##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936749_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1178380 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.78919363872435 31.0 31.0 34.0 30.0 34.0 2 31.933033486651166 31.0 31.0 34.0 30.0 34.0 3 32.0221176530491 31.0 31.0 34.0 30.0 34.0 4 35.79630849131859 37.0 35.0 37.0 35.0 37.0 5 35.5675605492286 37.0 35.0 37.0 33.0 37.0 6 35.67171201140549 37.0 35.0 37.0 33.0 37.0 7 35.603238344167416 37.0 35.0 37.0 33.0 37.0 8 35.5819345202736 37.0 35.0 37.0 33.0 37.0 9 37.19711807736044 39.0 37.0 39.0 33.0 39.0 10-11 37.063580508834164 39.0 37.0 39.0 33.0 39.0 12-13 36.95722135474126 39.0 37.0 39.0 33.0 39.0 14-15 38.026313667916966 40.0 37.0 41.0 33.0 41.0 16-17 37.9528912574891 40.0 37.0 41.0 33.0 41.0 18-19 38.11925100561788 40.0 37.0 41.0 33.0 41.0 20-21 38.15611941818429 40.0 37.0 41.0 33.0 41.0 22-23 38.062603744123294 40.0 37.0 41.0 33.0 41.0 24-25 37.92610533104771 40.0 37.0 41.0 33.0 41.0 26-27 37.69834179127277 40.0 37.0 41.0 32.0 41.0 28-29 37.590742375125174 39.0 37.0 41.0 32.0 41.0 30-31 37.52386539146964 39.0 36.5 41.0 32.0 41.0 32-33 37.336867139632375 39.0 36.0 41.0 32.0 41.0 34-35 37.137829902068944 39.0 36.0 41.0 31.0 41.0 36-37 36.861833619036304 39.0 35.0 41.0 31.0 41.0 38-39 36.67391036847197 39.0 35.0 41.0 30.0 41.0 40-41 36.40665786927816 39.0 35.0 40.0 30.0 41.0 42-43 36.12026553403825 39.0 35.0 40.0 30.0 41.0 44-45 35.79809526638266 38.0 35.0 40.0 29.0 41.0 46-47 35.359705697652714 38.0 35.0 40.0 27.0 41.0 48-49 34.93744971910589 38.0 34.0 40.0 25.5 41.0 50-51 34.5892865629084 38.0 34.0 40.0 24.0 41.0 52-53 34.30505185084608 38.0 33.5 40.0 23.5 41.0 54-55 34.024607087696666 38.0 33.0 40.0 23.0 41.0 56-57 33.702743172830495 37.5 33.0 40.0 22.5 41.0 58-59 33.355769785637904 37.0 32.5 40.0 21.5 41.0 60-61 33.00470646141312 36.5 32.0 39.5 20.0 41.0 62-63 32.60536456830564 36.0 31.5 39.0 18.5 40.0 64-65 32.18637663572022 35.5 31.0 39.0 17.5 40.0 66-67 31.72307362650418 35.0 31.0 38.5 14.0 40.0 68-69 31.44040886640982 35.0 30.5 38.0 13.0 40.0 70-71 31.008833313532136 35.0 30.0 37.5 9.0 39.5 72-73 30.55791298222984 34.0 29.5 37.0 7.5 39.0 74-75 30.020087323274325 34.0 29.0 36.0 7.0 39.0 76-77 28.627438941597788 32.5 27.0 35.0 3.5 37.0 78-79 29.40506330725233 34.0 29.0 35.0 2.0 37.5 80-81 29.214604796415415 34.0 29.0 35.0 2.0 37.0 82-83 28.858033910962508 34.0 29.0 35.0 2.0 37.0 84-85 28.41377229756106 33.5 28.0 35.0 2.0 36.0 86-87 28.01471723892123 33.0 27.0 35.0 2.0 36.0 88-89 27.581853052495802 33.0 26.5 35.0 2.0 35.0 90-91 27.06940800081468 33.0 26.0 35.0 2.0 35.0 92-93 26.533955939510175 32.0 25.0 34.0 2.0 35.0 94-95 25.909735399446696 32.0 24.0 34.0 2.0 35.0 96-97 25.093561923997353 32.0 19.5 34.0 2.0 35.0 98-99 24.09619986761486 31.0 8.5 34.0 2.0 35.0 100-101 20.97450695021979 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3110315499452554 1101 2 0.4015896657746225 1101 3 0.2888743534712397 1101 4 0.12644442245091625 1101 5 0.30079806680272014 1101 6 0.24710122657278788 1101 7 0.21611645151774184 1101 8 0.24707259650976 1101 9 0.3036067552514723 1101 10-11 0.2576166060724816 1101 12-13 0.22755802978949902 1101 14-15 0.4174087813613454 1101 16-17 0.3796489420492932 1101 18-19 0.4115546812532429 1101 20-21 0.39592477034167217 1101 22-23 0.1974972503989889 1101 24-25 0.202686844356343 1101 26-27 0.22166304740495235 1101 28-29 0.17369156284306797 1101 30-31 0.2920664880780848 1101 