##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936748_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1227890 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.337497658585054 31.0 31.0 34.0 30.0 34.0 2 31.389413546816083 31.0 31.0 34.0 28.0 34.0 3 31.350285449022305 31.0 31.0 34.0 28.0 34.0 4 35.03804412447369 37.0 35.0 37.0 32.0 37.0 5 35.06597170756338 37.0 35.0 37.0 32.0 37.0 6 35.11549080129328 37.0 35.0 37.0 32.0 37.0 7 34.991425942063216 37.0 35.0 37.0 32.0 37.0 8 34.97537971642411 37.0 35.0 37.0 32.0 37.0 9 36.45996954124555 39.0 37.0 39.0 32.0 39.0 10-11 36.341785909161246 39.0 36.0 39.0 32.0 39.0 12-13 36.2354074876414 39.0 35.0 39.0 32.0 39.0 14-15 37.20207876927086 39.0 36.5 41.0 31.0 41.0 16-17 37.126162767023104 39.0 36.0 41.0 31.0 41.0 18-19 37.04248222560653 39.0 36.0 41.0 31.0 41.0 20-21 37.04035703523931 39.0 36.0 41.0 31.0 41.0 22-23 36.995728851932995 39.0 36.0 41.0 31.0 41.0 24-25 37.093400060265985 39.0 36.0 41.0 31.0 41.0 26-27 37.00175626481199 39.0 36.0 41.0 31.0 41.0 28-29 36.869827101776224 39.0 36.0 41.0 30.5 41.0 30-31 36.640532946762335 39.0 36.0 41.0 30.0 41.0 32-33 36.43889395629902 39.0 35.0 40.5 30.0 41.0 34-35 36.231804966242905 39.0 35.0 40.0 30.0 41.0 36-37 36.0033015986774 38.0 35.0 40.0 29.5 41.0 38-39 35.795181978841754 38.0 35.0 40.0 28.5 41.0 40-41 35.55507659480898 38.0 35.0 40.0 27.5 41.0 42-43 35.24229817003152 38.0 34.0 40.0 26.5 41.0 44-45 35.03699069134858 38.0 34.0 40.0 25.5 41.0 46-47 34.82079665116582 38.0 34.0 40.0 24.0 41.0 48-49 34.55152090170944 38.0 34.0 40.0 23.5 41.0 50-51 33.553692513173004 37.5 32.5 39.5 22.5 40.5 52-53 33.662850092434994 37.5 33.0 39.5 22.5 40.5 54-55 34.107047048188356 38.0 33.5 40.0 23.0 41.0 56-57 33.96162563421805 38.0 33.0 40.0 22.0 41.0 58-59 33.75177581053677 38.0 33.0 40.0 21.5 41.0 60-61 33.472512195717854 37.0 33.0 40.0 20.0 41.0 62-63 33.139762926646526 37.0 32.5 40.0 18.5 41.0 64-65 32.78335518653951 36.0 32.0 39.5 18.0 41.0 66-67 32.426473869809186 36.0 31.5 39.0 15.0 41.0 68-69 31.9846305450814 35.0 31.0 39.0 12.0 40.5 70-71 31.609033382469114 35.0 31.0 38.5 9.0 40.0 72-73 31.18924130011646 35.0 31.0 37.5 7.5 40.0 74-75 30.7765573463421 35.0 30.0 37.0 6.5 39.0 76-77 30.31346252514476 34.5 30.0 36.5 2.0 39.0 78-79 29.85860011890316 34.0 29.0 36.0 2.0 38.5 80-81 29.420687113666535 34.0 29.0 35.5 2.0 37.5 82-83 29.016013649431137 34.0 29.0 35.0 2.0 37.0 84-85 28.64236372965005 34.0 28.5 35.0 2.0 36.5 86-87 28.275826010473253 34.0 28.0 35.0 2.0 36.0 88-89 27.92714982612449 34.0 27.0 35.0 2.0 36.0 90-91 27.567102916385018 34.0 26.5 35.0 2.0 35.5 92-93 27.130062546319294 33.0 26.0 35.0 2.0 35.0 94-95 26.631192533533135 33.0 25.0 35.0 2.0 35.0 96-97 26.022406323041967 33.0 24.0 35.0 2.0 35.0 98-99 25.28415533964769 32.5 19.0 35.0 2.0 35.0 100-101 22.801832818900714 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4285135907719173 1101 2 0.40415561996708504 1101 3 0.3932446641348015 1101 4 0.569422032954229 1101 5 0.5207787796310797 1101 6 0.4439825907864332 1101 7 0.4356417419347238 1101 8 0.4421487788284466 1101 9 0.5971866452232888 1101 10-11 0.7046996202491655 