##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936751_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 985306 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.866928649576884 31.0 31.0 34.0 30.0 34.0 2 32.00418448684977 31.0 31.0 34.0 30.0 34.0 3 32.0968288024228 33.0 31.0 34.0 30.0 34.0 4 35.842751388908624 37.0 35.0 37.0 35.0 37.0 5 35.69995818557889 37.0 35.0 37.0 33.0 37.0 6 35.74962803433654 37.0 35.0 37.0 33.0 37.0 7 35.66723028176018 37.0 35.0 37.0 33.0 37.0 8 35.64145859255906 37.0 35.0 37.0 33.0 37.0 9 37.24481125660455 39.0 37.0 39.0 33.0 39.0 10-11 37.141577844852264 39.0 37.0 39.0 33.0 39.0 12-13 37.017335731234766 39.0 37.0 39.0 33.0 39.0 14-15 38.087529660836324 40.0 37.0 41.0 33.0 41.0 16-17 38.059890531469414 40.0 37.0 41.0 33.0 41.0 18-19 38.21998800372676 40.0 37.5 41.0 33.5 41.0 20-21 38.238672047059495 40.0 38.0 41.0 33.0 41.0 22-23 38.12879196919536 40.0 37.0 41.0 33.0 41.0 24-25 38.00980406087043 40.0 37.0 41.0 33.0 41.0 26-27 37.79468713272831 40.0 37.0 41.0 33.0 41.0 28-29 37.78585485118329 40.0 37.0 41.0 33.0 41.0 30-31 37.621809874292865 39.5 37.0 41.0 32.5 41.0 32-33 37.38050615747798 39.0 36.0 41.0 32.0 41.0 34-35 37.15770938165403 39.0 36.0 41.0 31.5 41.0 36-37 36.954397415625195 39.0 35.0 41.0 31.0 41.0 38-39 36.73002803190075 39.0 35.0 41.0 31.0 41.0 40-41 36.46580097959416 39.0 35.0 40.0 30.0 41.0 42-43 36.15529693313549 39.0 35.0 40.0 30.0 41.0 44-45 35.78939892784577 38.5 35.0 40.0 29.0 41.0 46-47 35.32121645458365 38.0 35.0 40.0 26.5 41.0 48-49 34.8524920177082 38.0 34.0 40.0 25.5 41.0 50-51 34.51981414910698 38.0 34.0 40.0 24.0 41.0 52-53 34.26244587975715 38.0 33.5 40.0 23.5 41.0 54-55 33.92857548822396 38.0 33.0 40.0 23.0 41.0 56-57 33.5875727946445 37.5 33.0 40.0 22.0 41.0 58-59 33.211701237991036 37.0 32.5 40.0 20.5 41.0 60-61 32.83867752759041 36.5 32.0 39.0 19.5 40.0 62-63 32.511774007262716 36.0 31.0 39.0 18.0 40.0 64-65 32.19629739390605 35.5 31.0 39.0 17.0 40.0 66-67 31.752151108386634 35.0 31.0 38.5 13.5 40.0 68-69 31.286214130432576 35.0 30.5 38.0 9.5 40.0 70-71 30.78351141675784 34.5 29.5 37.5 7.5 39.5 72-73 30.24225621279075 34.0 29.0 37.0 7.0 39.0 74-75 29.716220646174893 34.0 29.0 36.0 4.0 39.0 76-77 28.342837656524978 32.0 27.0 34.5 2.0 37.0 78-79 29.12463184026079 34.0 29.0 35.0 2.0 37.0 80-81 28.961595687025145 34.0 29.0 35.0 2.0 37.0 82-83 28.543485475578144 33.5 28.0 35.0 2.0 36.5 84-85 28.08878866057854 33.0 27.0 35.0 2.0 36.0 86-87 27.650984059774323 33.0 26.0 35.0 2.0 36.0 88-89 27.138823878064276 33.0 26.0 35.0 2.0 35.0 90-91 26.550531510008057 32.0 25.0 34.0 2.0 35.0 92-93 25.93510188712948 32.0 24.0 34.0 2.0 35.0 94-95 25.16844767006392 31.5 20.5 34.0 2.0 35.0 96-97 24.336324451490196 31.0 14.0 34.0 2.0 35.0 98-99 23.14986917769708 31.0 2.0 34.0 2.0 35.0 100-101 19.535561033831115 26.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2290656979182053 1101 2 0.2847556545109633 1101 3 0.18513300556923795 1101 4 0.15884104687044243 1101 5 0.11265770764546801 1101 6 0.09891996568408246 1101 7 0.08482556453778045 1101 8 -0.024517766205072178 1101 9 0.08513589094515339 1101 10-11 0.1119030831790937 1101 12-13 0.2568245702646266 1101 14-15 0.17121365591892612 1101 16-17 0.19992736536944733 1101 18-19 0.15444357876368997 1101 20-21 0.033953107282947315 1101 22-23 0.13733909262791855 1101 24-25 0.2324697737527046 1101 26-27 0.00443983221602906 1101 28-29 