##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936745_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2537965 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.22961900577825 31.0 31.0 34.0 28.0 34.0 2 31.237411469425307 31.0 31.0 34.0 28.0 34.0 3 31.292904354472974 31.0 31.0 34.0 28.0 34.0 4 34.91072059701375 37.0 35.0 37.0 32.0 37.0 5 34.89424007029254 37.0 35.0 37.0 32.0 37.0 6 35.03463995760383 37.0 35.0 37.0 32.0 37.0 7 34.88856741523228 37.0 35.0 37.0 32.0 37.0 8 34.8255326610099 37.0 35.0 37.0 32.0 37.0 9 36.256297860687596 39.0 35.0 39.0 32.0 39.0 10-11 36.1567901842618 39.0 35.0 39.0 31.0 39.0 12-13 36.050734742204874 39.0 35.0 39.0 30.0 39.0 14-15 37.04327561648802 39.0 36.0 41.0 31.0 41.0 16-17 36.93726410726704 39.0 36.0 41.0 31.0 41.0 18-19 36.89154125450902 39.0 36.0 41.0 30.5 41.0 20-21 36.78686861323935 39.0 36.0 41.0 30.0 41.0 22-23 36.651485146564276 39.0 36.0 40.5 30.0 41.0 24-25 36.770955470229104 39.0 36.0 41.0 30.0 41.0 26-27 36.74636746369631 39.0 36.0 41.0 30.0 41.0 28-29 36.66088598542533 39.0 36.0 41.0 30.0 41.0 30-31 36.48678783986383 39.0 35.5 41.0 30.0 41.0 32-33 36.29540517698234 39.0 35.0 40.5 30.0 41.0 34-35 36.04338535007378 38.5 35.0 40.0 29.0 41.0 36-37 35.83027819532578 38.0 35.0 40.0 28.0 41.0 38-39 35.61415129838276 38.0 35.0 40.0 27.0 41.0 40-41 35.457060676565675 38.0 34.5 40.0 27.0 41.0 42-43 35.19715421607469 38.0 34.0 40.0 26.0 41.0 44-45 34.90841008445743 38.0 34.0 40.0 25.5 41.0 46-47 34.583183968258034 38.0 33.0 40.0 24.0 41.0 48-49 34.44301911176868 38.0 33.0 40.0 24.0 41.0 50-51 33.494762930142855 37.0 32.0 39.5 22.5 40.5 52-53 33.65193235525312 37.5 33.0 39.5 23.0 40.5 54-55 34.06747394073598 38.0 33.0 40.0 23.0 41.0 56-57 33.93552216047109 38.0 33.0 40.0 22.5 41.0 58-59 33.6639173511061 37.5 33.0 40.0 21.5 41.0 60-61 33.284048440384325 37.0 32.0 40.0 20.0 41.0 62-63 32.97977986300047 36.0 32.0 39.5 19.5 41.0 64-65 32.64887143833741 36.0 31.5 39.0 18.5 41.0 66-67 32.218973271893034 35.5 31.0 39.0 17.0 40.5 68-69 31.78555043115252 35.0 31.0 38.5 13.5 40.0 70-71 31.30830547308572 35.0 30.5 37.5 10.5 40.0 72-73 30.908323400834924 35.0 30.0 37.0 8.0 39.0 74-75 30.499917453550385 34.5 30.0 36.5 7.0 39.0 76-77 30.089970507867527 34.0 29.0 36.0 4.5 39.0 78-79 29.627484421573975 34.0 29.0 35.5 2.0 37.5 80-81 29.114192276095217 34.0 29.0 35.0 2.0 37.0 82-83 28.620343464153365 34.0 27.5 35.0 2.0 36.5 84-85 28.295982805121426 34.0 27.0 35.0 2.0 36.0 86-87 28.02745802247076 34.0 27.0 35.0 2.0 36.0 88-89 27.59877874596379 33.0 26.0 35.0 2.0 35.5 90-91 27.163466596269057 33.0 25.5 35.0 2.0 35.0 92-93 26.774483099648734 33.0 25.0 35.0 2.0 35.0 94-95 26.277117493740064 33.0 24.5 35.0 2.0 35.0 96-97 25.654035221131892 32.5 21.5 35.0 2.0 35.0 98-99 24.952089961839505 32.0 17.0 35.0 2.0 35.0 100-101 22.487117828654057 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4230791397188476 1101 2 0.4636704300088752 1101 3 0.4887082909767564 1101 4 0.5554076984179801 1101 5 0.5141919115198306 1101 6 0.36580367735474084 1101 7 0.4689495765298801 1101 8 0.5472514129957773 1101 9 0.7963981032469221 1101 10-11 0.593124967307368 1101 12-13 0.70922992908514 1101 14-15 0.9123232279110667 1101 16-17 0.9439905550474705 1101 18-19 0.9306626289067381 1101 20-21 0.914321147182207 1101 22-23 0.826650262231702 1101 24-25 