##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936746_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2527203 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.438036042217423 31.0 31.0 34.0 30.0 34.0 2 31.63699077596853 31.0 31.0 34.0 30.0 34.0 3 31.913232138455044 31.0 31.0 34.0 30.0 34.0 4 35.715571720989566 37.0 35.0 37.0 33.0 37.0 5 35.51963811375659 37.0 35.0 37.0 33.0 37.0 6 35.62859058017896 37.0 35.0 37.0 33.0 37.0 7 35.558325152352225 37.0 35.0 37.0 33.0 37.0 8 35.53777911786271 37.0 35.0 37.0 33.0 37.0 9 37.14617266598686 39.0 37.0 39.0 33.0 39.0 10-11 37.02549320335565 39.0 37.0 39.0 33.0 39.0 12-13 36.90376238078223 39.0 37.0 39.0 33.0 39.0 14-15 37.980187385026056 40.0 37.0 41.0 33.0 41.0 16-17 37.91067951407149 40.0 37.0 41.0 33.0 41.0 18-19 37.983940348282275 40.0 37.0 41.0 33.0 41.0 20-21 38.05562018563606 40.0 37.0 41.0 33.0 41.0 22-23 37.97130365071583 40.0 37.0 41.0 33.0 41.0 24-25 37.842812983365405 40.0 37.0 41.0 32.5 41.0 26-27 37.62048260468194 39.0 37.0 41.0 32.0 41.0 28-29 37.5556249339685 39.0 36.5 41.0 32.0 41.0 30-31 37.57589081684376 39.0 37.0 41.0 32.0 41.0 32-33 37.41113396905591 39.0 36.0 41.0 32.0 41.0 34-35 37.22299554092014 39.0 36.0 41.0 31.0 41.0 36-37 36.97996401555396 39.0 35.5 41.0 31.0 41.0 38-39 36.8300621279731 39.0 35.0 41.0 31.0 41.0 40-41 36.63638200017965 39.0 35.0 40.5 30.0 41.0 42-43 36.387877428129045 39.0 35.0 40.0 30.0 41.0 44-45 36.103923388821556 39.0 35.0 40.0 30.0 41.0 46-47 35.72215904302108 38.0 35.0 40.0 28.5 41.0 48-49 35.27803227520702 38.0 34.0 40.0 27.0 41.0 50-51 35.07442635197884 38.0 34.0 40.0 26.5 41.0 52-53 34.82310305108058 38.0 34.0 40.0 26.0 41.0 54-55 34.54982049324886 38.0 33.5 40.0 25.5 41.0 56-57 34.239336927029605 37.5 33.0 40.0 24.5 41.0 58-59 33.869498216011934 37.0 33.0 40.0 23.5 41.0 60-61 33.44722980306687 36.5 32.0 39.0 23.0 41.0 62-63 33.182473469681696 36.0 32.0 39.0 22.5 40.0 64-65 32.76159829661488 35.5 31.5 39.0 22.0 40.0 66-67 32.31483422582198 35.0 31.0 38.5 21.0 40.0 68-69 31.839658903538812 35.0 31.0 38.0 20.0 40.0 70-71 31.337578936080718 34.5 30.0 37.0 18.0 39.0 72-73 30.920484622723222 34.0 30.0 36.5 18.0 39.0 74-75 30.566057811738908 34.0 29.5 36.0 15.5 39.0 76-77 29.125463803263926 32.5 28.0 35.0 12.5 36.5 78-79 29.948621658014808 34.0 29.0 35.0 12.5 37.0 80-81 29.772372262932578 34.0 29.0 35.0 7.5 37.0 82-83 29.422596839272508 34.0 29.0 35.0 7.0 36.5 84-85 28.96306509607657 34.0 29.0 35.0 3.5 36.0 86-87 28.626252026449794 33.0 29.0 35.0 2.0 36.0 88-89 28.209307483411504 33.0 27.5 35.0 2.0 35.0 90-91 27.730473175285088 33.0 27.0 35.0 2.0 35.0 92-93 27.26894713246225 33.0 26.0 34.5 2.0 35.0 94-95 26.702179445022814 32.0 25.0 34.0 2.0 35.0 96-97 25.991270586494238 32.0 24.5 34.0 2.0 35.0 98-99 25.133702753597554 32.0 21.0 34.0 2.0 35.0 100-101 22.321431440212756 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.37371280095504744 1101 2 0.377971963373799 1101 3 0.19119419139362392 1101 4 0.17096380643698694 1101 5 0.1661413700954597 1101 6 0.06825399191561843 1101 7 0.14880158845822677 1101 8 0.17083636938129132 1101 9 0.19645502421658279 1101 10-11 0.30092337415714354 1101 12-13 0.2094272350429236 1101 14-15 0.3622720419338634 1101 16-17 0.23764700922865956 1101 18-19 0.2693437264589633 1101 20-21 -0.01580933075191382 1101 22-23 0.10479188885015134 1101 24-25 0.09500104152623123 1101 26-27 0.13114658511551625 1101 28-29 0.01630373528745821 1101 30-31 0.009832190433954224 1101 32-33 0.18598156295598756 1101 34-35 0.22871001921532041 