0.269059410842452 1101 30-31 0.4405690015108945 1101 32-33 0.29540715309587284 1101 34-35 0.2535768170305417 1101 36-37 0.2827522938973246 1101 38-39 0.39495136950306176 1101 40-41 0.4176733489523201 1101 42-43 0.6663037430801211 1101 44-45 0.6310737506643989 1101 46-47 0.6639792549493038 1101 48-49 0.7650417009943311 1101 50-51 0.7089223298978737 1101 52-53 0.8344264796245113 1101 54-55 0.8140578687791233 1101 56-57 0.7324751407646133 1101 58-59 0.672109453005433 1101 60-61 0.6897747891530983 1101 62-63 0.7038092309979049 1101 64-65 0.7058814980096351 1101 66-67 0.3477779638649423 1101 68-69 0.5182166885123607 1101 70-71 0.5000344459658557 1101 72-73 0.6829549018548597 1101 74-75 0.7474403641159881 1101 76-77 0.6624356429831266 1101 78-79 0.76022247461567 1101 80-81 0.7839006348848336 1101 82-83 0.8408307164046036 1101 84-85 0.780689610413603 1101 86-87 0.911887095701676 1101 88-89 0.6717257661098017 1101 90-91 0.8355603062502972 1101 92-93 0.7233226215836659 1101 94-95 0.8399575319951857 1101 96-97 0.6740183611528678 1101 98-99 0.30643755089534963 1101 100-101 0.4880372582426524 1102 1 0.17725133580972496 1102 2 0.2882443227055127 1102 3 0.20613504274774996 1102 4 0.10531418157886918 1102 5 0.11162001984307324 1102 6 0.1790956723303765 1102 7 0.2710809757931898 1102 8 0.17757670794822644 1102 9 0.16035870734444302 1102 10-11 0.2976110027105392 1102 12-13 0.30176982109222905 1102 14-15 0.3618076207775971 1102 16-17 0.3468143019622687 1102 18-19 0.23440920137637278 1102 20-21 0.17824582436977465 1102 22-23 0.13192147103382723 1102 24-25 0.15237046130044973 1102 26-27 0.3743787168607966 1102 28-29 0.31753789314446834 1102 30-31 0.4159509724810988 1102 32-33 0.5018624624923618 1102 34-35 0.6439193176671623 1102 36-37 0.6470348250916231 1102 38-39 0.6436749498442893 1102 40-41 0.7625040084888681 1102 42-43 0.7663132923543827 1102 44-45 0.950013144603794 1102 46-47 0.8423501487613763 1102 48-49 0.9174258364667054 1102 50-51 0.929478734277815 1102 52-53 1.0452330749899303 1102 54-55 1.0469583016795596 1102 56-57 1.0681575955647205 1102 58-59 0.8980739297051983 1102 60-61 0.7781119421961264 1102 62-63 0.6788697097348546 1102 64-65 0.7076576630210916 1102 66-67 0.8172839480768133 1102 68-69 0.6582090490929566 1102 70-71 0.5012885839846994 1102 72-73 0.5270884643707738 1102 74-75 0.5417458135684967 1102 76-77 0.5790354647300084 1102 78-79 0.5865466406457145 1102 80-81 0.8648020863745209 1102 82-83 0.6974770112861925 1102 84-85 0.4989923860693182 1102 86-87 0.8387428379985984 1102 88-89 0.518838702193321 1102 90-91 0.18910945318179628 1102 92-93 0.3710562604937735 1102 94-95 0.3950504782646007 1102 96-97 0.16880127431813463 1102 98-99 0.05636115670131048 1102 100-101 -0.00289093121494588 1103 1 0.16184433718938251 1103 2 0.2418209772479294 1103 3 0.24217668328455844 1103 4 0.10287929717734556 1103 5 0.14577362158227913 1103 6 0.12555497246834335 1103 7 0.12862184288629663 1103 8 0.1810902327611359 1103 9 0.06301015598359783 1103 10-11 0.08589061306214063 1103 12-13 0.16075556464338092 1103 14-15 0.29564264732552914 1103 16-17 0.2523881135764654 1103 18-19 0.13561660971284084 1103 20-21 0.1535817497847205 1103 22-23 0.13188551635344936 1103 24-25 0.15181525414295294 1103 26-27 0.0733285057315598 1103 28-29 0.017260289565719233 1103 30-31 0.22470384676513788 1103 32-33 0.3117107002949453 1103 34-35 0.1805345607793285 1103 36-37 0.2550836565645085 1103 