##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936748_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1227890 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.801365757518997 31.0 31.0 34.0 30.0 34.0 2 31.966617530886317 31.0 31.0 34.0 30.0 34.0 3 32.02972904739024 33.0 31.0 34.0 30.0 34.0 4 35.76602301509093 37.0 35.0 37.0 35.0 37.0 5 35.593330835824055 37.0 35.0 37.0 33.0 37.0 6 35.674313660018406 37.0 35.0 37.0 33.0 37.0 7 35.60907491713427 37.0 35.0 37.0 33.0 37.0 8 35.578554267890446 37.0 35.0 37.0 33.0 37.0 9 37.19152855711831 39.0 37.0 39.0 33.0 39.0 10-11 37.08033700087141 39.0 37.0 39.0 33.0 39.0 12-13 36.97027258142016 39.0 37.0 39.0 33.0 39.0 14-15 38.058135907939636 40.0 37.0 41.0 33.0 41.0 16-17 37.963528491965896 40.0 37.0 41.0 33.0 41.0 18-19 38.12196939465261 40.0 37.0 41.0 33.0 41.0 20-21 38.1464992792514 40.0 37.0 41.0 33.0 41.0 22-23 38.056441130720174 40.0 37.0 41.0 33.0 41.0 24-25 37.92330949840784 40.0 37.0 41.0 33.0 41.0 26-27 37.71023015090928 40.0 37.0 41.0 32.0 41.0 28-29 37.59941037063581 39.0 37.0 41.0 32.0 41.0 30-31 37.52899323229279 39.0 36.5 41.0 32.0 41.0 32-33 37.333129596299344 39.0 36.0 41.0 32.0 41.0 34-35 37.13163638436667 39.0 36.0 41.0 31.0 41.0 36-37 36.90279626025132 39.0 35.0 41.0 31.0 41.0 38-39 36.71593953855802 39.0 35.0 41.0 30.5 41.0 40-41 36.49451416657844 39.0 35.0 40.5 30.0 41.0 42-43 36.174467582601046 39.0 35.0 40.0 30.0 41.0 44-45 35.83134645611578 38.5 35.0 40.0 29.5 41.0 46-47 35.37276873335559 38.0 35.0 40.0 27.5 41.0 48-49 34.9321971023463 38.0 34.0 40.0 25.5 41.0 50-51 34.61592650807483 38.0 34.0 40.0 24.0 41.0 52-53 34.240238946485434 38.0 33.0 40.0 23.0 41.0 54-55 34.054258931989025 38.0 33.0 40.0 23.0 41.0 56-57 33.74477844106556 37.5 33.0 40.0 22.5 41.0 58-59 33.389334956714364 37.0 33.0 40.0 21.5 41.0 60-61 32.973076578520875 36.5 32.0 39.5 20.0 41.0 62-63 32.63271099202697 36.0 31.5 39.0 19.0 40.0 64-65 32.21113576949075 35.5 31.0 39.0 18.0 40.0 66-67 31.793254281735337 35.0 31.0 38.5 15.5 40.0 68-69 31.43340608686446 35.0 30.5 38.0 13.0 40.0 70-71 31.079806415884157 35.0 30.0 37.5 9.5 39.5 72-73 30.621644446978152 34.0 29.5 37.0 7.5 39.0 74-75 30.119281043090176 34.0 29.0 36.0 7.0 39.0 76-77 28.636184837404002 32.5 27.0 35.0 4.0 37.0 78-79 29.410696397885804 34.0 29.0 35.0 2.0 37.5 80-81 29.18074949710479 34.0 29.0 35.0 2.0 37.0 82-83 28.87328873107526 34.0 29.0 35.0 2.0 37.0 84-85 28.495129856909006 33.5 28.0 35.0 2.0 36.0 86-87 28.09437775370758 33.0 27.0 35.0 2.0 36.0 88-89 27.609569261090165 33.0 26.5 35.0 2.0 35.0 90-91 27.09820138611765 33.0 26.0 35.0 2.0 35.0 92-93 26.4157607766168 32.0 25.0 34.0 2.0 35.0 94-95 25.86402242871919 32.0 24.0 34.0 2.0 35.0 96-97 25.148409059443438 32.0 20.5 34.0 2.0 35.0 98-99 24.259890136738633 31.0 10.0 34.0 2.0 35.0 100-101 21.144543485165606 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.23098110314043652 1101 2 0.3171936768420771 1101 3 0.2171576959043371 1101 4 0.1595580068106628 1101 5 0.18117647526430858 1101 6 0.1675245789103883 1101 7 0.21326809471980113 1101 8 0.1725637181694637 1101 9 0.16747222012087803 1101 10-11 0.26451852970735246 1101 12-13 0.2795459846385526 1101 14-15 0.4733961787897485 1101 16-17 0.38586122731511097 1101 