##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936747_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2445784 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.850333062936055 31.0 31.0 34.0 30.0 34.0 2 31.95723048314978 31.0 31.0 34.0 30.0 34.0 3 32.08407733471149 33.0 31.0 34.0 30.0 34.0 4 35.81789111385143 37.0 35.0 37.0 35.0 37.0 5 35.674803253271754 37.0 35.0 37.0 33.0 37.0 6 35.73038256853426 37.0 35.0 37.0 33.0 37.0 7 35.65703389996827 37.0 35.0 37.0 33.0 37.0 8 35.62586475338787 37.0 35.0 37.0 33.0 37.0 9 37.21042782191723 39.0 37.0 39.0 33.0 39.0 10-11 37.10373585729566 39.0 37.0 39.0 33.0 39.0 12-13 36.98574199520481 39.0 37.0 39.0 33.0 39.0 14-15 38.033987056910995 40.0 37.0 41.0 33.0 41.0 16-17 38.03609599212359 40.0 37.0 41.0 33.0 41.0 18-19 38.19027641034531 40.0 37.5 41.0 33.0 41.0 20-21 38.20014522950514 40.0 37.5 41.0 33.0 41.0 22-23 38.09533466569411 40.0 37.0 41.0 33.0 41.0 24-25 37.95882567716528 40.0 37.0 41.0 33.0 41.0 26-27 37.743957356823 40.0 37.0 41.0 32.5 41.0 28-29 37.78499164276158 40.0 37.0 41.0 33.0 41.0 30-31 37.68149006617101 40.0 37.0 41.0 32.5 41.0 32-33 37.49251262580833 39.5 36.0 41.0 32.0 41.0 34-35 37.29012312616322 39.0 36.0 41.0 31.5 41.0 36-37 37.09690839419998 39.0 36.0 41.0 31.0 41.0 38-39 36.90176830823981 39.0 35.5 41.0 31.0 41.0 40-41 36.710127918082705 39.0 35.0 40.5 30.5 41.0 42-43 36.440772365834434 39.0 35.0 40.0 30.0 41.0 44-45 36.13959204901168 39.0 35.0 40.0 30.0 41.0 46-47 35.767650577483536 38.0 35.0 40.0 28.5 41.0 48-49 35.3589074096486 38.0 34.5 40.0 27.0 41.0 50-51 35.045479077465544 38.0 34.0 40.0 26.5 41.0 52-53 34.80125861482453 38.0 34.0 40.0 26.0 41.0 54-55 34.46877770072909 38.0 33.5 40.0 25.5 41.0 56-57 34.09389770314958 37.5 33.0 40.0 24.5 41.0 58-59 33.72010794902575 37.0 33.0 40.0 23.0 41.0 60-61 33.28577789371424 36.0 32.0 39.0 22.5 40.0 62-63 32.98891582412838 36.0 32.0 39.0 22.0 40.0 64-65 32.68160965972466 35.5 31.5 39.0 22.0 40.0 66-67 32.1959122310065 35.0 31.0 38.0 20.0 40.0 68-69 31.72253375604714 35.0 31.0 38.0 18.5 40.0 70-71 31.186163823134013 34.5 30.0 37.0 17.5 39.0 72-73 30.612876893462385 34.0 29.5 36.5 14.0 39.0 74-75 30.0827493351825 34.0 29.0 36.0 10.0 38.5 76-77 28.69610296739205 32.0 27.0 34.5 8.0 36.5 78-79 29.495502669082796 33.5 29.0 35.0 7.5 37.0 80-81 29.313299130258436 34.0 29.0 35.0 7.0 37.0 82-83 28.89792884408435 33.5 29.0 35.0 2.0 36.0 84-85 28.444169436058132 33.0 27.5 35.0 2.0 36.0 86-87 28.06020543923748 33.0 27.0 35.0 2.0 35.0 88-89 27.5519330406937 33.0 26.0 35.0 2.0 35.0 90-91 26.946792112467822 32.0 25.5 34.0 2.0 35.0 92-93 26.354372462981196 32.0 25.0 34.0 2.0 35.0 94-95 25.5807563137219 31.5 23.5 34.0 2.0 35.0 96-97 24.711213664003033 31.0 19.0 34.0 2.0 35.0 98-99 23.585390819467293 31.0 2.0 34.0 2.0 35.0 100-101 19.822403368408658 26.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2949634398510348 1101 2 0.37232307183856506 1101 3 0.3365934678800997 1101 4 0.17320192918901967 1101 5 0.11206803987612801 1101 6 0.20535280736913109 1101 7 0.2814750425720689 1101 8 0.18466275594509085 1101 9 0.35251211936694204 1101 10-11 0.30889424493912543 1101 12-13 0.3394715828185113 1101 14-15 0.3990931902949697 1101 16-17 0.3658737056193999 1101 18-19 0.3474406245190522 1101 20-21 0.2868120609912026 1101 22-23 0.3457691236309799 1101 24-25 0.251229319603965 1101 26-27 0.09958512156763533 1101 28-29 0.2464409058781527 1101 30-31 0.36531738323682106 1101 32-33 0.3350571625300347 1101 34-35 0.3991045605389374 1101 36-37 