1101 12-13 0.6721075138871839 1101 14-15 0.8400811107386659 1101 16-17 0.8490287866620392 1101 18-19 0.925524155877028 1101 20-21 0.8007179349476132 1101 22-23 0.6519594843941121 1101 24-25 0.6710042588760956 1101 26-27 0.7117261911819668 1101 28-29 0.7334609722016339 1101 30-31 0.5411828941680525 1101 32-33 0.583184110515127 1101 34-35 0.7289060725182779 1101 36-37 0.6649979725437518 1101 38-39 0.6293992086957871 1101 40-41 0.5755092371961652 1101 42-43 0.6704031320161405 1101 44-45 0.694269125470548 1101 46-47 0.8548122820484778 1101 48-49 1.0302545490963482 1101 50-51 0.8060183893866437 1101 52-53 0.7996591397300037 1101 54-55 0.8635429368001439 1101 56-57 0.8553568653150236 1101 58-59 0.7722021817270672 1101 60-61 0.6295338422122185 1101 62-63 0.7438847005903071 1101 64-65 0.6686539733917698 1101 66-67 0.7587628925497185 1101 68-69 0.7875059593278841 1101 70-71 0.626292862219362 1101 72-73 0.6273246689913421 1101 74-75 0.4028727506822456 1101 76-77 0.4711232050414864 1101 78-79 0.460419245009529 1101 80-81 0.5778653407449568 1101 82-83 0.5845765342871303 1101 84-85 0.6035346952010521 1101 86-87 0.4875756255696828 1101 88-89 0.49312541234602847 1101 90-91 0.44565240075152346 1101 92-93 0.5583312995843457 1101 94-95 0.6731944422893505 1101 96-97 0.6563244713240657 1101 98-99 0.5814117742956952 1101 100-101 0.8126432096347536 1102 1 0.10729271422077247 1102 2 0.3235320878138843 1102 3 0.3841966565271733 1102 4 0.3141294148137348 1102 5 0.3232772445280361 1102 6 0.3032396141983824 1102 7 0.35637834694838944 1102 8 0.02470171829504153 1102 9 0.2239176050964531 1102 10-11 0.3665188774426298 1102 12-13 0.32419165374191294 1102 14-15 0.45897328047762187 1102 16-17 0.23510965995564703 1102 18-19 0.22481318595495026 1102 20-21 0.3919053142351032 1102 22-23 0.04755892652266169 1102 24-25 0.20536851365298503 1102 26-27 0.17064282160222888 1102 28-29 0.05266236788994405 1102 30-31 0.015528406206961165 1102 32-33 0.22738043790564433 1102 34-35 -0.06816805163601458 1102 36-37 0.1507307474387929 1102 38-39 -0.06505829213254088 1102 40-41 0.07319402813121201 1102 42-43 0.20718329755484888 1102 44-45 0.548194140170601 1102 46-47 0.36050950047630437 1102 48-49 0.5485570160753639 1102 50-51 0.3972840184095716 1102 52-53 0.22213025840363088 1102 54-55 0.45798120068162973 1102 56-57 0.48447689987775533 1102 58-59 0.24213369128801787 1102 60-61 0.34516008852857283 1102 62-63 0.17702754209460636 1102 64-65 0.4287645964642621 1102 66-67 0.25557378406263354 1102 68-69 0.3054558375160319 1102 70-71 0.13717754655408143 1102 72-73 0.35633029154703166 1102 74-75 0.12778866210832973 1102 76-77 0.3073288395007019 1102 78-79 0.15089557466384207 1102 80-81 0.43969716572275175 1102 82-83 0.2962933494245874 1102 84-85 0.3048539937114363 1102 86-87 -0.003290013124850333 1102 88-89 0.0936241115936376 1102 90-91 0.3045747703713886 1102 92-93 0.3121969560810278 1102 94-95 0.29989803468289367 1102 96-97 0.19825809269790895 1102 98-99 0.327842111704836 1102 100-101 0.6828437707477946 1103 1 0.300978688408744 1103 2 0.45016093865424267 1103 3 0.500002447508507 1103 4 0.5193193833198322 1103 5 0.4058358001439686 