18-19 0.34652291400779944 1101 20-21 0.3522799425322063 1101 22-23 0.2928767344014318 1101 24-25 0.3302603828773769 1101 26-27 0.33914434326533893 1101 28-29 0.22569603317680276 1101 30-31 0.31161352601455405 1101 32-33 0.4037658659303176 1101 34-35 0.370819359717828 1101 36-37 0.3770614694438734 1101 38-39 0.40895137240253376 1101 40-41 0.5894572992072895 1101 42-43 0.4844556838983536 1101 44-45 0.502223995655747 1101 46-47 0.5430354247220848 1101 48-49 0.5210640392542629 1101 50-51 0.23434951570136064 1101 52-53 0.5004107083308824 1101 54-55 0.5442211139371835 1101 56-57 0.42283224993105506 1101 58-59 0.3557214981402126 1101 60-61 0.45808953647219397 1101 62-63 0.4222552095010954 1101 64-65 0.5621661994061 1101 66-67 0.3006186294267721 1101 68-69 0.359545487198929 1101 70-71 0.21185908486565808 1101 72-73 0.4259895086053831 1101 74-75 0.49123130153686745 1101 76-77 0.4054632394554254 1101 78-79 0.6302413548249 1101 80-81 0.5604134050927634 1101 82-83 0.45966341713022274 1101 84-85 0.4040530799454203 1101 86-87 0.43091101974082946 1101 88-89 0.4235329054236878 1101 90-91 0.5619445006520927 1101 92-93 0.5062276478910839 1101 94-95 0.35691891550051835 1101 96-97 0.5570836582745144 1101 98-99 0.4218518955211543 1101 100-101 0.9700720839711643 1102 1 0.20142980492551388 1102 2 0.17531269136836514 1102 3 0.18568350011632972 1102 4 0.18079942225671175 1102 5 0.13064601114108143 1102 6 0.126022460322055 1102 7 0.2414374680656266 1102 8 0.17726375066502698 1102 9 0.22617200976326757 1102 10-11 0.16981509354757662 1102 12-13 0.20258252075525007 1102 14-15 0.3923333055923166 1102 16-17 0.32850948532189506 1102 18-19 0.24415060522079557 1102 20-21 0.2719563898401134 1102 22-23 0.22555273453002656 1102 24-25 0.16554907391739704 1102 26-27 0.24996714521167718 1102 28-29 0.2821362786763828 1102 30-31 0.38664957536210665 1102 32-33 0.32044464425666064 1102 34-35 0.3363690169467901 1102 36-37 0.3875821397292398 1102 38-39 0.4148485328538172 1102 40-41 0.5357451663001811 1102 42-43 0.6768692591247785 1102 44-45 0.6889932274877921 1102 46-47 0.7393181770190225 1102 48-49 1.0055080184220344 1102 50-51 0.7309374818220178 1102 52-53 0.8214358941175348 1102 54-55 0.5665779534556208 1102 56-57 0.4023681506173986 1102 58-59 0.6434007385252656 1102 60-61 0.8217026389586195 1102 62-63 0.5627388592919473 1102 64-65 0.47876367240463225 1102 66-67 0.3927234835408342 1102 68-69 0.2762521644122593 1102 70-71 0.4726577476321481 1102 72-73 0.6075949221585581 1102 74-75 0.35916766869259575 1102 76-77 0.39244814296911557 1102 78-79 0.48716164717335175 1102 80-81 0.5271783909799126 1102 82-83 0.3766267641038965 1102 84-85 0.5245441216128057 1102 86-87 0.5362800675465031 1102 88-89 0.41503742863038084 1102 90-91 0.38219994286140846 1102 92-93 0.5383750039667028 1102 94-95 0.3690336750479837 1102 96-97 0.5543566084967892 1102 98-99 0.6591739105860235 1102 100-101 1.435679797455439 1103 1 0.10822452705949104 1103 2 0.21832007774387208 1103 3 0.16875861641097956 1103 4 0.16707113246836514 1103 5 0.26550820058681524 1103 6 0.19838951600757326 1103 7 0.12731285271899395 1103 8 0.23686206444342162 1103 9 0.2816830890552353 1103 10-11 0.18091174798907872 1103 12-13 0.3143043327137107 1103 14-15 0.2269649810898784 