32-33 0.42717990266463346 1101 34-35 0.34181359195246586 1101 36-37 0.2241151671034345 1101 38-39 0.25630647291996667 1101 40-41 0.33412671010673733 1101 42-43 0.5018794981373418 1101 44-45 0.45187970285485335 1101 46-47 0.4043194085614701 1101 48-49 0.4761329699871695 1101 50-51 0.5820232241031675 1101 52-53 0.6265398566807932 1101 54-55 0.557213056172067 1101 56-57 0.5209205216116715 1101 58-59 0.3650327006227627 1101 60-61 0.44547783489822734 1101 62-63 0.4563474272887831 1101 64-65 0.4861850688757947 1101 66-67 0.4296761110704814 1101 68-69 0.4462848466730627 1101 70-71 0.39669056405852743 1101 72-73 0.33794717491512216 1101 74-75 0.4319739692727431 1101 76-77 0.4158905486614124 1101 78-79 0.19242123782354525 1101 80-81 0.5497037148785218 1101 82-83 0.7832021826662405 1101 84-85 0.6242023398888747 1101 86-87 0.5431679784220478 1101 88-89 0.5483680942567908 1101 90-91 0.612751681068108 1101 92-93 0.5600558448872768 1101 94-95 0.7919669571301213 1101 96-97 0.763533357988937 1101 98-99 0.8884319260569207 1101 100-101 1.1136324238105075 1102 1 0.15464598386746786 1102 2 0.17322831929772065 1102 3 0.21462337047296032 1102 4 0.13135479885157508 1102 5 0.05397088989995069 1102 6 -0.0017680946507425688 1102 7 0.14540518534211344 1102 8 0.2409219347165532 1102 9 0.19541118012825365 1102 10-11 0.14617609823618238 1102 12-13 0.048041429579058104 1102 14-15 0.3789974311924311 1102 16-17 0.25473316608059804 1102 18-19 0.10878255686935034 1102 20-21 0.07267870694981582 1102 22-23 -0.031225797910998665 1102 24-25 0.15433821224690547 1102 26-27 -0.04855302761674096 1102 28-29 -0.15920103651821194 1102 30-31 0.12459219371394425 1102 32-33 0.14928098073222884 1102 34-35 0.1408688982971924 1102 36-37 0.17471373460264772 1102 38-39 0.19707217320241455 1102 40-41 0.2158848233038313 1102 42-43 0.26571646810282346 1102 44-45 0.2739454760545357 1102 46-47 0.12118763602635596 1102 48-49 0.21661450069549204 1102 50-51 0.24893217907906973 1102 52-53 0.27692647959710825 1102 54-55 0.40660837377743064 1102 56-57 0.366641133193518 1102 58-59 0.3184804632632918 1102 60-61 0.5111760405802315 1102 62-63 0.3476730443163447 1102 64-65 0.3800650969350805 1102 66-67 0.5444359687434037 1102 68-69 0.3862954150583633 1102 70-71 0.3500235281906292 1102 72-73 0.10040713965640435 1102 74-75 0.023629329151649614 1102 76-77 -0.045344475613568136 1102 78-79 0.28394218537851046 1102 80-81 0.4172054685843811 1102 82-83 0.3268827266842571 1102 84-85 0.338986887894265 1102 86-87 0.17518453248233712 1102 88-89 0.0017881668048325139 1102 90-91 0.21347093122040306 1102 92-93 0.34202982543037663 1102 94-95 0.5134138567627637 1102 96-97 0.6227321220878075 1102 98-99 0.6370462676788229 1102 100-101 1.0865618971449464 1103 1 0.13809184948262043 1103 2 0.17537328531511776 1103 3 0.19639611305722227 1103 4 0.09341787967667159 1103 5 0.014448776439664357 1103 6 0.12806550948661766 1103 7 0.07693841497732024 1103 8 0.18738075929498876 1103 9 0.09728765386356031 1103 10-11 0.07683561336488509 1103 12-13 0.2565291542308117 1103 14-15 0.041386962965674456 1103 16-17 0.25241172335564954 1103 18-19 0.17594835333039782 1103 20-21 0.16790244226199746 1103 22-23 0.03292812940784984 1103 24-25 0.016524764845634365 1103 26-27 0.19871746597313233 1103 28-29 0.1426081903032781 1103 30-31 0.01497972417939053 1103 32-33 -0.003657449330972895 1103 34-35 0.03449864644945677 1103 36-37 0.12483460566690496 1103 38-39 0.11055400291291306 1103 