38-39 0.1631966689797011 1103 40-41 0.2637281399324891 1103 42-43 0.3045264283550466 1103 44-45 0.5452796691240565 1103 46-47 0.5873511939555769 1103 48-49 0.7111964790520915 1103 50-51 0.8443255088085166 1103 52-53 0.7416534939037689 1103 54-55 0.7641438027456573 1103 56-57 0.6839835080192529 1103 58-59 0.6153547524820766 1103 60-61 0.39630835508964424 1103 62-63 0.6064820681110348 1103 64-65 0.6126428212634956 1103 66-67 0.5391193090868853 1103 68-69 0.5717664720621443 1103 70-71 0.5442351954651095 1103 72-73 0.755484300317093 1103 74-75 0.6264784528928828 1103 76-77 0.4562059149996678 1103 78-79 0.4019143399642502 1103 80-81 0.5917497012413655 1103 82-83 0.4636988433025806 1103 84-85 0.4419356529283043 1103 86-87 0.6704816230648625 1103 88-89 0.5865915346695978 1103 90-91 0.5205472223193048 1103 92-93 0.7116750154584466 1103 94-95 0.5860174919357419 1103 96-97 0.8810919219204649 1103 98-99 0.808836594637679 1103 100-101 0.6058569261369477 1104 1 0.19832438041218126 1104 2 0.27044643431939264 1104 3 0.20897925735667044 1104 4 0.20606832939184017 1104 5 0.16841812370000042 1104 6 0.09079573697161436 1104 7 0.1718782177669027 1104 8 0.14935003266272417 1104 9 0.16656426649117861 1104 10-11 0.23809796672691874 1104 12-13 0.23023351426180483 1104 14-15 0.18163093471855518 1104 16-17 0.2600657563901905 1104 18-19 0.1967664519689194 1104 20-21 0.2025724781082019 1104 22-23 0.22709492503081208 1104 24-25 0.18994856946679306 1104 26-27 0.1592311193602498 1104 28-29 0.29793005000720996 1104 30-31 0.45180531421779335 1104 32-33 0.42203052971924393 1104 34-35 0.38427699920131175 1104 36-37 0.20310032821594604 1104 38-39 0.4335047279093587 1104 40-41 0.4028401429426438 1104 42-43 0.6121654793830373 1104 44-45 0.671344754170704 1104 46-47 0.45607563895745784 1104 48-49 0.40238597436801626 1104 50-51 0.29006912427995957 1104 52-53 0.2211218920552156 1104 54-55 0.11412055119475184 1104 56-57 0.32568830015424766 1104 58-59 0.4848224752772765 1104 60-61 0.29207445027628154 1104 62-63 0.20203282113325827 1104 64-65 0.4113206666841016 1104 66-67 0.5889926315501981 1104 68-69 0.5818246491791434 1104 70-71 0.4356992713365635 1104 72-73 0.4546256330844116 1104 74-75 0.6597376256779555 1104 76-77 0.4809100607222483 1104 78-79 0.6140622622789849 1104 80-81 0.7094260898220526 1104 82-83 0.7597696206376234 1104 84-85 0.4972255254789282 1104 86-87 0.5430953482334608 1104 88-89 0.5460095705406616 1104 90-91 0.7072951890145909 1104 92-93 0.9133531792612821 1104 94-95 0.8277589462671848 1104 96-97 0.822410410280213 1104 98-99 0.6562103271387123 1104 100-101 0.7127953432948573 1105 1 0.11390858491626332 1105 2 0.25029155539169423 1105 3 0.19817599706050615 1105 4 0.15908362038887702 1105 5 0.06830407277459472 1105 6 0.20720105241344555 1105 7 0.20379854557972976 1105 8 0.17551208899719484 1105 9 0.09127486691171782 1105 10-11 0.1756952536522931 1105 12-13 0.0567713906855829 1105 14-15 0.13963711464410977 1105 16-17 0.2131616167502557 1105 18-19 0.2723716277262227 1105 20-21 0.2602975243601193 1105 22-23 0.11382345596572918 1105 24-25 0.23511942295407806 1105 26-27 0.22270468517915276 1105 28-29 0.07953019006323814 1105 30-31 0.11781094060627595 1105 32-33 0.23032281214945982 1105 34-35 0.015934071844689868 1105 36-37 0.2667471065559326 1105 38-39 0.2767480928946142 1105 40-41 0.18520395053464966 1105 42-43 0.3646169241519246 1105 44-45 0.19722168319164268 1105 