1103 16-17 0.3582195238964232 1103 18-19 0.2764750523142254 1103 20-21 0.21097070601236112 1103 22-23 0.19178516757396125 1103 24-25 0.23087062826017046 1103 26-27 0.1383693121567262 1103 28-29 0.15965909337187156 1103 30-31 0.30318762861117676 1103 32-33 0.45344809965992994 1103 34-35 0.36011586016448405 1103 36-37 0.434264266230592 1103 38-39 0.33721481536674247 1103 40-41 0.34452683348273894 1103 42-43 0.38778409592703156 1103 44-45 0.4497524666968147 1103 46-47 0.7456092246841663 1103 48-49 0.7460350942480218 1103 50-51 0.7206549645565303 1103 52-53 0.5588299932100469 1103 54-55 0.6374255215293942 1103 56-57 0.5637011924058797 1103 58-59 0.5798752505516234 1103 60-61 0.6597661156146373 1103 62-63 0.6769121709475101 1103 64-65 0.6677864663465343 1103 66-67 0.49875176103980223 1103 68-69 0.6850344826875379 1103 70-71 0.6398395009759881 1103 72-73 0.676452588137689 1103 74-75 0.597091323189165 1103 76-77 0.7148510765758367 1103 78-79 0.7104816991197005 1103 80-81 0.7044798884117576 1103 82-83 0.7351337379850413 1103 84-85 1.1577442612721924 1103 86-87 1.1371637631270168 1103 88-89 1.0365798889632352 1103 90-91 1.017750391559236 1103 92-93 1.1238294300542542 1103 94-95 0.9841436106956074 1103 96-97 1.051277753844829 1103 98-99 0.8348585277882741 1103 100-101 0.9800813360960241 1104 1 0.21129615036232963 1104 2 0.21725900009319687 1104 3 0.1959191164761087 1104 4 0.16162960065400256 1104 5 0.16182968578113588 1104 6 0.01664656403340814 1104 7 0.0530160833355211 1104 8 0.036112629228668425 1104 9 0.10237303655154051 1104 10-11 0.17341458875781512 1104 12-13 0.1384709937662123 1104 14-15 0.25964673414735984 1104 16-17 0.327464412758367 1104 18-19 0.1641460879260137 1104 20-21 0.060376699023848346 1104 22-23 0.24739349630367258 1104 24-25 0.26631342887638 1104 26-27 0.2433668756080536 1104 28-29 0.20629233328545382 1104 30-31 0.0957555095099778 1104 32-33 0.14291137402204868 1104 34-35 0.18831826549838837 1104 36-37 0.149441831119141 1104 38-39 0.20947832979065595 1104 40-41 0.20489813767630238 1104 42-43 0.1620787038560323 1104 44-45 0.14812963544969904 1104 46-47 0.10724287443197511 1104 48-49 0.4065810551913742 1104 50-51 0.2832502650492259 1104 52-53 0.20626280285383558 1104 54-55 0.34778851675510225 1104 56-57 0.14203932023823285 1104 58-59 0.09625416306492696 1104 60-61 0.13602927522317287 1104 62-63 0.2670998413374974 1104 64-65 0.28135251253541327 1104 66-67 0.21246908097099393 1104 68-69 0.020239860161701984 1104 70-71 0.099622412348495 1104 72-73 0.3521956793432075 1104 74-75 0.16663948937750206 1104 76-77 -0.1206609747960421 1104 78-79 -0.1564237942308786 1104 80-81 0.0750402047813239 1104 82-83 -0.047439661640378716 1104 84-85 0.12118017105925816 1104 86-87 0.28873589844192793 1104 88-89 0.40413676609381 1104 90-91 0.5750561638216567 1104 92-93 0.6303933742988299 1104 94-95 0.5187873749275198 1104 96-97 0.759581612798975 1104 98-99 0.6942152115669238 1104 100-101 1.314501572250368 1105 1 -0.02042328668892779 1105 2 0.10522313465403954 1105 3 0.0884194558354956 1105 4 0.07126455903399886 1105 5 0.1531984433864153 1105 6 0.16146212220687772 1105 7 0.039656231490127425 1105 8 0.13519496508780549 1105 9 0.031366023932221765 1105 10-11 0.10103771561519892 1105 