##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936746_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2527203 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.21702174300996 31.0 31.0 34.0 28.0 34.0 2 31.23864762743634 31.0 31.0 34.0 28.0 34.0 3 31.28811852470894 31.0 31.0 34.0 28.0 34.0 4 34.92156664897913 37.0 35.0 37.0 32.0 37.0 5 34.84922105584712 37.0 35.0 37.0 32.0 37.0 6 34.98304607900513 37.0 35.0 37.0 32.0 37.0 7 34.81173811522066 37.0 35.0 37.0 32.0 37.0 8 34.79263003407324 37.0 35.0 37.0 32.0 37.0 9 36.25023118443592 39.0 35.0 39.0 32.0 39.0 10-11 36.108149602544785 39.0 35.0 39.0 31.0 39.0 12-13 36.01894663784429 39.0 35.0 39.0 30.0 39.0 14-15 37.02355904927305 39.0 36.0 41.0 31.0 41.0 16-17 36.97974025038748 39.0 36.0 41.0 31.0 41.0 18-19 36.95167345084665 39.0 36.0 41.0 31.0 41.0 20-21 36.84816514541966 39.0 36.0 41.0 30.0 41.0 22-23 36.73839695505268 39.0 36.0 41.0 30.0 41.0 24-25 36.566396723967166 39.0 36.0 41.0 30.0 41.0 26-27 36.35299736507119 39.0 35.5 40.0 29.5 41.0 28-29 36.48694940612211 39.0 36.0 41.0 30.0 41.0 30-31 36.43255607088152 39.0 35.5 40.5 30.0 41.0 32-33 36.26750086953838 39.0 35.0 40.0 30.0 41.0 34-35 36.073462242645334 38.5 35.0 40.0 29.0 41.0 36-37 35.81733560778457 38.0 35.0 40.0 28.0 41.0 38-39 35.62493080294698 38.0 35.0 40.0 27.0 41.0 40-41 35.49502117558423 38.0 34.5 40.0 27.0 41.0 42-43 35.21466360240946 38.0 34.0 40.0 26.0 41.0 44-45 34.91547434060501 38.0 34.0 40.0 25.5 41.0 46-47 34.54322565302431 38.0 33.0 40.0 24.0 41.0 48-49 34.24934146564404 38.0 33.0 40.0 23.0 41.0 50-51 33.51762917343798 37.5 32.0 39.5 22.5 40.5 52-53 33.71788218041843 37.5 33.0 39.5 23.0 40.5 54-55 34.075433591998745 38.0 33.0 40.0 23.0 41.0 56-57 33.96047428718627 38.0 33.0 40.0 22.5 41.0 58-59 33.713064007917055 37.5 33.0 40.0 22.0 41.0 60-61 33.33669693332906 37.0 32.5 40.0 20.5 41.0 62-63 33.03950315823462 36.5 32.0 39.5 20.0 41.0 64-65 32.747401178298695 36.0 32.0 39.0 19.0 41.0 66-67 32.34209855717962 35.5 31.0 39.0 18.0 41.0 68-69 31.94449654420322 35.0 31.0 38.5 16.0 40.0 70-71 31.508346183508014 35.0 31.0 37.5 13.0 40.0 72-73 31.09336171253358 35.0 30.5 37.0 10.0 39.5 74-75 30.683873238517048 35.0 30.0 37.0 7.5 39.0 76-77 30.28754852696835 34.0 30.0 36.0 7.0 39.0 78-79 29.82463874093217 34.0 29.0 36.0 3.5 37.5 80-81 29.37963570793482 34.0 29.0 35.0 2.0 37.0 82-83 28.96823385379014 34.0 29.0 35.0 2.0 37.0 84-85 28.57870539090053 34.0 28.0 35.0 2.0 36.0 86-87 28.290981571326085 34.0 27.5 35.0 2.0 36.0 88-89 27.90030163781857 34.0 27.0 35.0 2.0 35.5 90-91 27.524327289893215 33.0 26.0 35.0 2.0 35.0 92-93 27.142963584642786 33.0 25.5 35.0 2.0 35.0 94-95 26.695235008822003 33.0 25.0 35.0 2.0 35.0 96-97 26.09718669216521 33.0 24.5 35.0 2.0 35.0 98-99 25.44910539438264 32.5 21.5 35.0 2.0 35.0 100-101 23.368586931876862 30.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.30325989438733814 1101 2 0.3746653803336777 1101 3 0.3437728395925923 1101 4 0.31483484434253484 1101 5 0.3710949292511714 1101 6 0.140167926404132 1101 7 0.21801102490952218 1101 8 0.36264038561520806 1101 9 0.4780119565032166 1101 10-11 0.49118332793459274 1101 12-13 0.4429150082587512 1101 14-15 0.5804063903143089 1101 16-17 0.6719389956815363 1101 18-19 0.6344945399257895 1101 20-21 0.7147464049465952 1101 22-23 0.6135740110668948 1101 24-25 0.5317806470552213 1101 26-27 0.5319121795288311 1101 28-29 0.6131429691559589 