0.861542426280387 1101 26-27 0.7058533295198046 1101 28-29 0.651369299554446 1101 30-31 0.7091336135603399 1101 32-33 0.5617939663103044 1101 34-35 0.5625837569706889 1101 36-37 0.6176103939977651 1101 38-39 0.46013106341571586 1101 40-41 0.5394038297028558 1101 42-43 0.572260987497458 1101 44-45 0.5689981176628933 1101 46-47 0.53787720680657 1101 48-49 0.7090698110703073 1101 50-51 0.5890648649044508 1101 52-53 0.6522784525401946 1101 54-55 0.7720490986226807 1101 56-57 0.6122813966872087 1101 58-59 0.6254261694488576 1101 60-61 0.6435736971834132 1101 62-63 0.6082119796236896 1101 64-65 0.6332121130424895 1101 66-67 0.5945993985523117 1101 68-69 0.5316653701732363 1101 70-71 0.6601120835090519 1101 72-73 0.598709553205385 1101 74-75 0.6183679753659064 1101 76-77 0.6329753015064021 1101 78-79 0.5819619109321756 1101 80-81 0.8234012533768293 1101 82-83 0.9437505148460836 1101 84-85 0.8481798616165825 1101 86-87 0.6790746021919425 1101 88-89 0.5777267323685749 1101 90-91 0.3670051980136755 1101 92-93 0.3578490588952299 1101 94-95 0.5661792905734906 1101 96-97 0.37504731153057946 1101 98-99 0.38642905991398635 1101 100-101 0.8360170719164621 1102 1 0.19637685908820757 1102 2 0.2725959948329759 1102 3 0.1649853801325598 1102 4 0.2086168380079343 1102 5 0.27180759645572294 1102 6 0.23731008829494016 1102 7 0.24089265676797567 1102 8 0.2492072511115424 1102 9 0.4662612654085123 1102 10-11 0.5267534527894142 1102 12-13 0.364343277921364 1102 14-15 0.3367212395595658 1102 16-17 0.2987670788458203 1102 18-19 0.3738829049718433 1102 20-21 0.44445679030298635 1102 22-23 0.3384205350966809 1102 24-25 0.2101434545909271 1102 26-27 0.2267905001724131 1102 28-29 0.28781911074639055 1102 30-31 0.1437713794383484 1102 32-33 0.12479014748069517 1102 34-35 -0.11300918566153229 1102 36-37 0.04151029372681592 1102 38-39 0.03091219541069279 1102 40-41 0.10801859726627328 1102 42-43 0.16564097171011838 1102 44-45 -0.22534489430561422 1102 46-47 -0.15776284183550615 1102 48-49 -0.11928416669940844 1102 50-51 0.008363224301128014 1102 52-53 0.13538796121256524 1102 54-55 0.2594046337921796 1102 56-57 0.17974701655365521 1102 58-59 0.2791269353482022 1102 60-61 0.09510664792264123 1102 62-63 0.3059455149211985 1102 64-65 0.2901364831489417 1102 66-67 0.1557674057206171 1102 68-69 0.1321288352810761 1102 70-71 -0.023370433078323316 1102 72-73 -0.024422228417300573 1102 74-75 -0.21696102000456108 1102 76-77 -0.13961761979157572 1102 78-79 -0.1882794646998498 1102 80-81 0.13382362873151266 1102 82-83 0.1616111168984382 1102 84-85 0.04812976881262543 1102 86-87 -0.0720167297048846 1102 88-89 0.06862912820413314 1102 90-91 0.06271018809325213 1102 92-93 -0.06631308135608904 1102 94-95 0.0729131458846517 1102 96-97 0.03379183396353724 1102 98-99 0.0719539463091401 1102 100-101 0.08445476525804096 1103 1 0.32720268210775316 1103 2 0.3926234712947263 1103 3 0.319363611913829 1103 4 0.40087634008202144 1103 5 0.4037559413548948 1103 6 0.30980619862798875 1103 7 0.28807595535118935 1103 8 0.2909564240263478 1103 9 0.5621180318108472 1103 10-11 0.41782335999567266 1103 12-13 0.3985443528691519 1103 14-15 0.3988054214187926 1103 16-17 0.5383513072273232 1103 18-19 0.3814444337181655 1103 20-21 0.572976888331489 1103 22-23 0.49095575650631673 1103 24-25 0.6541391276145632 1103 26-27 