5195 0.0 24.048819 2 ACATGGG 5205 0.0 23.271315 3 GCAGAGT 9465 0.0 22.934912 9 GTACTTT 5365 0.0 22.711834 14-15 AGTACTT 5165 0.0 22.531872 12-13 TAGCCCT 610 0.0 21.803675 4 AGAGTAC 7805 0.0 21.483414 10-11 GTCCTAC 155 3.9036167E-5 21.453093 1 CATGGGG 2255 0.0 20.64339 4 >>END_MODULE