1101 36-37 0.24274854194393214 1101 38-39 0.11493254724199176 1101 40-41 0.17826817428920094 1101 42-43 0.31299801849556985 1101 44-45 0.19593776951271735 1101 46-47 0.21953409164303395 1101 48-49 0.26942405239924483 1101 50-51 0.18552468847291692 1101 52-53 0.25012398406297365 1101 54-55 0.11124776946186898 1101 56-57 0.17553802230802518 1101 58-59 0.15252699678310222 1101 60-61 0.09811538686330579 1101 62-63 -0.18767273738544787 1101 64-65 -0.1915473010856985 1101 66-67 -0.3949236239066174 1101 68-69 -0.5650414347329651 1101 70-71 -0.44496004930928024 1101 72-73 -0.40340362017876785 1101 74-75 -0.14071012449293718 1101 76-77 0.1875953565326185 1101 78-79 0.07269559245991886 1101 80-81 0.11849532995911005 1101 82-83 -0.02232877849073489 1101 84-85 -0.0288798044779206 1101 86-87 -0.15015840790234947 1101 88-89 -0.12865118605884973 1101 90-91 -0.11169133765659822 1101 92-93 -0.22211583587927564 1101 94-95 -0.363014348876856 1101 96-97 -0.30443187721529696 1101 98-99 -0.3711172105123417 1101 100-101 -0.6419192315365265 1102 1 0.4401408855503597 1102 2 0.5041563457049456 1102 3 0.2022296254245326 1102 4 0.1209993708369197 1102 5 0.21607642200327604 1102 6 0.1996163212115647 1102 7 0.1560225463936149 1102 8 -0.023629296618672413 1102 9 0.18124744362927458 1102 10-11 0.2642776881222417 1102 12-13 0.2038369031558105 1102 14-15 0.29667041590112575 1102 16-17 0.45582700359509687 1102 18-19 0.35175907271706564 1102 20-21 0.34704920939871187 1102 22-23 0.19353486125041997 1102 24-25 0.30688330005813214 1102 26-27 0.011613556156703453 1102 28-29 0.12384889329404558 1102 30-31 0.22500373378030503 1102 32-33 0.3946697803655823 1102 34-35 0.4407126833367485 1102 36-37 0.36984618294005855 1102 38-39 0.34824750732407495 1102 40-41 0.4518901297840614 1102 42-43 0.36504548768153455 1102 44-45 0.23765737748698257 1102 46-47 0.26763806668243006 1102 48-49 0.22868187437190102 1102 50-51 0.1356963635392674 1102 52-53 0.08997965271760222 1102 54-55 0.31804800020828594 1102 56-57 0.4021778180947351 1102 58-59 0.34728582421841736 1102 60-61 0.3621013488887712 1102 62-63 0.2772091908332257 1102 64-65 0.25751370418783637 1102 66-67 0.32784292840006657 1102 68-69 0.34430244430619084 1102 70-71 0.15186597153760317 1102 72-73 0.05129111703307743 1102 74-75 -0.2366982034952123 1102 76-77 0.25284927554035264 1102 78-79 0.513914022722286 1102 80-81 0.5168150893775945 1102 82-83 0.46560381194180067 1102 84-85 0.6759856621182259 1102 86-87 0.4831511820566696 1102 88-89 0.5043684510289062 1102 90-91 0.3201847671871185 1102 92-93 0.4495355884192165 1102 94-95 0.20127362346599398 1102 96-97 0.055974475606522134 1102 98-99 -0.20824796388994926 1102 100-101 0.14997583423228633 1103 1 0.5434580575086478 1103 2 0.5456004916289849 1103 3 0.35893645778331873 1103 4 0.18633693912492788 1103 5 0.08136982387964764 1103 6 0.18018980164287512 1103 7 0.2071241058445068 1103 8 0.22934722912501826 1103 9 0.09403824705906061 1103 10-11 0.14394368282545855 1103 12-13 0.2047062698241433 1103 14-15 0.30426734186782056 1103 16-17 0.3092378377052256 1103 18-19 0.3076582405440078 1103 20-21 0.20608910692242688 1103 22-23 0.1762416687177435 1103 24-25 0.12614047781805482 1103 26-27 0.15436456833023726 1103 28-29 0.29024935946669217 1103 30-31 0.27547726217557056 1103 32-33 0.17680307868065626 1103 34-35 0.24572619334423962 1103 36-37 0.23020429148157007 1103 38-39 0.33873688967403837 1103 40-41 0.4099305229034158 1103 42-43 0.310532998384069 1103 44-45 0.3189120067428277 1103 46-47 