46-47 0.26171772337742993 1105 48-49 0.16297422823720353 1105 50-51 0.19542218062186834 1105 52-53 0.1246750241834036 1105 54-55 0.1527464790194628 1105 56-57 0.42617942498613814 1105 58-59 0.3204291649027269 1105 60-61 0.1284596674931393 1105 62-63 0.21634375107552728 1105 64-65 0.22118695404784816 1105 66-67 0.35921623823425364 1105 68-69 0.1110337134414543 1105 70-71 -4.180469435333123E-4 1105 72-73 0.07460850687053266 1105 74-75 0.22612654862113857 1105 76-77 0.07304263656425647 1105 78-79 -0.0041360117255777595 1105 80-81 0.23148143052597447 1105 82-83 0.20203667998125852 1105 84-85 0.31954802211684097 1105 86-87 0.4697175554717923 1105 88-89 0.5802055765292664 1105 90-91 0.7716048651396825 1105 92-93 0.848513881473199 1105 94-95 0.8016029268302347 1105 96-97 0.646976078722659 1105 98-99 0.8207633831091137 1105 100-101 0.7921684718566269 1106 1 -0.0029658843609041696 1106 2 0.1544058063393301 1106 3 0.0911209944780218 1106 4 0.05822549080934181 1106 5 0.17616227238765703 1106 6 0.04680129227828189 1106 7 0.09633756738642063 1106 8 0.05012252749462931 1106 9 0.07576572315914376 1106 10-11 0.04170059589738884 1106 12-13 0.034319352709900386 1106 14-15 0.1506255394149676 1106 16-17 0.09583220310597085 1106 18-19 0.055925347819716364 1106 20-21 0.04685748838038961 1106 22-23 -0.0014886637740971764 1106 24-25 0.19657909677291485 1106 26-27 0.09491948457687016 1106 28-29 0.05396056579311903 1106 30-31 0.042642258287116874 1106 32-33 0.06439806317589358 1106 34-35 0.13445460261129227 1106 36-37 0.3250546697393091 1106 38-39 0.4719022651420772 1106 40-41 0.11862668604427284 1106 42-43 0.32784002033422155 1106 44-45 0.2898782300134428 1106 46-47 0.29952543319350156 1106 48-49 0.3717709865602501 1106 50-51 0.38575416143267915 1106 52-53 0.26175307217388166 1106 54-55 0.29613684590760414 1106 56-57 0.2497124414177634 1106 58-59 0.17585014300849622 1106 60-61 0.051490631066954506 1106 62-63 0.14585254247189994 1106 64-65 0.4055477558442071 1106 66-67 0.013579983627359127 1106 68-69 0.08680074472018262 1106 70-71 0.2694848800202472 1106 72-73 0.2418343474917286 1106 74-75 0.2122410337433891 1106 76-77 -0.07479020872291997 1106 78-79 -0.15818296482937555 1106 80-81 0.007451011900560189 1106 82-83 0.10050256271011193 1106 84-85 0.2610289905054586 1106 86-87 0.2824919446481111 1106 88-89 -0.011754738534072828 1106 90-91 -0.1492364563929982 1106 92-93 -0.05345258340936354 1106 94-95 0.15513163182275136 1106 96-97 0.4666755801714757 1106 98-99 0.5896650494693851 1106 100-101 0.3972340845186899 1107 1 0.08529951786170287 1107 2 0.17783569275229283 1107 3 0.02676046413218458 1107 4 0.09435714367685932 1107 5 0.03425627037306356 1107 6 0.07269618946030221 1107 7 0.05659438711305853 1107 8 0.030361494319350868 1107 9 0.09829477507177842 1107 10-11 0.21844841956313843 1107 12-13 0.1536266928732033 1107 14-15 0.26537957064935114 1107 16-17 0.3121471348472795 1107 18-19 0.2886682250851109 1107 20-21 0.10732395686347473 1107 22-23 0.04285696185546328 1107 24-25 0.1832098938476605 1107 26-27 0.16421822586539037 1107 28-29 0.28452915615091 1107 30-31 0.49132335468459587 1107 32-33 0.2746241726354697 1107 34-35 0.476849512077429 1107 36-37 0.447392089585513 1107 38-39 0.4931414858383434 1107 40-41 0.5714147688227698 1107 42-43 0.7047901222923088 1107 44-45 0.7645115494247818 1107 46-47 0.7780446250630675 1107 48-49 0.8940625457409794 1107 50-51 0.486627151957542 