FastQCFastQC Report
Thu 26 May 2016
SRR936749_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936749_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1178380
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70630.5993822026850422No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60080.5098525093772807No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38220.3243435903528573No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14980.12712367827016752No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13750.11668561923997352No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12420.10539893752439791No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53800.037.4509541
GTATCAA82300.032.5655631
ATCAACG103700.025.55833
TATCAAC104650.025.4646112
TCAACGC104950.025.2980734
CAACGCA106050.024.9919455
AACGCAG108900.024.2942716
GTACATG59800.024.1574861
TACATGG61300.023.5573562
GAGTACT70100.022.9050112-13
ACATGGG60500.022.689193
ACGCAGA120200.022.0103667
AGTACTT72050.021.98840112-13
CGCAGAG120600.021.897988
AGAGTAC102100.021.84540610-11
GTACTTT74900.021.34199714-15
GCAGAGT127400.020.5072149
GTAAGGT4200.020.355484
CATGGGG30100.019.7243024
TACTTTT79850.019.66203314-15
Produced by FastQC (version 0.11.5)