##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936750_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1105650 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.418562836340612 31.0 31.0 34.0 30.0 34.0 2 31.669981458870346 31.0 31.0 34.0 30.0 34.0 3 31.91278343056121 31.0 31.0 34.0 30.0 34.0 4 35.74063582508027 37.0 35.0 37.0 35.0 37.0 5 35.54821236376792 37.0 35.0 37.0 33.0 37.0 6 35.646682946682944 37.0 35.0 37.0 33.0 37.0 7 35.57972776194998 37.0 35.0 37.0 33.0 37.0 8 35.55719441052774 37.0 35.0 37.0 33.0 37.0 9 37.176834441278885 39.0 37.0 39.0 33.0 39.0 10-11 37.06300818523041 39.0 37.0 39.0 33.0 39.0 12-13 36.933737168181615 39.0 37.0 39.0 33.0 39.0 14-15 38.02615746393524 40.0 37.0 41.0 33.0 41.0 16-17 37.95229457785013 40.0 37.0 41.0 33.0 41.0 18-19 38.02685705241261 40.0 37.0 41.0 33.0 41.0 20-21 38.101377922489036 40.0 37.0 41.0 33.0 41.0 22-23 38.00152670374892 40.0 37.0 41.0 33.0 41.0 24-25 37.89686293130738 40.0 37.0 41.0 33.0 41.0 26-27 37.674339076561296 39.5 37.0 41.0 32.0 41.0 28-29 37.57016641794419 39.0 36.5 41.0 32.0 41.0 30-31 37.516570795459685 39.0 36.5 41.0 32.0 41.0 32-33 37.29596119929453 39.0 36.0 41.0 32.0 41.0 34-35 37.113208972097866 39.0 36.0 41.0 31.0 41.0 36-37 36.87055713833492 39.0 35.0 41.0 31.0 41.0 38-39 36.6778623434179 39.0 35.0 41.0 30.5 41.0 40-41 36.405381902048575 39.0 35.0 40.0 30.0 41.0 42-43 36.122260661149554 39.0 35.0 40.0 30.0 41.0 44-45 35.78651019762131 38.5 35.0 40.0 29.0 41.0 46-47 35.31547460769683 38.0 35.0 40.0 26.5 41.0 48-49 34.77406050739384 38.0 34.0 40.0 24.0 41.0 50-51 34.58351196128974 38.0 34.0 40.0 24.0 41.0 52-53 34.31209605209605 38.0 33.5 40.0 23.5 41.0 54-55 34.032368742368746 38.0 33.0 40.0 23.0 41.0 56-57 33.75479989146656 37.5 33.0 40.0 22.5 41.0 58-59 33.370363134807576 37.0 32.5 40.0 21.0 41.0 60-61 33.001818387373945 36.5 32.0 39.5 20.0 41.0 62-63 32.707210690544024 36.0 32.0 39.0 19.0 40.0 64-65 32.28190747524081 36.0 31.0 39.0 18.0 40.0 66-67 31.880159182381405 35.0 31.0 38.5 16.0 40.0 68-69 31.429720526387193 35.0 30.5 38.0 13.0 40.0 70-71 30.95615022837245 35.0 30.0 37.5 9.0 39.5 72-73 30.5360679238457 34.0 29.0 37.0 7.5 39.0 74-75 30.153835300501967 34.0 29.0 36.0 7.0 39.0 76-77 28.752884728440286 32.5 27.0 35.0 5.5 37.0 78-79 29.565910550354992 34.0 29.0 35.0 2.0 37.5 80-81 29.40983855650522 34.0 29.0 35.0 2.0 37.0 82-83 29.05829692940804 34.0 29.0 35.0 2.0 37.0 84-85 28.601069506625063 34.0 28.5 35.0 2.0 36.0 86-87 28.19899968344413 33.0 27.5 35.0 2.0 36.0 88-89 27.7688974811197 33.0 27.0 35.0 2.0 35.5 90-91 27.29331162664496 33.0 26.0 35.0 2.0 35.0 92-93 26.79427214760548 32.5 25.0 34.5 2.0 35.0 94-95 26.24493239271017 32.0 24.5 34.0 2.0 35.0 96-97 25.555802469135806 32.0 23.0 34.0 2.0 35.0 98-99 24.662469588025143 32.0 15.5 34.0 2.0 35.0 100-101 21.9515271559716 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.37210640658828 1101 2 0.3214227867662771 1101 3 0.21332379180372385 1101 4 0.053674185099751526 1101 5 0.23591542431466195 1101 6 0.17409973791398414 1101 7 0.13272765230223627 1101 8 0.0791412264652891 1101 9 0.15427419093307293 1101 10-11 0.16614192936809502 1101 12-13 0.14346948540519122 1101 14-15 0.31964635946574305 1101 16-17 0.3172556627318599 1101 18-19 0.2463132967891397 1101 20-21 0.11954858221788811 1101 22-23 0.16110036066544353 1101 24-25 -0.0060867361528451625 