##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936744_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2577188 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.187431417498452 31.0 31.0 34.0 28.0 34.0 2 31.217752061549255 31.0 31.0 34.0 28.0 34.0 3 31.16267497753365 31.0 31.0 34.0 27.0 34.0 4 34.82049349911609 37.0 35.0 37.0 32.0 37.0 5 34.86497531417964 37.0 35.0 37.0 32.0 37.0 6 34.93510601477269 37.0 35.0 37.0 32.0 37.0 7 34.79090582448777 37.0 35.0 37.0 32.0 37.0 8 34.777756609141434 37.0 35.0 37.0 32.0 37.0 9 36.21725578421132 39.0 35.0 39.0 32.0 39.0 10-11 36.080149760126154 39.0 35.0 39.0 30.0 39.0 12-13 35.98279442555219 39.0 35.0 39.0 30.0 39.0 14-15 36.89863583875138 39.0 36.0 41.0 31.0 41.0 16-17 36.83357112480735 39.0 36.0 41.0 30.5 41.0 18-19 36.714502783654126 39.0 36.0 41.0 30.0 41.0 20-21 36.688304462072615 39.0 36.0 41.0 30.0 41.0 22-23 36.69096957614268 39.0 36.0 41.0 30.0 41.0 24-25 36.78554474877269 39.0 36.0 41.0 30.0 41.0 26-27 36.70258882937527 39.0 36.0 41.0 30.0 41.0 28-29 36.60788561020772 39.0 36.0 41.0 30.0 41.0 30-31 36.424680698497745 39.0 35.5 41.0 30.0 41.0 32-33 36.23772557531697 39.0 35.0 40.0 30.0 41.0 34-35 36.00800310260641 38.0 35.0 40.0 29.0 41.0 36-37 35.780919358618775 38.0 35.0 40.0 28.0 41.0 38-39 35.578196468398886 38.0 35.0 40.0 27.0 41.0 40-41 35.373498557342344 38.0 34.5 40.0 26.5 41.0 42-43 35.067910451236 38.0 34.0 40.0 25.5 41.0 44-45 34.889983190981795 38.0 34.0 40.0 25.0 41.0 46-47 34.702701549130296 38.0 34.0 40.0 24.0 41.0 48-49 34.43240694896919 38.0 33.0 40.0 23.0 41.0 50-51 33.42235063953425 37.0 32.0 39.5 22.5 40.5 52-53 33.539326389848156 37.5 33.0 39.5 22.5 40.5 54-55 33.976100307777315 38.0 33.0 40.0 22.5 41.0 56-57 33.78807095175051 38.0 33.0 40.0 22.0 41.0 58-59 33.54742281121905 37.0 33.0 40.0 20.5 41.0 60-61 33.195108389453935 37.0 32.0 40.0 20.0 41.0 62-63 32.87872440815339 36.0 32.0 39.5 18.5 41.0 64-65 32.52870997381642 36.0 31.5 39.0 18.0 41.0 66-67 32.12616425344213 35.5 31.0 39.0 15.5 40.5 68-69 31.668142176666972 35.0 31.0 38.5 12.5 40.0 70-71 31.23592768552391 35.0 30.0 37.5 9.5 40.0 72-73 30.818782525760636 35.0 30.0 37.0 7.5 39.0 74-75 30.419564463283237 34.5 30.0 36.5 7.0 39.0 76-77 29.980738308575084 34.0 29.0 36.0 2.0 39.0 78-79 29.496443410414763 34.0 29.0 35.5 2.0 37.5 80-81 29.0104307485523 34.0 29.0 35.0 2.0 37.0 82-83 28.603597021249517 34.0 27.5 35.0 2.0 36.5 84-85 28.22740483038102 34.0 27.0 35.0 2.0 36.0 86-87 27.928717268588862 34.0 27.0 35.0 2.0 36.0 88-89 27.55737338525556 33.0 26.0 35.0 2.0 35.5 90-91 27.169196620502657 33.0 25.5 35.0 2.0 35.0 92-93 26.731383779530248 33.0 25.0 35.0 2.0 35.0 94-95 26.213857312698956 33.0 24.5 35.0 2.0 35.0 96-97 25.57768525229824 32.0 21.5 35.0 2.0 35.0 98-99 24.850600344251177 32.0 10.5 35.0 2.0 35.0 100-101 22.291954642036202 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5793706414771016 1101 2 0.5432186009578821 1101 3 0.6972833341303755 1101 4 0.7002240473211643 1101 5 0.5057017069936052 1101 6 0.5579401731299569 1101 7 0.622563672885704 1101 8 0.5253917435278339 1101 9 0.737042796825385 1101 10-11 0.8525565780331377 1101 12-13 0.8084283150861395 1101 14-15 