0.39906433635731986 1101 38-39 0.388848675929097 1101 40-41 0.43566394350506954 1101 42-43 0.4185456736464275 1101 44-45 0.6193728797696352 1101 46-47 0.6609081485977271 1101 48-49 0.6747896625340957 1101 50-51 0.6887915684050796 1101 52-53 0.62157277840803 1101 54-55 0.7985808903745735 1101 56-57 0.7329868122945911 1101 58-59 0.7010211297149382 1101 60-61 0.7390713090024335 1101 62-63 0.6160297830424213 1101 64-65 0.6664330067346924 1101 66-67 0.6445758876442866 1101 68-69 0.6555451948962272 1101 70-71 0.3803914887070263 1101 72-73 0.578297184549168 1101 74-75 0.694509690388756 1101 76-77 0.6422643707142157 1101 78-79 0.6454468614359818 1101 80-81 0.7694341040414976 1101 82-83 0.6995717865077218 1101 84-85 0.5790740419137848 1101 86-87 0.5841890662736695 1101 88-89 0.35713256694301165 1101 90-91 0.6444291285734813 1101 92-93 0.7262738976796932 1101 94-95 0.6855565619279318 1101 96-97 0.5920688347697727 1101 98-99 0.2641098044229082 1101 100-101 0.38902123258144883 1102 1 0.3199844163767587 1102 2 0.44677110216488103 1102 3 0.3787954113220877 1102 4 0.30742078103557446 1102 5 0.19150857164650859 1102 6 0.1378361277190976 1102 7 0.24400098722230723 1102 8 0.17541453696252773 1102 9 0.2430577563379117 1102 10-11 0.34924778109558474 1102 12-13 0.3851353154212447 1102 14-15 0.46646095869293447 1102 16-17 0.5292912630155868 1102 18-19 0.4112091743919635 1102 20-21 0.43116721167772454 1102 22-23 0.3683936535359962 1102 24-25 0.3974528383962834 1102 26-27 0.4589095626205051 1102 28-29 0.6312284644578625 1102 30-31 0.6652475203658881 1102 32-33 0.4992448896285211 1102 34-35 0.5049226980354291 1102 36-37 0.5446130734379935 1102 38-39 0.5950202789242809 1102 40-41 0.4758192846142961 1102 42-43 0.5750329402329015 1102 44-45 0.7485707020971404 1102 46-47 0.5745573751601043 1102 48-49 0.5405950109740587 1102 50-51 0.6775947847468018 1102 52-53 0.6668408274939779 1102 54-55 0.5923931244143077 1102 56-57 0.6300191859187549 1102 58-59 0.4578346585583333 1102 60-61 0.5623131228580647 1102 62-63 0.49032554587255106 1102 64-65 0.47278540530103896 1102 66-67 0.5797475595307944 1102 68-69 0.5827496731857451 1102 70-71 0.21687651190927326 1102 72-73 0.15971207108162133 1102 74-75 0.22222829228344665 1102 76-77 0.4799983583385057 1102 78-79 0.4851224362811095 1102 80-81 0.5162711089292493 1102 82-83 0.5475054883213311 1102 84-85 0.39029343605873024 1102 86-87 0.6201840778350487 1102 88-89 0.3977795094929455 1102 90-91 0.4629210621057176 1102 92-93 0.5814796972695255 1102 94-95 0.6332647771303002 1102 96-97 0.48649013879426306 1102 98-99 0.41214728483745944 1102 100-101 0.13571886501619446 1103 1 0.2125421779773653 1103 2 0.3093610997005598 1103 3 0.23374669110202717 1103 4 0.1732403663642259 1103 5 0.22343813213218056 1103 6 0.1788859935137097 1103 7 0.16817027916398786 1103 8 0.2121328654893304 1103 9 0.1570061255592563 1103 10-11 0.29484298033897716 1103 12-13 0.20560446816229927 1103 14-15 0.3086197672953972 1103 16-17 0.29819873788954254 1103 18-19 0.33429400019136324 1103 20-21 0.35687636894779473 1103 22-23 0.27030200091149226 1103 24-25 0.30665922830456793 1103 26-27 0.25342812159428263 1103 28-29 0.17746172208558875 1103 30-31 0.41163230020911357 1103 32-33 0.32191028195450144 1103 34-35 0.299359834631872 1103 36-37 0.3025486748313284 1103 38-39 0.0670972322288037 1103 40-41 0.22173501162121312 1103 42-43 0.5008431678159369 1103 44-45 0.3951336454245009 1103 46-47 0.2496572799175638 1103 48-49 0.35184145602977424 1103 50-51 0.36939410273032536 1103 52-53 