1101 26-27 0.1154601335962937 1101 28-29 0.12723070997791552 1101 30-31 -0.0502551885894178 1101 32-33 0.1774606523981035 1101 34-35 0.34936816043369134 1101 36-37 0.3086104795482143 1101 38-39 0.3463486295720699 1101 40-41 0.2535787599778132 1101 42-43 0.3875443157756706 1101 44-45 0.34530142899938454 1101 46-47 0.6185750753576897 1101 48-49 0.720385409352609 1101 50-51 0.7423172593630696 1101 52-53 0.738429613157237 1101 54-55 0.7905474678619413 1101 56-57 0.7907769076329814 1101 58-59 0.8358688529401448 1101 60-61 0.9198657249465398 1101 62-63 0.805063481760385 1101 64-65 0.8594506974425968 1101 66-67 0.6383120620288594 1101 68-69 0.48119934237758955 1101 70-71 0.62821262133318 1101 72-73 0.6249144383077763 1101 74-75 0.6408277194635446 1101 76-77 0.8607156001287954 1101 78-79 1.0137472918235026 1101 80-81 0.8111867523729401 1101 82-83 0.7202855295057766 1101 84-85 0.6662788490539704 1101 86-87 0.7433138655539508 1101 88-89 0.6777305083331342 1101 90-91 0.7230577287578193 1101 92-93 0.555780130978242 1101 94-95 0.2979462611290984 1101 96-97 0.24641881885478156 1101 98-99 0.15874022198707038 1101 100-101 0.16274887226464685 1102 1 0.33962391133304237 1102 2 0.4079487027549966 1102 3 0.15500405187750133 1102 4 0.1140622495710133 1102 5 0.03804398518381191 1102 6 0.01802529547358489 1102 7 0.012660219806576833 1102 8 -0.14277301264303333 1102 9 0.12144309519525365 1102 10-11 0.06522726262785739 1102 12-13 -9.31870142004243E-4 1102 14-15 0.11434955762370436 1102 16-17 0.08730464497938328 1102 18-19 0.06191140095429404 1102 20-21 0.12287753002495094 1102 22-23 -0.01680112765457409 1102 24-25 0.03116451599249359 1102 26-27 -0.10673588850721671 1102 28-29 -0.09501287468113873 1102 30-31 0.0380622982223926 1102 32-33 0.3108384157682096 1102 34-35 0.3265183040697437 1102 36-37 0.40073340188359197 1102 38-39 0.16023390696378925 1102 40-41 0.414074652067427 1102 42-43 0.29544840782228476 1102 44-45 0.4094243754752753 1102 46-47 0.6035224605379454 1102 48-49 0.5280622644647011 1102 50-51 0.5331753625856024 1102 52-53 0.4517209477481785 1102 54-55 0.5044793881706653 1102 56-57 0.4637356262086385 1102 58-59 0.43542403527572304 1102 60-61 0.46433628329358356 1102 62-63 0.3260093989676278 1102 64-65 0.30342200701936406 1102 66-67 0.25445005149209976 1102 68-69 0.41156934169935866 1102 70-71 0.5013437433956511 1102 72-73 0.36474998969430317 1102 74-75 -0.07414425455207052 1102 76-77 0.1566061378529433 1102 78-79 0.516899065518853 1102 80-81 0.4919138300950401 1102 82-83 0.5304643762100802 1102 84-85 0.5810698841876096 1102 86-87 0.403828150414288 1102 88-89 0.2695999552970072 1102 90-91 0.35831945788519093 1102 92-93 0.4428324250740481 1102 94-95 -0.009631445453329945 1102 96-97 0.028311595898767195 1102 98-99 -0.4377676518431244 1102 100-101 -0.35626091843711905 1103 1 0.4644000640777399 1103 2 0.4362611843680888 1103 3 0.30658816412400824 1103 4 0.16577996826383412 1103 5 0.0367393539412717 1103 6 0.14166802121598465 1103 7 0.09985963582006008 1103 8 0.21524618176319876 1103 9 0.025268426586848136 1103 10-11 0.11922419498966974 1103 12-13 0.1939678304184156 1103 14-15 0.22586531634840412 1103 16-17 0.12438730031138334 1103 18-19 0.23736065186708544 1103 20-21 0.11850480282990361 1103 22-23 0.12636010218391647 1103 24-25 0.05366963380908629 1103 26-27 0.09907190253694154 1103 28-29 