1.0388964488660406 1101 16-17 1.0579822547418658 1101 18-19 1.1660772610539638 1101 20-21 1.0333757146944862 1101 22-23 1.0111698303139605 1101 24-25 0.9533037324491715 1101 26-27 0.8512964459531105 1101 28-29 0.8798254216616002 1101 30-31 0.7178665312189949 1101 32-33 0.6395767390706695 1101 34-35 0.6997171550311236 1101 36-37 0.7063757605156837 1101 38-39 0.6803214309451064 1101 40-41 0.6046320080774876 1101 42-43 0.6170298031433532 1101 44-45 0.5546917279267873 1101 46-47 0.7448979359371464 1101 48-49 0.7577201810239842 1101 50-51 0.7361097773458951 1101 52-53 0.7841185055119837 1101 54-55 0.7017228166166305 1101 56-57 0.9591126830937 1101 58-59 0.7553138525461591 1101 60-61 0.6852124148871752 1101 62-63 0.6673001318367611 1101 64-65 0.6012271227978943 1101 66-67 0.5810715992609943 1101 68-69 0.6485804682434839 1101 70-71 0.49320916889572075 1101 72-73 0.5448831613273235 1101 74-75 0.4837795720382623 1101 76-77 0.5694361849671417 1101 78-79 0.7348589741565767 1101 80-81 0.6990450263567638 1101 82-83 0.7704055620738508 1101 84-85 0.6101361692180376 1101 86-87 0.7599228268304081 1101 88-89 0.7292717611219928 1101 90-91 0.505449133795377 1101 92-93 0.5441632852643501 1101 94-95 0.4823492136801093 1101 96-97 0.3626900997329692 1101 98-99 0.4708925708437697 1101 100-101 0.8241254849866735 1102 1 0.3902842834471194 1102 2 0.510026196612607 1102 3 0.5122296118242389 1102 4 0.4872347258249903 1102 5 0.3773545335981794 1102 6 0.33603835732260734 1102 7 0.5239804379388318 1102 8 0.4376641080348378 1102 9 0.5527017442771225 1102 10-11 0.5179569724584212 1102 12-13 0.48789099308868344 1102 14-15 0.6831334206296731 1102 16-17 0.5917616799890482 1102 18-19 0.6497819895403083 1102 20-21 0.7037893665581123 1102 22-23 0.2585064703118434 1102 24-25 0.6870869502471137 1102 26-27 0.5327116280285225 1102 28-29 0.34914582954218787 1102 30-31 0.2939767592337361 1102 32-33 0.49322698674072996 1102 34-35 0.14471840822181292 1102 36-37 0.181424568995169 1102 38-39 0.027694842196119396 1102 40-41 0.3804990181623751 1102 42-43 0.26536553292905296 1102 44-45 0.31324211608611563 1102 46-47 0.2767243624309188 1102 48-49 0.4086658453139833 1102 50-51 0.402327548909021 1102 52-53 0.2117660357369644 1102 54-55 0.393760768507299 1102 56-57 0.24284099135223158 1102 58-59 0.27286858070271336 1102 60-61 0.431366438618646 1102 62-63 0.3506063127051533 1102 64-65 0.575414527571894 1102 66-67 0.4473380144100929 1102 68-69 0.48066149031943084 1102 70-71 0.0723817992110618 1102 72-73 0.462551501113488 1102 74-75 0.37731986871466816 1102 76-77 0.5637664864584444 1102 78-79 0.5020291706436844 1102 80-81 0.5868993000668041 1102 82-83 0.47534989402427996 1102 84-85 0.5184139884024077 1102 86-87 0.41933659741736307 1102 88-89 0.4531112405861535 1102 90-91 0.6914945256812963 1102 92-93 0.4724370810470333 1102 94-95 0.6320431713222661 1102 96-97 0.499391932689246 1102 98-99 0.5310416015997106 1102 100-101 1.1028026102991468 1103 1 0.21752947025488822 1103 2 0.38559009149184575 1103 3 0.41209326451090433 1103 4 0.36111503326051064 1103 5 0.3966922051407735 1103 6 0.3636920829437642 1103 7 0.40020468650350693 1103 8 0.3481978184615713 1103 9 0.5019321806702806 1103 