0.5887994373042389 1103 28-29 0.25424102977821406 1103 30-31 0.30015451911764757 1103 32-33 0.5491518820577568 1103 34-35 0.27220619630254106 1103 36-37 0.38528346890058174 1103 38-39 0.4289378708534741 1103 40-41 0.36708951097190123 1103 42-43 0.4354819140653703 1103 44-45 0.3879265765346247 1103 46-47 0.49529836449119813 1103 48-49 0.38920343855218675 1103 50-51 0.4258876404060814 1103 52-53 0.46776075110096826 1103 54-55 0.41648107677164603 1103 56-57 0.4871052227193573 1103 58-59 0.39419893969783004 1103 60-61 0.4465899804064364 1103 62-63 0.28820903813824117 1103 64-65 0.36547894779403123 1103 66-67 0.32632373934441006 1103 68-69 0.42064236355489015 1103 70-71 0.4892508585904629 1103 72-73 0.49822967086479863 1103 74-75 0.450857942799459 1103 76-77 0.5524265043638437 1103 78-79 0.4044251948905746 1103 80-81 0.45101801984389667 1103 82-83 0.4230487604601194 1103 84-85 0.6062590716176324 1103 86-87 0.6156948354097196 1103 88-89 0.6852174351734917 1103 90-91 0.6819689547108077 1103 92-93 0.5919328912305595 1103 94-95 0.5310608957841225 1103 96-97 0.6957318372160515 1103 98-99 0.41622462667519855 1103 100-101 0.9849255707083522 1104 1 0.4138766963301883 1104 2 0.3957684123621803 1104 3 0.4682906113732912 1104 4 0.5112239917891586 1104 5 0.6259839355423722 1104 6 0.5174303407306482 1104 7 0.6548687472585826 1104 8 0.6079978394370329 1104 9 0.7440748684306939 1104 10-11 0.7021608982836938 1104 12-13 0.5045911104676719 1104 14-15 0.7622567134420137 1104 16-17 0.636631183582459 1104 18-19 0.849712586743884 1104 20-21 0.9694947552939297 1104 22-23 0.6848270994008274 1104 24-25 0.7505605871573735 1104 26-27 0.32203308723766355 1104 28-29 0.7556066455572079 1104 30-31 0.8335973637917249 1104 32-33 0.710611144072999 1104 34-35 0.5960438667997678 1104 36-37 0.7063255582272063 1104 38-39 0.7624890568753386 1104 40-41 0.9366469962358437 1104 42-43 0.9117439269680574 1104 44-45 0.9008510207294691 1104 46-47 0.9969283986783921 1104 48-49 0.8932829699934075 1104 50-51 0.6759418110799729 1104 52-53 0.9202199592965385 1104 54-55 1.1050355036739958 1104 56-57 0.9826899032995797 1104 58-59 0.9624170170250039 1104 60-61 1.0845129621100895 1104 62-63 1.2587071977393194 1104 64-65 1.0121745366865866 1104 66-67 1.1191413865821787 1104 68-69 0.9934421333002774 1104 70-71 1.0699195329650486 1104 72-73 1.022495324785563 1104 74-75 0.8239118350150321 1104 76-77 1.135916499754071 1104 78-79 1.0697028459191174 1104 80-81 1.113992890735176 1104 82-83 1.1658779906281538 1104 84-85 1.114710866737827 1104 86-87 1.1517652502658215 1104 88-89 1.1801544076529176 1104 90-91 1.109329707999077 1104 92-93 1.159015139395425 1104 94-95 1.2503234258236908 1104 96-97 1.3853034559949045 1104 98-99 1.642341443451965 1104 100-101 2.0170610652526726 1105 1 0.3679982272760789 1105 2 0.39783014801057703 1105 3 0.2652243154490215 1105 4 0.26668698207747354 1105 5 0.4845472455824691 1105 6 0.3243604619258704 1105 7 0.45853411524387155 1105 8 0.5054286343401344 1105 9 0.5618497462111449 1105 10-11 0.5024335852726054 1105 12-13 0.4758384943782801 1105 14-15 0.25342067128856627 1105 16-17 0.46948303914454215 1105 18-19 0.4492582287608755 1105 20-21 0.4599597355700382 1105 22-23 0.27974884008511225 1105 24-25 0.46925370216061424 1105 26-27 0.41196880296090654 1105 28-29 0.19813661044136666 1105 30-31 