0.29799433708426903 1103 48-49 0.35263232374279596 1103 50-51 0.1506507945887634 1103 52-53 0.28099156456476493 1103 54-55 0.28561823804633235 1103 56-57 0.19785550923813133 1103 58-59 0.2677159169902197 1103 60-61 0.3544350140020569 1103 62-63 0.19144768881782426 1103 64-65 0.1399882869604454 1103 66-67 0.16666241191473574 1103 68-69 0.21193357052096573 1103 70-71 0.05734985063779874 1103 72-73 0.14260828452366425 1103 74-75 0.14477234334814781 1103 76-77 0.06702069419166534 1103 78-79 0.12082131123330697 1103 80-81 0.027526720685074224 1103 82-83 0.26348407948047736 1103 84-85 0.3075292877785323 1103 86-87 0.2567129574315281 1103 88-89 0.3529932608723989 1103 90-91 0.2623609139421461 1103 92-93 0.30173320247562074 1103 94-95 0.360635706027189 1103 96-97 0.04783735676919321 1103 98-99 0.026254938998516053 1103 100-101 0.2564613239385416 1104 1 0.27636757178717986 1104 2 0.3513691342674612 1104 3 0.0632630154621765 1104 4 0.05530629879378779 1104 5 -0.060638678744119545 1104 6 0.0805590662325315 1104 7 0.007787237825937154 1104 8 -0.02433799817179505 1104 9 0.01850494057399743 1104 10-11 0.010395336264814148 1104 12-13 -0.023293039791873582 1104 14-15 0.13838631501248955 1104 16-17 -0.03426975197655935 1104 18-19 0.07384872899067574 1104 20-21 0.035415664601281094 1104 22-23 -0.01785426134757273 1104 24-25 0.09289795924884459 1104 26-27 0.18236917694610355 1104 28-29 0.010336429604251407 1104 30-31 -0.017050088233666827 1104 32-33 -0.04561290189169398 1104 34-35 -0.039899815358232615 1104 36-37 9.532067005935119E-4 1104 38-39 -0.06980396258681765 1104 40-41 0.11163339435573505 1104 42-43 -0.07586053259691283 1104 44-45 0.09260232107949662 1104 46-47 0.12073264824758922 1104 48-49 0.018378680301900374 1104 50-51 0.05117418437411203 1104 52-53 0.060072324350507245 1104 54-55 0.18337812370905482 1104 56-57 0.15980664640349573 1104 58-59 0.14951233849992462 1104 60-61 -0.00833262334044349 1104 62-63 -0.255630896863984 1104 64-65 -0.038699287036301655 1104 66-67 0.05245541481252758 1104 68-69 -0.009640398974955389 1104 70-71 -0.02420675327861943 1104 72-73 -0.08017870173830133 1104 74-75 -0.16493602998001933 1104 76-77 0.07450689408205236 1104 78-79 0.04567278783746431 1104 80-81 0.09781878641689445 1104 82-83 0.06451465925162125 1104 84-85 0.19535074781952844 1104 86-87 0.2788272735123307 1104 88-89 0.13232746425104125 1104 90-91 -0.03527005462418842 1104 92-93 -0.12631190563911332 1104 94-95 -0.07648272813349166 1104 96-97 -0.2218426744797668 1104 98-99 -0.03546864397322125 1104 100-101 0.12305423357393863 1105 1 0.35611034439292055 1105 2 0.3873558852323491 1105 3 0.11387073798496417 1105 4 0.10022132595887001 1105 5 -0.09662286427621325 1105 6 -0.018062726263856632 1105 7 0.005876275446581758 1105 8 -0.030012165403810798 1105 9 -0.01480549389629715 1105 10-11 0.041222004223691044 1105 12-13 0.004101506668114041 1105 14-15 0.03452548118982435 1105 16-17 0.07182671399725393 1105 18-19 0.024348275764218386 1105 20-21 0.08238261042503581 1105 22-23 -0.016674874885076463 1105 24-25 -0.09659274533301954 1105 26-27 -0.05321565621525082 1105 28-29 -0.11893729637332484 1105 30-31 -0.11648455282784198 1105 32-33 -0.10091280098466626 1105 34-35 -0.11819269086633 1105 36-37 -0.08936798548452174 1105 38-39 -0.10023156322304771 1105 40-41 -0.10047616102625767 1105 42-43 -0.09778700159223064 1105 44-45 -0.33186848714584727 1105 46-47 0.02684436429408521 1105 48-49 0.04912339693559886 1105 50-51 -0.16734510482998388 1105 52-53 -0.09740562717991708 1105 54-55 -0.12107546761179577 1105 56-57 -0.12021134013082957 1105 58-59 0.02076409867186868 1105 60-61 -0.1274991142274544 1105 62-63 -0.1523631055875896 1105 64-65 -0.07129311061434151 1105 66-67 -0.21380358558084112 1105 68-69 -0.3150624411734775 1105 70-71 -0.33516941768010255 1105 72-73 -0.5813139057472192 1105 74-75 -0.44826385238365773 1105 76-77 -0.36232174640960224 1105 78-79 -0.30253696764828675 1105 80-81 -0.49843325880313216 1105 82-83 -0.22214737063282186 1105 84-85 -0.08615747011683439 1105 86-87 -0.32150951080318535 1105 88-89 -0.302965764716685 1105 90-91 -0.5450995103533707 1105 92-93 -0.2892629229757411 1105 94-95 0.017115376866819076 1105 96-97 -0.08109077463426217 1105 98-99 0.03202632492802948 1105 100-101 0.3083763949225613 1106 1 0.2670868851891548 1106 2 0.3041612712471675 1106 3 0.11186234415965046 1106 4 0.010473487112555802 1106 5 -0.12635055683330876 1106 6 -0.05641993077917817 1106 7 0.02380785842671429 1106 8 -0.015078555324564036 1106 9 -0.07952717997721237 1106 10-11 -0.02176168337857831 1106 12-13 -0.04820062947590742 1106 14-15 0.04943200323919683 1106 16-17 0.08052120951560227 1106 18-19 -0.05221912354937075 1106 20-21 -0.06974196402963173 1106 22-23 -0.072291726826478 1106 24-25 -0.12697733993397975 1106 26-27 -0.07005685939486739 1106 28-29 -0.02488606327909082 1106 30-31 -0.17007236584699825 1106 32-33 -0.14137123298262821 1106 34-35 -0.3742010375820328 1106 36-37 -0.2532948353336977 1106 38-39 -0.10599092175633018 1106 40-41 0.004738735034685249 1106 42-43 -0.25343350488090266 1106 44-45 -0.09688627102632807 1106 46-47 -0.2328561293869882 1106 48-49 -0.1785947933346037 1106 50-51 -0.12574731288544427 1106 52-53 -0.08176086971824503 1106 54-55 -0.10835947348110864 1106 56-57 -0.043437628405662565 1106 58-59 -0.20758307426615374 1106 60-61 -0.02645743745762985 1106 62-63 -0.35758319756218526 1106 64-65 -0.014167711741372102 1106 66-67 -0.0921490265476379 1106 68-69 -0.042728603028265866 1106 70-71 -0.0112827634189685 1106 72-73 0.06751174848758978 1106 74-75 0.14768700674334312 1106 76-77 0.042464590692961934 1106 78-79 0.12060126720978204 1106 80-81 0.028061744113507814 1106 82-83 0.20127130500499746 1106 84-85 0.32732294683098573 1106 86-87 0.22006812099636264 1106 88-89 0.1707885669433331 1106 90-91 0.0782207893192215 1106 92-93 0.322279279620556 1106 94-95 0.24464630640884621 1106 96-97 0.298440858780868 1106 98-99 0.22533274111932755 1106 100-101 0.2769308640318151 1107 1 0.6176573951581616 1107 2 0.5995946599136381 1107 3 0.42449168084914035 1107 4 0.33031783101704804 1107 5 0.055151538954497425 1107 6 0.2875699139625496 1107 7 0.15687284412327784 1107 8 0.28468094443493186 1107 9 0.41642988440090534 1107 10-11 0.4662704460069378 1107 12-13 0.45116792177234544 1107 14-15 0.6047683961695753 1107 16-17 0.6155423843864014 1107 18-19 0.5246682608568136 1107 20-21 0.4932817568888268 1107 22-23 0.5515232218005011 1107 24-25 0.5492775107569585 1107 26-27 0.5462852223607371 1107 28-29 0.6087684192475749 1107 30-31 0.5461427751801722 1107 32-33 0.6107030363848693 1107 34-35 0.5462953693805446 1107 36-37 0.7921470522134442 1107 38-39 0.7292931759337833 1107 40-41 0.8551913456376781 1107 42-43 0.8375027101154657 1107 44-45 0.7448931414297775 1107 46-47 0.9230234685978687 1107 48-49 0.8219856621330734 1107 50-51 0.7214753614048988 1107 52-53 0.5788155352190643 1107 54-55 0.7520476771516087 1107 56-57 0.7583167575592498 1107 58-59 0.610242514236127 1107 60-61 0.631571473202861 1107 62-63 0.45047374132181517 1107 64-65 