12-13 0.12790282737345393 1105 14-15 0.2152773123672702 1105 16-17 0.1469447626378937 1105 18-19 0.1497816413969275 1105 20-21 0.14178553133668004 1105 22-23 0.09594103812972321 1105 24-25 0.15960005181417358 1105 26-27 0.19190272165523936 1105 28-29 -7.687053481859607E-4 1105 30-31 0.058547631281832935 1105 32-33 0.1565757403168746 1105 34-35 0.23190307019706324 1105 36-37 0.13194300675701243 1105 38-39 0.07298086407807602 1105 40-41 0.10862233424295908 1105 42-43 0.11925594277958851 1105 44-45 0.27344354288215555 1105 46-47 -0.0412012536810451 1105 48-49 0.07128504290656679 1105 50-51 0.08530057144227499 1105 52-53 0.1426532188599694 1105 54-55 0.04005545563647672 1105 56-57 0.06595827879275618 1105 58-59 0.1634586840591581 1105 60-61 0.2913608838836268 1105 62-63 -0.03378285666658343 1105 64-65 0.07690196568200491 1105 66-67 -0.015482860891893324 1105 68-69 0.1061847457733478 1105 70-71 0.15996475324953963 1105 72-73 0.20028932798989274 1105 74-75 -0.055403811668206515 1105 76-77 -0.22857377518969813 1105 78-79 -0.1861862184204206 1105 80-81 -0.11785470046469015 1105 82-83 0.015386668769615142 1105 84-85 0.10668179877231765 1105 86-87 0.10305813714731826 1105 88-89 -0.014171097105570851 1105 90-91 0.15839586014348228 1105 92-93 -0.03201236257159579 1105 94-95 0.26076955492374054 1105 96-97 0.5026486823337137 1105 98-99 -0.044805906282896046 1105 100-101 0.49018792660944044 1106 1 0.07526146323275285 1106 2 0.16490077437730477 1106 3 0.0976636108300859 1106 4 0.19263200255056745 1106 5 0.24956909560083318 1106 6 0.06761668069695048 1106 7 0.06268605650914338 1106 8 0.17835091608374398 1106 9 0.09374414516717877 1106 10-11 0.07729973443128557 1106 12-13 0.12439689369758611 1106 14-15 0.16661223417855098 1106 16-17 0.06241161782418203 1106 18-19 0.22357548152510276 1106 20-21 0.09605227142387207 1106 22-23 0.08509391429606694 1106 24-25 0.13290679090770396 1106 26-27 0.13247021954715876 1106 28-29 -0.054098988693453975 1106 30-31 0.0722687823280026 1106 32-33 0.10007885171872744 1106 34-35 0.08773299817042357 1106 36-37 -0.11303555362102458 1106 38-39 0.24329009284512182 1106 40-41 0.244609364203221 1106 42-43 0.29455538674329773 1106 44-45 0.18712146276269692 1106 46-47 0.2524542881196652 1106 48-49 0.0442826429385903 1106 50-51 0.2935928472439997 1106 52-53 0.13072393580037556 1106 54-55 0.028330425171105844 1106 56-57 -0.09567435497545063 1106 58-59 -0.03527891760136015 1106 60-61 -0.08344987194757181 1106 62-63 0.1932337236757533 1106 64-65 0.34433796078545953 1106 66-67 0.25253708271782926 1106 68-69 0.11923621052666533 1106 70-71 0.4124304477842671 1106 72-73 0.2651659207806034 1106 74-75 0.23587142836309383 1106 76-77 0.2142261828557146 1106 78-79 0.2420648825424685 1106 80-81 0.2055380524324555 1106 82-83 0.4783417345789722 1106 84-85 0.6764530358667962 1106 86-87 0.7293552446269693 1106 88-89 0.4371413983228969 1106 90-91 0.6734208465841505 1106 92-93 0.720446949069558 1106 94-95 0.6432551364846972 1106 96-97 0.8954954252638814 1106 98-99 0.9353484700359509 1106 100-101 1.2981094080479814 1107 1 0.0575637399759259 1107 2 0.14201770146786785 1107 3 0.04981361129265238 1107 4 0.031525362897674825 1107 5 -0.03252132653565809 1107 6 -0.09517138143473147 1107 7 