0.3121185817544969 1105 32-33 0.23129844343845463 1105 34-35 0.14834422048512863 1105 36-37 0.21280994020739286 1105 38-39 0.2503129036977185 1105 40-41 0.051759893071441354 1105 42-43 0.28894588681271927 1105 44-45 0.07622918609609997 1105 46-47 0.2538371760136471 1105 48-49 0.200995097728061 1105 50-51 0.3771866589970756 1105 52-53 0.11563382711982939 1105 54-55 0.2502064666385664 1105 56-57 0.45266071681200515 1105 58-59 0.41050789223156414 1105 60-61 0.10798105507004863 1105 62-63 0.2803001446089013 1105 64-65 0.4848916235971643 1105 66-67 0.45662549149830056 1105 68-69 0.49833317131581367 1105 70-71 0.6059723809038076 1105 72-73 0.5377038403088221 1105 74-75 0.5569757303191096 1105 76-77 0.1982155267809027 1105 78-79 0.2893753868853395 1105 80-81 0.23026345507044255 1105 82-83 0.29479625853556257 1105 84-85 0.5945614506932024 1105 86-87 0.506859619006498 1105 88-89 0.10376279249579667 1105 90-91 0.26240692403774446 1105 92-93 0.2981292744456532 1105 94-95 0.3655570305354061 1105 96-97 0.44679091997431897 1105 98-99 0.40142950560095514 1105 100-101 0.9143643836137194 1106 1 0.127771151173377 1106 2 0.2216670726473673 1106 3 0.23701124347996583 1106 4 0.2424352485315353 1106 5 0.23777046390454615 1106 6 0.22223499956270132 1106 7 0.26939511815129435 1106 8 0.38781860959892356 1106 9 0.599824981888176 1106 10-11 0.29174568420279456 1106 12-13 0.49779238588379826 1106 14-15 0.7063483093764589 1106 16-17 0.6424560433460798 1106 18-19 0.6414591135517398 1106 20-21 0.5117391386900607 1106 22-23 0.43002658295847596 1106 24-25 0.4233893700939504 1106 26-27 0.12845745709953604 1106 28-29 0.45323441261853503 1106 30-31 0.46744545319695874 1106 32-33 0.3404233728472832 1106 34-35 0.3895579842838046 1106 36-37 0.3033258082369201 1106 38-39 0.3299815620826507 1106 40-41 0.286675479747295 1106 42-43 0.03832201248017242 1106 44-45 0.5287631023572814 1106 46-47 0.48861641881407536 1106 48-49 0.43423382805460164 1106 50-51 0.3112755887120997 1106 52-53 0.5768768528561807 1106 54-55 0.7410709214724918 1106 56-57 0.5916024196950147 1106 58-59 0.6329885275015812 1106 60-61 0.7026332409772351 1106 62-63 0.7728644927075194 1106 64-65 0.5912731380252083 1106 66-67 0.7347961004383592 1106 68-69 0.7569715812920208 1106 70-71 0.728254277643547 1106 72-73 0.772577036171711 1106 74-75 0.6369199650963324 1106 76-77 0.6536574383682563 1106 78-79 0.6580578919729554 1106 80-81 0.7308834297370552 1106 82-83 0.8584458836621209 1106 84-85 0.7648825376311983 1106 86-87 0.8516038803592174 1106 88-89 0.9693463630588077 1106 90-91 1.2022495941823372 1106 92-93 1.3967008412592037 1106 94-95 1.3300939740927973 1106 96-97 1.4552357187587788 1106 98-99 1.6714485128615522 1106 100-101 2.0360447207759442 1107 1 0.1729522329513813 1107 2 0.1922440624128896 1107 3 0.08167304336081571 1107 4 0.17511888670586728 1107 5 0.3243091477383402 1107 6 0.30894084948667455 1107 7 0.3510255210393538 1107 8 0.3452463858453285 1107 9 0.31415591739585835 1107 10-11 0.42015591690154963 1107 12-13 0.5089596142166712 1107 14-15 0.24223922461080605 1107 16-17 0.3890998284131584 1107 18-19 0.4961739975580386 1107 20-21 0.6397599099659459 1107 22-23 0.7785821078866775 1107 24-25 0.6229332200712818 1107 26-27 0.3834355387797359 1107 28-29 0.514470552504541 1107 30-31 0.59283958282181 1107 32-33 0.36972189769545594 1107 