FastQCFastQC Report
Thu 26 May 2016
SRR936750_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936750_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1105650
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65030.5881608103830326No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56810.5138154027042916No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36320.3284945507167729No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14880.13458146791480124No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13260.11992945326278659No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12180.11016144349477683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46050.038.0720141
GTATCAA73050.036.0989651
TCAACGC94200.027.9811674
ATCAACG94300.027.8532873
TATCAAC94900.027.6809432
CAACGCA95500.027.5455575
AACGCAG97750.026.9115146
GTACATG58350.025.0250761
TACATGG59050.024.2945582
ACGCAGA108950.023.8835287
CGCAGAG110100.023.5909358
ACATGGG58150.023.115313
GAGTACT64650.023.02577212-13
GCAGAGT117450.021.8720469
GTGTAGG5400.021.3481431
AGAGTAC98650.021.2750410-11
AGTACTT68100.021.09227612-13
GTACTTT70250.021.08887514-15
TAAGGCG2751.2641976E-920.7201885
CATGGGA28300.019.634654
Produced by FastQC (version 0.11.5)