40-41 0.21606625374352717 1103 42-43 0.40159672153575343 1103 44-45 0.40032075594040606 1103 46-47 0.2982389765062621 1103 48-49 0.5848941438286275 1103 50-51 0.7322746355357665 1103 52-53 0.37573857462950855 1103 54-55 0.3874956367413418 1103 56-57 0.33855656803212497 1103 58-59 0.5276047424952708 1103 60-61 0.20396848415722246 1103 62-63 0.36173978594948863 1103 64-65 0.3996490577411542 1103 66-67 0.7433376024842637 1103 68-69 0.6257328196990208 1103 70-71 0.37075303955449 1103 72-73 0.41626453161550714 1103 74-75 0.4196876444864195 1103 76-77 0.3420794845980524 1103 78-79 0.29516176946315653 1103 80-81 0.4862431565785137 1103 82-83 0.5633838065978658 1103 84-85 0.47345594688330905 1103 86-87 0.37285021005381935 1103 88-89 0.23552258731724507 1103 90-91 0.2946353554976575 1103 92-93 0.3545404759544759 1103 94-95 0.4725666199545522 1103 96-97 0.571223215277417 1103 98-99 0.895528295538611 1103 100-101 1.1967966792798066 1104 1 0.2221803908652049 1104 2 0.26616188342353553 1104 3 0.07462239457005992 1104 4 0.06841419795220816 1104 5 -0.044134959610687474 1104 6 0.16053354666384934 1104 7 0.1756324936735041 1104 8 0.0534253054338123 1104 9 0.10557289751190524 1104 10-11 0.12864259009638346 1104 12-13 0.09428510226122455 1104 14-15 0.13140444433886955 1104 16-17 0.160256455489737 1104 18-19 0.0020881197727078415 1104 20-21 0.02363509179455292 1104 22-23 0.07329789726829716 1104 24-25 0.03405079600120331 1104 26-27 0.19539573234452945 1104 28-29 0.26797670365021276 1104 30-31 0.08113144940821115 1104 32-33 0.06891377158339651 1104 34-35 0.01650387722539648 1104 36-37 0.24601243023137442 1104 38-39 0.24401856017873058 1104 40-41 0.37895563019576173 1104 42-43 0.22509450977387502 1104 44-45 0.3842074160981426 1104 46-47 0.4492537688570124 1104 48-49 0.16833395172442778 1104 50-51 0.10957741782858221 1104 52-53 0.43965058414199376 1104 54-55 0.4032363724317136 1104 56-57 0.3534760746264993 1104 58-59 0.16673301923003692 1104 60-61 0.13239473785258582 1104 62-63 0.2212005224034712 1104 64-65 0.41420243729148964 1104 66-67 0.08460175666943215 1104 68-69 0.2994631701991892 1104 70-71 0.2477251484462677 1104 72-73 0.17577156234563418 1104 74-75 0.2240871688621624 1104 76-77 0.31142026501455433 1104 78-79 0.2676112207498562 1104 80-81 0.48657996618699784 1104 82-83 0.2316358874539901 1104 84-85 0.09487376533883563 1104 86-87 0.20024244461512453 1104 88-89 0.3925849530024834 1104 90-91 0.33796243444473717 1104 92-93 0.3136528803660035 1104 94-95 0.2859543246311631 1104 96-97 0.6102890390564468 1104 98-99 0.6948354495383278 1104 100-101 1.1293703274051268 1105 1 0.044932371346078526 1105 2 0.05823995992491504 1105 3 -0.01080929621240756 1105 4 0.08602928203578131 1105 5 0.03296867397332193 1105 6 -0.029677550205988723 1105 7 0.03021346561475724 1105 8 -0.02233716039999223 1105 9 0.05303649218120654 1105 10-11 0.031228410934282635 1105 12-13 -0.14855544007397015 1105 14-15 0.14410500180756003 1105 16-17 0.1423043422046888 1105 18-19 0.10701963296819628 1105 20-21 0.17851024042713703 1105 22-23 0.05809567974741725 1105 24-25 0.07230264522214469 1105 26-27 0.1982170489589521 1105 28-29 0.20279301694453267 1105 30-31 0.2112251072367073 1105 32-33 0.24795773594246384 1105 34-35 0.1574091075479842 1105 36-37 0.06264885563712141 1105 38-39 0.2027662631297673 1105 40-41 0.36189685662687765 1105 42-43 0.47999166374950164 1105 44-45 0.6750176759928266 1105 46-47 0.6788150518071134 1105 