1101 30-31 0.4580065364485648 1101 32-33 0.4686449020362815 1101 34-35 0.5053446805360267 1101 36-37 0.535939373747901 1101 38-39 0.4684835119275945 1101 40-41 0.4586437565181356 1101 42-43 0.2061815803744409 1101 44-45 0.3844705207348369 1101 46-47 0.5286998647879386 1101 48-49 0.5535804674161611 1101 50-51 0.7696593069405466 1101 52-53 0.674723176784795 1101 54-55 0.8321903581601262 1101 56-57 0.7792428384395365 1101 58-59 0.6723306774141946 1101 60-61 0.6513585183690012 1101 62-63 0.6824123584649584 1101 64-65 0.6537397777463596 1101 66-67 0.6080012467062019 1101 68-69 0.5996842758353758 1101 70-71 0.5076644690421013 1101 72-73 0.5780577364520667 1101 74-75 0.4162143940921723 1101 76-77 0.5237275878386143 1101 78-79 0.539882207709649 1101 80-81 0.458471536246865 1101 82-83 0.5115961951338939 1101 84-85 0.5229590831090078 1101 86-87 0.4216666925111774 1101 88-89 0.4227223483599474 1101 90-91 0.36957944840550994 1101 92-93 0.3404182839698606 1101 94-95 0.3678771153712326 1101 96-97 0.31124038980010127 1101 98-99 0.1333893017239518 1101 100-101 0.5086955558236745 1102 1 0.24991329448582533 1102 2 0.2838238381424105 1102 3 0.2827999894487476 1102 4 0.3164208882370403 1102 5 0.4494267866450201 1102 6 0.40211780120258567 1102 7 0.14196434063966024 1102 8 0.31366486324408527 1102 9 0.4076315964280184 1102 10-11 0.4300073250218901 1102 12-13 0.6230272472082845 1102 14-15 0.6237194725420423 1102 16-17 0.409574709499239 1102 18-19 0.5374758363613168 1102 20-21 0.36011377878078576 1102 22-23 0.16764084917220856 1102 24-25 0.3306541708624309 1102 26-27 0.35349119160181175 1102 28-29 0.4309583355435791 1102 30-31 0.46502062643561715 1102 32-33 0.464264874300774 1102 34-35 0.3990900407834772 1102 36-37 0.20202478369770205 1102 38-39 0.1696790566843589 1102 40-41 0.08852372920143381 1102 42-43 0.29156755037065096 1102 44-45 0.2509643640069541 1102 46-47 0.3511294840901229 1102 48-49 0.44585698079452385 1102 50-51 0.47058026615936654 1102 52-53 0.261401691496566 1102 54-55 0.1684623984623954 1102 56-57 0.40407286897831085 1102 58-59 0.41916635772772537 1102 60-61 0.19818666700049903 1102 62-63 0.3404165775578747 1102 64-65 0.4913423589179686 1102 66-67 0.45451688119468514 1102 68-69 0.42126886067758207 1102 70-71 0.4660041350677311 1102 72-73 0.3141768126828204 1102 74-75 0.14775366725445593 1102 76-77 0.04598894328469427 1102 78-79 0.04610563995457184 1102 80-81 0.22000041366147727 1102 82-83 0.3309898373134743 1102 84-85 0.22410447294279123 1102 86-87 0.12035449111872865 1102 88-89 0.05190808909580724 1102 90-91 -0.15175851606791824 1102 92-93 -0.05524849955608957 1102 94-95 -0.05071570298418138 1102 96-97 -0.2008060796648401 1102 98-99 -0.11527357140671768 1102 100-101 0.11745203121263614 1103 1 0.20726574149331256 1103 2 0.41182760888171543 1103 3 0.5057529289244869 1103 4 0.39053764776273425 1103 5 0.4973302702707372 1103 6 0.24008945874822984 1103 7 0.2863400002238521 1103 8 0.09791146372283066 1103 9 0.5140316413603259 1103 10-11 0.47122744767303715 1103 12-13 0.4511897489674368 1103 14-15 0.6535401773345697 1103 16-17 0.6961890317346899 1103 18-19 0.5688013584520206 1103 20-21 0.3450471765341092 1103 22-23 0.37673466226851104 1103 24-25 0.3968999588579081 1103 26-27 0.43068332474695126 1103 28-29 0.5132832184339762 1103 30-31 0.328739482972324 1103 32-33 0.3937012132371436 1103 34-35 0.29872179056160775 1103 36-37 0.48036572222756746 1103 