##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936749_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1178380 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.36208353841715 31.0 31.0 34.0 30.0 34.0 2 31.408532052478826 31.0 31.0 34.0 28.0 34.0 3 31.43430642067924 31.0 31.0 34.0 28.0 34.0 4 35.08981483053005 37.0 35.0 37.0 32.0 37.0 5 35.068923437261326 37.0 35.0 37.0 32.0 37.0 6 35.174933383119196 37.0 35.0 37.0 32.0 37.0 7 35.05059743037051 37.0 35.0 37.0 32.0 37.0 8 35.01274376686638 37.0 35.0 37.0 32.0 37.0 9 36.50806531000187 39.0 37.0 39.0 32.0 39.0 10-11 36.391656341757326 39.0 37.0 39.0 32.0 39.0 12-13 36.281054498548855 39.0 35.0 39.0 32.0 39.0 14-15 37.30619452129194 39.5 37.0 41.0 32.0 41.0 16-17 37.19381311631223 39.0 36.0 41.0 31.0 41.0 18-19 37.17174765355827 39.0 36.0 41.0 31.0 41.0 20-21 37.11161382576079 39.0 36.0 41.0 31.0 41.0 22-23 36.960006110083334 39.0 36.0 41.0 31.0 41.0 24-25 37.07036652013782 39.0 36.0 41.0 31.0 41.0 26-27 37.026319183964425 39.0 36.0 41.0 31.0 41.0 28-29 36.91497776608564 39.0 36.0 41.0 30.5 41.0 30-31 36.697252159744735 39.0 36.0 41.0 30.0 41.0 32-33 36.47635567473989 39.0 35.0 40.5 30.0 41.0 34-35 36.23089538179535 39.0 35.0 40.0 30.0 41.0 36-37 36.03495052529744 38.0 35.0 40.0 29.5 41.0 38-39 35.815872638707376 38.0 35.0 40.0 28.5 41.0 40-41 35.60235577657462 38.0 35.0 40.0 27.0 41.0 42-43 35.33652005295406 38.0 34.5 40.0 27.0 41.0 44-45 35.022936149629146 38.0 34.0 40.0 25.5 41.0 46-47 34.67833975457832 38.0 34.0 40.0 24.0 41.0 48-49 34.53376499940596 38.0 34.0 40.0 24.0 41.0 50-51 33.59656562399226 37.5 32.5 39.5 22.5 40.5 52-53 33.74685755019603 37.5 33.0 39.5 23.0 40.5 54-55 34.16376550857957 38.0 33.5 40.0 23.0 41.0 56-57 34.07167382338465 38.0 33.5 40.0 22.5 41.0 58-59 33.81859077716865 38.0 33.0 40.0 21.5 41.0 60-61 33.49904445085626 37.0 33.0 40.0 20.5 41.0 62-63 33.18148687180705 37.0 32.5 40.0 19.0 41.0 64-65 32.84074534530457 36.0 32.0 39.5 18.0 41.0 66-67 32.471447665438994 36.0 31.5 39.0 16.5 41.0 68-69 32.066572752422815 35.0 31.0 39.0 13.0 40.5 70-71 31.631792375973795 35.0 31.0 38.5 9.5 40.0 72-73 31.24901474906227 35.0 31.0 37.5 7.5 40.0 74-75 30.814398156791526 35.0 30.0 37.0 7.0 39.0 76-77 30.37258863863949 34.5 30.0 36.5 3.5 39.0 78-79 29.925896994178448 34.0 29.0 36.0 2.0 39.0 80-81 29.47059055652676 34.0 29.0 35.5 2.0 37.5 82-83 28.979654695429318 34.0 29.0 35.0 2.0 37.0 84-85 28.634854206622652 34.0 28.5 35.0 2.0 36.5 86-87 28.291006296780324 34.0 27.5 35.0 2.0 36.0 88-89 27.884903851049746 34.0 27.0 35.0 2.0 36.0 90-91 27.462764982433512 33.0 26.0 35.0 2.0 35.0 92-93 27.087624959690423 33.0 25.5 35.0 2.0 35.0 94-95 26.624896468032382 33.0 25.0 35.0 2.0 35.0 96-97 26.023851389195336 33.0 24.0 35.0 2.0 35.0 98-99 25.302788574144163 32.5 19.0 35.0 2.0 35.0 100-101 22.88887328366062 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4160332854071065 1101 2 0.5015652883469635 1101 3 0.5030620781137749 1101 4 0.5300297543431185 1101 5 0.513657403990976 1101 6 0.39188320549063604 1101 7 0.4521493688868503 1101 8 0.5952601193759008 1101 9 0.6350384056059397 1101 10-11 0.6915896025075412 1101 12-13 0.8670891274761701 1101 14-15 0.9980243862391305 1101 16-17 0.9924952323877037 1101 18-19 0.9468588520621708 1101 20-21 0.8398768776729924 1101 22-23 0.8381356563867755 1101 24-25 