0.5106772288930443 1107 66-67 0.5586750032237617 1107 68-69 0.6340217143235556 1107 70-71 0.6636650679198404 1107 72-73 0.7500673438251404 1107 74-75 1.0084178381508657 1107 76-77 0.7888511197862584 1107 78-79 0.8205589536336326 1107 80-81 0.8536328869778842 1107 82-83 0.8400403054281291 1107 84-85 0.9276882178729515 1107 86-87 1.120748393105206 1107 88-89 1.260289013586636 1107 90-91 1.03460815021786 1107 92-93 1.242889246095185 1107 94-95 1.2804537181561066 1107 96-97 1.522918720100833 1107 98-99 1.5898736026526699 1107 100-101 1.5325884061555222 1108 1 0.28212630162913044 1108 2 0.30132229053703696 1108 3 0.03731131162542667 1108 4 0.034858594690938105 1108 5 -0.08094141674629896 1108 6 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-1.2840803164416101 2316 52-53 -1.3900513902152483 2316 54-55 -1.039701107887801 2316 56-57 -1.0412209826507492 2316 58-59 -1.1682893095377267 2316 60-61 -1.1416502329515552 2316 62-63 -1.060522171714645 2316 64-65 -0.9736597462647723 2316 66-67 -0.7996658510029846 2316 68-69 -1.0634864852846242 2316 70-71 -1.0584329066974476 2316 72-73 -0.9799272019985033 2316 74-75 -1.1317246552637634 2316 76-77 -1.2528564532030941 2316 78-79 -1.0575893657218423 2316 80-81 -0.9387968140696614 2316 82-83 -0.7235380255939035 2316 84-85 -1.0691483598392786 2316 86-87 -0.9762403145484946 2316 88-89 -1.1197147040582358 2316 90-91 -1.1817746791544685 2316 92-93 -1.3516781007537446 2316 94-95 -1.376953322378455 2316 96-97 -1.5614444253202286 2316 98-99 -1.9853857153062435 2316 100-101 -2.5149311665109835 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 21.0 4 40.0 5 104.0 6 238.0 7 560.0 8 1157.0 9 2162.0 10 3425.0 11 5385.0 12 7411.0 13 10105.0 14 12991.0 15 16893.0 16 20991.0 17 25983.0 18 22387.0 19 16470.0 20 15997.0 21 16627.0 22 17692.0 23 19882.0 24 24091.0 25 28941.0 26 35471.0 27 43975.0 28 54647.0 29 67532.0 30 82521.0 31 101122.0 32 123640.0 33 156447.0 34 201954.0 35 268041.0 36 369671.0 37 462328.0 38 273788.0 39 16511.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.615580118445344 19.353505806379427 14.904172760767578 22.126741314407653 2 15.597060760327231 21.378623394878808 38.29088626750753 24.73342957728643 3 20.939997435787856 24.295197800475805 29.720999472911945 25.043805290824395 4 13.010140984417513 16.805580014840466 37.25380163245115 32.93047736829087 5 13.393700466484093 38.317380914789986 33.59520386767505 14.693714751050866 6 31.64407449658773 36.38445348474183 16.6383943039004 15.333077714770043 7 28.654761805838312 33.14280649397773 20.196794638183 18.005637062000957 8 26.78174250347123 32.84239532795743 19.172302343737325 21.203559824834016 9 25.946752130021643 17.606793909350966 21.04316155320335 35.40329240742404 10-11 25.777466937190308 25.937070608846362 26.397679643162043 21.887782810801294 12-13 27.46856399854701 23.605787912146177 26.80626923573064 22.119378853576173 14-15 23.31148309019893 25.304298863209645 26.63723491939508 24.74698312719635 16-17 22.0710603778169 28.62462967953109 26.91956285268734 22.384747089964677 18-19 21.892839633381254 27.71890505036596 28.83068752292554 21.557567793327248 20-21 23.217663954973148 25.609517715830503 28.57823451459974 22.59458381459661 22-23 22.402709753112923 25.72007989181564 29.023462038032697 22.85374831703874 24-25 23.379522737192065 26.00683443316584 28.74124081049286 21.872402019149234 26-27 22.67746200048037 25.635890745618774 29.90604632868828 21.780600925212575 28-29 21.609621387755553 27.406820900418367 29.127082391086113 