34-35 0.6244681325103372 1107 36-37 0.5862739778571608 1107 38-39 0.6099554201515218 1107 40-41 0.5348797780498487 1107 42-43 0.7414092500605349 1107 44-45 0.5764436728560227 1107 46-47 0.44160451267605083 1107 48-49 0.5369236015801206 1107 50-51 0.4228731808090558 1107 52-53 0.6850253105711701 1107 54-55 0.5076394817989964 1107 56-57 0.6110968311903449 1107 58-59 0.64355200171088 1107 60-61 0.6959714944472637 1107 62-63 0.5373897143359727 1107 64-65 0.6581955925213521 1107 66-67 0.6318235672496613 1107 68-69 0.6763512883956935 1107 70-71 0.6755676162526818 1107 72-73 0.6314968586911185 1107 74-75 0.7421279001505283 1107 76-77 0.7164798777483163 1107 78-79 0.7222309977944299 1107 80-81 0.7626094196181725 1107 82-83 0.8680481528528752 1107 84-85 0.828349633476055 1107 86-87 0.6904602153316084 1107 88-89 0.6284831458229192 1107 90-91 0.6591002917450872 1107 92-93 0.7012205499633986 1107 94-95 0.7074296311533423 1107 96-97 0.9386604895722925 1107 98-99 1.2190063763366474 1107 100-101 1.5237980263075421 1108 1 -0.004280921082976619 1108 2 0.1068412451333316 1108 3 0.052420242335298184 1108 4 0.060239923384727945 1108 5 0.27231845531614596 1108 6 0.10016050805966614 1108 7 -0.046948580414827745 1108 8 0.1826791158268648 1108 9 0.16794968979451852 1108 10-11 0.015100646577742793 1108 12-13 0.2090338432025618 1108 14-15 0.20623316520919133 1108 16-17 0.24136657632811165 1108 18-19 0.21929724794530614 1108 20-21 0.020370548664253363 1108 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2316 78-79 -2.9613839570578513 2316 80-81 -2.5058045899166643 2316 82-83 -2.4109928508723293 2316 84-85 -2.421100816839182 2316 86-87 -2.9814460753318777 2316 88-89 -3.216326621467541 2316 90-91 -3.329086225764893 2316 92-93 -3.908470551551872 2316 94-95 -4.21183445561136 2316 96-97 -5.263279754474407 2316 98-99 -6.579764272021428 2316 100-101 -6.736638737594035 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5880.0 3 2603.0 4 2661.0 5 2993.0 6 3269.0 7 3878.0 8 4872.0 9 6105.0 10 7623.0 11 9626.0 12 11860.0 13 14091.0 14 15949.0 15 18930.0 16 22908.0 17 27627.0 18 23754.0 19 18541.0 20 19070.0 21 20701.0 22 22922.0 23 25873.0 24 29449.0 25 34096.0 26 39597.0 27 46306.0 28 54706.0 29 65584.0 30 78011.0 31 95226.0 32 117143.0 33 147279.0 34 190237.0 35 252718.0 36 335976.0 37 413840.0 38 302995.0 39 43058.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.4172304614321 18.350903759888663 14.141529598683233 22.090336179996005 2 15.577454741791252 20.639595489056646 39.17186394654339 24.611085822608707 3 20.97930814873667 24.26236214365435 29.275280757177356 25.48304895043162 4 12.62410380153326 16.155047903032578 37.74971054765527 33.4711377477789 5 13.511454986061947 38.05023375953695 33.53939811107944 14.89891314332166 6 31.479323576507618 36.01933809900369 17.10670407930765 15.394634245181047 7 28.950572923298406 32.91997917000923 20.234223075640383 17.89522483105198 8 26.768296504539563 32.46185629103649 19.390528432322828 21.37931877210112 9 26.22919995868964 17.756398178244737 21.162058801037805 34.85234306202782 10-11 25.748807039516464 25.76045625502754 26.564114148120073 21.92662255733592 12-13 27.615591629917567 23.513883846441004 26.746267044623593 22.124257479017835 14-15 23.497756928373466 25.194595973153305 26.617954112127173 24.689692986346056 16-17 