10-11 0.3928062703137769 1103 12-13 0.36305175776567467 1103 14-15 0.8590717674939725 1103 16-17 0.90603531923594 1103 18-19 0.8856392575501886 1103 20-21 0.6576847300576532 1103 22-23 0.5638409646098452 1103 24-25 0.5232112212638143 1103 26-27 0.6472716721024199 1103 28-29 0.5254986269576349 1103 30-31 0.5075535366700237 1103 32-33 0.6365951175687385 1103 34-35 0.6271844456028575 1103 36-37 0.5429424265310914 1103 38-39 0.4888416752970741 1103 40-41 0.7813692385381259 1103 42-43 0.6503652530648765 1103 44-45 0.6733642689397499 1103 46-47 0.8853231422230863 1103 48-49 0.8839175186816703 1103 50-51 0.8931594813491799 1103 52-53 0.9986153436548548 1103 54-55 1.0073294959176948 1103 56-57 1.0302166954936496 1103 58-59 0.9774356182365906 1103 60-61 1.0840831336805294 1103 62-63 0.9883233882565392 1103 64-65 0.9654491301050001 1103 66-67 1.116550501761175 1103 68-69 1.0035707319378098 1103 70-71 0.9232904458822162 1103 72-73 0.9822897872252447 1103 74-75 0.9094627415802314 1103 76-77 0.9606269449199942 1103 78-79 0.9265057774571517 1103 80-81 0.8000062830240182 1103 82-83 0.7147843922844039 1103 84-85 0.8860264387736834 1103 86-87 0.6907094161276355 1103 88-89 0.7514514887659374 1103 90-91 0.8759803648681945 1103 92-93 0.8943519872422208 1103 94-95 0.892133061181557 1103 96-97 0.9869310586451085 1103 98-99 1.2120802373215724 1103 100-101 1.7401830626980228 1104 1 0.295838116614739 1104 2 0.29731697189305706 1104 3 0.5247843060512665 1104 4 0.5134352739480335 1104 5 0.4410432588063955 1104 6 0.37976073772194496 1104 7 0.460952039933197 1104 8 0.4704255412930465 1104 9 0.5899159203910145 1104 10-11 0.6028722955615748 1104 12-13 0.6105264101211887 1104 14-15 0.6619021299004118 1104 16-17 0.6144220144579435 1104 18-19 0.6633530608507954 1104 20-21 0.6714514994422203 1104 22-23 0.631385157819949 1104 24-25 0.7160826110405765 1104 26-27 0.2666117245495343 1104 28-29 0.19263314435689693 1104 30-31 0.28841105773675935 1104 32-33 0.35818432130772493 1104 34-35 0.4374778492961653 1104 36-37 0.38427929579955133 1104 38-39 0.2895948103453989 1104 40-41 0.540650769285925 1104 42-43 0.5172245008446055 1104 44-45 0.510933968235797 1104 46-47 0.6927202060935329 1104 48-49 0.7653086885297213 1104 50-51 0.6881072315402079 1104 52-53 0.48590370472440725 1104 54-55 0.7273138072234815 1104 56-57 0.8431556231857513 1104 58-59 0.745434119918464 1104 60-61 0.7081867436347125 1104 62-63 0.808951958420991 1104 64-65 0.7356835995533046 1104 66-67 0.6460288347417489 1104 68-69 0.5310535993336636 1104 70-71 0.5353441342200931 1104 72-73 0.8033667766222194 1104 74-75 0.7323229447918713 1104 76-77 0.545143806081029 1104 78-79 0.4183300485367418 1104 80-81 0.7511102014906399 1104 82-83 0.5406597664155157 1104 84-85 0.6586246754696852 1104 86-87 0.6019493132423293 1104 88-89 0.6119975916440694 1104 90-91 0.7843584504409336 1104 92-93 0.5376827675753901 1104 94-95 0.6621439189059082 1104 96-97 0.6496036552077058 1104 98-99 0.4738632000214622 1104 100-101 0.8454086713822875 1105 1 0.5206674175366217 1105 2 0.5061706695350772 1105 3 0.5196955357512678 1105 4 0.49127744325846834 1105 5 0.56129698831149 1105 6 0.47976192411302065 1105 7 0.5818998082185871 