FastQCFastQC Report
Thu 26 May 2016
SRR936744_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936744_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2577188
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT265191.0289897360999662No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT152000.5897901123239748No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT103820.4028421675097044No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC94690.3674159587891919No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80430.31208433377774536No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT80040.3105710565158615No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66540.2581883820660347No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC65730.25504542159904514No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT64430.25000116405943223No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC60340.23413115380018842No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG54490.21143199487193018No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA49840.19338907367254543No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC48400.18780158839789726No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA41680.16172665711620574No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38840.15070689449120514No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT29400.1140778243574004No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC28840.11190491341725943No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA27660.10732627965053385No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC27110.10519217069146683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG135700.027.0392361
TACATGG138350.025.868092
ACATGGG136900.024.7909933
GTATAGG8900.024.5654531
GAGTACT89100.022.22047412-13
CATTCCG4500.022.1865759
GTAAGCG2250.022.16913294-95
CATGGGA67150.021.378074
GTACTTT96750.020.4102114-15
AGTACTT93350.020.21585812-13
CATGGGG61600.019.9860364
ATGGGAG29100.019.2765965
AGAGTAC171750.018.84969710-11
CGTACAC2905.178481E-818.029923
ACTTTTT112950.017.8377316-17
GTACTAA4550.017.7580171
ATGGGTA10850.017.5255475
CATGGGT22250.017.5182784
TACTTTT111050.017.03303114-15
GTACCTG21050.016.7084661
Produced by FastQC (version 0.11.5)