0.23217124586618354 1103 30-31 0.21086322400703494 1103 32-33 0.17956565720097473 1103 34-35 0.3123333766347045 1103 36-37 0.15958368453843974 1103 38-39 0.22716408371004349 1103 40-41 0.308380905630365 1103 42-43 0.3405781202113687 1103 44-45 0.4803303968596353 1103 46-47 0.5528479890738183 1103 48-49 0.7086943346505095 1103 50-51 0.4051689123666691 1103 52-53 0.4721526937140439 1103 54-55 0.5692566944840394 1103 56-57 0.6362631134919141 1103 58-59 0.48426729275835356 1103 60-61 0.4171031391292317 1103 62-63 0.31901657550758244 1103 64-65 0.03150237108319942 1103 66-67 0.07465538671671368 1103 68-69 0.202924626203572 1103 70-71 0.0795732474099502 1103 72-73 0.019422339568631486 1103 74-75 0.21524874249259796 1103 76-77 0.219884262650762 1103 78-79 0.1811028528862373 1103 80-81 0.16452829279448977 1103 82-83 0.272767204467506 1103 84-85 0.45000388296934446 1103 86-87 0.4561525607820016 1103 88-89 0.41055429839152424 1103 90-91 -0.00929770257804563 1103 92-93 0.1642486411978865 1103 94-95 0.3917866505602028 1103 96-97 0.10761852695442187 1103 98-99 0.061788773534413366 1103 100-101 0.2123693988125801 1104 1 0.3687797867227651 1104 2 0.3239281442838049 1104 3 0.14790659609549905 1104 4 0.12188020312955672 1104 5 0.01587603383600822 1104 6 0.1757553556724929 1104 7 0.03698430513280471 1104 8 0.07128047904126333 1104 9 0.05494694521917154 1104 10-11 0.01101859762884061 1104 12-13 0.025215399469530553 1104 14-15 0.18108771580227057 1104 16-17 0.16976362249556587 1104 18-19 0.10147882092243066 1104 20-21 0.017341353734458664 1104 22-23 0.17083947157448165 1104 24-25 0.1260831476429516 1104 26-27 0.0694010308573283 1104 28-29 -0.008849580609513907 1104 30-31 0.09619317089894253 1104 32-33 -0.019779624611828694 1104 34-35 0.09105889378594867 1104 36-37 0.1657561657277995 1104 38-39 0.0845317314691485 1104 40-41 0.2611241282578334 1104 42-43 0.25935635962564874 1104 44-45 0.2695232019113476 1104 46-47 0.40445522211782503 1104 48-49 0.3516240490750633 1104 50-51 0.34187265169178005 1104 52-53 0.30857585939256893 1104 54-55 0.4218651569494938 1104 56-57 0.2169488922106595 1104 58-59 0.46434141953718466 1104 60-61 0.2301490490001683 1104 62-63 0.0812719468835823 1104 64-65 0.18509054722588303 1104 66-67 0.4044606362107146 1104 68-69 0.47972571818841914 1104 70-71 0.3422683185665818 1104 72-73 0.3896674556858706 1104 74-75 0.25269795542999063 1104 76-77 0.5632988286357978 1104 78-79 0.6291369430014022 1104 80-81 0.5755528697529009 1104 82-83 0.5542327638090754 1104 84-85 0.5030415827937773 1104 86-87 0.5990297893038843 1104 88-89 0.5654899023600173 1104 90-91 0.45976376953682063 1104 92-93 0.5382362454875498 1104 94-95 0.5822023771441067 1104 96-97 0.37067230971075205 1104 98-99 0.4257020390161017 1104 100-101 0.28067284936095405 1105 1 0.32862602081405967 1105 2 0.4136672923138889 1105 3 0.16151351020517524 1105 4 0.13589903575503115 1105 5 -0.030393595526604145 1105 6 -0.022746434196349696 1105 7 0.12964684184520792 1105 8 0.01659096315392361 1105 9 -0.03528701514568411 1105 10-11 0.020707397228569846 1105 12-13 0.0045295657681379 1105 14-15 0.07956234200428725 1105 16-17 0.13333178905858745 1105 18-19 0.04557225373402218 1105 20-21 0.06990219886031923 1105 22-23 0.1819017579900617 1105 24-25 -0.06111277551151062 1105 26-27 -0.0876175037754976 1105 28-29 -0.10142454744136131 1105 30-31 0.05776720974511562 