FastQCFastQC Report
Thu 26 May 2016
SRR936748_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936748_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1227890
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61870.5038724967220191No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49290.4014203226673399No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30590.2491265504238979No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12800.10424386549283732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37400.041.433181
GTATCAA64500.038.9850651
ATCAACG80550.030.8065223
TATCAAC81350.030.7932382
TCAACGC81100.030.4816074
CAACGCA81300.030.3481485
AACGCAG83500.029.548566
GTACATG62200.028.3521391
TACATGG63250.027.2802542
ACGCAGA94200.025.9903287
CGCAGAG95250.025.6570458
ACATGGG64300.025.2104783
GAGTACT51100.023.95097712-13
GCAGAGT98900.023.8448129
AGTACTT53650.021.61658112-13
CATGGGG29400.021.5060044
AGAGTAC84450.021.33511410-11
GTACTTT57900.021.21671314-15
ACCGCGC1200.004737424719.8105838
CAGAGTA97600.019.62956210-11
Produced by FastQC (version 0.11.5)