0.27585157058556575 1103 54-55 0.39878686238410666 1103 56-57 0.2692817556023215 1103 58-59 0.14091828550063923 1103 60-61 0.019719062528913867 1103 62-63 0.14783022474535557 1103 64-65 0.2798005508622765 1103 66-67 0.09745601080724242 1103 68-69 0.20654432228693764 1103 70-71 0.12289427497514893 1103 72-73 0.32791571693923416 1103 74-75 0.4230585008179766 1103 76-77 0.36684706257380384 1103 78-79 0.27815607754310534 1103 80-81 0.4563728785755181 1103 82-83 0.27856154821090584 1103 84-85 0.4042887605160814 1103 86-87 0.364291695239789 1103 88-89 0.19902765292953362 1103 90-91 0.2291485918606817 1103 92-93 0.22316279271852935 1103 94-95 0.11371474815792482 1103 96-97 0.3064033215600226 1103 98-99 0.20408596995764228 1103 100-101 0.03842328680075724 1104 1 0.15517328084660775 1104 2 0.22035518355227168 1104 3 0.15228375071740885 1104 4 0.1473835220015971 1104 5 0.06715780110759084 1104 6 0.08761644001876334 1104 7 0.05108321653776926 1104 8 0.09872469917155513 1104 9 0.1233871288763666 1104 10-11 0.16135830618685532 1104 12-13 0.16865605979849363 1104 14-15 0.17449391268488057 1104 16-17 0.09358795271946718 1104 18-19 0.06915341639825812 1104 20-21 0.14700138052174339 1104 22-23 0.11385226939468396 1104 24-25 0.15853480659721697 1104 26-27 0.06842400677295757 1104 28-29 0.11532274019145206 1104 30-31 0.19907247990150978 1104 32-33 0.2414116212948514 1104 34-35 0.2676681833485759 1104 36-37 0.2218605413803303 1104 38-39 0.3540088953353333 1104 40-41 0.32777186889486387 1104 42-43 0.33525892146278125 1104 44-45 0.43656303269491303 1104 46-47 0.2495299665041557 1104 48-49 0.28196743560653204 1104 50-51 0.4230911450446797 1104 52-53 0.4910996221156907 1104 54-55 0.3045449089479675 1104 56-57 0.37329155347427445 1104 58-59 0.4978137191820906 1104 60-61 0.36680764028997004 1104 62-63 0.3163476167450625 1104 64-65 0.29495327515888903 1104 66-67 0.3999491198574603 1104 68-69 0.29912942074156135 1104 70-71 0.08456727197013336 1104 72-73 0.21046168736043924 1104 74-75 0.3216480656829077 1104 76-77 0.287891298036115 1104 78-79 0.20815959990785515 1104 80-81 0.26939795535206557 1104 82-83 0.20041247966270603 1104 84-85 0.10235508586733033 1104 86-87 0.33035348425143596 1104 88-89 0.2545078114618491 1104 90-91 0.27179753317640376 1104 92-93 0.3159350977371851 1104 94-95 0.2352724297944242 1104 96-97 0.6090190496956254 1104 98-99 0.4573404342635925 1104 100-101 0.2236906077380425 1105 1 0.07884859448698833 1105 2 0.23237990494504146 1105 3 0.16256789380561543 1105 4 0.13947211453477593 1105 5 0.008409460110918587 1105 6 0.10886432261945345 1105 7 0.11329960596914646 1105 8 0.10199784153647329 1105 9 0.13250854568276083 1105 10-11 0.11034636292340139 1105 12-13 0.04421017276131067 1105 14-15 0.07449726775334398 1105 16-17 0.07671094406729395 1105 18-19 0.10854980057075636 1105 20-21 0.022195486836842804 1105 22-23 -0.03234593088418336 1105 24-25 0.08050660373573493 1105 26-27 0.20773979127769593 1105 28-29 0.05285097543325179 1105 30-31 0.04156918140106569 1105 32-33 0.09262073334868148 1105 34-35 -0.10497240377280548 1105 36-37 0.11238075644801171 1105 38-39 -0.011109082135774884 1105 40-41 0.21636440207297625 1105 42-43 0.26255872335769226 1105 44-45 0.20752998683112622 1105 46-47 0.2785771348653938 1105 48-49 0.2873048033446679 1105 50-51 0.34421356369011846 1105 52-53 0.30823387869103414 1105 54-55 0.37817087187211484 1105 56-57 0.48647720657735505 1105 58-59 0.4655374924949385 1105 60-61 0.36321763901816695 1105 62-63 0.2749190754117876 1105 64-65 0.24003220899615485 