FastQCFastQC Report
Thu 26 May 2016
SRR936751_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936751_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences985306
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60760.6166612199661831No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51730.525014564003467No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33130.3362407211566762No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13260.13457748151335727No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13110.13305511181297994No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12380.12564624593781018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43250.037.6776471
GTATCAA66350.033.4388771
GTCTACG856.399443E-427.9464631
TATCAAC84400.026.2848852
ATCAACG84600.026.0516473
TCAACGC85950.025.6411574
CAACGCA87450.025.2556575
AACGCAG89100.024.8400066
AGTACTT61300.022.23924412-13
GAGTACT59450.022.21219812-13
ACGCAGA99900.022.0595197
GTACATG48650.021.8746411
CGCAGAG101300.021.7546488
TCGCAAG1100.002878157221.5895145
GTAGCAC3101.0913936E-1121.4513153
TACATGG49850.021.1553132
AGAGTAC88350.021.02121210-11
ACATGGG49950.020.8255393
GCAGAGT105350.020.6929139
GTACTTT64200.020.60628914-15
Produced by FastQC (version 0.11.5)