0.004553244948724 1107 8 0.07665607923029683 1107 9 -0.06287793026786659 1107 10-11 -0.012815952986038326 1107 12-13 -0.11204186736927824 1107 14-15 0.1428768859136298 1107 16-17 0.13982528013348627 1107 18-19 -0.011444362498096439 1107 20-21 0.030548492270739303 1107 22-23 0.1461293189938715 1107 24-25 0.1775650944056153 1107 26-27 0.020785142807689283 1107 28-29 0.01789457240671055 1107 30-31 -0.07792364456365419 1107 32-33 -0.06301912872404358 1107 34-35 0.029293737533748754 1107 36-37 -0.06150353952204313 1107 38-39 -0.06012109011769695 1107 40-41 -0.13004486872582532 1107 42-43 -0.29592457033069763 1107 44-45 -0.28645568839417734 1107 46-47 -0.3359371895893446 1107 48-49 -0.01586926329030547 1107 50-51 -0.04087492587729713 1107 52-53 -0.1381706734712722 1107 54-55 -0.29149948980951734 1107 56-57 -0.514400590716896 1107 58-59 -0.3199585453293352 1107 60-61 -0.30096395704097034 1107 62-63 -0.4543139898672095 1107 64-65 -0.5627813716412433 1107 66-67 -0.37971593089082134 1107 68-69 -0.18278612209432055 1107 70-71 -0.250703381418365 1107 72-73 -0.19278579672191754 1107 74-75 -0.12454275201861265 1107 76-77 -0.1947942902554054 1107 78-79 -0.09584321511418281 1107 80-81 0.0980854421801709 1107 82-83 0.003988631232779483 1107 84-85 0.29133327788061436 1107 86-87 0.4104678051072952 1107 88-89 0.19752481815581646 1107 90-91 0.33662752971811827 1107 92-93 0.525327931186812 1107 94-95 0.510509491530815 1107 96-97 0.7055270646854908 1107 98-99 0.8181183996336081 1107 100-101 1.5654440180597327 1108 1 0.060055099490281094 1108 2 0.03623430523086313 1108 3 -0.019462739265527773 1108 4 0.009870131136231919 1108 5 -0.004798345883799016 1108 6 0.10835028876296349 1108 7 0.13081751519599294 1108 8 0.1394924034110261 1108 9 0.11193441101143975 1108 10-11 0.14446356847837905 1108 12-13 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-0.7741480179677644 2316 80-81 -0.39376050927255335 2316 82-83 -0.4590754941622883 2316 84-85 -0.4501677408740967 2316 86-87 -0.8677271005843643 2316 88-89 -0.8236470973704328 2316 90-91 -0.8099581713509068 2316 92-93 -0.667017504203816 2316 94-95 -0.8312038346364723 2316 96-97 -1.0410851079626795 2316 98-99 -1.4179372281171645 2316 100-101 -2.63757026589742 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 6.0 4 21.0 5 45.0 6 102.0 7 264.0 8 589.0 9 1197.0 10 1998.0 11 3110.0 12 4504.0 13 5948.0 14 7830.0 15 10577.0 16 13001.0 17 16451.0 18 14273.0 19 9250.0 20 8353.0 21 8614.0 22 9161.0 23 10303.0 24 12028.0 25 14326.0 26 17248.0 27 21682.0 28 26190.0 29 32147.0 30 39595.0 31 47777.0 32 58884.0 33 73705.0 34 95566.0 35 126461.0 36 174623.0 37 221465.0 38 132988.0 39 7607.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.61675208773879 19.870581373753303 15.451873670217097 22.060792868290807 2 15.674978272750895 21.124995011081506 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25.919626350894625 30.9240241389701 20.758699883540057 22-23 21.891066700654537 25.844355638009624 30.965746903832514 21.298830757503325 24-25 21.69274120645986 26.057220109293176 31.057260829553137 21.192777854693826 26-27 21.533075733087706 26.25478317560103 30.992738246632484 21.219402844678783 28-29 21.347864382843717 26.512609199713154 30.99404774266637 