##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936750_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1105650 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.355599873377653 31.0 31.0 34.0 30.0 34.0 2 31.398820603265047 31.0 31.0 34.0 28.0 34.0 3 31.412820512820513 31.0 31.0 34.0 28.0 34.0 4 35.08315108759553 37.0 35.0 37.0 32.0 37.0 5 35.0389788812011 37.0 35.0 37.0 32.0 37.0 6 35.13925021480577 37.0 35.0 37.0 32.0 37.0 7 34.99049337493782 37.0 35.0 37.0 32.0 37.0 8 34.97432370099037 37.0 35.0 37.0 32.0 37.0 9 36.50499072943517 39.0 37.0 39.0 32.0 39.0 10-11 36.366727716727716 39.0 36.0 39.0 32.0 39.0 12-13 36.265249853027626 39.0 35.0 39.0 32.0 39.0 14-15 37.30678876678877 39.5 37.0 41.0 32.0 41.0 16-17 37.25897345452901 39.0 37.0 41.0 32.0 41.0 18-19 37.24428345317234 39.5 36.5 41.0 31.0 41.0 20-21 37.15252204585538 39.0 36.0 41.0 31.0 41.0 22-23 37.03352733686067 39.0 36.0 41.0 31.0 41.0 24-25 36.88137113914892 39.0 36.0 41.0 30.5 41.0 26-27 36.689118618007505 39.0 36.0 40.5 30.0 41.0 28-29 36.766522859856195 39.0 36.0 41.0 30.0 41.0 30-31 36.67082892416226 39.0 36.0 41.0 30.0 41.0 32-33 36.46281870392981 39.0 35.0 40.5 30.0 41.0 34-35 36.27186813186813 39.0 35.0 40.0 30.0 41.0 36-37 36.01912268801158 38.0 35.0 40.0 29.5 41.0 38-39 35.820832994166324 38.0 35.0 40.0 28.5 41.0 40-41 35.645606204495095 38.0 35.0 40.0 27.5 41.0 42-43 35.33935739157961 38.0 34.5 40.0 27.0 41.0 44-45 35.01834622167955 38.0 34.0 40.0 25.5 41.0 46-47 34.62685569574458 38.0 33.5 40.0 24.0 41.0 48-49 34.32893772893773 38.0 33.0 40.0 23.0 41.0 50-51 33.621332247998915 37.5 32.5 39.5 22.5 40.5 52-53 33.80538732872066 37.5 33.0 39.5 23.0 40.5 54-55 34.16928548817438 38.0 33.5 40.0 23.0 41.0 56-57 34.08688463799575 38.0 33.5 40.0 22.5 41.0 58-59 33.85170352281463 38.0 33.0 40.0 22.0 41.0 60-61 33.55447112558224 37.0 33.0 40.0 20.5 41.0 62-63 33.234806222584 37.0 33.0 40.0 19.5 41.0 64-65 32.92530321530322 36.0 32.0 39.5 18.0 41.0 66-67 32.5692692081581 36.0 32.0 39.0 17.5 41.0 68-69 32.182703839370504 35.5 31.0 39.0 13.5 40.5 70-71 31.784766879211325 35.0 31.0 38.5 12.0 40.0 72-73 31.37697915253471 35.0 31.0 37.5 8.0 40.0 74-75 30.957382987382985 35.0 30.5 37.0 7.0 39.0 76-77 30.550369013702348 35.0 30.0 36.5 6.0 39.0 78-79 30.09332293221182 34.0 30.0 36.0 2.0 39.0 80-81 29.698865825532494 34.0 29.0 36.0 2.0 37.5 82-83 29.290820784154118 34.0 29.0 35.0 2.0 37.0 84-85 28.897845158956272 34.0 29.0 35.0 2.0 36.5 86-87 28.54930176819066 34.0 29.0 35.0 2.0 36.0 88-89 28.160256862479084 34.0 27.5 35.0 2.0 36.0 90-91 27.800393433726768 34.0 27.0 35.0 2.0 35.5 92-93 27.418455207344095 34.0 26.0 35.0 2.0 35.0 94-95 26.98880567991679 33.0 25.5 35.0 2.0 35.0 96-97 26.395414009858456 33.0 25.0 35.0 2.0 35.0 98-99 25.733319314430425 33.0 23.5 35.0 2.0 35.0 100-101 23.730915298693077 31.0 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2483768501497714 1101 2 0.2244102092063187 1101 3 0.32645085576071864 1101 4 0.3526308128321105 1101 5 0.3426305355189996 1101 6 0.1500516971911594 1101 7 0.20765854372934456 1101 8 0.27438251267756897 1101 9 0.3140897895291488 1101 10-11 0.4386026345080154 1101 12-13 0.3892679447639722 1101 14-15 0.6136254156054974 1101 16-17 0.5727656590058245 1101 18-19 0.5284013434573822 