21.856475320739964 30-31 22.106459612951475 25.83744278505581 29.064706923960387 22.99139067803233 32-33 22.168495484032317 26.142495603141718 29.198304689368815 22.490704223457154 34-35 23.545141873101645 25.79622093805447 28.656277253611524 22.00235993523236 36-37 23.07623971925293 25.33941378596023 29.49522073495285 22.08912575983399 38-39 22.349622922154467 26.415293307169957 29.24528637854508 21.98979739213049 40-41 22.462971107957316 26.55429740124557 28.85350070208532 22.12923078871179 42-43 22.179937936879078 26.673330844160148 27.860417140312 23.286314078648772 44-45 22.731332304235668 25.88110716768765 28.033486959332965 23.35407356874371 46-47 23.596264341947684 26.813870202442786 27.653647367191255 21.936218088418276 48-49 23.448817604500256 26.616428584855168 27.49847396137861 22.43627984926597 50-51 22.058720532005694 26.93720023536013 28.468208378393246 22.535870854240933 52-53 23.13104017543936 27.504895848708085 26.839677722770517 22.524386253082042 54-55 22.356641329522372 26.538526606969544 27.57035484863505 23.534477214873032 56-57 23.27387189601246 26.576844664497095 27.063376112750248 23.085907326740195 58-59 24.279224495989858 26.179700386580727 26.858684849948943 22.682390267480468 60-61 23.080055361377426 26.709430923425003 28.37773158833533 21.832782126862238 62-63 22.46457215124957 26.894279943714317 28.07193044381166 22.56921746122445 64-65 22.179404270678404 27.777239090393152 27.311635093429498 22.731721545498942 66-67 22.80315691607707 26.967082448503444 26.613691300238436 23.61606933518105 68-69 22.449479461378054 26.795947277410882 27.353325234746894 23.401248026464174 70-71 23.456231254833753 26.738246598987043 26.940133980887175 22.865388165292032 72-73 23.17633367798313 26.170394701177546 28.126430682458036 22.526840938381284 74-75 22.314491554497202 28.183291963486905 26.856726586665182 22.64548989535071 76-77 22.678148525527515 26.92233422695302 26.862465005391396 23.537052242128066 78-79 22.962773300745436 26.948986499120764 27.204516030352494 22.88372416978131 80-81 23.48056885317528 27.09623519056755 26.764526858025743 22.658669098231414 82-83 23.17726585029663 26.815432646701836 27.473814514913077 22.53348698808846 84-85 22.705536250431845 27.398105947732514 27.040403836542126 22.855953965293516 86-87 22.697280095420947 27.48983590027102 26.73153776640508 23.081346237902956 88-89 22.427284398248226 27.54947170349771 26.070965828006237 23.95227807024783 90-91 22.869985773222112 26.67525801248212 26.70165740280265 23.753098811493114 92-93 23.467219233635834 27.020876972531322 27.01498091993853 22.496922873894317 94-95 22.87647318260074 26.668268057845573 27.180897909531552 23.27436085002213 96-97 21.947576748371716 26.766219714796108 28.049494755221232 23.236708781610943 98-99 22.367688937221697 27.381096542674015 26.477666126879956 23.77354839322433 100-101 21.863709495663517 26.6854962523861 27.04760367006751 24.403190581882875 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 420.0 1 317.0 2 323.0 3 404.5 4 340.5 5 330.5 6 517.0 7 949.0 8 1512.5 9 1902.5 10 1847.0 11 1681.5 12 1726.5 13 1909.0 14 2187.5 15 2456.5 16 2677.5 17 2949.5 18 3278.0 19 3639.5 20 4054.0 21 4590.5 22 5441.0 23 6536.5 24 7689.5 25 9512.5 26 12498.0 27 15904.5 28 18399.0 29 21511.0 30 25856.5 31 30910.0 32 37830.0 33 43183.0 34 47890.0 35 52669.0 36 58819.0 37 64971.5 38 66215.0 39 65998.0 40 66345.0 41 69110.0 42 72488.5 43 75461.5 44 78491.5 45 81933.0 46 84630.5 47 103558.5 48 146554.5 49 143732.5 50 103265.0 51 85761.0 52 80097.0 53 79426.5 54 79227.5 55 74433.5 56 67562.0 57 61583.5 58 57242.0 59 52042.5 60 45283.0 61 38779.5 62 33154.5 63 26355.0 64 19917.0 65 15913.0 66 11967.0 67 9481.0 68 7162.5 69 4838.5 70 3821.0 71 3212.0 72 2838.0 73 2558.5 74 2126.0 75 1714.0 76 1188.0 77 741.0 78 496.5 79 360.0 80 252.0 81 165.5 82 111.5 83 78.5 84 47.5 85 25.0 86 14.0 87 7.0 88 4.5 89 3.0 90 3.0 91 1.