1103 6 0.39819823047445624 1103 7 0.4822937086941437 1103 8 0.2820683575525109 1103 9 0.39392116711614733 1103 10-11 0.17367457622652438 1103 12-13 0.2534246583909834 1103 14-15 0.5817841507959614 1103 16-17 0.7801503546064623 1103 18-19 0.6599709986198405 1103 20-21 0.67444346894613 1103 22-23 0.3865929382493789 1103 24-25 0.28832274373449707 1103 26-27 0.37951919564521575 1103 28-29 0.3617429951418174 1103 30-31 0.3793586055688323 1103 32-33 0.5579022263162514 1103 34-35 0.47550765427733666 1103 36-37 0.3811983052524326 1103 38-39 0.21018681036165532 1103 40-41 0.45417604413669466 1103 42-43 0.5664205804256071 1103 44-45 0.6754488538783576 1103 46-47 0.9390144613958213 1103 48-49 0.9763112264070628 1103 50-51 0.9734085572528102 1103 52-53 0.9884016756235638 1103 54-55 1.0408136221686632 1103 56-57 1.1232864745215991 1103 58-59 0.865501652806266 1103 60-61 0.8383025902058137 1103 62-63 0.9404083789711919 1103 64-65 0.8519059505905986 1103 66-67 0.8489632669078375 1103 68-69 0.959931652270388 1103 70-71 0.9670132460216259 1103 72-73 0.8870335177927977 1103 74-75 0.7605084852498472 1103 76-77 0.6633821433757099 1103 78-79 0.481097649425017 1103 80-81 0.6527234419255024 1103 82-83 0.6648724562393333 1103 84-85 0.9128024521273659 1103 86-87 0.6983595031268273 1103 88-89 0.5260242753434561 1103 90-91 0.8449609660122697 1103 92-93 0.8035153130160211 1103 94-95 0.7859982904809399 1103 96-97 0.7926895951675839 1103 98-99 1.0624437012934287 1103 100-101 1.4104834158914166 1104 1 0.2778241775374717 1104 2 0.34397822445129833 1104 3 0.48678585517341944 1104 4 0.5323290935838827 1104 5 0.4234859005252787 1104 6 0.4136265240819341 1104 7 0.482306901987684 1104 8 0.31588080393721185 1104 9 0.6249135610420709 1104 10-11 0.5201391665494981 1104 12-13 0.6410512017256167 1104 14-15 0.6577790654830693 1104 16-17 0.6697236105092017 1104 18-19 0.5502412534132191 1104 20-21 0.7245718088143605 1104 22-23 0.4165699257769475 1104 24-25 0.5490324699427731 1104 26-27 0.2544547730735758 1104 28-29 0.28236680774539735 1104 30-31 0.25012299692571105 1104 32-33 0.4110765888836809 1104 34-35 0.43161146135658157 1104 36-37 0.2527714174324771 1104 38-39 0.16370683721983426 1104 40-41 0.3873055551202569 1104 42-43 0.4442426820555099 1104 44-45 0.5214811533066026 1104 46-47 0.732707702162763 1104 48-49 0.7369927414238333 1104 50-51 0.5964375634104258 1104 52-53 0.6245064988671132 1104 54-55 0.7523259778284199 1104 56-57 0.6582851944914196 1104 58-59 0.718095229749558 1104 60-61 0.663493135049535 1104 62-63 0.7570912144401518 1104 64-65 0.5392232094396476 1104 66-67 0.5511339286160677 1104 68-69 0.5754860962207822 1104 70-71 0.5236787224847852 1104 72-73 0.7479582385441077 1104 74-75 0.5884595162355595 1104 76-77 0.6218401112267422 1104 78-79 0.450949710498179 1104 80-81 0.6743437411830833 1104 82-83 0.7019446286077446 1104 84-85 0.6534224414829062 1104 86-87 0.48715017215593903 1104 88-89 0.4822889283205356 1104 90-91 0.6600858700100041 1104 92-93 0.6853685272391736 1104 94-95 0.5281325611556724 1104 96-97 0.5888391745541597 1104 98-99 0.16891400122471012 1104 100-101 0.7586966790252028 1105 1 