22.25336505171385 28.54348731491452 26.806880357724673 22.39626727564696 18-19 22.045364098801826 27.748074382252046 28.586521572615258 21.620039946330866 20-21 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25.83127848321721 27.875805095397844 23.524290574325683 46-47 23.66830670229118 26.727622615548828 27.484433320869222 22.119637361290774 48-49 23.284736043071273 26.640064966254034 27.433387721425266 22.641811269249423 50-51 21.983773556357953 26.932874328790568 28.376870993823378 22.7064811210281 52-53 23.095688089065398 27.484120021332885 26.755870238198202 22.66432165140351 54-55 22.259069970879946 26.520646079037896 27.46243749433558 23.75784645574658 56-57 23.204598315374984 26.600389826674515 26.955694850678096 23.239317007272405 58-59 24.274633878765048 26.098383407863235 26.75904486137754 22.86793785199418 60-61 23.025730143812176 26.712870194685312 28.3163394984011 21.94506016310141 62-63 22.40850276754476 26.930947128014754 28.026478746036442 22.63407135840404 64-65 22.097027794134945 27.807157195129083 27.25340612660505 22.84240888413092 66-67 22.70910620175001 26.972729291329617 26.49134009743634 23.826824409484036 68-69 22.398303545144543 26.829015021317996 27.290566760471908 23.48211467306555 70-71 23.421597703375525 26.692920258261893 26.953814339638697 22.93166769872388 72-73 23.10392742376061 26.104545451858925 28.115136447877102 22.676390676503367 74-75 22.153155144607428 28.245604288003605 26.854950468325615 22.746290099063348 76-77 22.681960708209743 26.833398514268715 26.820887715084773 23.66375306243677 78-79 23.00059204064886 26.963048191714776 27.09642972891237 22.93993003872399 80-81 23.454908380818814 27.04632196289128 26.733756143098557 22.765013513191345 82-83 23.154149263141306 26.827409567341792 27.40139096855544 22.617050200961465 84-85 22.649958483069486 27.398986963250326 27.02716708722181 22.92388746645838 86-87 22.709765937380375 27.458713519513495 26.75108600933902 23.080434533767114 88-89 22.42475838238846 27.515071178044217 26.052027136986457 24.008143302580866 90-91 22.834496744019635 26.634654238389423 26.689877745832774 23.84097127175817 92-93 23.529265722498387 27.0666258923005 26.938430969812522 22.465677415388594 94-95 22.895975495598023 26.662171723510557 27.222213793656497 23.219638987234926 96-97 21.961006067496093 26.78387242003348 28.055317752967497 23.199803759502934 98-99 22.464069689904516 27.435099227086496 26.452562689858798 23.648268393150186 100-101 22.171662028679318 26.60505238429338 26.9855775673822 24.237708019645098 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 286.0 1 226.0 2 238.5 3 332.5 4 330.0 5 345.0 6 518.0 7 937.5 8 1527.5 9 1893.0 10 1821.0 11 1701.0 12 1802.0 13 2003.0 14 2203.5 15 2375.5 16 2589.5 17 2780.0 18 2975.5 19 3226.0 20 3583.0 21 4195.0 22 4982.5 23 5981.5 24 7287.5 25 9055.5 26 11695.5 27 14966.0 28 17652.5 29 20866.5 30 25231.0 31 30572.5 32 37706.0 33 43073.5 34 47714.0 35 52602.0 36 58477.5 37 64570.5 38 66371.0 39 66403.5 40 66766.5 41 69507.0 42 72768.5 43 75810.0 44 78779.0 45 82806.5 46 86504.0 47 104071.0 48 143311.0 49 142817.0 50 106539.0 51 88298.5 52 82147.5 53 80884.5 54 80055.5 55 75531.0 56 68678.5 57 62215.0 58 57707.5 59 52149.0 60 45296.0 61 39048.5 62 33698.5 63 27016.0 64 20521.5 65 16700.5 66 12678.5 67 9791.0 68 7429.0 69 5119.5 70 