FastQCFastQC Report
Thu 26 May 2016
SRR936746_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936746_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2527203
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT261541.0348990563876348No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT150230.5944516526768923No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT102110.4040435216324134No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC94640.37448515216229167No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84220.3332537987648796No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT80270.317623871133423No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66580.2634533118233874No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC62720.24817950912530573No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT61850.24473696810268109No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC59700.23622953913872372No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG52630.2082539471502685No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC49370.1953543106746866No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA48990.19385067206710344No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA40800.16144330312998204No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39900.15788205379623244No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC29110.11518663122827885No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT28390.11233763176127917No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA27680.10952820173132116No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC26310.10410718885661342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG128350.026.2148971
TACATGG132100.024.6053562
ACATGGG132750.023.733153
GAGTACT89250.023.15917212-13
GTACTTT94700.021.7487314-15
ATGGGCG4600.021.695635
AGTACTT92550.020.53645912-13
CATGGGG61550.020.3054544
GCGCGAC1200.004754551719.7992139
CATGGGA66400.019.5379754
ATTCGCG5000.019.4764580-81
ACTTTTT110000.019.45736116-17
AGAGTAC170150.019.19975510-11
GTATAGG8600.018.7884641
GATGTGC12200.017.9166668
CGCGGAA5350.017.7558782-83
GTACCTG18700.017.2813681
ATACCGT2202.9069177E-517.2813666
GTAATAC3301.2058081E-817.2799993
TACTTTT109550.017.1308714-15
Produced by FastQC (version 0.11.5)