0.371142554817844 1105 2 0.32926426805412845 1105 3 0.4863928648839462 1105 4 0.4168105524414045 1105 5 0.38588700150408783 1105 6 0.4152960510634358 1105 7 0.4594671062820339 1105 8 0.3321949004413227 1105 9 0.3665207792162164 1105 10-11 0.5239378706814364 1105 12-13 0.5241264401255918 1105 14-15 0.797440148475836 1105 16-17 0.8709570606791246 1105 18-19 0.43243597540516276 1105 20-21 0.5570415937089805 1105 22-23 0.5559773982282294 1105 24-25 0.37543241831167506 1105 26-27 0.3816895455331064 1105 28-29 0.5626119100622518 1105 30-31 0.26450547192219176 1105 32-33 0.33630299007434417 1105 34-35 0.19687127126684345 1105 36-37 0.27017752396223926 1105 38-39 0.08718402257308355 1105 40-41 0.2360102021622552 1105 42-43 0.1895723999993777 1105 44-45 0.1543273241207359 1105 46-47 0.3332951878005872 1105 48-49 0.4488911663114834 1105 50-51 0.6000885553094335 1105 52-53 0.6117956718482915 1105 54-55 0.5384195755711971 1105 56-57 0.2127960079358715 1105 58-59 0.28792539249662497 1105 60-61 0.3130512406484769 1105 62-63 0.47640342992367124 1105 64-65 0.4868776300804569 1105 66-67 0.5455926746346549 1105 68-69 0.4250938331299956 1105 70-71 0.6464341025430578 1105 72-73 0.4634090290090782 1105 74-75 0.4563972050042189 1105 76-77 0.504537509309845 1105 78-79 0.5764598100759173 1105 80-81 0.6439727425938493 1105 82-83 0.4787138727089548 1105 84-85 0.5276211594316216 1105 86-87 0.7078239156778103 1105 88-89 0.5943616081931289 1105 90-91 0.6149541016082445 1105 92-93 0.756081327390433 1105 94-95 0.9322674734797509 1105 96-97 0.9344088377642059 1105 98-99 1.084853014706976 1105 100-101 1.1769963612393752 1106 1 0.26922258043222413 1106 2 0.07019710897310105 1106 3 0.2467248719555002 1106 4 0.3939315017191376 1106 5 0.33560222094452286 1106 6 0.24425382459975253 1106 7 0.3280357372238214 1106 8 0.5115028015665075 1106 9 0.4828702333235597 1106 10-11 0.574450882813764 1106 12-13 0.6107740478170811 1106 14-15 0.4464726053171191 1106 16-17 0.2852875882994539 1106 18-19 0.46776184109803864 1106 20-21 0.2443874554548131 1106 22-23 0.15308070311801458 1106 24-25 -0.06738737845699916 1106 26-27 -0.14715130029345147 1106 28-29 0.041970494812581194 1106 30-31 0.168805541064458 1106 32-33 0.30157194105786544 1106 34-35 0.2842749930803308 1106 36-37 0.04708952835643032 1106 38-39 0.3936845338946071 1106 40-41 0.32932275831446844 1106 42-43 0.2682550190696773 1106 44-45 0.14411434938870826 1106 46-47 0.41519530276168126 1106 48-49 0.4217906077910385 1106 50-51 0.17150657894477206 1106 52-53 0.29754443011673004 1106 54-55 0.553947258008094 1106 56-57 0.6236604518374804 1106 58-59 0.4203107453774848 1106 60-61 0.3898484909669335 1106 62-63 0.441959266096525 1106 64-65 0.23763702190455405 1106 66-67 0.12255915788801275 1106 68-69 0.3162879241576455 1106 70-71 0.20694031085586317 1106 72-73 0.2717978417632736 1106 74-75 0.358821048458406 1106 76-77 0.11035311890033483 1106 78-79 0.3868023057942054 1106 80-81 0.5037174544527865 1106 82-83 0.5814384367055716 1106 84-85 0.733825538993667 1106 86-87 0.6314608746326975 1106 88-89 0.7708148032971387 1106 90-91 1.0562116420481864 1106 92-93 1.1531300844167909 