38-39 0.507368132167116 1103 40-41 0.4260580307705837 1103 42-43 0.5166599599707169 1103 44-45 0.5108890417660703 1103 46-47 0.5070389116723675 1103 48-49 0.6293448859311965 1103 50-51 0.03260838522869136 1103 52-53 0.40790333832291736 1103 54-55 0.6197714028257479 1103 56-57 0.6024033530887749 1103 58-59 0.528887739153518 1103 60-61 0.6495950777021733 1103 62-63 0.3790909884516509 1103 64-65 0.36043210959793726 1103 66-67 0.45525875883521394 1103 68-69 0.3473761244247733 1103 70-71 0.3090295597944781 1103 72-73 0.11637583087370018 1103 74-75 0.19782510259582153 1103 76-77 0.27634597957806406 1103 78-79 0.3636320624145952 1103 80-81 0.5150381066940675 1103 82-83 0.38030958202906007 1103 84-85 0.16987153405423072 1103 86-87 0.299368472787755 1103 88-89 0.3824382328313085 1103 90-91 0.5548179870819681 1103 92-93 0.5496006150254367 1103 94-95 0.60541288126851 1103 96-97 0.5810844469909 1103 98-99 0.625223469858561 1103 100-101 0.5404451480816199 1104 1 0.2376129622435883 1104 2 0.1818878055200308 1104 3 0.3391644855889666 1104 4 0.29763876176390625 1104 5 0.30851585335754095 1104 6 0.23548265967721704 1104 7 0.3975163479109156 1104 8 0.4464291063643415 1104 9 0.4510822483229049 1104 10-11 0.5662094462135414 1104 12-13 0.5140565533071708 1104 14-15 0.4714441352332628 1104 16-17 0.4265685006840698 1104 18-19 0.5519054974251034 1104 20-21 0.5226523919611665 1104 22-23 0.5958722273326558 1104 24-25 0.5392120184731652 1104 26-27 0.505051532564643 1104 28-29 0.4176540606115893 1104 30-31 0.34694889036246224 1104 32-33 0.3670795678825485 1104 34-35 0.43718382398466815 1104 36-37 0.47241699535012316 1104 38-39 0.36971283362146323 1104 40-41 0.13635156508236435 1104 42-43 0.2142487636382313 1104 44-45 0.058845444623287335 1104 46-47 0.25112825583786247 1104 48-49 0.17253400572190714 1104 50-51 0.2102145847557111 1104 52-53 0.3626155117909846 1104 54-55 0.4778135435005595 1104 56-57 0.5682693156445282 1104 58-59 0.41297136039917604 1104 60-61 0.4580185192663109 1104 62-63 0.24489048640927535 1104 64-65 0.38842725571407755 1104 66-67 0.42711902572312255 1104 68-69 0.38617615833837604 1104 70-71 0.42720687038551475 1104 72-73 0.14864310637021916 1104 74-75 0.048875061566420186 1104 76-77 0.18985842919971319 1104 78-79 0.20762004428292968 1104 80-81 0.3637830007292102 1104 82-83 0.2649909304579836 1104 84-85 0.32367212814781254 1104 86-87 0.13811271580291162 1104 88-89 0.22408049886532666 1104 90-91 0.09733564789147664 1104 92-93 0.0016544316679265592 1104 94-95 0.10942520825616597 1104 96-97 0.34701361904488337 1104 98-99 0.21602705561605973 1104 100-101 0.44692855010008614 1105 1 0.11563632478485175 1105 2 0.2280350955678614 1105 3 0.2490579346138162 1105 4 0.3374374608044377 1105 5 0.3347337213118635 1105 6 0.39505599969967875 1105 7 0.4581751510510017 1105 8 -0.030990524327357605 1105 9 0.22619583998582016 1105 10-11 0.3159494823576381 1105 12-13 0.235314156964904 1105 14-15 0.27777020744164105 1105 16-17 0.30791039306131296 1105 18-19 0.1965823669129847 1105 20-21 -0.027133821973123418 1105 22-23 -0.22774913759160142 1105 24-25 -0.1812088265233811 1105 26-27 -0.1778172198939032 1105 28-29 -0.020803344652669864 1105 30-31 -0.07741972812348052 1105 32-33 -0.11173608581822947 1105 34-35 -0.24930902985977355 1105 36-37 -0.39073184535930494 1105 38-39 -0.2877964731985898 1105 40-41 -0.4074124379049451 1105 42-43 -0.33930379067768257 1105 44-45 -0.11821987129550848 1105 46-47 -0.24662841571262817 1105 48-49 -0.11958612494414211 1105 50-51 -0.23114175955956995 1105 52-53 -0.13234113239489176 1105 54-55 0.020964265527368298 1105 56-57 0.0210023567085571 1105 58-59 -0.09570977910905754 1105 60-61 -0.07486234773594447 1105 62-63 -0.33278141183996013 1105 64-65 -0.21170628931497504 1105 66-67 -0.3341570490506882 1105 68-69 -0.3304589630065955 1105 70-71 -0.18599327897969076 1105 72-73 -0.27718139522319873 1105 74-75 -0.375609428967131 1105 76-77 -0.17098879072628748 1105 78-79 -0.2639770516471529 1105 80-81 0.005807355237813283 1105 82-83 0.011810648944493352 1105 84-85 -0.20516020086608933 1105 86-87 -0.0576461962255479 1105 88-89 -0.0757333647334626 1105 90-91 0.014486421226937551 1105 92-93 0.052970071116302364 1105 94-95 -0.018375674252713026 1105 96-97 0.0598312338765048 1105 98-99 -0.15382298058504773 1105 100-101 0.07880063192028786 1106 1 0.0749169886424923 1106 2 0.018780411278502385 1106 3 0.06831232793200925 1106 4 0.18221792306062667 1106 5 0.2104214178039996 1106 6 0.1715219747786918 1106 7 0.3047732131830472 1106 8 0.3127733401022468 1106 9 0.3137753894238955 1106 10-11 0.376213836588704 1106 12-13 0.37605891844475536 1106 14-15 0.3182299557377277 1106 16-17 0.34620268329535264 1106 18-19 0.27544109487018886 1106 20-21 0.29177105955312044 1106 22-23 0.24079992776744774 1106 24-25 0.07775384741849223 1106 26-27 0.11019074994486289 1106 28-29 0.04790055893334255 1106 30-31 0.13348566329509026 1106 32-33 9.601638405669632E-4 1106 34-35 0.0648846128359537 1106 36-37 0.15776057761301132 1106 38-39 0.015204201577390108 1106 40-41 -0.006783870910211931 1106 42-43 0.10483778203531813 1106 44-45 -0.006438725949863056 1106 46-47 -0.013000254691952762 1106 48-49 0.017145174269820984 1106 50-51 0.20720823995547022 1106 52-53 0.11222179427621626 1106 54-55 0.35631026488169226 1106 56-57 0.44296659219568113 1106 58-59 0.3367119600394517 1106 60-61 0.32881615129623043 1106 62-63 0.3681941729081615 1106 64-65 0.34239806680047025 1106 66-67 0.4378233976870405 1106 68-69 0.44266541608172005 1106 70-71 0.3331901727973836 1106 72-73 0.42214223537643747 1106 74-75 0.32111797677553255 1106 76-77 0.11953878156272779 1106 78-79 0.3478780142301048 1106 80-81 0.25667271733757246 1106 82-83 0.3871311675382394 1106 84-85 0.3714011711876566 1106 86-87 0.45547771176660135 1106 88-89 0.5667577779657229 1106 90-91 0.5577075551554245 1106 92-93 0.4089428085037419 1106 94-95 0.31318499808465816 1106 96-97 0.39631576183121453 1106 98-99 0.4721342799075785 1106 100-101 0.5238174736084211 1107 1 0.44044914515209044 1107 2 0.46498900635927853 1107 3 0.5568750044646116 1107 4 0.42815600057765124 1107 5 0.5059170110439553 1107 6 0.4822692614658308 1107 7 0.6575389659488025 1107 8 0.6791315154829789 1107 9 0.7868187481815454 1107 10-11 0.8241800725248609 1107 12-13 0.6756144450679571 1107 14-15 0.8555385953020078 1107 16-17 0.9135326243094966 1107 18-19 1.0131345348192653 1107 20-21 0.939883765560559 1107 22-23 0.9113268945350157 1107 24-25 0.8510879763917174 1107 26-27 0.9164263967820077 1107 28-29 0.8457201439709721 1107 30-31 0.8937745529072885 1107 32-33 0.8449901522314605 1107 34-35 0.7710450355377247 1107 36-37 0.9654511604915612 1107 38-39 0.9359092831847278 1107 40-41 0.7512638457841163 1107 42-43 0.8482055810422153 1107 44-45 0.9022073481097408 1107 46-47 0.8958219451076843 1107 48-49 0.8797725218595787 