1105 32-33 0.31364545871771554 1105 34-35 0.22300332347010254 1105 36-37 0.023844498231490263 1105 38-39 0.14405076877860523 1105 40-41 0.02098413547597744 1105 42-43 0.009097369578434211 1105 44-45 0.06840579323164064 1105 46-47 0.4385218185868922 1105 48-49 0.3269901315599384 1105 50-51 0.3912325201159135 1105 52-53 0.02224020404042193 1105 54-55 0.25466917428153124 1105 56-57 0.2489335785340714 1105 58-59 0.2672556261349328 1105 60-61 0.21956818437937642 1105 62-63 0.2434097062083893 1105 64-65 0.28269631867615175 1105 66-67 0.2764827820383644 1105 68-69 0.1803204707121111 1105 70-71 0.15335458042518013 1105 72-73 0.05671444202794618 1105 74-75 -0.017228762183076896 1105 76-77 0.05529319865765103 1105 78-79 0.3286107160347278 1105 80-81 0.189428359836036 1105 82-83 0.37674434450068617 1105 84-85 0.4603153453697253 1105 86-87 0.12092778569651585 1105 88-89 0.3943913212046297 1105 90-91 -0.07900503195033792 1105 92-93 0.12669840360899798 1105 94-95 0.2266229642278894 1105 96-97 0.42482535380558417 1105 98-99 0.7589360414655069 1105 100-101 0.7923130544785231 1106 1 0.4373291203162992 1106 2 0.37803414520984546 1106 3 0.0914790576156932 1106 4 0.020450552068986383 1106 5 -0.05408628950575434 1106 6 -0.07396273676372545 1106 7 0.04610982556869203 1106 8 0.13542008795148064 1106 9 0.055591305364941945 1106 10-11 -0.04365812228345334 1106 12-13 0.06025501381078158 1106 14-15 0.09402545496345027 1106 16-17 0.10091466143425976 1106 18-19 -0.0025034168298887494 1106 20-21 -0.014111245752445711 1106 22-23 0.048617469252945966 1106 24-25 0.08967910069302576 1106 26-27 0.17133719501819655 1106 28-29 0.09098044638917457 1106 30-31 -0.12193217371866893 1106 32-33 0.08950592726883144 1106 34-35 -0.10600300805222673 1106 36-37 -0.050248313495721675 1106 38-39 -0.01531348401868371 1106 40-41 0.13466844964995062 1106 42-43 -0.21789611632821249 1106 44-45 -0.14348173480788518 1106 46-47 -0.1397529467118943 1106 48-49 -0.2490963455679136 1106 50-51 -0.2853475142033446 1106 52-53 0.02570520732771797 1106 54-55 -0.05576311904653153 1106 56-57 0.006474633938225338 1106 58-59 0.1775311955668073 1106 60-61 0.2833465021206578 1106 62-63 -0.15879247992585022 1106 64-65 0.23621891389258565 1106 66-67 0.27761051714216123 1106 68-69 0.20812101288653295 1106 70-71 0.20196257888255786 1106 72-73 0.45532085068140304 1106 74-75 0.4930608590640908 1106 76-77 0.3997162690716074 1106 78-79 0.4095981483783113 1106 80-81 0.18485844282379915 1106 82-83 0.24385391686394087 1106 84-85 0.4206838414049585 1106 86-87 0.519084946532363 1106 88-89 0.3742431207088188 1106 90-91 0.08701178099538609 1106 92-93 0.5527009801170308 1106 94-95 0.6283461578260052 1106 96-97 0.5214613287092504 1106 98-99 0.360565282780307 1106 100-101 0.27359593393138226 1107 1 0.503977302481065 1107 2 0.44807885459881547 1107 3 0.1889212646652183 1107 4 0.06971752604049186 1107 5 0.05753121993866728 1107 6 -0.003883368933692566 1107 7 -0.12108249412077043 1107 8 0.16118863888205937 1107 9 0.17741572570251662 1107 10-11 0.2056833673363485 1107 12-13 0.16098977276132587 1107 14-15 0.2752284494527544 1107 16-17 0.23179359949961764 1107 18-19 0.007218275888369874 1107 20-21 0.06082896843671648 1107 22-23 0.08721830798722863 1107 24-25 0.047603753242157154 1107 26-27 0.1503948320056807 1107 28-29 0.44327483518757305 1107 30-31 0.30559702601792793 