48-49 0.5757250445792508 1105 50-51 0.7122929543728915 1105 52-53 0.8111281850191148 1105 54-55 0.9817780744870888 1105 56-57 0.6253737073148571 1105 58-59 0.6764192307592936 1105 60-61 0.6403954173158084 1105 62-63 0.6809589269002814 1105 64-65 0.4742581069488274 1105 66-67 0.579536667181042 1105 68-69 0.8655919284801463 1105 70-71 0.8174087347766985 1105 72-73 0.7789596346775802 1105 74-75 0.43494738474615957 1105 76-77 0.3947602900311509 1105 78-79 0.5110879877402965 1105 80-81 0.7612491139239204 1105 82-83 0.6299422481563077 1105 84-85 0.5987895582260947 1105 86-87 0.32752598153004797 1105 88-89 0.25452651916521774 1105 90-91 0.3981621039785317 1105 92-93 0.4443951167265503 1105 94-95 0.5963736615368269 1105 96-97 0.775104425810003 1105 98-99 0.9988867490117421 1105 100-101 0.9417917644698477 1106 1 0.04637314789698621 1106 2 0.06561833778787474 1106 3 0.08287073887732177 1106 4 -0.0292843323967773 1106 5 0.013602577514717495 1106 6 -0.04152033297388158 1106 7 0.008766777207718235 1106 8 -0.10753177488976462 1106 9 -0.03130090316940226 1106 10-11 0.032110421728184235 1106 12-13 0.12395967330017754 1106 14-15 0.2413702915838627 1106 16-17 0.09475728573692521 1106 18-19 0.01657204824747538 1106 20-21 0.029464719134921324 1106 22-23 -0.03482630282213961 1106 24-25 -0.10297578413118202 1106 26-27 -0.08613750984494573 1106 28-29 -0.030923633512209392 1106 30-31 0.11556793768085782 1106 32-33 0.06645707801008882 1106 34-35 0.08369016863080958 1106 36-37 0.06843818378327882 1106 38-39 0.36063904379989964 1106 40-41 0.4605280712272588 1106 42-43 0.44201513953949956 1106 44-45 0.3620325700561793 1106 46-47 0.13179291095237744 1106 48-49 0.4645549738276813 1106 50-51 0.3303288832017799 1106 52-53 0.14299261280865494 1106 54-55 0.25529915692887784 1106 56-57 0.5383592900140997 1106 58-59 0.5052852986121508 1106 60-61 0.32812354821574985 1106 62-63 0.2458067442811398 1106 64-65 0.551073618896492 1106 66-67 0.5010043331420952 1106 68-69 0.4778113536951665 1106 70-71 0.4338436948239952 1106 72-73 0.6014818994905973 1106 74-75 0.610216278847652 1106 76-77 0.4676267774350862 1106 78-79 0.4430954443072217 1106 80-81 0.43636878133433754 1106 82-83 0.6764528227505799 1106 84-85 0.5857772768217409 1106 86-87 0.5483910195757069 1106 88-89 0.6068969827691575 1106 90-91 0.773410497288598 1106 92-93 0.4591037194307468 1106 94-95 0.5267696605451597 1106 96-97 0.2286734705684772 1106 98-99 0.718689560003984 1106 100-101 0.9974924147740225 1107 1 -0.05561824762559198 1107 2 0.0805988875470014 1107 3 0.09448613934618422 1107 4 -0.03746695856572302 1107 5 -0.013473188258011248 1107 6 -0.04838910181227618 1107 7 0.07070484557982581 1107 8 -0.08746968869041893 1107 9 -0.03610871933242521 1107 10-11 0.05427371358484834 1107 12-13 0.02309053091411073 1107 14-15 0.11428518711932867 1107 16-17 0.05233225289050125 1107 18-19 0.011996089260435383 1107 20-21 0.17339328136281296 1107 22-23 -0.020043797734167867 1107 24-25 -0.034338009444830675 1107 26-27 0.061069255232204966 1107 28-29 0.08434158533520275 1107 30-31 -0.1459395847964089 1107 32-33 0.01369024904339966 1107 34-35 -0.11964052410690584 1107 36-37 -0.11607826519526299 1107 38-39 0.03935584314039886 1107 40-41 -0.08005176492700627 1107 42-43 -0.2626841545842069 1107 44-45 0.03487565427185757 1107 46-47 0.32268704238963153 1107 48-49 0.10644673516936365 1107 50-51 -0.051158287367819355 1107 52-53 -0.06285791659855988 1107 54-55 0.10145225995074725 