1106 94-95 1.1367520945014853 1106 96-97 1.1899215876803666 1106 98-99 1.286737555407754 1106 100-101 1.7217031828619973 1107 1 0.14009303457818945 1107 2 0.2857314173927037 1107 3 0.28889457767187565 1107 4 0.3431696577514387 1107 5 0.28402626320442437 1107 6 0.30636237927026144 1107 7 0.315382281403366 1107 8 0.46462881342666407 1107 9 0.49187636693073244 1107 10-11 0.6446577879787583 1107 12-13 0.4963309293912772 1107 14-15 0.29581183172400927 1107 16-17 0.3264905339111053 1107 18-19 0.7134773360884665 1107 20-21 0.4507953634599602 1107 22-23 0.37811955854805746 1107 24-25 0.3555944520038139 1107 26-27 0.4440722294448278 1107 28-29 0.5417079893774499 1107 30-31 0.4538517512723317 1107 32-33 0.5577853406304527 1107 34-35 0.2550582537910131 1107 36-37 0.2962823530536767 1107 38-39 0.3896521266156938 1107 40-41 0.5292096612591521 1107 42-43 0.4397589554947885 1107 44-45 0.49219424355391794 1107 46-47 0.44363377703845686 1107 48-49 0.3088704834536671 1107 50-51 0.41467449916162025 1107 52-53 0.3158920566975425 1107 54-55 0.288587799023702 1107 56-57 0.4299776971725109 1107 58-59 0.4327352316994464 1107 60-61 0.3775095791040002 1107 62-63 0.28482836955373614 1107 64-65 0.3249183444693813 1107 66-67 0.20624750958516103 1107 68-69 0.27627376967955897 1107 70-71 0.36203707937926666 1107 72-73 0.44192333555103147 1107 74-75 0.2297817027098361 1107 76-77 0.3570087765284846 1107 78-79 0.5823792626739284 1107 80-81 0.44298040394997784 1107 82-83 0.4260791627470333 1107 84-85 0.4471395371249045 1107 86-87 0.5244407623220049 1107 88-89 0.4537383043564134 1107 90-91 0.507007484517171 1107 92-93 0.788663837758822 1107 94-95 0.5928789944857549 1107 96-97 0.9729995853305411 1107 98-99 1.0875884430693041 1107 100-101 1.236507564274298 1108 1 0.060193212766282755 1108 2 0.1735706646064692 1108 3 0.24081138581334116 1108 4 0.2718747775022621 1108 5 0.13042719109203205 1108 6 0.20805914148503746 1108 7 0.3527980395913701 1108 8 0.3607423487065091 1108 9 -0.0066279755271452245 1108 10-11 0.1652179346448719 1108 12-13 0.043342888104021426 1108 14-15 0.4945645150683333 1108 16-17 0.5757356898607568 1108 18-19 0.5368447636790918 1108 20-21 0.4650983486951361 1108 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80-81 -4.151230341906821 2316 82-83 -3.139844427975614 2316 84-85 -3.4119006408496695 2316 86-87 -3.9219195292397124 2316 88-89 -5.178349527808425 2316 90-91 -3.9897437307689607 2316 92-93 -3.5860801366145516 2316 94-95 -3.2404423063115964 2316 96-97 -4.777167533224702 2316 98-99 -6.264248256700203 2316 100-101 -6.573504040438177 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2795.0 3 1098.0 4 1151.0 5 1243.0 6 1382.0 7 1690.0 8 2166.0 9 2672.0 10 3527.0 11 4392.0 12 5830.0 13 7285.0 14 8099.0 15 9858.0 16 12287.0 17 15303.0 18 11782.0 19 8633.0 20 8818.0 21 8929.0 22 9994.0 23 11478.0 24 12942.0 25 15058.0 26 17466.0 27 20399.0 28 24061.0 29 29073.0 30 35296.0 31 42732.0 32 52808.0 33 66929.0 34 86901.0 35 118553.0 36 161743.0 37 209648.0 38 167257.0 39 26608.