1107 32-33 0.19768358643772643 1107 34-35 0.18232056315354583 1107 36-37 0.16353361559310287 1107 38-39 0.20131910184759505 1107 40-41 0.3555900175978195 1107 42-43 0.23759378073621207 1107 44-45 0.2735892803378519 1107 46-47 0.19960517895960805 1107 48-49 0.38757921676529605 1107 50-51 0.16458836768069318 1107 52-53 0.17732722236948462 1107 54-55 0.06561074155722224 1107 56-57 0.23201443915778697 1107 58-59 0.10409862356389255 1107 60-61 -0.04269411757226038 1107 62-63 -0.032393145367919374 1107 64-65 0.11364933707214675 1107 66-67 0.47183642086100974 1107 68-69 0.29066009027883055 1107 70-71 0.1074056870515534 1107 72-73 0.40752686045508213 1107 74-75 0.3876385459597351 1107 76-77 0.15438649400488913 1107 78-79 0.15501456954768855 1107 80-81 0.1867299986412192 1107 82-83 0.1908611604781285 1107 84-85 0.2960434227309925 1107 86-87 0.7388281274840836 1107 88-89 0.9119168461446456 1107 90-91 0.6334194335791707 1107 92-93 0.8692711807894042 1107 94-95 0.764449855881665 1107 96-97 0.9009365999115495 1107 98-99 1.1969341179101 1107 100-101 1.1973040405407467 1108 1 0.2628717756910923 1108 2 0.26644212805191003 1108 3 -0.06210944347452596 1108 4 0.03505323939180727 1108 5 -0.2071824816222687 1108 6 -0.10265200502303884 1108 7 -0.07689602562013675 1108 8 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-0.6421554953105932 2316 80-81 -0.666299707167358 2316 82-83 -0.6210991003883244 2316 84-85 -0.6076426297528528 2316 86-87 -0.7309255782767181 2316 88-89 -0.8882214106845865 2316 90-91 -0.6627398335103258 2316 92-93 -0.9323422136994353 2316 94-95 -0.9029409421260048 2316 96-97 -0.9747307953656197 2316 98-99 -1.2778138545221402 2316 100-101 -1.975134774093572 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 6.0 4 9.0 5 37.0 6 96.0 7 231.0 8 612.0 9 1172.0 10 1825.0 11 2945.0 12 4030.0 13 5579.0 14 7238.0 15 9519.0 16 12003.0 17 15014.0 18 12518.0 19 8334.0 20 7205.0 21 7485.0 22 8013.0 23 8988.0 24 10644.0 25 12654.0 26 15086.0 27 18936.0 28 23421.0 29 28527.0 30 34909.0 31 42289.0 32 52155.0 33 65489.0 34 84724.0 35 112934.0 36 155754.0 37 200570.0 38 126492.0 39 8204.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.507867610139684 19.991000606361585 15.49359021846394 22.007541565034792 2 15.525708088394252 21.227862768385243 39.630970008511625 23.615459134708882 3 19.144598430153785 25.449399684872997 30.4851906590713 24.92081122590192 4 12.873621025895224 17.6249081889986 37.25500483025367 32.2464659548525 5 13.30520508298286 38.101478768145434 33.83150183150183 14.761814317369874 6 30.977795866684755 37.14249536471759 17.109121331343555 14.770587437254104 7 27.47370325148103 34.03789626011849 20.600189933523268 17.888210554877222 8 26.41830597386153 34.786415230859674 19.50653461764573 19.288744177633067 9 26.458029007267648 16.680490419099392 20.552919947358188 36.308560626274776 10-11 24.89926712594526 27.196790657415974 26.866214985433867 21.037727231204897 12-13 26.1095637537608 24.632150271989698 27.842347726243922 21.41593824800558 14-15 22.840410618188393 25.76479898702121 26.852846741735632 24.541943653054766 16-17 21.37498304164971 28.990277212499432 27.53787364898476 22.096866096866098 18-19 21.260173318940588 26.896724504465475 30.93937093084201 20.90373124575193 20-21 22.341292452403565 25.93736715958938 31.05851761407317 20.662822773933883 22-23 