1101 20-21 0.44189187828791177 1101 22-23 0.5164719119044179 1101 24-25 0.5070445567317421 1101 26-27 0.5369625629703592 1101 28-29 0.40877524770714047 1101 30-31 0.3840914928752355 1101 32-33 0.4842521968983604 1101 34-35 0.504652542521093 1101 36-37 0.6014722254524685 1101 38-39 0.6239116380309184 1101 40-41 0.4800945861450856 1101 42-43 0.4735596369917303 1101 44-45 0.7276101881311732 1101 46-47 0.8125381821349151 1101 48-49 0.7089353476196649 1101 50-51 0.8852479238937221 1101 52-53 0.904317648961829 1101 54-55 0.9525187726778697 1101 56-57 0.899647877696772 1101 58-59 0.8952636757371053 1101 60-61 0.6309736478652255 1101 62-63 0.8038860771164238 1101 64-65 1.005189314214725 1101 66-67 0.8985642794716284 1101 68-69 0.7613157046656944 1101 70-71 0.8538388711046743 1101 72-73 0.8456961668995362 1101 74-75 0.8804253818985615 1101 76-77 0.7508874927080385 1101 78-79 0.7418259513498562 1101 80-81 0.7299303892606268 1101 82-83 0.7958857472378291 1101 84-85 0.7087047786618328 1101 86-87 0.6183599234193338 1101 88-89 0.4069677042818469 1101 90-91 0.360255492883514 1101 92-93 0.3192281638775789 1101 94-95 0.20773678117607375 1101 96-97 0.11546789972373617 1101 98-99 0.03785918041534231 1101 100-101 0.36828941292801787 1102 1 0.1468720341177132 1102 2 0.08868018289316026 1102 3 0.13598049513596422 1102 4 0.14837564532570013 1102 5 0.16061625123344214 1102 6 0.130081746380327 1102 7 -0.10610890497494552 1102 8 0.04674467323036424 1102 9 0.22680459697408395 1102 10-11 0.29313004375981677 1102 12-13 0.26563935573089026 1102 14-15 0.22584890616253261 1102 16-17 0.11709094320286795 1102 18-19 0.06602461812927629 1102 20-21 -0.15188366323548763 1102 22-23 -0.36976506515175345 1102 24-25 -0.26214398871047706 1102 26-27 -0.21218475967447858 1102 28-29 -0.18481464178679374 1102 30-31 -0.123595823125207 1102 32-33 -0.2064291058156087 1102 34-35 -0.1347050267363059 1102 36-37 -0.262186332301404 1102 38-39 -0.47088843829908456 1102 40-41 -0.7923581013261085 1102 42-43 -0.2556931739409336 1102 44-45 -0.18162852934092655 1102 46-47 -0.1290787090959853 1102 48-49 -0.17783774996433266 1102 50-51 -0.18787727799568188 1102 52-53 -0.1138261008873016 1102 54-55 -0.1379604394677969 1102 56-57 0.12350355997086382 1102 58-59 -0.031728376286842774 1102 60-61 -0.4679432478489076 1102 62-63 -0.4414479068611712 1102 64-65 -0.18258090145200612 1102 66-67 -0.21680135731483574 1102 68-69 -0.2632518640514192 1102 70-71 -0.12029830677755982 1102 72-73 -0.2795528539728096 1102 74-75 -0.4032118344935327 1102 76-77 -0.35284514767765884 1102 78-79 -0.23664430466601516 1102 80-81 -0.21553418338909225 1102 82-83 -0.30562122419704707 1102 84-85 -0.5260642828585382 1102 86-87 -0.6133625480632503 1102 88-89 -0.7916548208846343 1102 90-91 -0.874061077738407 1102 92-93 -0.9210232365594813 1102 94-95 -0.7200611041151106 1102 96-97 -0.8266971822907756 1102 98-99 -0.7518352926720091 1102 100-101 -0.407312030674575 1103 1 0.2617226134045012 1103 2 0.3217590393831138 1103 3 0.28364277905545876 1103 4 0.38198367855824245 1103 5 0.3881897164682968 1103 6 0.14262207550751071 1103 7 0.20575319901224987 1103 8 0.13441497719875883 1103 9 0.4821424461117161 1103 10-11 0.4597558675848532 1103 12-13 0.4770324943776387 1103 14-15 0.4619838041174802 1103 16-17 