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.21095956282372 18.713492234904827 14.714274653311218 22.361273548960238 2 15.296471473795517 20.051843608914755 40.94105437061826 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21.965326628050207 46-47 21.996079443792464 27.091540182443495 28.836407584737035 22.075972789027006 48-49 22.153273436252935 27.17817806893811 28.521831052444696 22.146717442364263 50-51 21.9751513895342 27.459787250417488 28.451011523562446 22.114049836485865 52-53 22.318856277722965 27.489737438873576 28.05554752458597 22.135858758817495 54-55 21.93911664653727 27.377393233427448 28.22763295379305 22.455857166242225 56-57 22.066644405714005 27.60449073998664 28.047700877950255 22.281163976349095 58-59 22.259002549556726 27.30261295969697 28.106857415805784 22.331527074940517 60-61 22.131059421422986 27.227366757883765 28.524278407610108 22.117295413083138 62-63 22.038489416632054 27.42721134973572 28.356137050339203 22.178162183293022 64-65 21.915091673595903 27.378777723393565 28.322389075885525 22.383741527125007 66-67 21.949513228426742 27.56900884935572 28.04686265663099 22.434615265586544 68-69 21.974209358890775 27.411891613664064 28.190815072054896 22.42308395539026 70-71 22.04754677735206 27.244414998560508 28.18621272949906 22.52182549458837 72-73 22.018965140161452 27.33467000794592 28.12881437939322 22.517550472499405 74-75 21.874250377636585 27.659980866684613 27.9146844733838 22.551084282295005 76-77 22.00512379796433 27.501965208678687 27.952395110805185 22.540515882551798 78-79 21.987157008764953 27.59438889900287 27.939309596566186 22.479144495665988 80-81 21.96809050025107 27.713787824673275 27.769621507900403 22.548500167175252 82-83 22.015518270658383 27.656996863605528 27.810029458163626 22.517455407572466 84-85 22.0345450206744 27.533316447407536 27.674742862913703 22.757395669004364 86-87 21.97562706733606 27.55024214529369 27.741181232507937 22.73294955486231 88-89 21.954505973936282 27.49472564604473 27.71934125243154 22.831427127587446 90-91 21.89061189252584 27.412915468753816 27.96107767361931 22.735394965101033 92-93 22.020828675530645 27.402377546112756 27.814666896349788 22.76212688200681 94-95 21.78180806730314 27.451774921533833 27.82892563969217 22.937491371470855 96-97 21.840461815701325 27.32478797813095 27.871229166878773 22.96352103928895 98-99 21.849587287157394 27.379116640559005 27.634206005332317 23.137090066951284 100-101 21.8725397358748 27.273889890134505 27.68374214701001 23.169828226980684 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 421.0 1 310.0 2 336.5 3 473.0 4 437.5 5 416.0 6 633.0 7 1163.0 8 1815.5 9 2132.5 10 1885.5 11 1614.5 12 1633.0 13 1711.5 14 1841.5 15 1982.5 16 2071.5 17 2115.5 18 2204.0 19 2452.0 20 2696.5 21 3043.0 22 3629.0 23 4398.5 24 5345.5 25 6505.0 26 8216.5 27 10267.5 28 12049.0 29 13808.5 30 15873.5 31 18282.5 32 20623.5 33 22423.0 34 24511.5 35 26795.5 36 29299.0 37 31674.5 38 32979.5 39 34082.5 40 35559.0 41 37426.0 42 39156.0 43 40623.5 44 41924.5 45 43293.5 46 43862.5 47 44725.5 48 47477.0 49 47522.0 50 44499.0 51 42245.5 52 41030.5 53 40368.5 54 39020.5 55 35753.5 56 32227.0 57 29177.5 58 26913.5 59 24424.5 60 20650.0 61 16898.0 62 13640.5 63 10618.0 64 7813.0 65 5897.0 66 4625.5 67 3699.0 68 2863.0 69 2140.0 70 1642.5 71 1301.0 72 1083.0 73 925.5 74 785.0 75 639.0 76 442.5 77 305.5 78 224.0 79 159.0 80 110.5 81 78.5 82 47.0 83 29.5 84 20.0 85 16.0 86 16.5 87 11.5 88 7.0 89 6.0 90 5.5 91 3.0 92 3.0 93 3.0 94 2.0 95 2.0 96 2.0 97 1.5 98 0.