0.9552101756580385 1101 26-27 0.7328472677441198 1101 28-29 0.640476430666638 1101 30-31 0.6400974748734782 1101 32-33 0.5749640900324451 1101 34-35 0.4936156315460707 1101 36-37 0.5544878251411873 1101 38-39 0.4192709688648222 1101 40-41 0.48582871098400915 1101 42-43 0.4630411192407138 1101 44-45 0.5668183327936234 1101 46-47 0.7941220252925589 1101 48-49 0.759352089584489 1101 50-51 0.7846315788345777 1101 52-53 0.8642821316124056 1101 54-55 1.0052829817607432 1101 56-57 0.7511835935119748 1101 58-59 0.8454233582353865 1101 60-61 0.7755650326779673 1101 62-63 0.7506144347838202 1101 64-65 0.8254455177245603 1101 66-67 0.8091438599763663 1101 68-69 0.724675986426508 1101 70-71 0.7580557688115768 1101 72-73 0.7332677309113826 1101 74-75 0.6598865071166813 1101 76-77 0.643996277044625 1101 78-79 0.5463142278710009 1101 80-81 0.7845211440086608 1101 82-83 0.8428211235428869 1101 84-85 0.7079562185850747 1101 86-87 0.8341453383468753 1101 88-89 0.9323290454033035 1101 90-91 0.9077411457130751 1101 92-93 0.9592621956595089 1101 94-95 0.9926664653218289 1101 96-97 0.6806416093799044 1101 98-99 0.7491240113307462 1101 100-101 1.1710997921923685 1102 1 0.17525985497384866 1102 2 0.16359271076855464 1102 3 0.10351942614131815 1102 4 0.09033185325213111 1102 5 0.21644810381191348 1102 6 0.1117298485284195 1102 7 0.23743577674345318 1102 8 -0.006799792754208056 1102 9 0.24565801757544392 1102 10-11 0.19932782960322015 1102 12-13 0.1463108282016421 1102 14-15 0.16743410269258874 1102 16-17 0.10343789543620119 1102 18-19 0.12010653070941402 1102 20-21 0.30523887948208284 1102 22-23 0.09349772742109508 1102 24-25 -0.22481869126337273 1102 26-27 -0.0980406842966417 1102 28-29 -0.046152485173941216 1102 30-31 -0.19490303277821397 1102 32-33 -0.0657252317448993 1102 34-35 -0.4116865596632451 1102 36-37 -0.20020795304270678 1102 38-39 -0.3521536633184468 1102 40-41 -0.2478491332499928 1102 42-43 -0.19378056579371616 1102 44-45 -0.5492095003643342 1102 46-47 -0.5914019404824913 1102 48-49 -0.246241885974392 1102 50-51 -0.2613432047212001 1102 52-53 -0.39186103078075263 1102 54-55 -0.208935073102559 1102 56-57 -0.1745654234897387 1102 58-59 -0.012126114729859694 1102 60-61 -0.09559585137438376 1102 62-63 -0.16555207958733575 1102 64-65 -0.13267725553049559 1102 66-67 -0.11887112959369972 1102 68-69 -0.3474497819735589 1102 70-71 -0.3763571320025143 1102 72-73 -0.5194199386904366 1102 74-75 -0.4353806792007191 1102 76-77 -0.3043859293019473 1102 78-79 -0.3427269565265547 1102 80-81 -0.05151485772658404 1102 82-83 -0.228295110235333 1102 84-85 0.058805842922819096 1102 86-87 -0.06267214702352675 1102 88-89 0.010461833241816265 1102 90-91 -0.02489866617889902 1102 92-93 -0.22966269034960973 1102 94-95 -0.23305451546125155 1102 96-97 -0.26830939761645567 1102 98-99 -0.28322840047631104 1102 100-101 -0.18021306431757367 1103 1 0.11486395270107863 1103 2 0.34128927485994254 1103 3 0.31997770580922236 1103 4 0.37289025775407936 1103 5 0.37938997671896146 1103 6 0.3417675464300203 1103 7 0.26295368943887354 1103 8 0.21366853308248324 1103 9 0.4514100436518902 1103 10-11 0.49469720464036726 1103 12-13 0.32543716323245775 1103 14-15 0.29629244081453265 1103 16-17 0.6098009643723401 1103 18-19 0.2943187634555642 1103 20-21 0.6288922073993319 1103 22-23 0.46689457585669913 1103 24-25 