1105 8 0.5921249730682163 1105 9 0.5928454589300856 1105 10-11 0.726261063488792 1105 12-13 0.588463914428921 1105 14-15 0.873654576408299 1105 16-17 0.8320442720584751 1105 18-19 0.7679995332581697 1105 20-21 0.7170241704153 1105 22-23 0.7859521420251241 1105 24-25 0.5640067656239438 1105 26-27 0.6889803656345919 1105 28-29 0.6261028750009245 1105 30-31 0.38994212366959147 1105 32-33 0.6072941042885489 1105 34-35 0.38547818713514204 1105 36-37 0.48694181160397676 1105 38-39 0.20863675435452222 1105 40-41 0.5047724670228675 1105 42-43 0.4587673402965109 1105 44-45 0.4495065928831252 1105 46-47 0.6121006952708257 1105 48-49 0.6512195318052036 1105 50-51 0.6370825286181798 1105 52-53 0.871276434904054 1105 54-55 0.6668985364494944 1105 56-57 0.6187568127405356 1105 58-59 0.6046398649890108 1105 60-61 0.3309044944180144 1105 62-63 0.6520148755645678 1105 64-65 0.6060991866982448 1105 66-67 0.6347336854994055 1105 68-69 0.7436205598110242 1105 70-71 0.983205353581539 1105 72-73 0.8607416377862194 1105 74-75 0.7861544512712619 1105 76-77 0.8044558461426305 1105 78-79 0.8212526099663755 1105 80-81 0.9366037175809296 1105 82-83 0.9199149557465844 1105 84-85 1.0919209670371544 1105 86-87 1.1567244243393802 1105 88-89 1.000088146050274 1105 90-91 0.9362882567654189 1105 92-93 1.147483685503115 1105 94-95 1.175293651628948 1105 96-97 1.286987035789533 1105 98-99 1.3387911634851406 1105 100-101 1.4380930732773507 1106 1 0.17834791037965303 1106 2 0.12718566549779808 1106 3 0.15241519530208691 1106 4 0.09389780145568949 1106 5 0.2751054478792909 1106 6 0.2247382063040817 1106 7 0.41893997673398786 1106 8 0.5024692569067213 1106 9 0.5544214230458522 1106 10-11 0.5995953240133574 1106 12-13 0.6251181085816597 1106 14-15 0.4613827640049095 1106 16-17 0.2219353054944193 1106 18-19 0.5067460828248969 1106 20-21 0.37423959737031964 1106 22-23 0.18329815132148042 1106 24-25 -0.03647882436327876 1106 26-27 0.05912443130289091 1106 28-29 0.13187402012987803 1106 30-31 0.14652885707932484 1106 32-33 0.22167619106426883 1106 34-35 0.20102008538137994 1106 36-37 0.03789905614234357 1106 38-39 0.31686643570306927 1106 40-41 0.38095811625291276 1106 42-43 0.3391716636725022 1106 44-45 0.09773807439779603 1106 46-47 0.3342391961376876 1106 48-49 0.31320705304261054 1106 50-51 0.08965552563012835 1106 52-53 0.19786020203699195 1106 54-55 0.4542452983421015 1106 56-57 0.47817755346616764 1106 58-59 0.4232847754672733 1106 60-61 0.5558749488124732 1106 62-63 0.4091211898471059 1106 64-65 0.157126453012296 1106 66-67 0.14268177730953369 1106 68-69 0.32905112343394194 1106 70-71 0.1720867502016965 1106 72-73 0.26719185630102515 1106 74-75 0.4871614691623769 1106 76-77 0.21366131125951782 1106 78-79 0.3339631473890172 1106 80-81 0.48880226349881184 1106 82-83 0.5865995183985682 1106 84-85 0.8791666935723512 1106 86-87 0.8590073409834176 1106 88-89 0.698848608998965 1106 90-91 0.8154982126489401 1106 92-93 0.9176356906483392 1106 94-95 1.0710540398693666 1106 96-97 1.1255256427366334 1106 98-99 1.1681821421993455 1106 100-101 1.7697831232657322 1107 1 0.2850148670093198 1107 2 0.24500351180114066 1107 3 0.3865158520682854 1107 4 0.3739618815109438 