1105 66-67 0.360247892604626 1105 68-69 0.4328881195367842 1105 70-71 0.22774996793148716 1105 72-73 0.3978807268277471 1105 74-75 0.40732785326805754 1105 76-77 0.28157744932785533 1105 78-79 0.30937101369469033 1105 80-81 0.28794578809487703 1105 82-83 0.3463947071628759 1105 84-85 0.5024855434128384 1105 86-87 0.44676015912111566 1105 88-89 0.37286237665668054 1105 90-91 0.5032868012487128 1105 92-93 0.567862194440405 1105 94-95 0.6057171636243623 1105 96-97 0.4477022867592986 1105 98-99 0.329913763966168 1105 100-101 0.38964111033510207 1106 1 0.041703173772276614 1106 2 0.19240005630084767 1106 3 0.043621942095320776 1106 4 0.0641347264152472 1106 5 0.1150969155242052 1106 6 0.0516410911951084 1106 7 0.10314302846364853 1106 8 0.059929312217725794 1106 9 0.23173926399769584 1106 10-11 0.07258132479026358 1106 12-13 0.11681021247565582 1106 14-15 0.16611386246839288 1106 16-17 0.1440822953113141 1106 18-19 -0.006884005167229645 1106 20-21 0.06556760294983377 1106 22-23 0.08799039083148585 1106 24-25 0.2271816300281131 1106 26-27 0.11305894021386109 1106 28-29 0.15987968008349895 1106 30-31 0.12368669175253899 1106 32-33 0.07044063612205775 1106 34-35 0.15698713171638445 1106 36-37 0.23779499919094604 1106 38-39 0.35489911078771996 1106 40-41 0.10140974262353808 1106 42-43 0.18085784596472365 1106 44-45 0.2602820442551348 1106 46-47 0.2070148164298402 1106 48-49 0.18654581797278524 1106 50-51 0.09779815158018579 1106 52-53 0.20746055202579328 1106 54-55 0.19367895815521763 1106 56-57 0.23313200796883393 1106 58-59 0.12397936612975968 1106 60-61 0.09654864342352454 1106 62-63 0.21729097824400867 1106 64-65 0.2605762956028599 1106 66-67 3.516647325056965E-4 1106 68-69 0.012334104342365038 1106 70-71 -0.048596091154639254 1106 72-73 -0.01755785934136256 1106 74-75 0.024088412504955414 1106 76-77 -0.18480766220328348 1106 78-79 -0.21580604611953547 1106 80-81 -0.18534998821036197 1106 82-83 0.08264030365036135 1106 84-85 0.1529466711533125 1106 86-87 0.1522510358521849 1106 88-89 0.15153209299325354 1106 90-91 0.25944142245470303 1106 92-93 -0.04741606342340532 1106 94-95 -0.08801458799914741 1106 96-97 0.11775325350956223 1106 98-99 0.23651186209003328 1106 100-101 0.08161366330595499 1107 1 0.12224041595198187 1107 2 0.12322100276409742 1107 3 0.07174193791667705 1107 4 0.0498899808475457 1107 5 0.04466870451404503 1107 6 0.11338244161319011 1107 7 -0.011856162997027297 1107 8 0.009327773274030449 1107 9 0.022596186883077962 1107 10-11 0.16608016779196788 1107 12-13 0.1310754219588972 1107 14-15 0.251735743688954 1107 16-17 0.20177629823922416 1107 18-19 0.2286305478250128 1107 20-21 0.15866890219381702 1107 22-23 0.12233048679723169 1107 24-25 0.2150600514731451 1107 26-27 0.27322077687175295 1107 28-29 0.2705689992075335 1107 30-31 0.2608956712614301 1107 32-33 0.08572052667588537 1107 34-35 0.12119459861278159 1107 36-37 0.17961565801454782 1107 38-39 0.180370961455516 1107 40-41 0.2418332741229463 1107 42-43 0.17339903496113607 1107 44-45 0.15912225541984526 1107 46-47 0.18172570995880477 1107 48-49 0.10533498383210826 1107 50-51 0.12535166901243144 1107 52-53 0.0635390504609532 1107 54-55 0.14266391932386568 1107 56-57 0.13695647050484183 1107 58-59 -0.0063769206569972425 1107 60-61 0.06372831517087008 1107 62-63 -0.22060182518362836 1107 64-65 -0.09942977201964709 1107 66-67 -0.011465817175533743 1107 68-69 -0.06375896211098109 1107 70-71 -0.19109241695162282 1107 72-73 -0.031199277161569228 1107 74-75 -0.09160762536616573 