0.5871791241407465 1103 26-27 0.34640878268178454 1103 28-29 0.2258117439262648 1103 30-31 0.2661903549801181 1103 32-33 0.33748565358612126 1103 34-35 0.20378431734549451 1103 36-37 0.36288339873171793 1103 38-39 0.4348432851825521 1103 40-41 0.3702113072358699 1103 42-43 0.2678713552636083 1103 44-45 0.29367916945950867 1103 46-47 0.41623299918105516 1103 48-49 0.5096363364283647 1103 50-51 0.5776190131637549 1103 52-53 0.3879554829807148 1103 54-55 0.6321844951301472 1103 56-57 0.5612650449351761 1103 58-59 0.3149403346330928 1103 60-61 0.19013170326527984 1103 62-63 0.439525867387907 1103 64-65 0.3800111101341912 1103 66-67 0.15697868315522356 1103 68-69 0.28505883752037064 1103 70-71 0.41644497135215275 1103 72-73 0.6081207066817882 1103 74-75 0.49619218029162226 1103 76-77 0.34131014436995954 1103 78-79 0.4652205636351354 1103 80-81 0.27972325331627346 1103 82-83 0.2357531787455862 1103 84-85 0.3658406379373851 1103 86-87 0.3300752175909736 1103 88-89 0.404629680547977 1103 90-91 0.5726533232399262 1103 92-93 0.618156181388354 1103 94-95 0.5901288271190488 1103 96-97 0.6925530223807712 1103 98-99 0.7848656015448121 1103 100-101 1.0792758096000377 1104 1 0.3032700660000103 1104 2 0.3167914893490291 1104 3 0.3285356697883195 1104 4 0.49135860583249524 1104 5 0.43838961127372045 1104 6 0.30744910180860785 1104 7 0.452642270093186 1104 8 0.43078800640497406 1104 9 0.583457818799431 1104 10-11 0.5758918424132276 1104 12-13 0.25628449166409695 1104 14-15 0.521564547524541 1104 16-17 0.3417373332647671 1104 18-19 0.5656874240714771 1104 20-21 0.7439072342895585 1104 22-23 0.5638135861000819 1104 24-25 0.6151817068022893 1104 26-27 0.27675771470897104 1104 28-29 0.5151499471356331 1104 30-31 0.5764833657034885 1104 32-33 0.21881787876584013 1104 34-35 0.1929992509653644 1104 36-37 0.3075392393103229 1104 38-39 0.3938875095113872 1104 40-41 0.37282158170161495 1104 42-43 0.4678742188270846 1104 44-45 0.5135224370854203 1104 46-47 0.5548778791295632 1104 48-49 0.4630260455994062 1104 50-51 0.44537453847765107 1104 52-53 0.6076930714784581 1104 54-55 0.6792113662643331 1104 56-57 0.7126115333881913 1104 58-59 0.7917816059461842 1104 60-61 0.7417037996119191 1104 62-63 0.8262484099050127 1104 64-65 0.6338806707271942 1104 66-67 0.8678562699075627 1104 68-69 0.8143128655996499 1104 70-71 0.7309388978703915 1104 72-73 0.6535787541133082 1104 74-75 0.501960897087919 1104 76-77 0.7338675383635653 1104 78-79 0.49620091140810274 1104 80-81 0.7204798749659069 1104 82-83 0.6406992346641651 1104 84-85 0.5911894472404207 1104 86-87 0.7081900246686104 1104 88-89 0.6318940092741521 1104 90-91 0.6775896294066825 1104 92-93 0.7427375556806446 1104 94-95 0.5814982513518849 1104 96-97 0.5564775088483884 1104 98-99 0.7259621009066493 1104 100-101 0.9023730398523568 1105 1 0.2902243720981943 1105 2 0.2634103876920655 1105 3 0.35525710530880517 1105 4 0.46878266809603275 1105 5 0.5332905486241231 1105 6 0.34057756668499195 1105 7 0.2216409883784678 1105 8 0.4128910995068793 1105 9 0.5059480515155883 1105 10-11 0.4724323708503064 1105 12-13 0.4536921078291485 1105 14-15 0.15159373355847805 1105 16-17 0.35719164708412166 1105 18-19 0.2563965365998513 1105 20-21 0.22170615950227557 1105 22-23 0.22407541107652662 1105 24-25 0.40867546037333113 1105 26-27 0.4022924059392565 1105 