FastQCFastQC Report
Thu 26 May 2016
SRR936746_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936746_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2527203
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT268491.06239981513159No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT147490.5836096269274768No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT105820.4187237827748701No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC95530.3780068320589996No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84030.332501979461088No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT75400.2983535552941335No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73260.2898856957672178No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC66780.2642447005642206No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC66250.2621475204010125No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT63450.2510680780293471No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG53670.21236916860260138No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC51450.20358475357935235No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA51170.20247680934218582No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA43280.17125652351631426No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43170.170821259708856No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC32500.12860067038540235No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT32330.1279279899556941No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA31440.12440631005898617No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC27700.10960734060540447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG125500.023.354951
TACATGG131100.021.8109112
ACATGGG130750.021.1773933
GAGTACT96050.020.91234412-13
AGTACTT100650.019.81505412-13
GTACTTT101750.019.4608314-15
ATACCGT2702.30757E-819.3478016
CATGGGG55850.019.2182454
AGAGTAC177900.018.56476810-11
TAGCCCG1300.00757471218.2654065
CATGGGA68650.017.4336574
TACTTTT113700.017.31106414-15
GATATAG10050.017.1837481
ACTTTTT118050.016.97519516-17
GAGTACA112600.016.6152821
GTACTAG3502.2178938E-816.4473021
GTGTAAG11700.016.4004441
CGCGGAA4800.016.32890782-83
GCACCGT8200.016.2161166
AGTACAT103000.016.1863862
Produced by FastQC (version 0.11.5)