FastQCFastQC Report
Thu 26 May 2016
SRR936745_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936745_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2537965
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT267571.0542698579373633No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT150480.5929159779587189No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT103120.4063097796856931No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC96130.37876802871592No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82950.3268366585039589No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT78880.3108001883398707No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66590.26237556467484774No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC65040.25626830945265205No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC62120.24476302864696714No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT61670.24298995455020064No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG54510.21477837558831586No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA49610.19547156875685834No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC48880.19259524855543714No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA41830.16481708770609524No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39620.15610932380864195No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT28990.11422537347835766No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA28880.11379195536581474No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC28370.11178247138947936No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC27950.1101276022324973No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG130800.026.5909581
TACATGG136250.024.970332
ACATGGG134100.024.2722243
GAGTACT87050.022.24477612-13
GTAAGCG3300.020.87512494-95
AGTACTT91250.020.70014612-13
CATGGGG60800.020.6335434
TAAGGTG10600.020.6209345
GTACTTT93900.020.31818214-15
CATGGGA69650.019.6491974
GTATAGG9750.019.4933321
ATGGGTA11000.019.43915
AGAGTAC169600.019.12274710-11
CATGGGT22300.018.5390664
ACTTTTT111900.017.77000216-17
ATTCGCG4150.017.74219180-81
TACTTTT108300.017.19976414-15
ATCGGTC1952.2711951E-417.0566648
GAGTACA113450.016.5433431
CGCGGAA4350.016.37802382-83
Produced by FastQC (version 0.11.5)