28-29 0.1703659855561952 1105 30-31 0.19750811978412486 1105 32-33 0.07422385804220966 1105 34-35 0.23498814791859246 1105 36-37 0.48022597587934257 1105 38-39 0.4866334674773256 1105 40-41 0.23998150263679463 1105 42-43 0.4886252573248555 1105 44-45 0.20937230659760075 1105 46-47 0.4790308664513958 1105 48-49 0.45819838218078246 1105 50-51 0.6172212114242086 1105 52-53 0.5396324757127573 1105 54-55 0.6279138231415899 1105 56-57 0.6589841263125109 1105 58-59 0.85580325611528 1105 60-61 0.44084211670504914 1105 62-63 0.4347115876309644 1105 64-65 0.5505363990654502 1105 66-67 0.5667085175410236 1105 68-69 0.5132624000129162 1105 70-71 0.5504696049754081 1105 72-73 0.5487043775980318 1105 74-75 0.7108487393289167 1105 76-77 0.44957819512654495 1105 78-79 0.4770473836041589 1105 80-81 0.5212600994976171 1105 82-83 0.6752622934840602 1105 84-85 0.7884368490657074 1105 86-87 0.7852886394901759 1105 88-89 0.5865186058428655 1105 90-91 0.5688890256109538 1105 92-93 0.5618235232208342 1105 94-95 0.6856630520684099 1105 96-97 1.0281801819184793 1105 98-99 0.7553469550536889 1105 100-101 1.1442287465713186 1106 1 0.1720787878733141 1106 2 0.2742576901693212 1106 3 0.3057043086194433 1106 4 0.051929110493574626 1106 5 0.1836233289128515 1106 6 0.1932617400740142 1106 7 0.4054796900585771 1106 8 0.218695207293365 1106 9 0.4662585193150406 1106 10-11 0.3114100965020441 1106 12-13 0.39463981650813906 1106 14-15 0.5689078438241353 1106 16-17 0.6928463374744922 1106 18-19 0.6146597858322664 1106 20-21 0.38480044407233294 1106 22-23 0.34436118257374915 1106 24-25 0.1809493318762776 1106 26-27 0.02982639911201801 1106 28-29 0.3724975911018973 1106 30-31 0.3451386097053657 1106 32-33 0.3301447726635658 1106 34-35 0.17749868437186223 1106 36-37 0.3331127733696633 1106 38-39 0.29184829670096235 1106 40-41 0.010116617155176755 1106 42-43 -0.1524588054552467 1106 44-45 0.20501183593756878 1106 46-47 0.3523049858708944 1106 48-49 0.3069430662778885 1106 50-51 0.1701270794181795 1106 52-53 0.2878379413191041 1106 54-55 0.40494387825534517 1106 56-57 0.2778553432013453 1106 58-59 0.4760170364612222 1106 60-61 0.5136752307275927 1106 62-63 0.6444251486661159 1106 64-65 0.5590680656367581 1106 66-67 0.6632658148692414 1106 68-69 0.533808483884993 1106 70-71 0.4705861257527246 1106 72-73 0.6464998273758589 1106 74-75 0.5287645239847905 1106 76-77 0.48188315155044137 1106 78-79 0.595977242291724 1106 80-81 0.6555665052797934 1106 82-83 0.7478980277938057 1106 84-85 0.5882604469069257 1106 86-87 0.7597308272583518 1106 88-89 0.7813738607201479 1106 90-91 0.6609733363075989 1106 92-93 0.635901941131852 1106 94-95 0.8038639407538817 1106 96-97 1.139775048138894 1106 98-99 1.258944128805048 1106 100-101 1.5274867514152284 1107 1 0.22875820668465963 1107 2 0.21273825280940173 1107 3 0.006267206318906204 1107 4 0.26169867022256454 1107 5 0.2774900625604815 1107 6 0.2644934169166362 1107 7 0.24617770896782076 1107 8 0.39454599431967097 1107 9 0.4745570740719742 1107 10-11 0.4780268494710995 1107 12-13 0.3223438508229961 1107 14-15 0.2520589116552401 1107 16-17 0.26189469257663944 1107 18-19 0.42678462804057204 1107 20-21 0.5538613581048466 1107 22-23 0.5071780540475928 1107 24-25 0.4182809133998262 1107 26-27 0.2656154863676008 1107 28-29 0.3708520492001526 1107 30-31 0.4191123196778008 1107 