4069.0 71 3434.5 72 2869.5 73 2570.0 74 2118.5 75 1694.0 76 1271.5 77 766.0 78 475.0 79 326.0 80 232.0 81 179.5 82 119.0 83 76.0 84 47.0 85 23.0 86 17.0 87 17.0 88 15.0 89 11.5 90 9.5 91 8.5 92 7.0 93 3.0 94 1.5 95 1.5 96 0.5 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03317618643283103 2 0.022852955024990493 3 0.02131629080779286 4 0.05035530434816871 5 0.0545318788872187 6 0.04771539402631636 7 0.048779238484376264 8 0.04121412233817251 9 0.04219916350304279 10-11 0.051754062802284506 12-13 0.04602112322273948 14-15 0.04105651575179327 16-17 0.049862783765733575 18-19 0.051675259509094885 20-21 0.011899297271632981 22-23 0.021099581751521396 24-25 0.006166357692087953 26-27 0.010855153636870484 28-29 0.006422468394954225 30-31 0.009495796829349498 32-33 0.003959865482778525 34-35 0.008175841668423323 36-37 0.009062378716806575 38-39 0.011978100564822604 40-41 0.0022261930326068326 42-43 0.00197008232974056 44-45 0.0026399103218523504 46-47 4.728197591377344E-4 48-49 0.0012608526910339582 50-51 3.5461481935330076E-4 52-53 0.003920463836183714 54-55 0.007683321085988183 56-57 0.01682450309598438 58-59 0.011071862693141947 60-61 0.011032461046547136 62-63 0.006146656868790547 64-65 5.91024698922168E-5 66-67 0.0080970383752337 68-69 0.017257921208527306 70-71 0.006048152752303519 72-73 0.00214738973941721 74-75 0.003703754779912253 76-77 0.006205759338682763 78-79 0.0052207181738124836 80-81 0.010224727291353507 82-83 0.006501271688143847 84-85 0.017573134381285795 86-87 0.0046690951214851265 88-89 0.006855886507497149 90-91 0.0038022588963992805 92-93 0.016666896509605136 94-95 0.011761391508551142 96-97 0.01034293223113794 98-99 0.02178911056693059 100-101 0.03723455603209658 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2537965.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.55688888104913 #Duplication Level Percentage of deduplicated Percentage of total 1 75.68058453189064 35.991331490359684 2 13.413223365150026 12.757823462262635 3 4.591435429751219 6.550631536115725 4 2.064801517146175 3.927821452493692 5 1.0765014751687423 2.559753051744267 6 0.6664451432620941 1.9016434574058168 7 0.44751546904633344 1.4897710403790945 8 0.3007390869431199 1.14417722719537 9 0.21423457609836188 0.9169496936989614 >10 1.3056599791742305 11.81383510099297 >50 0.13704808279716654 4.546328643993036 >100 0.09390270349543046 8.146846174068425 >500 0.004661935202975538 1.5810094117626239 >1k 0.00233096760148773 2.3604287609229777 >5k 6.659907432822086E-4 2.2483082958027376 >10k+ 2.4974652873082823E-4 2.0633412008021144 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 26757 1.0542698579373633 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 15048 0.5929159779587189 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10312 0.4063097796856931 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 9613 0.37876802871592 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8295 0.3268366585039589 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 7888 0.3108001883398707 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6659 0.26237556467484774 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6504 0.25626830945265205 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6212 0.24476302864696714 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6167 