1107 50-51 0.7048766534133648 1107 52-53 0.8295790402048908 1107 54-55 0.957448753238225 1107 56-57 0.9331711801446261 1107 58-59 0.869442053671797 1107 60-61 0.9007658668549681 1107 62-63 0.9435951573316999 1107 64-65 0.7945620264832343 1107 66-67 0.8235756571235058 1107 68-69 0.8493958062707527 1107 70-71 0.9640002427052003 1107 72-73 0.9777429977441123 1107 74-75 0.8771624892110204 1107 76-77 1.088068330543404 1107 78-79 1.0327026298817863 1107 80-81 1.0943686888681299 1107 82-83 1.0906609154288915 1107 84-85 1.2449345717887255 1107 86-87 1.100106600004512 1107 88-89 1.1085335738768585 1107 90-91 1.1570308995915468 1107 92-93 1.0898658954418643 1107 94-95 1.1809402447872763 1107 96-97 1.4138086281962607 1107 98-99 1.5521076621365992 1107 100-101 1.6845407102715058 1108 1 0.08598673640691601 1108 2 -0.0034473350388779522 1108 3 -0.11503953133021483 1108 4 -0.03195178522673103 1108 5 0.16062021786274272 1108 6 0.05601462705845961 1108 7 0.19719038207080075 1108 8 0.023063786247035978 1108 9 0.15515571259693672 1108 10-11 0.03937519540832568 1108 12-13 0.1362783597566377 1108 14-15 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2316 64-65 -2.0765628790470103 2316 66-67 -2.9944605458892504 2316 68-69 -2.405289771468375 2316 70-71 -2.0513272157709075 2316 72-73 -2.058084092636232 2316 74-75 -2.0992363358175368 2316 76-77 -2.8455012689048225 2316 78-79 -2.9895159236119433 2316 80-81 -2.895552580218432 2316 82-83 -2.854250435861644 2316 84-85 -2.9888845011969636 2316 86-87 -2.950870382392928 2316 88-89 -3.462943040424822 2316 90-91 -3.3358600829184013 2316 92-93 -3.622355372910949 2316 94-95 -3.624289405387234 2316 96-97 -3.7673517172512128 2316 98-99 -4.31516410014936 2316 100-101 -4.882634442164164 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5758.0 3 2482.0 4 2561.0 5 2846.0 6 3257.0 7 3774.0 8 4819.0 9 5994.0 10 7721.0 11 9418.0 12 11630.0 13 13696.0 14 15387.0 15 18522.0 16 22373.0 17 26425.0 18 23417.0 19 17710.0 20 18132.0 21 19709.0 22 21914.0 23 24542.0 24 28782.0 25 32765.0 26 38306.0 27 45442.0 28 54081.0 29 64387.0 30 77438.0 31 94177.0 32 115829.0 33 144691.0 34 188382.0 35 250397.0 36 335277.0 37 415234.0 38 312689.0 39 47226.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.360637474795645 18.369864396114522 14.170596138663036 22.098901990426796 2 15.511545016839987 20.651905939839835 39.19757690972889 24.63897213359129 3 20.921056276439558 24.249759595407973 29.375264642915088 25.453919485237375 4 12.59381323487034 16.090948962433536 37.84528812142362 33.4699496812725 5 13.525340714659539 38.0645158736254 33.52285458433722 14.887288827377837 6 31.507129180464954 36.047378998731425 17.089325788422585 15.356166032381033 7 28.957046813044673 32.88284508560794 20.349548271573763 17.810559829773627 8 26.773025715596344 32.432570423719795 19.425005709255803 21.369398151428065 9 26.34496263311113 17.745868746527275 21.07386470669248 34.83530391366911 10-11 25.746794306522908 25.77228902379184 26.55870604016962 21.922210629515636 12-13 27.604917569432402 23.516740653474926 26.749873125885248 22.128468651207424 14-15 23.527773951946504 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29.005017147576396 22.145650225847817 40-41 22.526213321382887 26.57411007270184 28.677249933471238 22.222426672444033 42-43 22.15718706225912 26.687434215212214 27.664254228032824 23.491124494495843 44-45 22.724829280211935 