1107 76-77 -0.3576203486860763 1107 78-79 -0.24491768321846408 1107 80-81 0.00927487101077773 1107 82-83 0.1447844211332736 1107 84-85 0.2537992150469819 1107 86-87 0.33840698233689537 1107 88-89 0.30885867561952907 1107 90-91 0.06000439478835062 1107 92-93 0.13542301149312763 1107 94-95 0.28557293674001016 1107 96-97 0.307534604442818 1107 98-99 0.515673943456278 1107 100-101 0.31155323035819293 1108 1 -0.008574192060713415 1108 2 0.1671711946436787 1108 3 -0.011335519184960674 1108 4 0.0030788406843953453 1108 5 0.06315925732811678 1108 6 0.03659253126735962 1108 7 0.06113450956932098 1108 8 0.006791315049923696 1108 9 0.04316088236461013 1108 10-11 -0.047140222214181904 1108 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-1.4670835916049398 2316 50-51 -1.4430541018597367 2316 52-53 -1.8069045100913073 2316 54-55 -1.6157720549183736 2316 56-57 -1.6687434489066177 2316 58-59 -1.879905175270565 2316 60-61 -1.3867443980603227 2316 62-63 -1.4165576776665958 2316 64-65 -1.393091011063614 2316 66-67 -1.4493025916853028 2316 68-69 -1.6202089878804706 2316 70-71 -1.2417269295064948 2316 72-73 -0.9718990516807615 2316 74-75 -1.252159542219701 2316 76-77 -1.4247212891702006 2316 78-79 -1.4075194255879317 2316 80-81 -1.4951028309410326 2316 82-83 -1.6023958243088892 2316 84-85 -1.3802791074881746 2316 86-87 -1.6270171013173176 2316 88-89 -1.6823450306356698 2316 90-91 -2.0416074827504893 2316 92-93 -1.9696007677273286 2316 94-95 -2.1021000917340764 2316 96-97 -1.8910950880886865 2316 98-99 -1.045226141505701 2316 100-101 -0.835032139007744 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 23.0 4 43.0 5 77.0 6 208.0 7 466.0 8 1084.0 9 1961.0 10 3019.0 11 4867.0 12 7043.0 13 9671.0 14 12344.0 15 16511.0 16 20758.0 17 25969.0 18 23236.0 19 17218.0 20 16839.0 21 17578.0 22 19138.0 23 21320.0 24 24940.0 25 30163.0 26 36713.0 27 44929.0 28 55604.0 29 67711.0 30 82550.0 31 101382.0 32 125055.0 33 158085.0 34 205950.0 35 273808.0 36 371694.0 37 433120.0 38 206951.0 39 7751.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.82000556118944 19.26620923157447 14.878267198796166 22.035518008439922 2 15.7366433782609 21.371167247173048 38.16310825387661 24.729081120689443 3 20.99444893312878 24.28121534672088 29.70462730653861 25.019708413611724 4 13.0174938199624 16.730954322101187 37.278771826388166 32.97278003154824 5 13.379851205505275 38.37143832633794 33.55076656355864 14.697943904598155 6 31.630103067155563 36.40673092963238 16.591653228576195 15.371512774635862 7 28.723802249840848 33.143304795661734 20.179673256675905 17.953219697821506 8 26.83187910407484 32.7288673366752 19.174360316305087 21.264893242944876 9 25.98982575730318 17.71051736375739 20.982474331339155 35.31718254760028 10-11 25.83576472820167 25.95456099148576 26.333764551571193 21.875909728741377 12-13 27.553496138661473 23.600673649022156 26.78503089397919 22.06079931833719 14-15 23.343125062991373 25.31683765225002 26.617899505329913 24.7221377794287 16-17 22.08725607449886 28.62829280260051 26.936081041021392 22.348370081879235 18-19 21.902371550228928 27.77167419948629 28.843945927248253 21.482008323036524 20-21 23.255494940760027 25.648726527099313 28.533254830529003 22.56252370161166 22-23 22.445485426254656 25.71421328596989 28.98261405006525 22.857687237710202 24-25 23.394940948153643 26.002458525918886 28.758885988875914 21.843714537051557 26-27 22.727597962533046 25.589872686936122 29.906569759692402 21.775959590838433 28-29 21.61441174756954 27.43203862158634 