1107 52-53 0.5455896444167152 1107 54-55 0.42331420384191176 1107 56-57 0.6055953001750964 1107 58-59 0.6022888166525391 1107 60-61 0.6834424118529725 1107 62-63 0.4581268220090422 1107 64-65 0.6867514345247336 1107 66-67 0.6654361121682513 1107 68-69 0.6791444704078842 1107 70-71 0.5924299536839435 1107 72-73 0.5608075599494491 1107 74-75 0.3132327706342011 1107 76-77 0.2247986831687463 1107 78-79 0.3927633208661909 1107 80-81 0.5872854221405888 1107 82-83 0.8002503290500194 1107 84-85 0.8575656913219412 1107 86-87 1.153357319026739 1107 88-89 1.1056567544762714 1107 90-91 0.8980737336830806 1107 92-93 0.9116155114894546 1107 94-95 1.0837486870766604 1107 96-97 1.1681472161688227 1107 98-99 1.4377229321807299 1107 100-101 1.1518344825237499 1108 1 -0.1368756126795958 1108 2 -0.0016278093534154436 1108 3 -0.2357759201106795 1108 4 -0.11694296555479156 1108 5 0.0818383361772419 1108 6 -0.07725977061016209 1108 7 -0.039269805740794084 1108 8 -0.07401629566543022 1108 9 -0.14011277896000252 1108 10-11 -0.18405128052336295 1108 12-13 -0.15646729054631692 1108 14-15 -0.11733017938063739 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-1.0245538467874127 2316 66-67 -1.127975006568878 2316 68-69 -1.42450194037648 2316 70-71 -1.042137035035413 2316 72-73 -0.8057341573129229 2316 74-75 -1.1622789059756222 2316 76-77 -1.2924742520863397 2316 78-79 -1.3120129141090828 2316 80-81 -1.1870929105767978 2316 82-83 -1.230362275001152 2316 84-85 -1.0027813512488493 2316 86-87 -1.4138425824216192 2316 88-89 -1.4284227555812734 2316 90-91 -1.6511404929504998 2316 92-93 -1.4396155041204217 2316 94-95 -1.2862159399229682 2316 96-97 -1.0177194223402317 2316 98-99 -0.8863359876228678 2316 100-101 -0.8155642820610147 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 9.0 4 14.0 5 38.0 6 99.0 7 217.0 8 525.0 9 962.0 10 1550.0 11 2548.0 12 3524.0 13 4997.0 14 6480.0 15 8628.0 16 11102.0 17 13741.0 18 12011.0 19 7973.0 20 6917.0 21 7385.0 22 7743.0 23 8728.0 24 10181.0 25 11918.0 26 14606.0 27 17921.0 28 21398.0 29 26105.0 30 32075.0 31 38797.0 32 47942.0 33 60667.0 34 78773.0 35 105179.0 36 144908.0 37 175596.0 38 90433.0 39 3612.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.640928380966685 19.920949980663806 15.488038457205091 21.950083181164416 2 15.591701261659951 21.10900214167538 39.7119395864841 23.587357010180572 3 19.182741348598572 25.4674725575872 30.493735060822434 24.856051032991793 4 12.854077819479432 17.685267317970254 37.3106425821014 32.15001228044891 5 13.296842267656181 38.05507965377807 33.92197421198785 14.726103866577892 6 30.949167060791265 37.27278632221868 17.044248182798032 14.733798434192016 7 27.415904127051142 34.05639274782199 20.655959987983405 17.87174313714346 8 26.2844870982933 34.82801107484451 19.562649193744825 19.324852633117356 9 26.54150081294542 16.75966654014083 20.494952836986684 36.203879809927066 10-11 24.842891446921058 27.240116268448585 26.835876367341722 21.08111591728864 12-13 26.02425033441388 24.720898888264152 27.850231298703143 21.404619478618827 14-15 22.889729016543185 25.76240738861261 26.928194458540545 24.419669136303664 16-17 21.310451293876923 29.03504266911341 27.566693326404508 22.087812710605164 18-19 21.207266475318992 26.911768287979605 31.04577120285154 20.835194033849863 20-21 22.256334736118845 25.94256681837996 31.206234842837443 20.594863602663754 22-23 21.787601485227647 