1107 5 0.46658936713237154 1107 6 0.3647471067537751 1107 7 0.4187819115368683 1107 8 0.4469012512121324 1107 9 0.45628786538016897 1107 10-11 0.4609873957176731 1107 12-13 0.4956944710453115 1107 14-15 0.3229527253585047 1107 16-17 0.3299106096638411 1107 18-19 0.5436220170273387 1107 20-21 0.3042787078982059 1107 22-23 0.32344131436519774 1107 24-25 0.34186905485794483 1107 26-27 0.24679953624651318 1107 28-29 0.27864140120173175 1107 30-31 0.13671436090948674 1107 32-33 0.20929452779406432 1107 34-35 0.269636227232688 1107 36-37 0.4649829972487325 1107 38-39 0.5007286155812594 1107 40-41 0.4742139304531108 1107 42-43 0.4115978132785614 1107 44-45 0.39678602331620993 1107 46-47 0.43361658899547706 1107 48-49 0.5182085302626618 1107 50-51 0.4197679269587269 1107 52-53 0.3814218989146241 1107 54-55 0.5018699311587724 1107 56-57 0.5231936464127429 1107 58-59 0.7063578625866569 1107 60-61 0.7610714217308114 1107 62-63 0.638800463362486 1107 64-65 0.7624963335298531 1107 66-67 0.7323479733167417 1107 68-69 0.6572495700455612 1107 70-71 0.6578084371710773 1107 72-73 0.6727434587532599 1107 74-75 0.4769738224349105 1107 76-77 0.714054219966787 1107 78-79 0.7623486731785754 1107 80-81 0.7965668715342531 1107 82-83 0.6828158725240172 1107 84-85 0.7827083352650703 1107 86-87 0.9117462183386138 1107 88-89 0.9012178763508629 1107 90-91 0.7809058696877749 1107 92-93 0.9225347835238402 1107 94-95 1.0876521453147667 1107 96-97 1.3213503213815976 1107 98-99 1.3196916780923438 1107 100-101 1.7407757727036426 1108 1 0.2203564037779593 1108 2 0.2039854337458955 1108 3 0.41632027607111155 1108 4 0.42174300268830933 1108 5 0.3566866966370199 1108 6 0.3564774731677005 1108 7 0.4389134014154834 1108 8 0.3477234750828728 1108 9 -0.04348237249060816 1108 10-11 0.1879224461880611 1108 12-13 0.18459918827819166 1108 14-15 0.7123963790296983 1108 16-17 0.7845575100902877 1108 18-19 0.6723186243782848 1108 20-21 0.7046351611745791 1108 22-23 0.7468055342197388 1108 24-25 0.687125548729064 1108 26-27 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-4.840622080624019 2316 94-95 -4.390251406065659 2316 96-97 -5.7747887766131605 2316 98-99 -7.307174316872668 2316 100-101 -8.03239972287008 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6668.0 3 2678.0 4 2926.0 5 3105.0 6 3415.0 7 4064.0 8 5101.0 9 6480.0 10 8201.0 11 10158.0 12 12668.0 13 14991.0 14 16653.0 15 19755.0 16 23439.0 17 28515.0 18 23701.0 19 19141.0 20 19661.0 21 21429.0 22 23651.0 23 26599.0 24 30778.0 25 35273.0 26 40554.0 27 47365.0 28 56041.0 29 67021.0 30 80307.0 31 96752.0 32 118520.0 33 148781.0 34 191547.0 35 254080.0 36 339547.0 37 417281.0 38 306886.0 39 43451.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.35638843154919 18.329307881084773 14.194495355844802 22.11980833152123 2 15.5994339419053 20.613651377457195 39.11925645490204 24.66765822573546 3 20.952321447005158 24.2939881201398 29.312969041422726 25.440721391432312 4 12.63153319198597 16.088954918263422 37.79885439552656 33.48065749422405 5 13.553441678481704 38.005347525091025 33.55484667491294 14.886364121514337 6 31.52769492134601 36.058765468708216 17.026165468164823 15.387374141780949 