32-33 0.18473973105808028 1107 34-35 0.33051959016055577 1107 36-37 0.3478695165491956 1107 38-39 0.4294345990942503 1107 40-41 0.3988122917307493 1107 42-43 0.245036170972611 1107 44-45 0.39294012766541897 1107 46-47 0.33940148997991315 1107 48-49 0.33122849534247223 1107 50-51 0.35264215013462064 1107 52-53 0.5218816592777245 1107 54-55 0.6003543942663683 1107 56-57 0.4583642052978547 1107 58-59 0.3490220986762296 1107 60-61 0.5227675742884017 1107 62-63 0.5268880515179575 1107 64-65 0.5449827421959981 1107 66-67 0.4548085023515327 1107 68-69 0.4636829589861087 1107 70-71 0.6510354134359524 1107 72-73 0.49047926937292274 1107 74-75 0.4504145098157366 1107 76-77 0.30731723970768954 1107 78-79 0.399890476184094 1107 80-81 0.5994812204818949 1107 82-83 0.871020628716078 1107 84-85 0.8495441628046017 1107 86-87 0.681198869610931 1107 88-89 0.5706978038378523 1107 90-91 0.8239157970850925 1107 92-93 0.47095810209225775 1107 94-95 0.49245560162411905 1107 96-97 0.6401276642606994 1107 98-99 0.7542409703950739 1107 100-101 1.4055942816210667 1108 1 0.04287565095034651 1108 2 0.20983470573210283 1108 3 0.06315706561051115 1108 4 0.25828246960456624 1108 5 0.2372036660743575 1108 6 0.23714082875044085 1108 7 -0.028035754093032494 1108 8 0.031597502274422595 1108 9 0.04450076517746737 1108 10-11 -0.11656411301517267 1108 12-13 0.13605046999100523 1108 14-15 0.17487678238737914 1108 16-17 0.07291642449011704 1108 18-19 0.22563035954546962 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-2.989375137897376 2316 56-57 -2.1966958042220703 2316 58-59 -2.303198933555514 2316 60-61 -1.849559197097424 2316 62-63 -1.879081450726023 2316 64-65 -2.4932287021124466 2316 66-67 -2.3499820593239704 2316 68-69 -3.1549080922854777 2316 70-71 -3.047344499524737 2316 72-73 -2.452592436053436 2316 74-75 -2.124040644543026 2316 76-77 -1.9334858338677172 2316 78-79 -2.511756980636253 2316 80-81 -2.0797950953075777 2316 82-83 -2.2130946983613065 2316 84-85 -1.8349341113692077 2316 86-87 -2.451514818679563 2316 88-89 -2.9126508293730815 2316 90-91 -2.638969249834531 2316 92-93 -3.2590840575704654 2316 94-95 -3.654843969979307 2316 96-97 -4.446495142756845 2316 98-99 -5.4611473064987095 2316 100-101 -5.500672718242935 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2616.0 3 1085.0 4 1085.0 5 1079.0 6 1299.0 7 1554.0 8 2005.0 9 2357.0 10 3312.0 11 4136.0 12 5456.0 13 6785.0 14 7616.0 15 9348.0 16 11505.0 17 14364.0 18 12016.0 19 8194.0 20 8182.0 21 8781.0 22 9691.0 23 10849.0 24 12462.0 25 14256.0 26 16777.0 27 19571.0 28 22981.0 29 27912.0 30 33630.0 31 40536.0 32 51036.0 33 64637.0 34 84221.0 35 114175.0 36 155917.0 37 201421.0 38 159960.0 39 25563.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.20342758571511 18.71453650850784 14.750944844189846 22.331091061587202 2 15.325543336174086 20.07058871463349 40.92405346950832 23.6798144796841 3 19.08704852590541 25.070345544103155 30.001536355171364 25.841069574820075 4 12.393218635083468 16.69546434451249 37.96139611142196 32.94992090898207 5 13.500595646979608 37.48024770083662 34.10250901961517 14.916647632568603 6 31.06068646388317 36.325783787225916 17.831419413725033 14.782110335165886 7 27.753823894522156 33.49055722638373 20.926344497786413 17.829274381307705 8 26.195139718449052 34.251544998314706 20.13180136742645 