1107 56-57 8.322400102329652E-4 1107 58-59 0.1273049678292466 1107 60-61 0.2539292519022709 1107 62-63 0.14086722036120847 1107 64-65 0.23365470450331927 1107 66-67 0.3052524718442129 1107 68-69 0.18953720834647925 1107 70-71 0.09130369946113959 1107 72-73 -0.06833094024457509 1107 74-75 0.005213960275892049 1107 76-77 -0.29384854265662597 1107 78-79 -0.2450647045966079 1107 80-81 0.190167052710283 1107 82-83 0.0345095375920188 1107 84-85 -0.12215674693073808 1107 86-87 0.11580349349177155 1107 88-89 0.28126001958292335 1107 90-91 0.0800818655035549 1107 92-93 0.034499164725335874 1107 94-95 0.1330600745390278 1107 96-97 0.13579212991875877 1107 98-99 0.13421465210280203 1107 100-101 0.2567667018132056 1108 1 -0.16181350286704443 1108 2 0.02994641940430043 1108 3 0.06364201666685432 1108 4 0.011481559016438325 1108 5 -0.09562543933694201 1108 6 0.01161434567106312 1108 7 0.10240764697924476 1108 8 0.08671121480558952 1108 9 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-1.0940853140403917 2316 46-47 -1.4218645011980229 2316 48-49 -1.3226893317421045 2316 50-51 -1.0767983322474564 2316 52-53 -1.3895230532774718 2316 54-55 -1.0674328889680638 2316 56-57 -0.7082150876111442 2316 58-59 -0.9758289570993597 2316 60-61 -0.9442806624184108 2316 62-63 -0.8729919187656776 2316 64-65 -1.1498962270892257 2316 66-67 -1.7222320582795483 2316 68-69 -1.210176343945104 2316 70-71 -0.7345581936437178 2316 72-73 -0.8084327694299382 2316 74-75 -0.839760029932183 2316 76-77 -0.45252527324278446 2316 78-79 -0.5286394359987554 2316 80-81 -1.1661330968885188 2316 82-83 -0.8813836352625977 2316 84-85 -0.6969688984335249 2316 86-87 -0.778512601979461 2316 88-89 -0.7989410888082062 2316 90-91 -0.7413218132670103 2316 92-93 -0.5207928929382 2316 94-95 -0.6917775767597618 2316 96-97 -0.8864989417526665 2316 98-99 -0.6101456430148353 2316 100-101 -0.8685902953308187 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 8.0 4 15.0 5 57.0 6 111.0 7 258.0 8 601.0 9 1152.0 10 1924.0 11 3140.0 12 4326.0 13 5809.0 14 7716.0 15 10177.0 16 12602.0 17 15845.0 18 13751.0 19 8908.0 20 7996.0 21 8311.0 22 8921.0 23 9944.0 24 11719.0 25 13847.0 26 16991.0 27 20818.0 28 25204.0 29 31016.0 30 37895.0 31 45943.0 32 55833.0 33 70315.0 34 91463.0 35 121247.0 36 167854.0 37 212284.0 38 127237.0 39 7140.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.617551080006386 19.87459633466785 15.515010747430294 21.992841837895476 2 15.663709904643577 21.13185257604921 39.630710267873816 23.5737272514334 3 19.121884072607166 25.348689386179384 30.496095782120797 25.03333075909266 4 12.934507266362575 17.614256921608145 37.22210671475549 32.22912909727379 5 13.38685915822847 38.03960039783701 33.832864324678454 14.740676119256058 6 31.02165685093094 37.144214939153755 17.049932958807855 14.784195251107452 7 27.497666287615203 33.98394405879258 20.680001357796296 17.838388295795923 8 26.49595206979073 34.732004955956484 19.4920993227991 19.279943651453692 9 26.4869094263502 16.67799590450174 20.5181407829649 36.31695388618317 10-11 24.898546641388897 27.175667487571857 26.816061793624097 21.10972407741515 12-13 26.083628409643 24.59784405119795 27.873647228050675 21.444880311108378 14-15 22.81687738361347 25.728853200809944 26.815959156724865 24.638310258851725 16-17 21.444626007715048 29.01171259896813 27.40950985130435 22.13415154201247 18-19 21.307345677964665 26.885215295575282 30.803857838727744 