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07533248092255822 2 0.02866706301053026 3 0.013111923706520942 4 0.020523011018902346 5 0.024839358574465138 6 0.0304587544486884 7 0.07842722067937681 8 0.05578675614265122 9 0.07117901440682797 10-11 0.06116183045712564 12-13 0.040394497878474454 14-15 0.03766624046127911 16-17 0.04870143090993493 18-19 0.04418148205458144 20-21 0.016776747102753505 22-23 0.012134637467525591 24-25 0.01991220711953025 26-27 0.022151821417227928 28-29 0.005456514834390702 30-31 6.515241593302331E-4 32-33 0.008388373551376753 34-35 0.009284219270455822 36-37 0.003542662616358143 38-39 0.007085325232716286 40-41 0.0035833828763162825 42-43 0.0017916914381581413 44-45 2.0360129979069789E-4 46-47 2.4432155974883743E-4 48-49 4.886431194976749E-4 50-51 0.0011808875387860475 52-53 3.664823396232562E-4 54-55 8.144051991627914E-5 56-57 0.0024432155974883746 58-59 0.002402495337530235 60-61 0.004072025995813957 62-63 0.001628810398325583 64-65 8.144051991627914E-5 66-67 3.257620796651166E-4 68-69 0.0033797815765255843 70-71 0.002076733257865118 72-73 0.017631872561874436 74-75 0.015351538004218618 76-77 0.023210548176139555 78-79 0.017631872561874436 80-81 0.011116630968572103 82-83 0.00382770443606512 84-85 0.0035019423564000033 86-87 9.365659790372101E-4 88-89 0.0011808875387860475 90-91 0.004886431194976749 92-93 0.009080617970665126 94-95 0.010709428368990708 96-97 0.010383666289325591 98-99 0.02288478609647444 100-101 0.020563731278860485 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1227890.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.55396151484541 #Duplication Level Percentage of deduplicated Percentage of total 1 78.58429079825846 48.371744114674215 2 12.607928846523588 15.521359340016444 3 3.9923843689593905 7.372412213981902 4 1.7199404911692493 4.234766032050252 5 0.8793906190890157 2.7064988161960675 6 0.504295053900546 1.8624815003952657 7 0.34042528730021654 1.4668167523210405 8 0.2263364500065897 1.1145524106489884 9 0.16866096614319273 0.9343575557131257 >10 0.8997424090694577 10.030126053558943 >50 0.05053094428492394 2.0986409547949494 >100 0.024750231215283837 2.629495433682564 >500 5.294136320547067E-4 0.22069888663976805 >1k 6.617670400683834E-4 0.9320026811217965 >5k 1.3235340801367668E-4 0.504047254204594 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6187 0.5038724967220191 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4929 0.4014203226673399 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3059 0.2491265504238979 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1280 0.10424386549283732 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 8.144051991627914E-5 0.0 0.0 0.0 0.0 3 8.144051991627914E-5 0.0 0.0 0.0 0.0 4 8.144051991627914E-5 0.0 0.0 0.0 0.0 5 1.628810398325583E-4 0.0 0.0 0.0 0.0 6 