FastQCFastQC Report
Thu 26 May 2016
SRR936747_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936747_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2445784
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT273501.1182508349061078No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT149440.6110106207252971No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT105470.4312318667552No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC92460.37803828956277413No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80990.33114126186122733No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT74200.30337920274235175No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71180.29103142387062797No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC67400.27557625693847043No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC66250.27087428816281406No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT65480.2677260134173745No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG55920.22863834255191792No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA51520.2106482011494065No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC50580.20680485275886995No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA44590.182313728440451No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43440.1776117596647946No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT31520.12887483113799092No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC31130.12728025042276833No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA30610.12515414280247153No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC27510.11247927045070209No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGCG3500.026.46247194-95
GTAAGGT10350.024.3237464
GTACATG127350.023.3534071
GGTAAGG9900.022.5514953
TACATGG131200.022.267972
ACATGGG128350.021.7617663
GAGTACT96550.021.72067612-13
AGGTAAG10700.020.8666822
TAAGGTG13300.020.3572045
GTACTTT102950.020.07048214-15
AGTACTT100000.019.26130912-13
CATGGGA63000.018.7738674
AGAGTAC176900.018.39284110-11
ACTTTTT118750.017.6391516-17
GAGTACA110000.017.5350991
AGTACAT103450.017.4498792
CATGGGG58900.017.3387624
GCACCGT7150.016.6074226
TACGAGA2304.2739826E-516.5217884
TACTTTT117250.016.36678714-15
Produced by FastQC (version 0.11.5)