21.84169766311291 25.8814484856022 31.10051893138574 21.176334919899155 24-25 21.66739022294578 26.089811423144756 31.155248044136936 21.08755030977253 26-27 21.459748980010833 26.294737299088588 31.136599409034876 21.1089143118657 28-29 21.265364265364266 26.542983765205985 31.143399810066473 21.04825215936327 30-31 21.450730339619227 26.164880387102606 31.038845927734815 21.345543345543348 32-33 21.334486242423857 26.342811970597204 30.91888723771243 21.40381454926651 34-35 21.792062762037975 26.345910251696647 30.669709257970407 21.192317728294967 36-37 21.903418559522585 26.14793512999191 30.671840806999473 21.27680550348603 38-39 21.65713456208319 26.54941733343162 30.65908319046859 21.134364914016604 40-41 21.759217594889368 26.490605015391864 30.430262211173588 21.31991517854518 42-43 21.667026974221578 26.652613164462156 30.088997473625938 21.591362387690328 44-45 21.871315726756322 26.780559654531338 29.76198605589491 21.58613856281744 46-47 22.072507602055673 27.190034206989367 29.240373292137818 21.497084898817135 48-49 22.319491462181908 27.288861149366024 28.756900017864854 21.634747370587213 50-51 22.105568765494294 27.59935567255069 28.592166229969905 21.702909331985108 52-53 22.42657017873512 27.64662335201498 28.17415040558306 21.75265606366684 54-55 22.05559181233071 27.616898702489866 28.30411911639601 22.023390368783414 56-57 22.15203412082393 27.813527580712925 28.18459289770582 21.849845400757324 58-59 22.410198664116095 27.464940961528896 28.155991805613965 21.96886856874104 60-61 22.28288889331088 27.35874911585349 28.63752797159154 21.72083401924409 62-63 22.076440258916243 27.55129867398812 28.55567771050964 21.81658335658599 64-65 22.040043529902384 27.526964205430023 28.45025586934516 21.982736395322437 66-67 22.14708555871619 27.696757008542754 28.060807785712022 22.09534964702903 68-69 22.187556810524857 27.509227612017916 28.253044139686285 22.05017143777094 70-71 22.22308647898929 27.462411990899376 28.127095791255456 22.187405738855873 72-73 22.224438113327004 27.470356803690137 28.14425903314792 22.16094604983494 74-75 22.028037805815583 27.911861800750685 27.885632885632887 22.17446750780084 76-77 22.087865225144068 27.764730994318658 27.990935538036236 22.15646824250104 78-79 22.127581629584068 27.83173643351251 27.821288932206574 22.21939300469685 80-81 22.04611726563811 28.038485179759277 27.69541672301553 22.219980831587083 82-83 22.16524989654073 27.85860792441481 27.735904098343973 22.240238080700493 84-85 22.056754428215754 27.93268524108986 27.592448119831488 22.418112210862894 86-87 22.017704654380235 27.789061973526692 27.521045364276 22.672188007817077 88-89 21.99519866898093 27.74051099298528 27.59308742231312 22.671202915720674 90-91 21.954570106846237 27.748494061057187 27.789430450431464 22.507505381665112 92-93 21.97677310817961 27.71801214708556 27.644207070273108 22.661007674461725 94-95 21.757519732117174 27.722742723583927 27.623562038355292 22.896175505943607 96-97 21.922229956079832 27.572242597092707 27.613580292140817 22.89194715468665 98-99 21.842313427469307 27.62511323769582 27.370936163111125 23.16163717172375 100-101 21.64437240166583 27.643290553913285 27.388206050387332 23.324130994033556 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 611.0 1 467.0 2 445.0 3 542.5 4 431.0 5 391.0 6 646.5 7 1218.5 8 1833.5 9 2082.5 