21.145478674776754 30-31 21.552324769347354 26.13479634930117 30.89847537350112 21.414403507850356 32-33 21.401431697879005 26.318097889430703 30.776658225909838 21.503812186780454 34-35 21.866144460909293 26.34553292962561 30.53824574862862 21.25007686083648 36-37 21.914944387842773 26.194885652413635 30.567629220794558 21.322540738949034 38-39 21.74838114365248 26.52053591861699 30.534754001431747 21.196328936298787 40-41 21.804295429585714 26.438681425359757 30.31640139377703 21.4406217512775 42-43 21.793232974011286 26.594663800886366 29.925604006771344 21.686499218331004 44-45 21.887083953649743 26.78414943625862 29.69069150755619 21.63807510253545 46-47 22.139075671143605 27.103137874011868 29.095506054921245 21.662280399923283 48-49 22.440470664119193 27.15444155057245 28.67673766697451 21.728350118333847 50-51 22.214435594111354 27.527173680672146 28.462804236703903 21.795586488512598 52-53 22.562829376270983 27.575538179754034 28.0340079600029 21.827624483972087 54-55 22.14835419677481 27.54291899234892 28.214816687984147 22.093910122892126 56-57 22.27406254696276 27.754507101278282 28.042606447902852 21.92882390385611 58-59 22.44584161875986 27.392251439240706 28.102361030893064 22.05954591110637 60-61 22.306169969343006 27.380641577106186 28.535359809321402 21.777828644229402 62-63 22.249672047891394 27.540972122950784 28.387192416354395 21.82216341280343 64-65 22.10860290598813 27.544002199346306 28.299844212970033 22.047550681695533 66-67 22.222077434476983 27.6495275078165 28.0036828572848 22.124712200421712 68-69 22.207938326145463 27.4888354181623 28.190899995398567 22.11232626029367 70-71 22.260684801313474 27.39229686070972 28.140084323582993 22.20693401439381 72-73 22.276466132959793 27.41674742851558 28.10829960338467 22.198486835139956 74-75 22.118269950047257 27.79517705798012 27.80609034984023 22.28046264213239 76-77 22.2097026272689 27.69769671125126 27.88025113755474 22.212349523925095 78-79 22.204434590884027 27.72568187353035 27.79666497523533 22.273218560350298 80-81 22.200831012520577 27.895001787955774 27.590194019674847 22.3139731798488 82-83 22.227349481893352 27.776845548746664 27.701864467112998 22.29394050224699 84-85 22.150304180728362 27.751628848593967 27.55806114572365 22.540005824954022 86-87 22.090199469022007 27.688193172057968 27.55599846709529 22.66560889182474 88-89 22.047569677131563 27.701830966120305 27.460540225647378 22.790059131100758 90-91 22.00936041509139 27.593384155085655 27.73295928610539 22.66429614371757 92-93 22.08526257433357 27.58123178957393 27.536832260616272 22.796673375476225 94-95 21.845172338504955 27.654122144121068 27.533752407394736 22.96695310997924 96-97 22.002569182762763 27.540164360880137 27.525700994549958 22.93156546180714 98-99 21.906293060106304 27.549219407615166 27.309203240849765 23.235284291428762 100-101 21.692283495623503 27.507959674784026 27.390897245019445 23.408859584573023 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 685.0 1 497.5 2 494.5 3 634.5 4 497.5 5 449.0 6 688.0 7 1290.0 8 1904.5 9 2254.5 10 2087.5 11 1709.5 12 1658.0 13 1815.5 14 2023.5 15 2174.0 16 2328.0 17 2476.0 18 2675.5 19 2793.5 20 3039.0 21 3454.5 22 3965.5 23 4725.0 24 5666.5 25 6925.5 26 8567.0 27 