21.00358118773231 20-21 22.356073592559277 25.85715134336971 31.03120385614148 20.75557120792953 22-23 21.876170856467585 25.82616056672105 31.040749517867035 21.256919058944334 24-25 21.70294559433794 26.08230012644585 31.04462095946095 21.170133319755255 26-27 21.527716190957097 26.319720111768586 30.97203451502235 21.180529182251963 28-29 21.330583067875573 26.55968015221794 31.008040749870258 21.101696030036226 30-31 21.520050816022323 26.206615332979453 30.902472359028604 21.370861491969617 32-33 21.395784201490695 26.317086976409076 30.818362883864843 21.468765938235386 34-35 21.844390137712686 26.37440045353995 30.576901288438762 21.204308120308603 36-37 21.970352163540085 26.15145077663039 30.59455234160364 21.283644718225883 38-39 21.69998353535062 26.545046092531326 30.543706854556042 21.21126351756201 40-41 21.75626190748968 26.526004472336435 30.35210102218771 21.365632597986174 42-43 21.763384522427476 26.648964337359217 29.93522441054468 21.652426729668626 44-45 21.885512456252908 26.787647959374183 29.69748736383664 21.62935222053627 46-47 22.108794323051274 27.09606709892696 29.137175247872182 21.65796333014958 48-49 22.38416868003595 27.223977467236605 28.715610422166836 21.676243430560614 50-51 22.126949252292473 27.597733646138707 28.50356234064302 21.771754760925806 52-53 22.458971109092275 27.567358158232746 28.096447012826868 21.87722371984811 54-55 22.141383830124525 27.50840054975387 28.26849171147172 22.081723908649884 56-57 22.265875115573618 27.731685055901778 28.070290823316952 21.932149005207656 58-59 22.40668654162922 27.375215022145056 28.16630981689066 22.051788619335063 60-61 22.1987663980963 27.427996041882718 28.56056581388362 21.812671746137354 62-63 22.13558253286153 27.56053026545098 28.469751946740917 21.834135254946574 64-65 22.134630726664742 27.482474156807495 28.353334578106697 22.029560538421062 66-67 22.216097748301095 27.648732424846127 28.0067383357414 22.12843149111138 68-69 22.166819212355055 27.487205469070563 28.20041490729932 22.145560411275056 70-71 22.255309091850293 27.449348872752793 28.11573011920184 22.17961191619508 72-73 22.311055516046853 27.439785057562954 28.050632997330194 22.19852642906 74-75 22.104830777247543 27.84098093947292 27.807161000730712 22.247027282548824 76-77 22.144358645996963 27.73835801629916 27.89917706474261 22.21810627296127 78-79 22.20464088022693 27.799878217735657 27.801872561437456 22.19360834059996 80-81 22.18080255890067 27.93451006667552 27.60217207826237 22.28251529616144 82-83 22.234447793392846 27.80223599237385 27.677948186115152 22.285368028118153 84-85 22.105028653629493 27.849927543765475 27.543128963610634 22.501914838994395 86-87 22.06457263392306 27.73202276298431 27.483278049658132 22.720126553434497 88-89 22.010690367211907 27.702148173106544 27.45852560179995 22.8286358578816 90-91 22.00259372769424 27.70446611731016 27.663260135780288 22.62968001921531 92-93 22.00301944819966 27.656168186150087 27.625065663239024 22.71574670241123 94-95 21.874205718276784 27.58823926279624 27.575933863144435 22.96162115578254 96-97 21.991096989855862 27.566489344774602 27.509121745332838 22.933291920036694 98-99 21.883452103016896 27.57077595344602 27.304721678373905 23.241050265163185 100-101 21.697213292280118 27.587778407682883 27.315947952487807 23.3990603475492 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 596.0 1 462.0 2 465.0 3 588.5 4 485.5 5 435.0 6 656.0 7 1229.0 8 1864.0 