0.6201926086142393 1103 18-19 0.41261310661251116 1103 20-21 0.2322174748302004 1103 22-23 0.21066766348373278 1103 24-25 0.23473161019919075 1103 26-27 0.35577081145188316 1103 28-29 0.5465332513329244 1103 30-31 0.4010877999837632 1103 32-33 0.39190560488280113 1103 34-35 0.29663551162015267 1103 36-37 0.43230153203403177 1103 38-39 0.16787458207330275 1103 40-41 0.3380757259462115 1103 42-43 0.30533594970088274 1103 44-45 0.2178539821917127 1103 46-47 0.1838843205537799 1103 48-49 0.18283236357367372 1103 50-51 -0.157464721553211 1103 52-53 0.13957463788494096 1103 54-55 0.3184895963599672 1103 56-57 0.2612697200031775 1103 58-59 0.33370097098859475 1103 60-61 0.44928118046320265 1103 62-63 0.2731045756308319 1103 64-65 0.35418604837813206 1103 66-67 0.3109228958257404 1103 68-69 0.23350837799048563 1103 70-71 0.3405752681989149 1103 72-73 0.18221824626315453 1103 74-75 0.20925928051298825 1103 76-77 0.21434656567090116 1103 78-79 0.41088660059853055 1103 80-81 0.7053392451024649 1103 82-83 0.607572885400522 1103 84-85 0.4659379461535309 1103 86-87 0.3773594638581841 1103 88-89 0.3818588455472458 1103 90-91 0.5601969517709797 1103 92-93 0.4895558121919201 1103 94-95 0.3150508713926108 1103 96-97 0.4434734848381332 1103 98-99 0.283038187089268 1103 100-101 0.24793374659267542 1104 1 0.29381305043827766 1104 2 0.3109452054895243 1104 3 0.32189839791465147 1104 4 0.3120046763951976 1104 5 0.40647983698774937 1104 6 0.2979754811647908 1104 7 0.43854520919799 1104 8 0.4387848978407689 1104 9 0.597186678519293 1104 10-11 0.6778632954523687 1104 12-13 0.5296106818621524 1104 14-15 0.4903214991104292 1104 16-17 0.1429740972882385 1104 18-19 0.5209695574949151 1104 20-21 0.34295595148075364 1104 22-23 0.3439749044875242 1104 24-25 0.3649409663848644 1104 26-27 0.45901301512291326 1104 28-29 0.4473811245937256 1104 30-31 0.43196758536855384 1104 32-33 0.3434651139901419 1104 34-35 0.47334702998170286 1104 36-37 0.33209621135385703 1104 38-39 0.20867628859507903 1104 40-41 0.19142316211414112 1104 42-43 0.2989053838576581 1104 44-45 0.235453096257487 1104 46-47 0.3587979360573499 1104 48-49 0.4246847961562281 1104 50-51 0.28164693890262527 1104 52-53 0.47629725127969635 1104 54-55 0.5230532282175915 1104 56-57 0.5245697693955478 1104 58-59 0.6703752695457936 1104 60-61 0.5594115505791919 1104 62-63 0.4816430587465348 1104 64-65 0.27459135077513963 1104 66-67 0.3154771775457377 1104 68-69 0.22336382625311657 1104 70-71 0.1966172124296861 1104 72-73 0.27363669690016224 1104 74-75 0.19540960639483274 1104 76-77 0.15446936182782167 1104 78-79 0.09073250059794091 1104 80-81 0.2092094721439679 1104 82-83 0.07148031139105626 1104 84-85 0.17354106791769652 1104 86-87 0.014687090247537782 1104 88-89 -0.02473953614900637 1104 90-91 0.10794104858418407 1104 92-93 -0.017224427943251186 1104 94-95 0.13375639523841798 1104 96-97 0.12465464705676155 1104 98-99 -0.03810202222172876 1104 100-101 -0.006109492753818557 1105 1 0.12387264471766812 1105 2 0.13980262684560785 1105 3 -0.00794418459764401 1105 4 0.1769782970314253 1105 5 0.32922917415601916 1105 6 0.27484768057586706 1105 7 0.38540091911553986 1105 8 -0.012156202062435284 1105 9 0.23830447828778034 1105 10-11 0.2509454619312095 1105 12-13 0.16084775095776394 