0.24298995455020064 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5451 0.21477837558831586 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4961 0.19547156875685834 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4888 0.19259524855543714 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4183 0.16481708770609524 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3962 0.15610932380864195 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2899 0.11422537347835766 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2888 0.11379195536581474 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2837 0.11178247138947936 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2795 0.1101276022324973 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.94016465948112E-5 0.0 18-19 0.0 0.0 0.0 7.88032931896224E-5 0.0 20-21 0.0 0.0 0.0 9.8504116487028E-5 0.0 22-23 0.0 0.0 0.0 2.364098795688672E-4 0.0 24-25 0.0 0.0 0.0 3.94016465948112E-4 0.0 26-27 0.0 0.0 0.0 4.5311893584032875E-4 0.0 28-29 0.0 0.0 0.0 6.501271688143847E-4 0.0 30-31 0.0 0.0 0.0 0.0012805535143313638 0.0 32-33 0.0 0.0 0.0 0.002107988092822399 0.0 34-35 0.0 0.0 0.0 0.0029551234946108397 0.0 36-37 0.0 0.0 0.0 0.003861361366291497 0.0 38-39 0.0 0.0 0.0 0.004885804177756589 0.0 40-41 0.0 0.0 0.0 0.006698279921117904 0.0 42-43 0.0 0.0 0.0 0.009456395182754688 0.0 44-45 0.0 0.0 0.0 0.015169633939002312 0.0 46-47 0.0 0.0 0.0 0.027443246853286002 0.0 48-49 0.0 0.0 0.0 0.03713605191560955 0.0 50-51 0.0 0.0 0.0 0.04982338211913876 0.0 52-53 0.0 0.0 0.0 0.06483540947176182 1.97008232974056E-5 54-55 0.0 0.0 0.0 0.08416191712651672 3.94016465948112E-5 56-57 0.0 0.0 0.0 0.10719217956118386 3.94016465948112E-5 58-59 0.0 0.0 0.0 0.14054567340369153 3.94016465948112E-5 60-61 0.0 0.0 0.0 0.18451791100350085 3.94016465948112E-5 62-63 0.0 0.0 0.0 0.23601586310291905 3.94016465948112E-5 64-65 0.0 0.0 0.0 0.27478708335221325 3.94016465948112E-5 66-67 0.0 0.0 0.0 0.3392481771813244 3.94016465948112E-5 68-69 0.0 0.0 0.0 0.42108539715874727 3.94016465948112E-5 70-71 0.0 0.0 0.0 0.4783162888377105 3.94016465948112E-5 72-73 0.0 0.0 0.0 0.5647240998201315 7.88032931896224E-5 74-75 0.0 0.0 0.0 0.6384642814223207 7.88032931896224E-5 76-77 0.0 0.0 0.0 0.7036346048901383 7.88032931896224E-5 78-79 0.0 0.0 0.0 0.7938052731223638 7.88032931896224E-5 80-81 0.0 0.0 0.0 0.8860642286241143 7.88032931896224E-5 82-83 0.0 0.0 0.0 1.0052936112200128 7.88032931896224E-5 84-85 0.0 0.0 0.0 1.1735189413565592 7.88032931896224E-5 86-87 0.0 0.0 0.0 1.3989554623487717 7.88032931896224E-5 88-89 0.0 0.0 0.0 1.6828443260643862 7.88032931896224E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 13080 0.0 26.590958 1 TACATGG 13625 0.0 24.97033 2 ACATGGG 13410 0.0 24.272224 3 GAGTACT 8705 0.0 22.244776 12-13 GTAAGCG 330 0.0 20.875124 94-95 AGTACTT 9125 0.0 20.700146 12-13 CATGGGG 6080 0.0 20.633543 4 TAAGGTG 1060 0.0 20.620934 5 GTACTTT 9390 0.0 20.318182 14-15 CATGGGA 6965 0.0 19.649197 4 GTATAGG 975 0.0 19.493332 1 ATGGGTA 1100 0.0 19.4391 5 AGAGTAC 16960 0.0 19.122747 10-11 CATGGGT 2230 0.0 18.539066 4 ACTTTTT 11190 0.0 17.770002 16-17 ATTCGCG 415 0.0 17.742191 80-81 TACTTTT 10830 0.0 17.199764 14-15 ATCGGTC 195 2.2711951E-4 17.056664 8 GAGTACA 11345 0.0 16.543343 1 CGCGGAA 435 0.0 16.378023 82-83 >>END_MODULE