25.86908500781739 31.212514722621 21.130798784333965 24-25 21.64397992903713 26.15066010084197 31.241931424210197 20.963428545910702 26-27 21.444385974082124 26.288066277988175 31.16701486697767 21.10053288095203 28-29 21.19037131611905 26.58986142376074 31.24780525034862 20.971962009771584 30-31 21.47050569851533 26.303193797222267 31.01820206119859 21.208098443063818 32-33 21.261706193942974 26.372391702164588 30.959311619466355 21.40659048442608 34-35 21.775221976328954 26.392969019985358 30.71572871719325 21.11608028649244 36-37 21.811070891930388 26.225178131839638 30.774929957560953 21.188821018669017 38-39 21.640029616833488 26.590496528547813 30.694681200364784 21.07479265425392 40-41 21.678037076806596 26.590165897700818 30.461450554447044 21.270346471045542 42-43 21.719614994450843 26.720099626047094 30.062433743756245 21.497851635745818 44-45 21.85537168483355 26.900722497078196 29.74524074016748 21.49866507792078 46-47 21.98301847344886 27.26000856586685 29.182000312593242 21.57497264809105 48-49 22.271898018578522 27.363712253654427 28.77021955743433 21.594170170332724 50-51 22.070411614018994 27.695190867275894 28.50936575147266 21.725031767232455 52-53 22.399139753516444 27.742543126502717 28.113395020191838 21.744922099789 54-55 22.024996308251605 27.68750402793854 28.34141289173764 21.946086772072213 56-57 22.139469119248716 27.873878519234303 28.12915844915666 21.85749391236032 58-59 22.271469437321528 27.49643882414972 28.229480294150335 22.002611444378417 60-61 22.168722878530833 27.450288830483938 28.59378655419625 21.78720173678898 62-63 22.033261832521656 27.623823522396908 28.578250254870706 21.764664390210733 64-65 22.02965184581852 27.613922107447976 28.46962880639014 21.88679724034336 66-67 22.114848361980552 27.759478042262664 28.14733319767114 21.978340398085642 68-69 22.14097624075199 27.592018305465604 28.30496321094674 21.962042242835665 70-71 22.098767875548276 27.5649467469915 28.20706836187179 22.129217015588438 72-73 22.110729488280324 27.605387783603614 28.15934986084976 22.124532867266304 74-75 22.01793564947584 27.917181978686507 27.863286727304743 22.201595644532908 76-77 22.021244420447093 27.781301824633054 28.07649290465587 22.120960850263984 78-79 22.033393402415317 27.82935779537165 27.873508824369743 22.26373997784329 80-81 21.99320597151867 28.044651402600486 27.703884288377782 22.258258337503058 82-83 22.09123646048166 27.912569626008864 27.730841683041298 22.26535223046818 84-85 21.96346197762046 27.88871127350232 27.648727512623378 22.49909923625384 86-87 21.905526962936047 27.81821115866586 27.562501458950955 22.713760419447134 88-89 21.871181273064604 27.77115244865398 27.522947884653643 22.834718393627774 90-91 21.831256109109844 27.83196255819831 27.74522969237999 22.591551640311852 92-93 21.860544896046314 27.677272383573854 27.700616226711343 22.76156649366849 94-95 21.790216177813864 27.753932812341418 27.497665685578 22.95818532426672 96-97 21.86171888994722 27.689866432153714 27.497032643208268 22.9513820346908 98-99 21.822376219937965 27.65163971030026 27.242980789529238 23.28300328023254 100-101 21.621838325668822 27.70200664381692 27.19945761445766 23.476697416056602 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 580.0 1 417.0 2 403.5 3 509.0 4 389.5 5 357.5 6 560.5 7 1057.0 8 1624.5 9 1921.5 10 1756.5 11 1443.5 12 1476.5 13 1589.5 14 1692.0 15 1855.5 16 1984.5 