1105 14-15 0.2685416370372451 1105 16-17 0.2101773916161349 1105 18-19 0.03449549071990532 1105 20-21 -0.2770887516679821 1105 22-23 -0.4568856608823282 1105 24-25 -0.4148014421354276 1105 26-27 -0.4658800837328627 1105 28-29 -0.2404367147512474 1105 30-31 -0.3009247245080928 1105 32-33 -0.4331998083638524 1105 34-35 -0.35514523347440985 1105 36-37 -0.4457434651987029 1105 38-39 -0.6534955209599858 1105 40-41 -0.8431707665725199 1105 42-43 -0.478778069728385 1105 44-45 -0.33567681451050646 1105 46-47 -0.44880933598358297 1105 48-49 -0.2147904307071471 1105 50-51 -0.35618210173532816 1105 52-53 -0.4849870842965984 1105 54-55 -0.3416544945531683 1105 56-57 -0.16821000893362736 1105 58-59 -0.34023195105847037 1105 60-61 -0.14686844263626853 1105 62-63 -0.39411334735156345 1105 64-65 -0.36826020509933244 1105 66-67 -0.7961276595424032 1105 68-69 -0.3264870286372208 1105 70-71 -0.28254434422271046 1105 72-73 -0.3200681764487072 1105 74-75 -0.4369329261686232 1105 76-77 -0.44828050246012197 1105 78-79 -0.4524766464586705 1105 80-81 -0.348416185320918 1105 82-83 -0.2420249621436703 1105 84-85 -0.5069315288735865 1105 86-87 -0.3069600566959956 1105 88-89 -0.4870096705262661 1105 90-91 -0.26005075725553795 1105 92-93 -0.17586194262502275 1105 94-95 0.09146673192760346 1105 96-97 -0.035205870277184914 1105 98-99 -0.1412782583067269 1105 100-101 0.012646190255118483 1106 1 0.13308017361876523 1106 2 0.2225739730402836 1106 3 0.20175146539112632 1106 4 0.2529642575242832 1106 5 0.3379392482819128 1106 6 0.3521901028011172 1106 7 0.5384385215681462 1106 8 0.4085197620510783 1106 9 0.6487539004709859 1106 10-11 0.6778057632964902 1106 12-13 0.4786995566160286 1106 14-15 0.49983938884936663 1106 16-17 0.32426505443000053 1106 18-19 0.4543473209944864 1106 20-21 0.2944367831471837 1106 22-23 0.26509532711413897 1106 24-25 0.35419902249758195 1106 26-27 0.3770731873143447 1106 28-29 0.10176268153352908 1106 30-31 0.2493897489642407 1106 32-33 0.3803913227867639 1106 34-35 0.26633988955077115 1106 36-37 0.3285356612912196 1106 38-39 0.5183302303762147 1106 40-41 0.26614799731903105 1106 42-43 0.16244831527288994 1106 44-45 -0.024767836426214274 1106 46-47 0.06882620505746928 1106 48-49 0.11862017051566909 1106 50-51 0.46243460474481424 1106 52-53 0.4545185687384432 1106 54-55 0.5626321296571888 1106 56-57 0.47323612799669235 1106 58-59 0.39782887791658084 1106 60-61 0.2713489666397706 1106 62-63 0.39920983516920927 1106 64-65 0.16559184877348088 1106 66-67 0.4280818745629915 1106 68-69 0.5405602336691508 1106 70-71 0.6811465855869336 1106 72-73 0.6997969363812118 1106 74-75 0.41014101407321135 1106 76-77 0.2157052077668311 1106 78-79 0.4029783810654486 1106 80-81 0.4402293837929854 1106 82-83 0.43873958994702633 1106 84-85 0.4570201800630578 1106 86-87 0.6785278541272888 1106 88-89 0.5719043600574629 1106 90-91 0.5610670697799485 1106 92-93 0.42571011703262585 1106 94-95 0.4885079529790666 1106 96-97 0.6119922798064614 1106 98-99 0.6262095848216802 1106 100-101 0.7526699142323388 1107 1 0.20593221673386708 1107 2 0.2236657139337801 1107 3 0.17211849828198567 1107 4 0.3148122456017788 1107 5 0.2844439445006728 1107 6 0.25590895352721077 1107 7 0.3048232995808249 1107 8 0.38412021954249553 1107 9 0.2548780470068692 