7 28.90841459299366 32.890891941187824 20.29845669100541 17.90223677481311 8 26.776011230807693 32.47585578226644 19.396747848800285 21.35138513812558 9 26.238128927093417 17.73926002318883 21.118841583643256 34.90376946607449 10-11 25.726438550609398 25.76093472399955 26.563994769471606 21.948631955919442 12-13 27.592338119582475 23.526277788776188 26.732191310221147 22.149192781420194 14-15 23.48809196155484 25.102065850864484 26.570234154609047 24.839608032971626 16-17 22.244343097361657 28.471585136273088 26.76423577315629 22.51983599320896 18-19 21.999994564926226 27.666156028545007 28.59299139472534 21.740858011803425 20-21 23.45553050207435 25.631794031924432 28.280268249046266 22.632407216954952 22-23 22.615862184316953 25.729335195567582 28.701918207349873 22.95288441276559 24-25 23.476065543231055 26.038725151555138 28.487786324719984 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22.05282200651142 26.958989230217938 28.32628650596421 22.661902257306433 52-53 23.11647579615499 27.461626228788134 26.750481678933614 22.67141629612327 54-55 22.31619652783544 26.519780131942568 27.435625423427606 23.728397916794393 56-57 23.22886816357177 26.55181512517001 26.963829428096364 23.255487283161855 58-59 24.270536263598956 26.11269763878247 26.74713624695222 22.869629850666346 60-61 23.030450838175582 26.707845446091476 28.29391916472113 21.967784551011803 62-63 22.418992031036158 26.917069926425707 27.99332821910324 22.67060982343489 64-65 22.125352493866828 27.815251016375502 27.23616676803745 22.823229721720217 66-67 22.74259515101684 26.949034787836858 26.491092296830704 23.8172777643156 68-69 22.39618327349488 26.754143016653554 27.328436960083664 23.52123674976791 70-71 23.462992381412104 26.685177007804846 26.901736746241344 22.95009386454171 72-73 23.13685773588948 26.093442180123482 28.071989002510296 22.697711081476744 74-75 22.150964118903794 28.25606734181833 26.854354689419242 22.738613849858638 76-77 22.692292540322306 26.834198959540778 26.798432809998683 23.675075690138225 78-79 23.020661192307127 26.967753854083142 27.080401518579784 22.93118343502995 80-81 23.50419166091835 27.053962358972267 26.718590678320464 22.723255301788914 82-83 23.149828031079547 26.78512703116278 27.457461774821702 22.607583162935967 84-85 22.70654372426237 27.339583929532857 27.028069477656462 22.925802868548313 86-87 22.772078220530087 27.49089643421928 26.74520822327149 22.991817121979146 88-89 22.454231400667947 27.51346546125195 26.061279343957217 23.971023794122885 90-91 22.90691948512763 26.608219471507283 26.681307568760438 23.80355347460465 92-93 23.56605379642391 27.024079341275247 26.946098605757225 22.46376825654362 94-95 22.90129703057709 26.65045176324947 27.229783679476572 23.218467526696866 96-97 22.023520207785314 26.76359356363804 28.062465207582548 23.150421020994102 98-99 22.479802558756546 27.374868235370826 26.468176222286736 23.67715298358589 100-101 22.19121776557953 26.557768579501495 27.00064287891774 24.250370776001233 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 335.0 1 235.5 2 246.5 3 352.0 4 301.0 5 297.5 6 474.0 7 896.5 8 1461.0 9 1876.0 10 1831.0 11 1648.5 12 1777.5 13 