19.421513915809797 9 26.663666850617652 16.574181771872478 20.750859617098953 36.01129176041092 10-11 24.778907210866333 26.667456321676582 27.32350679846264 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21.92475313068903 26.28060139782984 30.046806506231672 21.747838965249457 38-39 21.658038695802176 26.595736103337046 30.028563753695657 21.717661447165117 40-41 21.908989342359465 26.512731927901278 29.846612633782733 21.731666095956527 42-43 21.75031219356128 26.662412149813875 29.525003277873274 22.062272378751572 44-45 21.887220457045093 26.810791681942238 29.33164283677543 21.97034502423724 46-47 22.02713734835061 27.027790790036455 28.896294561328475 22.04877730028446 48-49 22.207389988411904 27.121616443808342 28.560941881735236 22.110051686044514 50-51 21.970113890035712 27.4522042459373 28.457148773287926 22.120533090739055 52-53 22.279501872137903 27.514571445059627 28.02071063163862 22.18521605116385 54-55 21.978051355516783 27.34894288022704 28.224868793579883 22.4481369706763 56-57 22.076484690203184 27.628675838695756 28.03094764100838 22.263891830092682 58-59 22.264724067355854 27.257130174890804 28.108053514981336 22.37009224277201 60-61 22.079929793977744 27.24705738569985 28.54114143347763 22.13187138684477 62-63 22.010223951815796 27.41350653031902 28.403779475970488 22.172490041894694 64-65 21.948149980545317 27.357909739604263 28.30611869945124 22.387821580399184 66-67 21.929810044534403 27.6094636139701 28.043562667481396 22.417163674014102 68-69 22.00009081792576 27.373583781446236 28.251249913532 22.375075487096 70-71 22.018224437074178 27.278733573904272 28.14960095323124 22.553441035790303 72-73 22.012665081409892 27.398082755834814 28.140938492376684 22.44831367037861 74-75 21.83558295745304 27.686246387942408 27.964772626013605 22.51339802859095 76-77 21.95640993434587 27.496910793213253 28.008921357621514 22.537757914819366 78-79 21.967475645348408 27.59079263050605 27.86397887834938 22.577752845796166 80-81 21.896410149776894 27.7465993070246 27.772272921126838 22.584717622071672 82-83 22.034139963922495 27.63459791317381 27.8193137296088 22.511948393294897 84-85 22.03908224705521 27.570103770045712 27.683626129070042 22.707187853829037 86-87 21.93845964530385 27.49941124427952 27.78468725932621 22.77744185109042 88-89 21.934428232929818 27.465857939153636 27.7166828480063 22.88303097991024 90-91 21.857606526505442 27.429840533261597 27.952018677225084 22.760534263007884 92-93 21.98520250014429 27.42299087060293 27.85552533925437 22.736281289998402 94-95 21.799547299711524 27.389555642500866 27.86330386339892 22.947593194388688 96-97 21.81489578753864 27.291375631648542 27.961894852265957 22.931833728546867 98-99 21.833741619938646 27.36483676175246 27.63722589199164 23.164195726317256 100-101 21.865144081588646 27.26679225644765 27.703795454487572 23.16426820747613 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 391.0 1 295.5 2 333.5 3 441.0 4 389.5 5 404.0 6 613.0 7 1124.0 8 1767.0 9 2065.5 10 1802.0 11 1525.0 12 1583.5 13 1701.0 14 1787.0 15 1912.5 16 1988.0 17 2040.0 18 2179.0 19 2338.0 20 2655.5 21 3070.5 22 3465.0 23 4134.5 24 5052.0 25 6231.0 26 7876.0 27 9822.0 28 11512.5 29 13130.0 30 15265.0 31 17589.0 32 19694.0 33 21612.5 34 23579.5 35 25635.5 36 28347.5 37 30754.0 38 32006.0 39 32960.5 40 34123.0 41 