10509.0 28 12430.5 29 14333.0 30 16491.5 31 18806.0 32 20682.0 33 22473.0 34 24590.5 35 26457.5 36 29163.0 37 31829.0 38 33114.0 39 33959.5 40 35167.5 41 37162.5 42 38871.0 43 40275.0 44 41838.0 45 43187.5 46 43320.5 47 43928.5 48 47069.5 49 47165.0 50 43992.5 51 41996.0 52 40475.5 53 39507.0 54 38406.0 55 35339.0 56 31930.0 57 29018.0 58 26782.0 59 24461.5 60 20405.0 61 16614.5 62 13792.0 63 10782.5 64 7820.0 65 5754.0 66 4444.5 67 3572.5 68 2803.0 69 2140.0 70 1639.5 71 1268.0 72 1063.5 73 936.5 74 828.0 75 668.5 76 462.5 77 299.5 78 212.0 79 157.5 80 115.0 81 81.0 82 56.5 83 38.5 84 27.5 85 19.0 86 8.0 87 4.5 88 5.5 89 2.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02003436789940467 2 0.013763447865851175 3 0.009691421870037218 4 0.0 5 0.003176180276734887 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0013030483186604663 14-15 1.2216077987441872E-4 16-17 8.144051991627914E-5 18-19 0.001628810398325583 20-21 0.0 22-23 8.144051991627914E-5 24-25 0.0 26-27 0.001506649618451164 28-29 0.0029725789769441888 30-31 0.0012623280587023267 32-33 0.00952854083020466 34-35 0.0017509711782000017 36-37 8.958457190790706E-4 38-39 0.0013844888385767455 40-41 2.8504181970697703E-4 42-43 0.005578675614265121 44-45 0.0017916914381581413 46-47 3.664823396232562E-4 48-49 6.515241593302331E-4 50-51 1.2216077987441872E-4 52-53 8.144051991627914E-5 54-55 0.001995292737948839 56-57 0.015351538004218618 58-59 0.027119693132120957 60-61 0.022355422717018625 62-63 0.015921621643632575 64-65 0.020563731278860485 66-67 0.003176180276734887 68-69 9.365659790372101E-4 70-71 8.144051991627914E-5 72-73 0.0 74-75 0.002402495337530235 76-77 0.003013299236902328 78-79 0.010831589148865126 80-81 0.01881276010066048 82-83 0.02011580841932095 84-85 0.03359421446546515 86-87 0.012664000846981407 88-89 0.04336707685541864 90-91 0.04837566883026981 92-93 0.03253548770655352 94-95 0.0343678994046698 96-97 0.053058498725455865 98-99 0.055460994062986095 100-101 0.062464878775786103 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1227890.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.935580270583344 #Duplication Level Percentage of deduplicated Percentage of total 1 76.93614602653496 45.34276409856172 2 13.462810341327577 15.868770790779019 3 4.326991453907494 7.650412564856797 4 1.8199642340039457 4.290425928109211 5 0.9967217531928236 2.9371187446366105 6 0.5544952113741131 1.9607698223755872 7 0.3751968727617731 1.5478711788346327 8 0.2650435559205094 1.249639661212322 9 0.18963037386903525 1.005837850880933 >10 0.9798735652172939 10.432863364120601 >50 0.06345767556821982 2.5170755706992285 >100 0.027737013717344973 3.009544735082247 >500 9.659613024843514E-4 0.3322242373760666 >1k 6.899723589173938E-4 0.7681414741779558 >5k 2.7598894356695755E-4 1.0865399782972458 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7238 0.5894664831540285 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6122 0.49857886292746095 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4051 0.3299155461808468 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1615 0.1315264396647908 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1381 0.1124693580043815 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1308 