FastQCFastQC Report
Thu 26 May 2016
SRR936745_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936745_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2537965
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT275611.0859487817995914No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT151830.5982352002490184No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT109090.42983256270279535No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC97760.3851904971108743No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85090.33526861087524845No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT76670.30209242444241746No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75550.2976794400237986No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC68960.271713754917818No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC67670.2666309425070874No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT67290.2651336799364845No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG56230.2215554588026234No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA53890.21233547349943754No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC52070.2051643738191819No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA45230.17821364754833105No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44770.17640117180496975No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC33600.13238953255856561No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT32120.12655808886253359No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA32060.1263216789829647No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC29400.11584084098874492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG128900.025.2151281
TACATGG133100.023.731322
ACATGGG131800.022.7396453
CGTATAG1954.210233E-721.9313771
GAGTACT102300.021.66022112-13
GTACTTT107600.020.5491714-15
AGTACTT104900.020.44415312-13
GTAAGGT9350.019.8109654
CATGGGG58600.019.452094
GTATAGG9300.019.415951
TAAGGTG10750.018.9978335
ACCGTCC2251.671393E-618.997468
CATGGGA67400.018.885464
AGAGTAC182600.018.63812810-11
GTATTCG1300.007570559618.2671475
GTGTAGC13950.018.0534291
ACTTTTT123950.017.89481516-17
GGTAAGG9300.017.875363
TACTTTT121450.017.59956214-15
ATACCGT2755.9215745E-717.2704186
Produced by FastQC (version 0.11.5)