35944.5 42 37491.0 43 38919.5 44 40569.5 45 41697.0 46 41901.5 47 42624.5 48 45853.0 49 45760.0 50 42318.0 51 40425.0 52 39039.5 53 38568.5 54 37634.5 55 34277.5 56 30806.0 57 27938.0 58 25724.0 59 23314.5 60 19644.0 61 16059.0 62 13036.5 63 10191.5 64 7498.5 65 5720.5 66 4479.5 67 3539.0 68 2726.5 69 2056.5 70 1611.5 71 1289.0 72 1047.5 73 921.0 74 751.5 75 564.0 76 427.0 77 292.0 78 196.5 79 140.0 80 104.5 81 80.0 82 55.0 83 40.5 84 31.0 85 19.5 86 13.5 87 11.0 88 6.5 89 4.0 90 3.0 91 1.5 92 0.5 93 2.0 94 3.5 95 3.0 96 2.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03462380556357032 2 0.024440333338990816 3 0.022658225699689404 4 0.05286919329927528 5 0.05677285765203075 6 0.05134167246558835 7 0.055499923623958315 8 0.04641966089037492 9 0.04497700232522616 10-11 0.05520290568407475 12-13 0.044213241908382696 14-15 0.04030957755562722 16-17 0.04697126563587298 18-19 0.05359052258184965 20-21 0.011795855326804596 22-23 0.020579100120504422 24-25 0.00598278993194046 26-27 0.01196557986388092 28-29 0.007340586228551062 30-31 0.009207556136390639 32-33 0.004030957755562722 34-35 0.010013747687503183 36-37 0.01060778356727032 38-39 0.011753424192535514 40-41 0.00199426331064682 42-43 0.0020791255791849828 44-45 0.002376143519068552 46-47 3.3944907415265024E-4 48-49 0.0013153651623415198 50-51 5.516047454980567E-4 52-53 0.004073388889831803 54-55 0.007128430557205656 56-57 0.016675435767748943 58-59 0.011074526044230214 60-61 0.011329112849844702 62-63 0.005813065394864135 64-65 4.243113426908128E-5 66-67 0.006576825811707598 68-69 0.016845160304825267 70-71 0.006110083334747704 72-73 0.0018669699078395765 74-75 0.0029701793988356896 76-77 0.007128430557205656 78-79 0.005346322917904242 80-81 0.010819939238615727 82-83 0.007085999422936574 84-85 0.016802729170556186 86-87 0.004030957755562722 88-89 0.005770634260595054 90-91 0.003946095487024559 92-93 0.017651351855937813 94-95 0.009928885418965019 96-97 0.009674298613350531 98-99 0.024270608801914492 100-101 0.03997012848147456 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1178380.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.831704722949745 #Duplication Level Percentage of deduplicated Percentage of total 1 78.85502105192619 48.75740377604686 2 12.493838148028438 15.450306224504393 3 3.860181803141174 7.160448642861864 4 1.724248258229923 4.26452836687732 5 0.8740230564449183 2.7021167773576114 6 0.5118425176487527 1.8988857249545323 7 0.3343078818245559 1.4469578364871498 8 0.2237832151784109 1.1069518146291064 9 0.16492428100506107 0.9177794500274752 >10 0.881483448707562 9.879867589569281 >50 0.05115261033770176 2.1536178445482936 >100 0.023817024595163418 2.5787474061143048 >500 5.506811728707265E-4 0.21732128911119958 >1k 6.883514660884081E-4 0.9459817806082103 >5k 1.3767029321768164E-4 0.5190854763024265 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6098 0.5174901135457153 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4862 0.4126003496325464 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2957 0.25093772806734665 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 8.486226853816256E-5 0.0 0.0 0.0 0.0 3 8.486226853816256E-5 0.0 0.0 0.0 0.0 4 8.486226853816256E-5 0.0 0.0 0.0 0.0 5 8.486226853816256E-5 0.0 0.0 0.0 0.0 6 1.6972453707632512E-4 0.0 0.0 0.0 0.0 7 