25.896225715153427 27.89623165065236 23.482713353982273 46-47 23.66029844131485 26.73063910443863 27.49741801965075 22.111644434595767 48-49 23.315747154726978 26.64404983590366 27.476580559945614 22.563622449423743 50-51 21.971918597726376 26.991519706167377 28.356019599921332 22.680542096184915 52-53 23.073469850479555 27.48625493361275 26.766678029795663 22.673597186112026 54-55 22.29063754325311 26.50195547875347 27.505366264227355 23.702040713766067 56-57 23.189452407784938 26.523305433502593 27.055765894672863 23.231476264039607 58-59 24.25590992530312 26.122975830056582 26.830908530449555 22.790205714190744 60-61 23.04536470420646 26.688588290924486 28.353306148936465 21.91274085593259 62-63 22.41904328896154 26.965613887637492 28.01894689649701 22.596395926903963 64-65 22.054731125545313 27.80931261313613 27.315564741317072 22.820391520001486 66-67 22.718638231461004 26.96395568799413 26.55050174595603 23.766904334588844 68-69 22.391802226133272 26.786372526614766 27.376710384038162 23.445114863213796 70-71 23.385260433567872 26.737358227737896 26.948126983624466 22.929254355069766 72-73 23.070833690297814 26.17010318763181 28.12681594344179 22.632247178628585 74-75 22.155740870592 28.252492572537463 26.911631794129892 22.68013476274065 76-77 22.619243823756072 26.859461700016517 26.854258153294904 23.667036322932507 78-79 22.9558791833749 27.013493318059496 27.124761684415443 22.90586581415016 80-81 23.453610389569267 27.079039868028755 26.76975205126551 22.69759769113647 82-83 23.117360075944667 26.823738486322085 27.50394423970847 22.554957198024773 84-85 22.62320726608907 27.441128639559253 27.075236107728163 22.860427986623513 86-87 22.725562109665304 27.47993864656798 26.80542287726385 22.98907636650286 88-89 22.419364189126757 27.514481769600323 26.107825448303196 23.95832859296973 90-91 22.850659975396486 26.70354226139842 26.70354226139842 23.742255501806678 92-93 23.538351153484953 27.050827897979318 26.988714277742666 22.422106670793063 94-95 22.883923780338204 26.66991688207258 27.27288196494516 23.173277372644055 96-97 21.930469677606744 26.819661154979187 28.122545961561634 23.127323205852434 98-99 22.38701382535718 27.425829431389815 26.56719406971868 23.619962673534324 100-101 22.09466790860584 26.595556016510454 27.147916776352382 24.161859298531326 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 320.0 1 247.5 2 258.0 3 337.5 4 297.5 5 311.0 6 507.0 7 957.5 8 1535.0 9 1900.0 10 1875.0 11 1720.5 12 1772.0 13 1962.0 14 2209.0 15 2437.5 16 2606.5 17 2759.5 18 2984.5 19 3363.0 20 3839.0 21 4272.5 22 5001.5 23 6080.0 24 7292.5 25 8994.5 26 11727.5 27 15061.0 28 17551.0 29 20609.5 30 25074.5 31 30402.0 32 37624.0 33 43273.5 34 47989.5 35 52737.5 36 58629.5 37 64650.5 38 66372.0 39 66506.0 40 66990.0 41 69707.5 42 72368.0 43 75400.5 44 78972.0 45 82955.0 46 86226.5 47 103289.0 48 142549.5 49 141340.0 50 105023.5 51 87492.5 52 81180.0 53 80370.5 54 79685.0 55 75216.0 56 68383.0 57 61834.5 58 57452.0 59 52082.0 60 45097.0 61 38657.5 62 33366.0 63 26759.0 64 20414.0 65 16484.0 66 12502.0 67 9639.0 68 7159.0 69 4979.5 70 3974.5 71 3274.0 72 2806.5 73 2585.5 74 2068.5 75 1611.5 76 1213.0 77 738.5 78 453.0 79 321.0 80 217.5 81 146.0 82 91.5 83 55.0 84 42.0 85 33.5 86 29.5 87 21.0 88 12.0 89 11.5 90 10.5 91 8.5 92 5.5 93 3.0 94 1.5 95 1.0 96 1.5 97 0.5 98 1.5 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01416585846091509 