FastQCFastQC Report
Thu 26 May 2016
SRR936747_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936747_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2445784
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT258451.0567163739725176No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT149750.612278107960474No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT100040.4090303967971006No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC95350.3898545415294237No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81820.3345348567167011No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT79530.32517180585039396No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64990.26572256585209486No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC62160.2541516339954796No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT61070.24969498532985743No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC59870.2447885831291725No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG55580.22724819526172385No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC49410.20202111061320216No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA48670.1989954959227798No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA40300.16477334057300236No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37660.15397925573149548No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC28460.11636350552624433No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT28430.11624084547122722No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA28110.11493247155104458No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC26420.10802262178507996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGCG2750.027.64731694-95
GTACATG123250.025.7102811
TACATGG129300.023.884132
ACATGGG127750.023.2079123
GAGTACT87350.021.37436112-13
GTATAGG7150.020.597931
CATGGGG56100.020.5804944
AGTACTT90500.020.31542412-13
GTACTTT93350.020.0286514-15
CATGGGA63450.019.3197044
GTAATAC4200.019.231453
ATTCGCG4450.019.220980-81
TCGCGCT5000.018.994960-61
AGAGTAC163100.018.51212110-11
CGCGGAA4600.018.07078782-83
ATGGGTA10400.017.8177385
TATGTCG1600.001189189717.8177365
AATTCGC4950.017.28169478-79
ACTTTTT111050.017.17932116-17
GTGTAGG12000.017.0237461
Produced by FastQC (version 0.11.5)