2.5458680561448767E-4 0.0 0.0 0.0 0.0 8 2.5458680561448767E-4 0.0 0.0 0.0 0.0 9 2.5458680561448767E-4 0.0 0.0 0.0 0.0 10-11 2.5458680561448767E-4 0.0 0.0 0.0 0.0 12-13 5.091736112289753E-4 0.0 0.0 0.0 0.0 14-15 8.910538196507068E-4 0.0 0.0 0.0 0.0 16-17 9.759160881888695E-4 0.0 0.0 0.0 0.0 18-19 0.0011456406252651945 0.0 0.0 1.2729340280724383E-4 0.0 20-21 0.001188071759534276 0.0 0.0 1.6972453707632512E-4 0.0 22-23 0.001188071759534276 0.0 0.0 2.5458680561448767E-4 0.0 24-25 0.001357796296610601 0.0 0.0 5.091736112289753E-4 0.0 26-27 0.001357796296610601 0.0 0.0 6.364670140362192E-4 0.0 28-29 0.0014850896994178448 0.0 0.0 0.001188071759534276 0.0 30-31 0.001527520833686926 0.0 0.0 0.0026307303246830395 0.0 32-33 0.001527520833686926 0.0 0.0 0.0041582511583699655 0.0 34-35 0.0015699519679560074 0.0 0.0 0.005897927663402298 0.0 36-37 0.001782107639301414 0.0 0.0 0.008443795719547174 0.0 38-39 0.002121556713454064 0.0 0.0 0.01128668171557562 0.0 40-41 0.002163987847723145 0.0 0.0 0.014638741322833042 0.0 42-43 0.0023337123847994704 0.0 0.0 0.020112357643544528 0.0 44-45 0.002715592593221202 0.0 0.0 0.029022895840051598 0.0 46-47 0.0033944907415265025 0.0 0.0 0.04030957755562722 0.0 48-49 0.003564215278602828 0.0 0.0 0.062161611704204076 0.0 50-51 0.003564215278602828 0.0 0.0 0.0836317656443592 0.0 52-53 0.003564215278602828 0.0 0.0 0.10425329689913271 0.0 54-55 0.00364907754714099 0.0 0.0 0.14609039528844686 0.0 56-57 0.00364907754714099 0.0 0.0 0.18890340976594988 0.0 58-59 0.00364907754714099 0.0 0.0 0.242748519153414 0.0 60-61 0.00364907754714099 0.0 0.0 0.3052495799317707 4.243113426908128E-5 62-63 0.00364907754714099 0.0 0.0 0.38255910657003683 8.486226853816256E-5 64-65 0.00364907754714099 0.0 0.0 0.4516369931601012 8.486226853816256E-5 66-67 0.0038188020842173155 0.0 0.0 0.5437974167925457 8.486226853816256E-5 68-69 0.0038188020842173155 0.0 0.0 0.6649383051307727 8.486226853816256E-5 70-71 0.003988526621293641 0.0 0.0 0.7524737351278874 8.486226853816256E-5 72-73 0.004073388889831803 0.0 0.0 0.8708141686043551 8.486226853816256E-5 74-75 0.004073388889831803 0.0 0.0 1.011176360766476 8.486226853816256E-5 76-77 0.004073388889831803 0.0 0.0 1.127989273409257 8.486226853816256E-5 78-79 0.0041582511583699655 0.0 0.0 1.2366129771381047 8.486226853816256E-5 80-81 0.004497700232522616 0.0 0.0 1.392759551248324 8.486226853816256E-5 82-83 0.004497700232522616 0.0 0.0 1.615141125952579 8.486226853816256E-5 84-85 0.004582562501060778 0.0 0.0 1.8570410224206113 8.486226853816256E-5 86-87 0.004709855903868023 0.0 0.0 2.1566472614945944 8.486226853816256E-5 88-89 0.004752287038137104 0.0 0.0 2.47415943923013 8.486226853816256E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4610 0.0 35.976524 1 GTATCAA 7095 0.0 33.55676 1 ATCAACG 8850 0.0 26.794888 3 CAACGCA 8940 0.0 26.52514 5 TCAACGC 8950 0.0 26.444654 4 TATCAAC 9010 0.0 26.213575 2 AACGCAG 9170 0.0 26.015312 6 GTACATG 5830 0.0 25.513525 1 TACATGG 5930 0.0 24.842865 2 ACATGGG 5880 0.0 24.245916 3 GAGTACT 5585 0.0 23.48446 12-13 ACGCAGA 10270 0.0 23.22985 7 CGCAGAG 10345 0.0 22.876707 8 GTGTAGC 610 0.0 21.813375 1 GCAGAGT 10755 0.0 21.386003 9 GTACTTT 6190 0.0 21.38106 14-15 AGAGTAC 8605 0.0 21.260216 10-11 AGTACTT 6005 0.0 20.694424 12-13 CATGGGG 2680 0.0 20.393633 4 CATGGGA 2770 0.0 19.55945 4 >>END_MODULE