29.119780683687125 21.833768947156994 30-31 22.101533197193604 25.860490237947413 29.02538115273647 23.012595412122515 32-33 22.196751287051146 26.19023865082776 29.165881276865953 22.447128785255146 34-35 23.55405463697094 25.785077571954584 28.62736207107684 22.03350571999764 36-37 23.032084096558943 25.335110558681144 29.543659230668585 22.089146114091328 38-39 22.39673789154859 26.415411806436513 29.186527549340003 22.00132275267489 40-41 22.45061756283825 26.554844286094827 28.850468571727628 22.1440695793393 42-43 22.149989962125936 26.696922032090942 27.851686424173984 23.301401581609145 44-45 22.750142496972195 25.87264573330945 27.99327463357629 23.38393713614206 46-47 23.61204014745374 26.83487176300115 27.6353717253854 21.917716364159716 48-49 23.429738710075085 26.646445041084842 27.498316965701264 22.42549928313881 50-51 22.038989091738724 26.964490806746856 28.486307533728766 22.51021256778565 52-53 23.133826730019663 27.531588250229888 26.828557258316106 22.506027761434346 54-55 22.32703991076879 26.5919761417723 27.533618095814795 23.547365851644116 56-57 23.32750836176815 26.586343804992463 27.07450463718033 23.011643196059055 58-59 24.284768321585982 26.14552269395397 26.881683719186327 22.68802526527372 60-61 23.062841862409673 26.708992610507615 28.418869425042498 21.809296102040214 62-63 22.476097098656236 26.921569617551533 28.09068516164881 22.51164812214342 64-65 22.112281054682867 27.80271267717817 27.367438878489693 22.717567389649272 66-67 22.792184826362412 26.94006554213321 26.582670790819197 23.685078840685186 68-69 22.460409716654148 26.804699929142075 27.35894958538094 23.37594076882284 70-71 23.468342174138996 26.70183634975653 26.991757258858218 22.838064217246256 72-73 23.166299119296944 26.107707087064263 28.17609890598522 22.549894887653576 74-75 22.305571388966808 28.23268054245948 26.8268099224649 22.63493814610881 76-77 22.680802486273123 26.89086247198711 26.89713866794998 23.531196373789786 78-79 22.992628054283102 26.966850573453062 27.163651143287414 22.876870228976422 80-81 23.48444099895061 27.043126393130162 26.75372126622716 22.718711341692064 82-83 23.166513268749473 26.779535095957858 27.468067541968406 22.585884093324267 84-85 22.661853663243633 27.461493689730553 26.9952331313014 22.88141951572441 86-87 22.615018551428722 27.4980236273384 26.750610288749733 23.136347532483143 88-89 22.367284806229314 27.532438220798237 26.08781605134835 24.01246092162409 90-91 22.86941506283131 26.670369922096697 26.64618358878572 23.814031426286274 92-93 23.457790863673917 27.05141491024833 26.954429175369793 22.53636505070796 94-95 22.8412606482162 26.646518984689298 27.17138361340492 23.34083675368958 96-97 21.92784478426745 26.78475563858282 28.025953242785643 23.261446334364084 98-99 22.372674911387715 27.395761738546643 26.39002407015877 23.841539279906875 100-101 21.90128237602339 26.6974917749624 26.866929696142282 24.534296152871928 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 382.0 1 276.5 2 295.5 3 399.0 4 328.0 5 278.0 6 452.5 7 894.0 8 1385.0 9 1706.0 10 1711.5 11 1606.5 12 1704.0 13 1950.0 14 2154.5 15 2345.0 16 2639.5 17 2877.5 18 3092.5 19 3451.5 20 3832.0 21 4361.0 22 5199.5 23 6170.0 24 7422.5 25 9255.5 26 12099.0 27 15316.5 28 17576.5 29 20573.0 30 24812.5 31 29780.0 32 36454.0 33 41529.5 34 46203.0 35 50986.5 36 56890.5 37 62845.5 38 64263.0 39 64325.0 40 64678.5 41 67048.5 42 69816.5 43 72712.5 44 75976.0 45 79284.5 46 81706.0 47 100117.0 48 142107.5 49 140358.5 50 101369.0 51 83194.0 52 77569.5 53 77074.5 54 76269.0 55 71708.0 56 65276.0 57 59520.0 58 55380.0 59 50257.0 60 43461.0 61 37086.5 62 31988.0 63 25746.5 64 19453.0 65 15388.0 66 11597.0 67 9138.5 68 7018.0 69 4686.0 70 3633.5 71 3140.0 72 2729.5 73 2529.5 74 2085.0 75 1672.0 76 1231.0 77 726.5 78 470.0 79 336.5 80 219.0 81 153.0 82 100.0 83 54.0 84 32.0 85 23.5 86 15.0 87 6.5 88 4.5 89 4.