1107 10-11 0.396881769257746 1107 12-13 0.3848964858781514 1107 14-15 0.5293943744661078 1107 16-17 0.5281693278157817 1107 18-19 0.724171949282713 1107 20-21 0.7783466018835412 1107 22-23 0.6061777102285433 1107 24-25 0.4964112707906594 1107 26-27 0.5252380228600444 1107 28-29 0.5417082677810612 1107 30-31 0.5076211287167709 1107 32-33 0.4462596900276097 1107 34-35 0.3143331658132098 1107 36-37 0.6822337169526804 1107 38-39 0.8171986322754989 1107 40-41 0.6580656488630652 1107 42-43 0.9300374741125239 1107 44-45 0.7825745543130509 1107 46-47 0.9272464733137937 1107 48-49 0.7888624330187 1107 50-51 0.7040039389561628 1107 52-53 0.6321777376290285 1107 54-55 0.8114624072712999 1107 56-57 1.0726105431355037 1107 58-59 1.1132484183456555 1107 60-61 0.9865345625916717 1107 62-63 1.0916334999527706 1107 64-65 0.9517665966333269 1107 66-67 0.9647938282593174 1107 68-69 0.8037650997142656 1107 70-71 0.8520386938598996 1107 72-73 1.011237687776216 1107 74-75 0.8049439808357661 1107 76-77 0.9863269801995074 1107 78-79 0.9464573953793867 1107 80-81 1.1909312172245201 1107 82-83 1.1102988732104642 1107 84-85 1.206179712867275 1107 86-87 1.3868770725794377 1107 88-89 1.5028647454593234 1107 90-91 1.2706726567206488 1107 92-93 1.1643854714151871 1107 94-95 1.3762405977647063 1107 96-97 1.4339172096231145 1107 98-99 1.5884145548421245 1107 100-101 1.5766396920717263 1108 1 0.1645730745531111 1108 2 0.10896066314740338 1108 3 -0.05734435191613585 1108 4 -0.04773538640693431 1108 5 0.01604205905380951 1108 6 0.0982538620188933 1108 7 0.10793589046670604 1108 8 -0.021428091868507693 1108 9 0.2133564390599787 1108 10-11 -0.02032926322274875 1108 12-13 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2316 78-79 -2.3867938199668224 2316 80-81 -2.1961416199370056 2316 82-83 -2.471289079632907 2316 84-85 -2.5158479146097044 2316 86-87 -2.767406201628944 2316 88-89 -2.8092642305443043 2316 90-91 -2.885064852386982 2316 92-93 -2.840337059371592 2316 94-95 -2.5281591346799637 2316 96-97 -2.7988499575153547 2316 98-99 -4.034529509371154 2316 100-101 -4.16823614553174 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2356.0 3 905.0 4 955.0 5 990.0 6 1145.0 7 1465.0 8 1721.0 9 2442.0 10 2914.0 11 3883.0 12 5028.0 13 6124.0 14 6975.0 15 8561.0 16 10827.0 17 13237.0 18 11329.0 19 7722.0 20 7509.0 21 7920.0 22 8848.0 23 9955.0 24 11369.0 25 13254.0 26 15184.0 27 18149.0 28 21110.0 29 25647.0 30 30870.0 31 38003.0 32 47188.0 33 59907.0 34 78159.0 35 106455.0 36 145428.0 37 189908.0 38 155340.0 39 26859.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.23328111035932 18.691724045604044 14.779704680891095 22.295290163145538 2 15.264971644099532 20.137121356018053 40.951709042230846 23.646197957651573 3 18.997827341487287 25.117180645429727 30.082998811459916 25.80199320162307 4 12.39513513904773 16.794030574576198 37.972479986102464 32.83835430027361 5 13.521452007839887 37.42016689620475 34.20060120276749 14.857779893187878 6 31.068702773687484 36.33433763017923 17.825831576794318 14.771128019338974 7 27.68283988549549 33.50143492532236 21.03857470116278 17.77715048801937 8 26.197289626701675 34.297829837629116 20.06561061205509 19.43926992361412 9 26.604152664187453 16.70075507097052 20.773995236367877 