FastQCFastQC Report
Thu 26 May 2016
SRR936748_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936748_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1227890
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72380.5894664831540285No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61220.49857886292746095No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40510.3299155461808468No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16150.1315264396647908No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13810.1124693580043815No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13080.10652420005049312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55000.038.701031
GTATCAA83800.031.3536661
ATCAACG104450.025.144733
TATCAAC105400.025.0133082
TCAACGC106450.024.6723084
CAACGCA107150.024.555455
AACGCAG109250.024.1249526
GTACATG59150.023.6156441
GAGTACT72800.022.8658312-13
TACATGG60250.022.470182
ACGCAGA123150.021.4037027
AGTACTT75350.021.33564212-13
CGCAGAG124550.021.1631158
ACATGGG61050.021.1598973
GTACTTT80050.020.8525414-15
AGAGTAC108350.020.33852210-11
GCAGAGT131300.019.8942819
CATGGGG29450.019.0294864
ACTTTTT89300.018.8255116-17
TACTTTT85800.018.79089414-15
Produced by FastQC (version 0.11.5)