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010794084841506854 2 0.008913297331244296 3 0.005233495680730596 4 2.453201100342467E-4 5 0.005274382365736303 6 0.0 7 4.497535350627856E-4 8 0.0017990141402511423 9 0.0 10-11 0.0 12-13 0.0 14-15 5.110835625713473E-4 16-17 0.004456648665622148 18-19 0.001717240770239727 20-21 4.7019687756563944E-4 22-23 6.541869600913245E-4 24-25 3.8842350755422396E-4 26-27 8.177337001141557E-5 28-29 6.133002750856167E-5 30-31 5.110835625713473E-4 32-33 0.003148274745439499 34-35 9.19950412628425E-4 36-37 9.812804401369867E-4 38-39 0.005172165653222034 40-41 2.0443342502853892E-5 42-43 0.0019216741952682655 44-45 0.004824628830673518 46-47 0.0 48-49 0.0021465509627996582 50-51 9.403937551312789E-4 52-53 6.950736450970323E-4 54-55 1.2266005501712334E-4 56-57 0.011386941774089616 58-59 0.0022692110178167816 60-61 0.003332264827965184 62-63 1.4310339751997722E-4 64-65 0.003352708170468038 66-67 0.0011857138651655257 68-69 0.00183990082525685 70-71 0.004783742145667811 72-73 0.002759851237885275 74-75 0.007441376671038816 76-77 0.0014310339751997722 78-79 0.006582756285918953 80-81 0.003168718087942353 82-83 0.0030665013754280833 84-85 0.0019830042227768274 86-87 6.337436175884706E-4 88-89 0.0023714277303310514 90-91 0.007686696781073062 92-93 0.002596304497862444 94-95 4.293101925599317E-4 96-97 4.0886685005707784E-5 98-99 6.950736450970323E-4 100-101 0.002064777592788243 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2445784.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.55585509800586 #Duplication Level Percentage of deduplicated Percentage of total 1 74.01023765387073 33.71599662328712 2 14.356592980153312 13.080537390098247 3 4.902573816326082 6.700228271514856 4 2.1693242776939923 3.953016898208549 5 1.1635780462124181 2.6503896434236838 6 0.7085177853561602 1.9366280138427143 7 0.45961046168637765 1.4656563315878532 8 0.33644605065720257 1.2261670025628686 9 0.23956503169872992 0.9822230883554868 >10 1.3910235078126598 12.0932485858994 >50 0.15315442717156577 4.869220023070518 >100 0.10013142351806797 8.381737158269829 >500 0.005205662086115509 1.6450329451208345 >1k 0.002872089426822311 2.375449979188332 >5k 8.975279458819721E-4 2.763923570806975 >10k+ 2.6925838376459164E-4 2.1605444747627356 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 27350 1.1182508349061078 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 14944 0.6110106207252971 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10547 0.4312318667552 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 9246 0.37803828956277413 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8099 0.33114126186122733 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 7420 0.30337920274235175 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7118 0.29103142387062797 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6740 0.27557625693847043 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6625 0.27087428816281406 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6548 0.2677260134173745 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5592 