35.92109702847415 10-11 24.746219764906023 26.701445131165784 27.36460378792678 21.18773131600141 12-13 25.92881477091241 24.326898280316158 27.95284781634989 21.791439132421537 14-15 22.90021091406801 25.416253364651563 26.869900441141947 24.81363528013848 16-17 21.568510376522102 28.680996580361505 27.39347554687076 22.357017496245636 18-19 21.414819593108522 26.882983641648885 30.50473759778957 21.197459167453026 20-21 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26.87001842828233 29.400820793433653 21.91007450452793 46-47 21.946174360124655 27.030897894449797 29.008014015595695 22.014913729829857 48-49 22.135832148503496 27.145672567300146 28.66501333159679 22.053481952599572 50-51 21.952236475978275 27.480148276849174 28.462841924704772 22.10477332246778 52-53 22.224568769228057 27.521619762608836 28.117471861409214 22.136339606753893 54-55 21.912170785159134 27.397675337795974 28.24602056939843 22.444133307646457 56-57 21.98242451595514 27.670398170818295 28.08319412172777 22.26398319149879 58-59 22.132242908871863 27.383799834392235 28.188277303912855 22.29567995282304 60-61 22.161448134705324 27.232388754778892 28.526251240228532 22.079911870287244 62-63 21.99408940955146 27.485059592047158 28.407554883246622 22.113296115154764 64-65 21.916017857918117 27.386497662841347 28.43613876789048 22.261345711350053 66-67 21.875552568826052 27.604269596153873 28.115741999029513 22.40443583599056 68-69 21.930227614994642 27.395881855471487 28.32203474147405 22.351855788059822 70-71 21.95790969484461 27.29027096431057 28.267792138131963 22.484027202712852 72-73 21.95925610532071 27.30578995281805 28.2506499784961 22.484303963365136 74-75 21.790669488612725 27.692652086612036 28.03336589424938 22.483312530525858 76-77 21.902151006401827 27.545218890362165 28.060901465419462 22.49172863781654 78-79 21.895454551623335 27.635904513872394 28.06489452129592 22.40374641320835 80-81 21.865160621180436 27.75577239788079 27.854468675239946 22.524598305698827 82-83 21.931888998532028 27.727619307764872 27.92628845512013 22.41420323858297 84-85 21.946259006731417 27.6185777007531 27.734039339268175 22.701123953247308 86-87 21.905246034053974 27.63858594017467 27.773177329129766 22.682990696641593 88-89 21.821664917237136 27.53960863731273 27.769303232593025 22.86942321285711 90-91 21.773717207112924 27.510341128338535 28.01620389005524 22.6997377744933 92-93 21.89961144085294 27.46051653807414 27.9366835384527 22.70318848262022 94-95 21.78640557163531 27.45577062228654 27.828690303907383 22.929133502170767 96-97 21.734928534764826 27.39738107782373 27.97448461592382 22.893205771487622 98-99 21.748472126917843 27.413781639106677 27.66939590266303 23.168350331312453 100-101 21.723130101163655 27.418517110954266 27.781367066615992 23.076985721266084 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 428.0 1 305.5 2 322.0 3 457.5 4 396.0 5 384.5 6 574.5 7 1019.0 8 1660.5 9 1985.5 10 1740.5 11 1479.0 12 1547.0 13 1678.5 14 1819.0 15 1908.5 16 1896.5 17 2006.0 18 2123.0 19 2277.5 20 2504.5 21 2800.5 22 3307.5 23 3997.5 24 4826.0 25 5887.0 26 7365.0 27 9297.0 28 10916.0 29 12483.0 30 14625.0 31 16591.0 32 18595.5 33 20372.5 34 22175.0 35 24324.0 36 26775.5 37 29075.0 38 30193.0 39 30811.0 40 31939.0 41 33646.0 