1978.0 14 2205.5 15 2389.5 16 2515.5 17 2771.0 18 3037.0 19 3265.0 20 3670.5 21 4244.5 22 4982.0 23 5972.5 24 7330.5 25 9258.0 26 11979.0 27 15213.0 28 17737.0 29 20938.0 30 25577.5 31 31090.5 32 38245.0 33 43573.0 34 48408.0 35 53280.0 36 59406.5 37 65930.0 38 67391.5 39 67096.0 40 67825.5 41 70745.5 42 73929.5 43 77087.5 44 80305.0 45 84244.5 46 88068.5 47 105907.0 48 144871.0 49 144242.0 50 108069.0 51 89781.5 52 83526.5 53 82421.0 54 81584.0 55 76852.0 56 69905.5 57 63436.5 58 58604.0 59 53174.5 60 46354.5 61 39765.5 62 34395.5 63 27643.0 64 20842.0 65 16755.5 66 12758.0 67 9924.0 68 7522.0 69 5132.0 70 3960.5 71 3380.5 72 2973.0 73 2633.0 74 2105.5 75 1719.5 76 1311.0 77 800.5 78 491.0 79 332.5 80 243.0 81 173.5 82 103.0 83 63.5 84 49.5 85 38.0 86 26.5 87 15.0 88 9.5 89 7.5 90 6.5 91 4.5 92 4.5 93 4.0 94 4.0 95 3.0 96 1.0 97 1.0 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07403417988908842 2 0.03003273335123398 3 0.015132772618838827 4 0.02161270345818776 5 0.02595852533846968 6 0.030149139294455816 7 0.07787557601540904 8 0.054943605200707124 9 0.07023158574384174 10-11 0.05975505085387639 12-13 0.04105249597623456 14-15 0.0448162881404073 16-17 0.055913654727555766 18-19 0.05145142690405201 20-21 0.01854734695334605 22-23 0.013774703281250727 24-25 0.022039525250001163 26-27 0.025221287698064716 28-29 0.006499331829885906 30-31 0.001067054479533507 32-33 0.009758698240097346 34-35 0.010883955691241771 36-37 0.0042488169275970555 38-39 0.009700495268486427 40-41 0.004054807022227326 42-43 0.0019206980631603127 44-45 3.8801981073945715E-4 46-47 4.2682179181340285E-4 48-49 7.760396214789143E-4 50-51 9.118465552377243E-4 52-53 1.746089148327557E-4 54-55 2.3281188644367425E-4 56-57 0.0036085842398769514 58-59 0.004132410984375218 60-61 0.005141262492297806 62-63 0.0025221287698064714 64-65 6.014307066461585E-4 66-67 3.2981683912853855E-4 68-69 0.004287618908671001 70-71 0.0027161386751761996 72-73 0.02328118864436743 74-75 0.01874135685871578 76-77 0.028306045193443395 78-79 0.022117129212149055 80-81 0.013406084461048243 82-83 0.004792044662632296 84-85 0.003899599097931544 86-87 0.0014938762713469099 88-89 0.0015132772618838828 90-91 0.00624711895290526 92-93 0.010457133899428369 94-95 0.013212074555678514 96-97 0.01154358936949885 98-99 0.024212436190142125 100-101 0.01930398558428799 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2577188.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.67026555937925 #Duplication Level Percentage of deduplicated Percentage of total 1 75.67587090321439 36.07488862393531 2 13.568723327298521 12.936492886281293 3 4.472770709558431 6.396545025325909 4 1.9958790506593975 3.80576337477341 5 1.0874435716695678 2.591936192116408 6 0.6856752653236801 1.9611793191286597 7 0.44542422252030217 1.4863443681885988 8 0.302930870297717 1.1552636026580834 9 0.22718078714846496 0.9746791608020524 >10 1.2992067910325944 11.740665151732227 >50 0.1390748986666972 4.642103886033085 >100 0.09151524368887858 7.935271326724537 >500 0.005015503903036639 1.649762975653816 >1k 0.0023844198883286325 2.3858478556227016 >5k 6.577710036768642E-4 2.2211498436083335 >10k+ 2.466641263788241E-4 