2 0.007201637541582533 3 0.008428290089874063 4 0.035889479396787674 5 0.04704806064253643 6 0.06042253036261828 7 0.02710506437353865 8 0.02389993997316401 9 0.01452198339429005 10-11 0.047067845361057264 12-13 0.04261628369387026 14-15 0.020536537824622714 16-17 0.02291070404712245 18-19 0.0308245914554549 20-21 0.024533050965830604 22-23 0.014225212616477583 24-25 0.0029677077781246697 26-27 0.0029677077781246697 28-29 0.0013651455779373483 30-31 0.006667450141520091 32-33 0.0016025622001873219 34-35 0.0017806246668748017 36-37 0.00627175577110347 38-39 0.006707019578561754 40-41 0.003976728422687058 42-43 9.100970519582322E-4 44-45 0.0014047150149790104 46-47 0.0028885689040413455 48-49 0.0011475136742082057 50-51 0.001978471852083113 52-53 9.298817704790633E-4 54-55 0.002868784185520514 56-57 0.002651152281791372 58-59 0.0022158884743330868 60-61 7.122498667499207E-4 62-63 0.0013651455779373483 64-65 0.004095436733812045 66-67 0.0030666313707288255 68-69 0.006944436200811727 70-71 0.010406761941957176 72-73 0.0042537144819786935 74-75 0.0037590965189579152 76-77 0.003185339681853812 78-79 0.034761750441100296 80-81 0.02342510672866406 82-83 0.003956943704166226 84-85 0.009160324675144814 86-87 0.008111734593540764 88-89 0.0057969225266035216 90-91 0.012998560068186053 92-93 0.015115524949914983 94-95 0.007557762474957492 96-97 0.004688978289436978 98-99 0.022851349891559958 100-101 0.041389631145578726 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2527203.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.90466674750457 #Duplication Level Percentage of deduplicated Percentage of total 1 76.03496512219273 36.42429665336776 2 13.235033070393474 12.68039697258803 3 4.567434251092877 6.564042470692271 4 1.9982262240496902 3.8289744539689927 5 1.0462072627401469 2.5059105135192845 6 0.6488269327840741 1.8649102795095969 7 0.43750961314756714 1.4671126551664695 8 0.29513761707140485 1.131077535236661 9 0.222667281900721 0.9600121741524026 >10 1.2765153060700856 11.707329471663105 >50 0.1377877369772931 4.60964620166392 >100 0.09113953164719077 7.890887705564855 >500 0.005229642191810245 1.7014176607150762 >1k 0.0024072956121028617 2.3832413229086002 >5k 6.640815481663067E-4 2.2372612305306143 >10k+ 2.4903058056236497E-4 2.043482698752372 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 26154 1.0348990563876348 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 15023 0.5944516526768923 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10211 0.4040435216324134 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 9464 0.37448515216229167 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8422 0.3332537987648796 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 8027 0.317623871133423 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6658 0.2634533118233874 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6272 0.24817950912530573 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6185 0.24473696810268109 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5970 0.23622953913872372 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5263 0.2082539471502685 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4937 0.1953543106746866 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4899 0.19385067206710344 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4080 0.16144330312998204 