2.4432155974883743E-4 0.0 0.0 0.0 0.0 7 4.072025995813957E-4 0.0 0.0 0.0 0.0 8 4.072025995813957E-4 0.0 0.0 0.0 0.0 9 4.072025995813957E-4 0.0 0.0 0.0 0.0 10-11 4.072025995813957E-4 0.0 0.0 0.0 0.0 12-13 5.293633794558145E-4 0.0 0.0 0.0 0.0 14-15 9.365659790372101E-4 0.0 0.0 0.0 0.0 16-17 0.0010180064989534893 0.0 0.0 0.0 0.0 18-19 0.0013030483186604663 0.0 0.0 4.072025995813957E-5 0.0 20-21 0.0014659293584930247 0.0 0.0 8.144051991627914E-5 0.0 22-23 0.0017916914381581413 0.0 0.0 2.4432155974883743E-4 0.0 24-25 0.0018731319580744202 0.0 0.0 3.257620796651166E-4 0.0 26-27 0.0018731319580744202 0.0 0.0 4.072025995813957E-4 0.0 28-29 0.0019138522180325598 0.0 0.0 0.0011808875387860477 0.0 30-31 0.0019545724779906994 0.0 0.0 0.002280334557655816 0.0 32-33 0.0019545724779906994 0.0 0.0 0.0034205018364837237 0.0 34-35 0.002076733257865118 0.0 0.0 0.00480499067506047 0.0 36-37 0.0022803345576558162 0.0 0.0 0.006311640293511634 0.0 38-39 0.0025246561174046534 0.0 0.0 0.008469814071293031 0.0 40-41 0.002565376377362793 0.0 0.0 0.011157351228530243 0.0 42-43 0.00285041819706977 0.0 0.0 0.016573145802962805 0.0 44-45 0.0030947397568186073 0.0 0.0 0.026223847413041883 0.0 46-47 0.003461222096441864 0.0 0.0 0.035304465383707004 0.0 48-49 0.0035019423564000033 0.0 0.0 0.054850190163614004 0.0 50-51 0.0035019423564000033 0.0 0.0 0.07504743910285123 0.0 52-53 0.0035019423564000033 0.0 0.0 0.09601837298129312 0.0 54-55 0.0035833828763162825 0.0 0.0 0.13327741084299083 0.0 56-57 0.0035833828763162825 0.0 0.0 0.1710250918241862 0.0 58-59 0.0035833828763162825 0.0 0.0 0.22127389261253044 0.0 60-61 0.0036241031362744223 0.0 0.0 0.28023682903191655 0.0 62-63 0.0036648233962325617 0.0 0.0 0.35499922631506076 0.0 64-65 0.0036648233962325617 0.0 0.0 0.41933723704892134 0.0 66-67 0.0038277044360651197 0.0 0.0 0.5105913396151122 0.0 68-69 0.003909144955981399 0.0 0.0 0.627254884395182 0.0 70-71 0.0039905854758976785 0.0 0.0 0.7134596747265634 0.0 72-73 0.004031305735855818 0.0 0.0 0.8240559007728705 0.0 74-75 0.004072025995813957 0.0 0.0 0.9568039482364056 0.0 76-77 0.004072025995813957 0.0 0.0 1.0739968563959312 0.0 78-79 0.004072025995813957 0.0 0.0 1.183615796203243 0.0 80-81 0.004560669115311632 0.0 0.0 1.3399408741825407 0.0 82-83 0.004723550155144191 0.0 0.0 1.552948554023569 0.0 84-85 0.00480499067506047 0.0 0.0 1.7965371490931599 0.0 86-87 0.004927151454934888 0.0 0.0 2.0808052838609323 0.0 88-89 0.004967871714893028 0.0 0.0 2.398789793874044 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3740 0.0 41.43318 1 GTATCAA 6450 0.0 38.985065 1 ATCAACG 8055 0.0 30.806522 3 TATCAAC 8135 0.0 30.793238 2 TCAACGC 8110 0.0 30.481607 4 CAACGCA 8130 0.0 30.348148 5 AACGCAG 8350 0.0 29.54856 6 GTACATG 6220 0.0 28.352139 1 TACATGG 6325 0.0 27.280254 2 ACGCAGA 9420 0.0 25.990328 7 CGCAGAG 9525 0.0 25.657045 8 ACATGGG 6430 0.0 25.210478 3 GAGTACT 5110 0.0 23.950977 12-13 GCAGAGT 9890 0.0 23.844812 9 AGTACTT 5365 0.0 21.616581 12-13 CATGGGG 2940 0.0 21.506004 4 AGAGTAC 8445 0.0 21.335114 10-11 GTACTTT 5790 0.0 21.216713 14-15 ACCGCGC 120 0.0047374247 19.810583 8 CAGAGTA 9760 0.0 19.629562 10-11 >>END_MODULE