FastQCFastQC Report
Thu 26 May 2016
SRR936750_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936750_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1105650
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60410.5463754352643241No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46920.424365757699091No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28570.25840003617781393No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11950.1080812191923303No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11230.10156921268032379No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46650.036.3712161
GTATCAA72700.034.190741
TATCAAC88400.028.063372
ATCAACG88350.028.0254573
TCAACGC88550.028.015834
CAACGCA89450.027.5783045
AACGCAG90650.027.3156246
GTACATG55300.025.9551181
GAGTACT53500.025.88564112-13
TACATGG55550.024.7249472
ACGCAGA100450.024.5043077
CGCAGAG100750.024.4313418
ACATGGG57100.023.8040853
GTACTTT58000.023.58844814-15
TACGGGA1252.2478236E-422.8119722
GCAGAGT104750.022.7715539
ATGGGCG1503.017644E-522.1813165
AGTACTT55600.021.78911412-13
AGAGTAC84400.020.5768110-11
CATGGGA26850.020.532194
Produced by FastQC (version 0.11.5)