10 1859.0 11 1604.5 12 1638.5 13 1730.0 14 1868.5 15 2049.5 16 2156.0 17 2304.0 18 2485.5 19 2669.5 20 2905.0 21 3157.5 22 3575.5 23 4324.5 24 5240.0 25 6306.5 26 7783.0 27 9651.0 28 11337.0 29 12958.5 30 14990.0 31 17160.5 32 19010.0 33 20424.0 34 22211.0 35 24465.5 36 27137.0 37 29134.5 38 29836.0 39 30522.5 40 31747.0 41 33682.0 42 35218.0 43 36493.0 44 38102.5 45 39066.5 46 38866.5 47 39536.5 48 42616.5 49 42512.5 50 39299.5 51 37469.5 52 36110.5 53 35237.0 54 34216.5 55 31409.5 56 28082.0 57 25528.0 58 23673.0 59 21581.0 60 18249.0 61 14763.5 62 12007.0 63 9313.5 64 6668.0 65 4944.5 66 3870.5 67 3170.5 68 2552.5 69 1906.0 70 1438.5 71 1153.0 72 974.5 73 851.5 74 691.0 75 531.5 76 391.5 77 257.5 78 174.0 79 124.5 80 100.5 81 82.5 82 51.5 83 26.0 84 16.5 85 11.0 86 9.5 87 6.5 88 3.0 89 2.5 90 1.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.107312440645774 2 0.009315787093564872 3 0.005788450232894677 4 0.010672455116899562 5 0.0 6 0.0 7 0.0 8 0.0 9 0.005878894767783657 10-11 2.71333604666938E-4 12-13 1.35666802333469E-4 14-15 0.0 16-17 0.0 18-19 4.5222267444489665E-5 20-21 0.0 22-23 0.0011305566861122417 24-25 0.0 26-27 9.044453488897933E-5 28-29 0.0 30-31 0.0 32-33 0.003708225930448153 34-35 0.001221001221001221 36-37 0.002125446569891014 38-39 4.5222267444489665E-5 40-41 0.0030298919187808078 42-43 0.010220232442454664 44-45 0.0019445575001130555 46-47 0.0025324469768914213 48-49 0.011531678198344865 50-51 9.044453488897933E-5 52-53 0.001763668430335097 54-55 0.009903676570343238 56-57 0.004477004477004476 58-59 0.00135666802333469 60-61 0.005788450232894677 62-63 0.0037534481978926428 64-65 0.018360240582462804 66-67 0.003165558721114277 68-69 9.044453488897933E-5 70-71 0.0010401121512232624 72-73 0.0 74-75 0.0 76-77 0.001221001221001221 78-79 0.011079455523899969 80-81 0.016777461221905664 82-83 0.012888346221679553 84-85 0.008818342151675486 86-87 0.010989010989010988 88-89 0.029982363315696647 90-91 0.025007913896802784 92-93 0.003165558721114277 94-95 0.008320897209786099 96-97 0.010989010989010988 98-99 0.010989010989010988 100-101 0.012119567675123231 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1105650.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.759832668221534 #Duplication Level Percentage of deduplicated Percentage of total 1 78.1570971245731 47.48812143123 2 12.950770590366531 15.7377330799039 3 4.061826606639189 7.403877148401815 4 1.6832905264841505 4.09105802884718 5 0.8694186307267296 2.6412865260795186 6 0.5233378802457832 1.907875321960533 7 0.35091069202547087 1.4924892450270204 8 0.23051248294842028 1.1204719913505827 9 0.1693241494708022 0.9259296289681387 >10 0.9203436106300187 10.025579510658329 >50 0.05705415725415526 2.340609247497854 >100 0.02462134738939835 2.765311634678803 >500 4.4766037384479985E-4 0.1609319689914376 >1k 7.461006230746665E-4 0.7940519348884565 >5k 2.984402492298666E-4 1.104673301516437 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6503 0.5881608103830326 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5681 0.5138154027042916 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3632 0.3284945507167729 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1488 0.13458146791480124 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1326 0.11992945326278659 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1218 