9 2141.0 10 1935.5 11 1663.0 12 1667.0 13 1812.0 14 1997.5 15 2110.5 16 2231.0 17 2338.5 18 2491.5 19 2749.5 20 3046.5 21 3400.0 22 3826.0 23 4517.0 24 5484.0 25 6654.5 26 8223.0 27 10073.5 28 11814.0 29 13761.5 30 15750.5 31 17908.0 32 19970.5 33 21649.0 34 23641.0 35 25786.5 36 28317.5 37 30563.5 38 31796.5 39 32701.0 40 33967.0 41 35764.5 42 37588.5 43 39096.0 44 40340.0 45 41589.5 46 41711.5 47 42317.5 48 45369.0 49 45107.5 50 42014.0 51 40074.5 52 38411.5 53 37661.5 54 36706.5 55 33762.0 56 30373.0 57 27544.0 58 25589.5 59 23343.0 60 19515.0 61 15816.5 62 12951.0 63 10149.0 64 7401.5 65 5500.0 66 4219.0 67 3459.5 68 2791.0 69 2053.0 70 1555.5 71 1243.0 72 1066.5 73 945.5 74 779.5 75 606.5 76 440.5 77 291.5 78 228.5 79 162.5 80 104.5 81 96.0 82 67.0 83 40.0 84 23.0 85 10.0 86 6.5 87 8.0 88 6.0 89 4.0 90 3.5 91 1.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03598160186018093 2 0.005431185186442404 3 0.0033096284729883397 4 4.243113426908128E-4 5 6.788981483053005E-4 6 0.0 7 0.0 8 0.0 9 0.0011032094909961133 10-11 5.091736112289753E-4 12-13 0.001060778356727032 14-15 0.0018669699078395765 16-17 0.0028428859960284458 18-19 0.0 20-21 0.0 22-23 0.0019094010421086577 24-25 8.486226853816256E-4 26-27 0.004455269098253534 28-29 0.005897927663402298 30-31 9.759160881888696E-4 32-33 2.5458680561448767E-4 34-35 0.007934622108318199 36-37 0.012644478012186221 38-39 0.008316502316739931 40-41 0.002121556713454064 42-43 3.3944907415265024E-4 44-45 0.001357796296610601 46-47 4.243113426908128E-5 48-49 5.94035879767138E-4 50-51 9.759160881888696E-4 52-53 0.0028428859960284458 54-55 0.002927748264566608 56-57 0.0015699519679560072 58-59 0.0011032094909961133 60-61 0.003564215278602828 62-63 0.005473616320711486 64-65 0.010183472224579507 66-67 0.004030957755562722 68-69 0.003776370949948234 70-71 4.243113426908128E-5 72-73 0.001527520833686926 74-75 0.006873843751591167 76-77 0.0031399039359120144 78-79 0.004030957755562722 80-81 0.005219029515096997 82-83 0.00564334085778781 84-85 0.007001137154398412 86-87 0.005388754052173323 88-89 0.010438059030193994 90-91 0.012983927086338871 92-93 0.0016123831022250886 94-95 0.003012610533104771 96-97 0.0012305028938033571 98-99 0.0041582511583699655 100-101 0.006110083334747704 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1178380.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.536959399715606 #Duplication Level Percentage of deduplicated Percentage of total 1 77.34338075101188 46.047897196097395 2 13.288920794045433 15.823638755622389 3 4.229361723019759 7.554100115704158 4 1.8014269818289401 4.2900594031480725 5 0.9543143645410023 2.8408487788121524 6 0.5545950331756104 1.9811341184076152 7 0.36089068156475296 1.5040433699239475 8 0.24261680906552668 1.1555733688818255 9 0.1817135862159691 0.9736806964425186 >10 0.9550536736493054 10.351206024221453 >50 0.06007853235317291 2.4433711880820286 >100 0.026079455104867415 2.9545233088246583 >500 5.700416086580698E-4 0.20541303823076268 >1k 7.125520108225872E-4 0.7654838624196368 >5k 2.850208043290349E-4 1.1090267751814535 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7063 0.5993822026850422 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6008 0.5098525093772807 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3822 0.3243435903528573 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1498 0.12712367827016752 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1375 