0.10652420005049312 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 8.144051991627914E-5 0.0 0.0 0.0 0.0 6 8.144051991627914E-5 0.0 0.0 0.0 0.0 7 3.257620796651166E-4 0.0 0.0 0.0 0.0 8 3.257620796651166E-4 0.0 0.0 0.0 0.0 9 3.257620796651166E-4 0.0 0.0 0.0 0.0 10-11 3.257620796651166E-4 0.0 0.0 0.0 0.0 12-13 3.257620796651166E-4 0.0 0.0 0.0 0.0 14-15 3.257620796651166E-4 0.0 0.0 0.0 0.0 16-17 3.257620796651166E-4 0.0 0.0 0.0 0.0 18-19 3.664823396232562E-4 0.0 0.0 0.0 0.0 20-21 4.072025995813957E-4 0.0 0.0 0.0 0.0 22-23 4.072025995813957E-4 0.0 0.0 1.628810398325583E-4 0.0 24-25 4.886431194976749E-4 0.0 0.0 2.4432155974883743E-4 0.0 26-27 4.886431194976749E-4 0.0 0.0 3.257620796651166E-4 0.0 28-29 7.736849392046519E-4 0.0 0.0 0.0010994470188697685 0.0 30-31 0.001058726758911629 0.0 0.0 0.002443215597488374 0.0 32-33 0.001058726758911629 0.0 0.0 0.003705543656190701 0.0 34-35 0.001140167278827908 0.0 0.0 0.005212193274641865 0.0 36-37 0.0013844888385767455 0.0 0.0 0.006800283413009308 0.0 38-39 0.0015880901383674433 0.0 0.0 0.009039897710706984 0.0 40-41 0.0016288103983255829 0.0 0.0 0.011849595647818616 0.0 42-43 0.0018324116981162809 0.0 0.0 0.01734683074216746 0.0 44-45 0.002198894037739537 0.0 0.0 0.026997532352246535 0.0 46-47 0.002443215597488374 0.0 0.0 0.03603743006295353 0.0 48-49 0.0025246561174046534 0.0 0.0 0.05554243458290238 0.0 50-51 0.0025246561174046534 0.0 0.0 0.07508815936280938 0.0 52-53 0.0025246561174046534 0.0 0.0 0.09585549194146055 0.0 54-55 0.0025246561174046534 0.0 0.0 0.13364389318261408 0.0 56-57 0.002768977677153491 0.0 0.0 0.17143229442376762 0.0 58-59 0.002768977677153491 0.0 0.0 0.22123317235257228 0.0 60-61 0.002768977677153491 0.0 0.0 0.27970746565246074 0.0 62-63 0.0029318587169860494 0.0 0.0 0.3543069818957724 0.0 64-65 0.0030947397568186073 0.0 0.0 0.4178305874304702 0.0 66-67 0.0030947397568186073 0.0 0.0 0.5091254102566191 0.0 68-69 0.0030947397568186073 0.0 0.0 0.6262368778962284 0.0 70-71 0.003135460016776747 0.0 0.0 0.7113829414686983 0.0 72-73 0.0031761802767348865 0.0 0.0 0.8198209937372241 0.0 74-75 0.0032576207966511657 0.0 0.0 0.9528540830204659 0.0 76-77 0.0032576207966511657 0.0 0.0 1.0692733062407869 0.0 78-79 0.0032576207966511657 0.0 0.0 1.1780778408489359 0.0 80-81 0.0036648233962325617 0.0 0.0 1.3334256325892384 0.0 82-83 0.0038277044360651197 0.0 0.0 1.5453338654113968 0.0 84-85 0.003990585475897678 0.0 0.0 1.7860313220239599 0.0 86-87 0.004194186775688375 0.0 0.0 2.0677340804143696 0.0 88-89 0.004234907035646516 0.0 0.0 2.3813208023520023 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5500 0.0 38.70103 1 GTATCAA 8380 0.0 31.353666 1 ATCAACG 10445 0.0 25.14473 3 TATCAAC 10540 0.0 25.013308 2 TCAACGC 10645 0.0 24.672308 4 CAACGCA 10715 0.0 24.55545 5 AACGCAG 10925 0.0 24.124952 6 GTACATG 5915 0.0 23.615644 1 GAGTACT 7280 0.0 22.86583 12-13 TACATGG 6025 0.0 22.47018 2 ACGCAGA 12315 0.0 21.403702 7 AGTACTT 7535 0.0 21.335642 12-13 CGCAGAG 12455 0.0 21.163115 8 ACATGGG 6105 0.0 21.159897 3 GTACTTT 8005 0.0 20.85254 14-15 AGAGTAC 10835 0.0 20.338522 10-11 GCAGAGT 13130 0.0 19.894281 9 CATGGGG 2945 0.0 19.029486 4 ACTTTTT 8930 0.0 18.82551 16-17 TACTTTT 8580 0.0 18.790894 14-15 >>END_MODULE