17 2074.5 18 2213.5 19 2410.5 20 2593.5 21 2894.0 22 3298.0 23 3907.5 24 4738.0 25 5712.5 26 7085.0 27 8703.0 28 10064.0 29 11489.0 30 13262.5 31 15240.0 32 16981.5 33 18309.5 34 19888.5 35 21743.5 36 23906.0 37 25751.0 38 26834.5 39 27546.0 40 28519.0 41 30175.5 42 31654.0 43 32663.0 44 33738.5 45 34669.5 46 34561.5 47 35302.5 48 38312.5 49 38029.0 50 35056.5 51 33290.0 52 32012.0 53 31415.5 54 30388.5 55 27954.0 56 24847.5 57 22388.5 58 20961.0 59 19141.0 60 15943.5 61 12881.0 62 10604.5 63 8294.5 64 5995.5 65 4402.5 66 3443.0 67 2805.0 68 2201.0 69 1637.5 70 1254.5 71 983.0 72 822.5 73 741.0 74 608.0 75 473.0 76 354.5 77 237.5 78 164.0 79 117.5 80 88.0 81 72.0 82 52.0 83 31.5 84 18.5 85 11.0 86 6.0 87 2.5 88 1.5 89 1.5 90 1.0 91 0.5 92 1.0 93 1.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010859570529358393 2 0.009743166082415006 3 0.006190970114867869 4 0.0 5 0.004262635161056565 6 0.0 7 2.029826267169793E-4 8 0.0010149131335848965 9 0.0 10-11 0.0 12-13 0.0 14-15 6.089478801509379E-4 16-17 0.005074565667924483 18-19 0.0014208783870188549 20-21 4.567109101132034E-4 22-23 2.537282833962241E-4 24-25 2.029826267169793E-4 26-27 1.5223697003773447E-4 28-29 0.0 30-31 5.58202223471693E-4 32-33 0.0037551785942641167 34-35 9.641674769056517E-4 36-37 0.0010656587902641412 38-39 0.005632767891396176 40-41 0.0 42-43 0.0021820632372075276 44-45 0.00431338081773581 46-47 0.0 48-49 0.002892502430716955 50-51 0.0014208783870188549 52-53 7.104391935094274E-4 54-55 5.0745656679244825E-5 56-57 0.01146851840950933 58-59 0.0027910111173584653 60-61 0.0040089068776603416 62-63 2.537282833962241E-4 64-65 0.003907415564301851 66-67 0.0015731153570565893 68-69 0.0026895198039999756 70-71 0.005734259204754665 72-73 0.004059652534339586 74-75 0.006241715771547113 76-77 0.0012178957603018758 78-79 0.005226802637962217 80-81 0.0029432480873961995 82-83 0.004262635161056565 84-85 0.0031462307141131786 86-87 9.134218202264068E-4 88-89 0.0030447394007546893 90-91 0.009844657395773497 92-93 0.0036029416242263825 94-95 6.089478801509379E-4 96-97 1.5223697003773447E-4 98-99 0.0010149131335848965 100-101 0.0029432480873961995 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 985306.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.67965865275602 #Duplication Level Percentage of deduplicated Percentage of total 1 78.86101649501026 48.64120578421595 2 12.652508931749518 15.608048640225142 3 3.822133036434612 7.072435830381263 4 1.6567538711661036 4.087520529806295 5 0.8611304908173076 2.65571173645459 6 0.5100328265871477 1.8875190387357348 7 0.3026000413314224 1.3064987080342396 8 0.22444752071538593 1.107507717054591 9 0.16650852074582828 0.9243169850140229 >10 0.8645835993657506 9.678871754549192 >50 0.05203251960702689 2.1557930014233726 >100 0.02477546354429553 2.7979166619882574 >500 4.922276418002861E-4 0.21080260594739308 >1k 6.563035224003815E-4 0.7274359728995974 >5k 3.2815176120019077E-4 1.138415033270391 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6076 0.6166612199661831 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5173 0.525014564003467 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3313 0.3362407211566762 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1326 0.13457748151335727 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1311 0.13305511181297994 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1238 