42 35211.0 43 36564.0 44 37709.0 45 38908.5 46 39350.0 47 40104.0 48 43117.0 49 43220.0 50 39788.0 51 37559.5 52 36551.5 53 36085.0 54 35031.5 55 32114.0 56 29003.5 57 26117.5 58 23931.5 59 21758.0 60 18226.5 61 14893.5 62 12055.0 63 9407.5 64 6996.5 65 5203.5 66 4023.0 67 3199.0 68 2515.5 69 1910.0 70 1439.5 71 1138.0 72 961.0 73 857.5 74 672.0 75 509.5 76 376.5 77 241.5 78 165.0 79 117.0 80 89.0 81 61.0 82 44.5 83 36.0 84 27.0 85 14.5 86 8.5 87 6.0 88 5.0 89 4.0 90 3.0 91 1.0 92 3.0 93 4.5 94 3.5 95 2.5 96 2.0 97 1.5 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012119567675123231 2 0.00542667209333876 3 0.008320897209786099 4 0.03780581558359336 5 0.048116492560937 6 0.06611495500384389 7 0.03310269976936643 8 0.030841586397141952 9 0.017274906163795054 10-11 0.05064893953782843 12-13 0.04567449011893456 14-15 0.02021435354768688 16-17 0.02442002442002442 18-19 0.03102247546691991 20-21 0.025324469768914216 22-23 0.015646904535793423 24-25 0.00411522633744856 26-27 0.003120336453669787 28-29 0.0020350020350020353 30-31 0.00669289558178447 32-33 0.001085334418667752 34-35 0.002442002442002442 36-37 0.005019671686338353 38-39 0.0053814498258942705 40-41 0.003346447790892235 42-43 5.878894767783657E-4 44-45 0.0011305566861122417 46-47 0.0022158911047799935 48-49 5.42667209333876E-4 50-51 0.0014018902907791796 52-53 5.878894767783657E-4 54-55 0.002442002442002442 56-57 0.0017184461628906075 58-59 0.0023063356396689732 60-61 6.331117442228553E-4 62-63 6.78334011667345E-4 64-65 0.0030751141862252972 66-67 0.0016732238954461176 68-69 0.004974449418893864 70-71 0.008908786686564463 72-73 0.0033012255234477457 74-75 0.00271333604666938 76-77 0.0025324469768914213 78-79 0.034323700990367655 80-81 0.02157102157102157 82-83 0.003889115000226111 84-85 0.007959119070230182 86-87 0.0073712295934518154 88-89 0.004657893546782436 90-91 0.010220232442454664 92-93 0.014064125175236286 94-95 0.004522226744448967 96-97 0.003843892732781621 98-99 0.024736580292135844 100-101 0.04612671279337946 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1105650.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.695159671180924 #Duplication Level Percentage of deduplicated Percentage of total 1 79.3870152606562 49.771815975853166 2 12.268046536070589 15.382942728648471 3 3.773277681617218 7.0969874019808445 4 1.6418289131016413 4.117389034386753 5 0.8446123589867562 2.647655335346373 6 0.4728729045018751 1.7788105351152088 7 0.3199464983501902 1.4041367760210277 8 0.2202574162534887 1.104725910461939 9 0.16151654726571552 0.9113675148325686 >10 0.8378333794055152 9.504064194566006 >50 0.04796717488670711 2.028797578216763 >100 0.023381997830537186 2.4987896612849045 >500 7.216655367925613E-4 0.3130044676519982 >1k 5.77332429434049E-4 0.8928635681764284 >5k 1.4433310735851226E-4 0.5466493174575661 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6041 0.5463754352643241 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4692 0.424365757699091 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2857 0.25840003617781393 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1195 0.1080812191923303 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1123 0.10156921268032379 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 