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.980649358203516 2 0.011514706179123719 3 0.004392207511624511 4 0.012978775349665222 5 0.0 6 0.0 7 0.0 8 0.0 9 0.004590054696832823 10-11 2.1763190372914244E-4 12-13 1.9784718520831133E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0014047150149790104 24-25 0.0 26-27 0.0 28-29 0.0 30-31 1.978471852083113E-5 32-33 0.004530700541270329 34-35 5.935415556249339E-4 36-37 0.0035018951781871106 38-39 2.374166222499736E-4 40-41 0.0035018951781871106 42-43 0.010268268912311357 44-45 0.0027698605929163587 46-47 0.0017608399483539708 48-49 0.009318602423311463 50-51 4.748332444999472E-4 52-53 0.0024533050965830604 54-55 0.011099227090186264 56-57 0.0051835962524577565 58-59 0.0018399788224372953 60-61 0.006746589015603416 62-63 0.0037590965189579152 64-65 0.019191176965206197 66-67 0.0046098394153536535 68-69 5.144026815416095E-4 70-71 0.0020378260076456063 72-73 0.0 74-75 0.0 76-77 0.0011079442371665434 78-79 0.011613629771727875 80-81 0.017212705113123086 82-83 0.012899636475581898 84-85 0.009496664889998943 86-87 0.011653199208769538 88-89 0.029024182070059273 90-91 0.024908960617726395 92-93 0.0035018951781871106 94-95 0.009239463549228139 96-97 0.01222695604587364 98-99 0.011039872934623772 100-101 0.012998560068186053 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2527203.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.5212547971676 #Duplication Level Percentage of deduplicated Percentage of total 1 73.80394436087438 33.596481562873436 2 14.464783029391725 13.169101477333731 3 4.866001703889591 6.6451951021862925 4 2.2114215181399994 4.026667295647604 5 1.221868915094456 2.781050310637674 6 0.7198513725575962 1.9661122647771094 7 0.46802377948630036 1.4913520801990483 8 0.32329177855858465 1.1773317940475854 9 0.25073426520284287 1.0272364535411285 >10 1.407303215569277 12.112441907148922 >50 0.15277127309595023 4.851385168827543 >100 0.10065475372081191 8.3465931229793 >500 0.005417776952875734 1.6846806232665328 >1k 0.002796271975677651 2.3399049155418066 >5k 8.738349923992659E-4 2.7088464715674125 >10k+ 2.621504977197798E-4 2.0756194494249196 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 26849 1.06239981513159 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 14749 0.5836096269274768 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10582 0.4187237827748701 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 9553 0.3780068320589996 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8403 0.332501979461088 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 7540 0.2983535552941335 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7326 0.2898856957672178 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6678 0.2642447005642206 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6625 0.2621475204010125 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6345 0.2510680780293471 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5367 0.21236916860260138 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5145 0.20358475357935235 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5117 