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3990 0.15788205379623244 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2911 0.11518663122827885 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2839 0.11233763176127917 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2768 0.10952820173132116 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2631 0.10410718885661342 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.9784718520831133E-5 0.0 16-17 0.0 0.0 0.0 3.9569437041662266E-5 0.0 18-19 0.0 0.0 0.0 3.9569437041662266E-5 0.0 20-21 0.0 0.0 0.0 7.913887408332453E-5 0.0 22-23 0.0 0.0 0.0 9.892359260415566E-5 0.0 24-25 0.0 0.0 0.0 1.3849302964581792E-4 0.0 26-27 0.0 0.0 0.0 1.9784718520831133E-4 0.0 28-29 0.0 0.0 0.0 3.9569437041662265E-4 0.0 30-31 0.0 0.0 0.0 6.726804297082585E-4 0.0 32-33 0.0 0.0 0.0 0.001325576140895686 0.0 34-35 0.0 0.0 0.0 0.0018993329779997885 0.0 36-37 0.0 0.0 0.0 0.0029677077781246697 0.0 38-39 0.0 0.0 0.0 0.0038580201115620705 0.0 40-41 0.0 0.0 0.0 0.005519936467311886 0.0 42-43 0.0 0.0 0.0 0.007597331911999155 0.0 44-45 0.0 0.0 0.0 0.01254351154220694 0.0 46-47 0.0 0.0 1.9784718520831133E-5 0.02389993997316401 0.0 48-49 0.0 0.0 3.9569437041662266E-5 0.03151705660368399 0.0 50-51 0.0 0.0 3.9569437041662266E-5 0.041429200582620396 0.0 52-53 0.0 0.0 3.9569437041662266E-5 0.055120225799035534 0.0 54-55 0.0 0.0 3.9569437041662266E-5 0.0731441043715127 0.0 56-57 0.0 0.0 3.9569437041662266E-5 0.09241442021080222 0.0 58-59 0.0 0.0 3.9569437041662266E-5 0.12406996984413202 1.9784718520831133E-5 60-61 0.0 0.0 3.9569437041662266E-5 0.1648067052785233 3.9569437041662266E-5 62-63 0.0 0.0 3.9569437041662266E-5 0.21274507825449718 3.9569437041662266E-5 64-65 0.0 0.0 3.9569437041662266E-5 0.24928745336247227 3.9569437041662266E-5 66-67 0.0 0.0 3.9569437041662266E-5 0.31107512930302794 3.9569437041662266E-5 68-69 0.0 0.0 3.9569437041662266E-5 0.38677146236372784 3.9569437041662266E-5 70-71 0.0 0.0 3.9569437041662266E-5 0.438528286014222 3.9569437041662266E-5 72-73 0.0 0.0 3.9569437041662266E-5 0.5248094434835666 3.9569437041662266E-5 74-75 0.0 0.0 3.9569437041662266E-5 0.5944516526768922 3.9569437041662266E-5 76-77 0.0 0.0 3.9569437041662266E-5 0.657030717358281 3.9569437041662266E-5 78-79 0.0 0.0 3.9569437041662266E-5 0.7420654375608133 3.9569437041662266E-5 80-81 0.0 0.0 3.9569437041662266E-5 0.8310966709045533 3.9569437041662266E-5 82-83 0.0 0.0 3.9569437041662266E-5 0.9479254337700612 3.9569437041662266E-5 84-85 0.0 0.0 3.9569437041662266E-5 1.1068165082108559 3.9569437041662266E-5 86-87 0.0 0.0 3.9569437041662266E-5 1.3258729116734984 3.9569437041662266E-5 88-89 0.0 0.0 3.9569437041662266E-5 1.6037492832985716 3.9569437041662266E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12835 0.0 26.214897 1 TACATGG 13210 0.0 24.605356 2 ACATGGG 13275 0.0 23.73315 3 GAGTACT 8925 0.0 23.159172 12-13 GTACTTT 9470 0.0 21.74873 14-15 ATGGGCG 460 0.0 21.69563 5 AGTACTT 9255 0.0 20.536459 12-13 CATGGGG 6155 0.0 20.305454 4 GCGCGAC 120 0.0047545517 19.799213 9 CATGGGA 6640 0.0 19.537975 4 ATTCGCG 500 0.0 19.47645 80-81 ACTTTTT 11000 0.0 19.457361 16-17 AGAGTAC 17015 0.0 19.199755 10-11 GTATAGG 860 0.0 18.788464 1 GATGTGC 1220 0.0 17.916666 8 CGCGGAA 535 0.0 17.75587 82-83 GTACCTG 1870 0.0 17.281368 1 ATACCGT 220 2.9069177E-5 17.281366 6 GTAATAC 330 1.2058081E-8 17.279999 3 TACTTTT 10955 0.0 17.13087 14-15 >>END_MODULE