0.22863834255191792 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5152 0.2106482011494065 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5058 0.20680485275886995 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4459 0.182313728440451 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4344 0.1776117596647946 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3152 0.12887483113799092 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3113 0.12728025042276833 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3061 0.12515414280247153 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2751 0.11247927045070209 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 4.0886685005707784E-5 0.0 16-17 0.0 0.0 0.0 4.0886685005707784E-5 0.0 18-19 0.0 0.0 0.0 4.0886685005707784E-5 0.0 20-21 0.0 0.0 0.0 8.177337001141557E-5 0.0 22-23 0.0 0.0 0.0 1.0221671251426945E-4 0.0 24-25 0.0 0.0 0.0 1.4310339751997722E-4 0.0 26-27 0.0 0.0 0.0 2.044334250285389E-4 0.0 28-29 0.0 4.0886685005707784E-5 0.0 6.133002750856167E-4 0.0 30-31 0.0 4.0886685005707784E-5 0.0 0.0011652705226626718 0.0 32-33 0.0 4.0886685005707784E-5 0.0 0.0016150240577254573 0.0 34-35 0.0 4.0886685005707784E-5 0.0 0.00220788099030822 0.0 36-37 0.0 4.0886685005707784E-5 0.0 0.003291378142959476 0.0 38-39 0.0 4.0886685005707784E-5 0.0 0.004109111843073632 0.0 40-41 0.0 4.0886685005707784E-5 0.0 0.0060921160658504595 0.0 42-43 0.0 4.0886685005707784E-5 0.0 0.008606647193701487 0.0 44-45 0.0 4.0886685005707784E-5 0.0 0.013758369504420668 0.0 46-47 0.0 4.0886685005707784E-5 0.0 0.026882995391252867 0.0 48-49 0.0 4.0886685005707784E-5 0.0 0.03624604625755995 0.0 50-51 0.0 4.0886685005707784E-5 0.0 0.04838939170425516 0.0 52-53 0.0 4.0886685005707784E-5 0.0 0.06588889288669808 0.0 54-55 0.0 4.0886685005707784E-5 0.0 0.08784504273476317 0.0 56-57 0.0 4.0886685005707784E-5 0.0 0.11043493620041672 0.0 58-59 0.0 4.0886685005707784E-5 0.0 0.14578147538785108 0.0 60-61 0.0 4.0886685005707784E-5 0.0 0.1907363855516268 0.0 62-63 0.0 4.0886685005707784E-5 0.0 0.24568809019929805 0.0 64-65 0.0 4.0886685005707784E-5 0.0 0.2906634437055766 0.0 66-67 0.0 4.0886685005707784E-5 0.0 0.3577380504574402 0.0 68-69 0.0 4.0886685005707784E-5 0.0 0.4440498425044893 0.0 70-71 0.0 4.0886685005707784E-5 0.0 0.5061362736856566 0.0 72-73 0.0 4.0886685005707784E-5 0.0 0.6007276194463615 0.0 74-75 0.0 4.0886685005707784E-5 0.0 0.6794344880823491 0.0 76-77 0.0 4.0886685005707784E-5 0.0 0.748287665631961 0.0 78-79 0.0 4.0886685005707784E-5 0.0 0.8440442819153287 0.0 80-81 0.0 4.0886685005707784E-5 0.0 0.9426425228065929 0.0 82-83 0.0 4.0886685005707784E-5 0.0 1.065486567906242 0.0 84-85 0.0 4.0886685005707784E-5 0.0 1.2352276407074378 0.0 86-87 0.0 4.0886685005707784E-5 0.0 1.4669733713197894 0.0 88-89 0.0 4.0886685005707784E-5 0.0 1.7566146479002236 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 350 0.0 26.462471 94-95 GTAAGGT 1035 0.0 24.323746 4 GTACATG 12735 0.0 23.353407 1 GGTAAGG 990 0.0 22.551495 3 TACATGG 13120 0.0 22.26797 2 ACATGGG 12835 0.0 21.761766 3 GAGTACT 9655 0.0 21.720676 12-13 AGGTAAG 1070 0.0 20.866682 2 TAAGGTG 1330 0.0 20.357204 5 GTACTTT 10295 0.0 20.070482 14-15 AGTACTT 10000 0.0 19.261309 12-13 CATGGGA 6300 0.0 18.773867 4 AGAGTAC 17690 0.0 18.392841 10-11 ACTTTTT 11875 0.0 17.63915 16-17 GAGTACA 11000 0.0 17.535099 1 AGTACAT 10345 0.0 17.449879 2 CATGGGG 5890 0.0 17.338762 4 GCACCGT 715 0.0 16.607422 6 TACGAGA 230 4.2739826E-5 16.521788 4 TACTTTT 11725 0.0 16.366787 14-15 >>END_MODULE