9.044453488897933E-5 0.0 0.0 0.0 0.0 6 9.044453488897933E-5 0.0 0.0 0.0 0.0 7 2.71333604666938E-4 0.0 0.0 0.0 0.0 8 2.71333604666938E-4 0.0 0.0 0.0 0.0 9 2.71333604666938E-4 0.0 0.0 9.044453488897933E-5 0.0 10-11 2.71333604666938E-4 0.0 0.0 9.044453488897933E-5 0.0 12-13 2.71333604666938E-4 0.0 0.0 9.044453488897933E-5 0.0 14-15 3.617781395559173E-4 0.0 0.0 9.044453488897933E-5 0.0 16-17 3.617781395559173E-4 0.0 0.0 9.044453488897933E-5 0.0 18-19 5.878894767783657E-4 0.0 0.0 9.044453488897933E-5 0.0 20-21 8.14000814000814E-4 0.0 0.0 9.044453488897933E-5 0.0 22-23 8.14000814000814E-4 0.0 0.0 9.044453488897933E-5 0.0 24-25 0.00135666802333469 0.0 0.0 3.1655587211142766E-4 0.0 26-27 0.00135666802333469 0.0 0.0 3.617781395559173E-4 0.0 28-29 0.0017184461628906075 0.0 0.0 9.044453488897934E-4 0.0 30-31 0.001899335232668566 0.0 0.0 0.0024872247094469314 0.0 32-33 0.0019897797675575454 0.0 0.0 0.0038438927327816216 0.0 34-35 0.002306335639668973 0.0 0.0 0.00547189436078325 0.0 36-37 0.0026228915117804006 0.0 0.0 0.007145118256229367 0.0 38-39 0.0026228915117804006 0.0 0.0 0.009632342965676298 0.0 40-41 0.0026228915117804006 0.0 0.0 0.012978790756568534 0.0 42-43 0.00271333604666938 0.0 0.0 0.01917424139646362 0.0 44-45 0.0030298919187808073 0.0 0.0 0.02934925157147379 0.0 46-47 0.0036177813955591736 0.0 0.0 0.039750373083706415 0.0 48-49 0.0038891150002261115 0.0 0.0 0.0612761723872835 0.0 50-51 0.0038891150002261115 0.0 0.0 0.07990774657441324 0.0 52-53 0.0038891150002261115 0.0 0.0 0.10224754669199114 0.0 54-55 0.0038891150002261115 0.0 0.0 0.14548003436892326 0.0 56-57 0.00407000407000407 0.0 0.0 0.18658707547596437 0.0 58-59 0.00407000407000407 0.0 0.0 0.2423913535024646 0.0 60-61 0.00407000407000407 0.0 0.0 0.30140641251752365 0.0 62-63 0.00407000407000407 0.0 0.0 0.385112829557274 0.0 64-65 0.004250893139782028 0.0 0.0 0.4516800072355628 0.0 66-67 0.004431782209559987 0.0 0.0 0.5482747704969927 0.0 68-69 0.004431782209559987 0.0 0.0 0.6743996743996744 0.0 70-71 0.004748338081671415 0.0 0.0 0.759507981730204 0.0 72-73 0.004884004884004884 0.0 0.0 0.8770858770858772 0.0 74-75 0.0049292271514493734 0.0 0.0 1.0148329037217927 0.0 76-77 0.004974449418893864 0.0 0.0 1.1327273549495773 0.0 78-79 0.004974449418893864 0.0 0.0 1.243928910595577 0.0 80-81 0.00547189436078325 0.0 0.0 1.402387735721069 0.0 82-83 0.005698005698005698 0.0 0.0 1.6203138425360648 0.0 84-85 0.005743227965450188 0.0 0.0 1.8706190928413151 0.0 86-87 0.0058336725003391675 0.0 0.0 2.1673223895446116 0.0 88-89 0.005924117035228146 0.0 0.0 2.5055849500293945 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4665 0.0 36.371216 1 GTATCAA 7270 0.0 34.19074 1 TATCAAC 8840 0.0 28.06337 2 ATCAACG 8835 0.0 28.025457 3 TCAACGC 8855 0.0 28.01583 4 CAACGCA 8945 0.0 27.578304 5 AACGCAG 9065 0.0 27.315624 6 GTACATG 5530 0.0 25.955118 1 GAGTACT 5350 0.0 25.885641 12-13 TACATGG 5555 0.0 24.724947 2 ACGCAGA 10045 0.0 24.504307 7 CGCAGAG 10075 0.0 24.431341 8 ACATGGG 5710 0.0 23.804085 3 GTACTTT 5800 0.0 23.588448 14-15 TACGGGA 125 2.2478236E-4 22.811972 2 GCAGAGT 10475 0.0 22.771553 9 ATGGGCG 150 3.017644E-5 22.181316 5 AGTACTT 5560 0.0 21.789114 12-13 AGAGTAC 8440 0.0 20.57681 10-11 CATGGGA 2685 0.0 20.53219 4 >>END_MODULE