0.12564624593781018 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.0149131335848965E-4 0.0 0.0 0.0 0.0 3 1.0149131335848965E-4 0.0 0.0 0.0 0.0 4 1.0149131335848965E-4 0.0 0.0 0.0 0.0 5 1.0149131335848965E-4 0.0 0.0 0.0 0.0 6 1.0149131335848965E-4 0.0 0.0 0.0 0.0 7 1.0149131335848965E-4 0.0 0.0 0.0 0.0 8 2.029826267169793E-4 0.0 0.0 0.0 0.0 9 2.029826267169793E-4 0.0 0.0 0.0 0.0 10-11 2.029826267169793E-4 0.0 0.0 0.0 0.0 12-13 3.552195967547138E-4 0.0 0.0 0.0 0.0 14-15 4.059652534339586E-4 0.0 0.0 0.0 0.0 16-17 4.567109101132034E-4 0.0 0.0 2.029826267169793E-4 0.0 18-19 7.104391935094275E-4 0.0 0.0 2.029826267169793E-4 0.0 20-21 8.119305068679172E-4 0.0 0.0 2.029826267169793E-4 0.0 22-23 9.134218202264068E-4 0.0 0.0 2.029826267169793E-4 0.0 24-25 9.134218202264068E-4 0.0 0.0 3.552195967547138E-4 0.0 26-27 9.134218202264068E-4 0.0 0.0 4.059652534339586E-4 0.0 28-29 0.0010149131335848965 0.0 0.0 7.104391935094275E-4 0.0 30-31 0.0012178957603018758 0.0 0.0 0.0019283349538113032 0.0 32-33 0.0012178957603018758 0.0 0.0 0.004008906877660341 0.0 34-35 0.0013193870736603653 0.0 0.0 0.006393952741584848 0.0 36-37 0.001420878387018855 0.0 0.0 0.008778998605509354 0.0 38-39 0.0014716240436980998 0.0 0.0 0.011316281439471595 0.0 40-41 0.0015223697003773446 0.0 0.0 0.01441176649690553 0.0 42-43 0.0019283349538113032 0.0 0.0 0.02146541277532056 0.0 44-45 0.0025372828339622414 0.0 0.0 0.030244411380829915 0.0 46-47 0.0033492133408301586 0.0 0.0 0.04186516676037698 0.0 48-49 0.0035521959675471376 0.0 0.0 0.06779619732347109 0.0 50-51 0.0035521959675471376 0.0 0.0 0.0920526212161501 0.0 52-53 0.0035521959675471376 0.0 0.0 0.11935378450958384 0.0 54-55 0.0035521959675471376 0.0 0.0 0.17025167815886638 0.0 56-57 0.0037551785942641167 0.0 0.0 0.21983018473448857 0.0 58-59 0.0037551785942641167 0.0 0.0 0.28498760791063893 0.0 60-61 0.0038059242509433616 0.0 0.0 0.36293293656995895 0.0 62-63 0.0040089068776603416 0.0 0.0 0.4554930143529015 0.0 64-65 0.004059652534339586 0.0 0.0 0.5344532561458065 0.0 66-67 0.0042626351610565655 0.0 0.0 0.6451295333632394 0.0 68-69 0.004364126474415054 0.0 0.0 0.7916322441962192 0.0 70-71 0.004465617787773544 0.0 0.0 0.8894698702738033 0.0 72-73 0.004668600414490524 0.0 0.0 1.0273965651279906 0.0 74-75 0.0047700917278490134 0.0 0.0 1.1908483252918383 0.0 76-77 0.0047700917278490134 0.0 0.0 1.330297389846403 0.0 78-79 0.0047700917278490134 0.0 0.0 1.4611704384221755 0.0 80-81 0.005074565667924483 0.0 0.0 1.6466458135848154 0.0 82-83 0.005176056981282972 0.0 0.0 1.9013890101146242 0.0 84-85 0.005379039607999951 0.0 0.0 2.1847020113548483 0.0 86-87 0.005632767891396176 0.0 0.0 2.524951639389185 0.0 88-89 0.00568351354807542 0.0 0.0 2.8946337482974833 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4325 0.0 37.677647 1 GTATCAA 6635 0.0 33.438877 1 GTCTACG 85 6.399443E-4 27.946463 1 TATCAAC 8440 0.0 26.284885 2 ATCAACG 8460 0.0 26.051647 3 TCAACGC 8595 0.0 25.641157 4 CAACGCA 8745 0.0 25.255657 5 AACGCAG 8910 0.0 24.840006 6 AGTACTT 6130 0.0 22.239244 12-13 GAGTACT 5945 0.0 22.212198 12-13 ACGCAGA 9990 0.0 22.059519 7 GTACATG 4865 0.0 21.874641 1 CGCAGAG 10130 0.0 21.754648 8 TCGCAAG 110 0.0028781572 21.589514 5 GTAGCAC 310 1.0913936E-11 21.451315 3 TACATGG 4985 0.0 21.155313 2 AGAGTAC 8835 0.0 21.021212 10-11 ACATGGG 4995 0.0 20.825539 3 GCAGAGT 10535 0.0 20.692913 9 GTACTTT 6420 0.0 20.606289 14-15 >>END_MODULE