0.11016144349477683 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.8088906977795866E-4 0.0 0.0 0.0 0.0 3 1.8088906977795866E-4 0.0 0.0 0.0 0.0 4 1.8088906977795866E-4 0.0 0.0 0.0 0.0 5 2.71333604666938E-4 0.0 0.0 0.0 0.0 6 3.617781395559173E-4 0.0 0.0 0.0 0.0 7 3.617781395559173E-4 0.0 0.0 0.0 0.0 8 4.5222267444489664E-4 0.0 0.0 0.0 0.0 9 4.5222267444489664E-4 0.0 0.0 0.0 0.0 10-11 4.974449418893864E-4 0.0 0.0 0.0 0.0 12-13 8.14000814000814E-4 0.0 0.0 0.0 0.0 14-15 9.044453488897933E-4 0.0 0.0 0.0 0.0 16-17 9.49667616334283E-4 0.0 0.0 0.0 0.0 18-19 0.0011757789535567313 0.0 0.0 0.0 0.0 20-21 0.00135666802333469 0.0 0.0 4.5222267444489665E-5 0.0 22-23 0.0015375570931126486 0.0 0.0 9.044453488897933E-5 0.0 24-25 0.0017184461628906072 0.0 0.0 3.1655587211142766E-4 0.0 26-27 0.0017184461628906072 0.0 0.0 3.617781395559173E-4 0.0 28-29 0.0018541129652240762 0.0 0.0 9.044453488897934E-4 0.0 30-31 0.001899335232668566 0.0 0.0 0.002442002442002442 0.0 32-33 0.0019445575001130558 0.0 0.0 0.0038438927327816216 0.0 34-35 0.0019897797675575454 0.0 0.0 0.00547189436078325 0.0 36-37 0.0022611133722244834 0.0 0.0 0.0073712295934518154 0.0 38-39 0.0023515579071134627 0.0 0.0 0.009768009768009768 0.0 40-41 0.0023515579071134627 0.0 0.0 0.013069235291457513 0.0 42-43 0.00275855831411387 0.0 0.0 0.019400352733686066 0.0 44-45 0.003346447790892235 0.0 0.0 0.029620585176140732 0.0 46-47 0.0038438927327816216 0.0 0.0 0.04006692895581784 0.0 48-49 0.0038891150002261115 0.0 0.0 0.06145706145706146 0.0 50-51 0.0038891150002261115 0.0 0.0 0.07986252430696875 0.0 52-53 0.0038891150002261115 0.0 0.0 0.10211187988965767 0.0 54-55 0.00402478180255958 0.0 0.0 0.1456609234387012 0.0 56-57 0.00407000407000407 0.0 0.0 0.1864061864061864 0.0 58-59 0.00407000407000407 0.0 0.0 0.24243657576990912 0.0 60-61 0.00407000407000407 0.0 0.0 0.3019490797268575 0.0 62-63 0.00407000407000407 0.0 0.0 0.38524849635960745 0.0 64-65 0.00407000407000407 0.0 0.0 0.4517704517704518 0.0 66-67 0.004250893139782028 0.0 0.0 0.5479129923574368 0.0 68-69 0.004250893139782028 0.0 0.0 0.6746257857368969 0.0 70-71 0.0043865599421154975 0.0 0.0 0.7598245376023154 0.0 72-73 0.004477004477004477 0.0 0.0 0.8786686564464343 0.0 74-75 0.004612671279337946 0.0 0.0 1.017546239768462 0.0 76-77 0.004612671279337946 0.0 0.0 1.1375661375661377 0.0 78-79 0.004612671279337946 0.0 0.0 1.249084249084249 0.0 80-81 0.004884004884004884 0.0 0.0 1.4060507393840727 0.0 82-83 0.005064893953782843 0.0 0.0 1.6237055125944013 0.0 84-85 0.005200560756116312 0.0 0.0 1.874689096911319 0.0 86-87 0.00551711662822774 0.0 0.0 2.1711662822773934 0.0 88-89 0.005698005698005698 0.0 0.0 2.5103785103785103 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4605 0.0 38.072014 1 GTATCAA 7305 0.0 36.098965 1 TCAACGC 9420 0.0 27.981167 4 ATCAACG 9430 0.0 27.853287 3 TATCAAC 9490 0.0 27.680943 2 CAACGCA 9550 0.0 27.545557 5 AACGCAG 9775 0.0 26.911514 6 GTACATG 5835 0.0 25.025076 1 TACATGG 5905 0.0 24.294558 2 ACGCAGA 10895 0.0 23.883528 7 CGCAGAG 11010 0.0 23.590935 8 ACATGGG 5815 0.0 23.11531 3 GAGTACT 6465 0.0 23.025772 12-13 GCAGAGT 11745 0.0 21.872046 9 GTGTAGG 540 0.0 21.348143 1 AGAGTAC 9865 0.0 21.27504 10-11 AGTACTT 6810 0.0 21.092276 12-13 GTACTTT 7025 0.0 21.088875 14-15 TAAGGCG 275 1.2641976E-9 20.720188 5 CATGGGA 2830 0.0 19.63465 4 >>END_MODULE