FastQCFastQC Report
Thu 26 May 2016
SRR936749_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936749_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1178380
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60980.5174901135457153No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48620.4126003496325464No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29570.25093772806734665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46100.035.9765241
GTATCAA70950.033.556761
ATCAACG88500.026.7948883
CAACGCA89400.026.525145
TCAACGC89500.026.4446544
TATCAAC90100.026.2135752
AACGCAG91700.026.0153126
GTACATG58300.025.5135251
TACATGG59300.024.8428652
ACATGGG58800.024.2459163
GAGTACT55850.023.4844612-13
ACGCAGA102700.023.229857
CGCAGAG103450.022.8767078
GTGTAGC6100.021.8133751
GCAGAGT107550.021.3860039
GTACTTT61900.021.3810614-15
AGAGTAC86050.021.26021610-11
AGTACTT60050.020.69442412-13
CATGGGG26800.020.3936334
CATGGGA27700.019.559454
Produced by FastQC (version 0.11.5)