0.20247680934218582 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4328 0.17125652351631426 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4317 0.170821259708856 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3250 0.12860067038540235 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3233 0.1279279899556941 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3144 0.12440631005898617 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2770 0.10960734060540447 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.9784718520831133E-5 3.9569437041662266E-5 16-17 0.0 0.0 0.0 3.9569437041662266E-5 3.9569437041662266E-5 18-19 0.0 0.0 0.0 3.9569437041662266E-5 3.9569437041662266E-5 20-21 0.0 0.0 0.0 7.913887408332453E-5 3.9569437041662266E-5 22-23 0.0 0.0 0.0 9.892359260415566E-5 3.9569437041662266E-5 24-25 0.0 0.0 0.0 1.3849302964581792E-4 3.9569437041662266E-5 26-27 0.0 0.0 0.0 1.9784718520831133E-4 3.9569437041662266E-5 28-29 0.0 0.0 0.0 3.9569437041662265E-4 3.9569437041662266E-5 30-31 0.0 0.0 0.0 6.924651482290896E-4 3.9569437041662266E-5 32-33 0.0 0.0 0.0 0.001365145577937348 3.9569437041662266E-5 34-35 0.0 0.0 0.0 0.001978471852083113 3.9569437041662266E-5 36-37 0.0 0.0 0.0 0.0030666313707288255 3.9569437041662266E-5 38-39 0.0 0.0 0.0 0.003937158985645396 3.9569437041662266E-5 40-41 0.0 0.0 0.0 0.005697998933999367 3.9569437041662266E-5 42-43 0.0 0.0 0.0 0.007834748534249128 3.9569437041662266E-5 44-45 0.0 0.0 0.0 0.013018344786706885 3.9569437041662266E-5 46-47 0.0 0.0 0.0 0.02449348152878894 3.9569437041662266E-5 48-49 0.0 0.0 0.0 0.03213038287782976 3.9569437041662266E-5 50-51 0.0 0.0 0.0 0.04208209629380782 3.9569437041662266E-5 52-53 0.0 0.0 0.0 0.05567419791761881 3.9569437041662266E-5 54-55 0.0 0.0 0.0 0.07352001402340849 3.9569437041662266E-5 56-57 0.0 0.0 0.0 0.09245398964784388 3.9569437041662266E-5 58-59 0.0 0.0 0.0 0.12406996984413203 3.9569437041662266E-5 60-61 0.0 0.0 0.0 0.16468799696739833 3.9569437041662266E-5 62-63 0.0 0.0 0.0 0.21286378656562216 3.9569437041662266E-5 64-65 0.0 0.0 0.0 0.24916874505134728 3.9569437041662266E-5 66-67 0.0 0.0 0.0 0.31087728211781956 3.9569437041662266E-5 68-69 0.0 0.0 0.0 0.38679124708224866 3.9569437041662266E-5 70-71 0.0 0.0 0.0 0.4388646262290762 3.9569437041662266E-5 72-73 0.0 0.0 0.0 0.5252447072910249 7.913887408332453E-5 74-75 0.0 0.0 0.0 0.5948671317658296 7.913887408332453E-5 76-77 0.0 0.0 0.0 0.6578418908176351 7.913887408332453E-5 78-79 0.0 0.0 0.0 0.7429953193312924 7.913887408332453E-5 80-81 0.0 0.0 0.0 0.8322441845787616 7.913887408332453E-5 82-83 0.0 0.0 0.0 0.9495082112517277 7.913887408332453E-5 84-85 0.0 0.0 0.0 1.1083597162554808 7.913887408332453E-5 86-87 0.0 0.0 0.0 1.3270204253477065 7.913887408332453E-5 88-89 0.0 0.0 0.0 1.6057475398691756 7.913887408332453E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12550 0.0 23.35495 1 TACATGG 13110 0.0 21.810911 2 ACATGGG 13075 0.0 21.177393 3 GAGTACT 9605 0.0 20.912344 12-13 AGTACTT 10065 0.0 19.815054 12-13 GTACTTT 10175 0.0 19.46083 14-15 ATACCGT 270 2.30757E-8 19.347801 6 CATGGGG 5585 0.0 19.218245 4 AGAGTAC 17790 0.0 18.564768 10-11 TAGCCCG 130 0.007574712 18.265406 5 CATGGGA 6865 0.0 17.433657 4 TACTTTT 11370 0.0 17.311064 14-15 GATATAG 1005 0.0 17.183748 1 ACTTTTT 11805 0.0 16.975195 16-17 GAGTACA 11260 0.0 16.615282 1 GTACTAG 350 2.2178938E-8 16.447302 1 GTGTAAG 1170 0.0 16.400444 1 CGCGGAA 480 0.0 16.328907 82-83 GCACCGT 820 0.0 16.216116 6 AGTACAT 10300 0.0 16.186386 2 >>END_MODULE