0.11668561923997352 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1242 0.10539893752439791 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 8.486226853816256E-5 0.0 0.0 0.0 0.0 3 8.486226853816256E-5 0.0 0.0 0.0 0.0 4 8.486226853816256E-5 0.0 0.0 0.0 0.0 5 8.486226853816256E-5 0.0 0.0 0.0 0.0 6 8.486226853816256E-5 0.0 0.0 0.0 0.0 7 3.3944907415265024E-4 0.0 0.0 0.0 0.0 8 3.3944907415265024E-4 0.0 0.0 0.0 0.0 9 3.3944907415265024E-4 0.0 0.0 0.0 0.0 10-11 3.3944907415265024E-4 0.0 0.0 0.0 0.0 12-13 5.94035879767138E-4 0.0 0.0 0.0 0.0 14-15 7.63760416843463E-4 0.0 0.0 0.0 0.0 16-17 8.061915511125443E-4 0.0 0.0 0.0 0.0 18-19 8.910538196507068E-4 0.0 0.0 8.486226853816256E-5 0.0 20-21 9.334849539197882E-4 0.0 0.0 1.2729340280724383E-4 0.0 22-23 9.334849539197882E-4 0.0 0.0 2.5458680561448767E-4 0.0 24-25 0.001188071759534276 0.0 0.0 4.243113426908128E-4 0.0 26-27 0.0012305028938033571 0.0 0.0 5.516047454980567E-4 0.0 28-29 0.0016972453707632513 0.0 0.0 0.001060778356727032 0.0 30-31 0.001951832176377739 0.0 0.0 0.0024610057876067143 0.0 32-33 0.001951832176377739 0.0 0.0 0.004073388889831803 0.0 34-35 0.002163987847723145 0.0 0.0 0.005940358797671379 0.0 36-37 0.002715592593221202 0.0 0.0 0.008401364585278093 0.0 38-39 0.003055041667373852 0.0 0.0 0.011159388312768376 0.0 40-41 0.003055041667373852 0.0 0.0 0.014723603591371204 0.0 42-43 0.0032247662044501773 0.0 0.0 0.02028208218062085 0.0 44-45 0.0038188020842173155 0.0 0.0 0.029447207182742408 0.0 46-47 0.004200682292639047 0.0 0.0 0.04086118230112527 0.0 48-49 0.00432797569544629 0.0 0.0 0.06228890510701132 0.0 50-51 0.00432797569544629 0.0 0.0 0.08329231657020655 0.0 52-53 0.004370406829715371 0.0 0.0 0.10404114122778729 0.0 54-55 0.004412837963984453 0.0 0.0 0.1460055330199087 0.0 56-57 0.004582562501060778 0.0 0.0 0.1890731343030262 0.0 58-59 0.004582562501060778 0.0 0.0 0.2429182436904903 0.0 60-61 0.004582562501060778 0.0 0.0 0.30512228652896345 0.0 62-63 0.004624993635329859 0.0 0.0 0.3820923640930769 0.0 64-65 0.004667424769598941 0.0 0.0 0.450533783669105 0.0 66-67 0.004667424769598941 0.0 0.0 0.5419304468847062 0.0 68-69 0.004752287038137104 0.0 0.0 0.6629864729543951 0.0 70-71 0.005049304978020672 0.0 0.0 0.7503521784144334 0.0 72-73 0.005388754052173323 0.0 0.0 0.8691169232335918 0.0 74-75 0.005558478589249648 0.0 0.0 1.0104550314839016 0.0 76-77 0.00564334085778781 0.0 0.0 1.1293470697058674 0.0 78-79 0.0056857719920568916 0.0 0.0 1.2390315517914425 0.0 80-81 0.005982789931940461 0.0 0.0 1.395305419304469 0.0 82-83 0.006025221066209542 0.0 0.0 1.6182385987542218 0.0 84-85 0.006322239006093111 0.0 0.0 1.8592050102683344 0.0 86-87 0.006661688080245761 0.0 0.0 2.1579626266569356 0.0 88-89 0.006788981483053005 0.0 0.0 2.473607834484631 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5380 0.0 37.450954 1 GTATCAA 8230 0.0 32.565563 1 ATCAACG 10370 0.0 25.5583 3 TATCAAC 10465 0.0 25.464611 2 TCAACGC 10495 0.0 25.298073 4 CAACGCA 10605 0.0 24.991945 5 AACGCAG 10890 0.0 24.294271 6 GTACATG 5980 0.0 24.157486 1 TACATGG 6130 0.0 23.557356 2 GAGTACT 7010 0.0 22.90501 12-13 ACATGGG 6050 0.0 22.68919 3 ACGCAGA 12020 0.0 22.010366 7 AGTACTT 7205 0.0 21.988401 12-13 CGCAGAG 12060 0.0 21.89798 8 AGAGTAC 10210 0.0 21.845406 10-11 GTACTTT 7490 0.0 21.341997 14-15 GCAGAGT 12740 0.0 20.507214 9 GTAAGGT 420 0.0 20.35548 4 CATGGGG 3010 0.0 19.724302 4 TACTTTT 7985 0.0 19.662033 14-15 >>END_MODULE