2.0421064074156554 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 26519 1.0289897360999662 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 15200 0.5897901123239748 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10382 0.4028421675097044 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 9469 0.3674159587891919 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8043 0.31208433377774536 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 8004 0.3105710565158615 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6654 0.2581883820660347 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6573 0.25504542159904514 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6443 0.25000116405943223 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6034 0.23413115380018842 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5449 0.21143199487193018 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4984 0.19338907367254543 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4840 0.18780158839789726 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4168 0.16172665711620574 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3884 0.15070689449120514 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2940 0.1140778243574004 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2884 0.11190491341725943 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2766 0.10732627965053385 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2711 0.10519217069146683 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 1.9400990536972856E-5 0.0 3.880198107394571E-5 0.0 12-13 0.0 3.880198107394571E-5 0.0 3.880198107394571E-5 0.0 14-15 0.0 3.880198107394571E-5 0.0 3.880198107394571E-5 0.0 16-17 0.0 3.880198107394571E-5 0.0 7.760396214789142E-5 0.0 18-19 0.0 3.880198107394571E-5 0.0 7.760396214789142E-5 0.0 20-21 0.0 3.880198107394571E-5 0.0 7.760396214789142E-5 0.0 22-23 0.0 3.880198107394571E-5 0.0 1.3580693375881E-4 1.9400990536972856E-5 24-25 0.0 3.880198107394571E-5 0.0 2.3281188644367427E-4 3.880198107394571E-5 26-27 0.0 3.880198107394571E-5 0.0 2.7161386751762E-4 3.880198107394571E-5 28-29 0.0 3.880198107394571E-5 0.0 5.238267444982671E-4 3.880198107394571E-5 30-31 0.0 3.880198107394571E-5 0.0 9.700495268486428E-4 3.880198107394571E-5 32-33 0.0 3.880198107394571E-5 0.0 0.0016878861767166383 3.880198107394571E-5 34-35 0.0 3.880198107394571E-5 0.0 0.0023475198549737157 3.880198107394571E-5 36-37 0.0 3.880198107394571E-5 0.0 0.003375772353433277 3.880198107394571E-5 38-39 0.0 3.880198107394571E-5 0.0 0.004384623861355866 3.880198107394571E-5 40-41 0.0 3.880198107394571E-5 0.0 0.0057620941894809385 3.880198107394571E-5 42-43 0.0 3.880198107394571E-5 0.0 0.008109614044454653 3.880198107394571E-5 44-45 0.0 3.880198107394571E-5 0.0 0.01406571813930532 3.880198107394571E-5 46-47 0.0 3.880198107394571E-5 0.0 0.02621073821545033 3.880198107394571E-5 48-49 0.0 3.880198107394571E-5 0.0 0.03579482754071492 3.880198107394571E-5 50-51 0.0 3.880198107394571E-5 0.0 0.04875468921941278 3.880198107394571E-5 52-53 0.0 3.880198107394571E-5 0.0 0.0640426697625474 5.820297161091857E-5 54-55 0.0 3.880198107394571E-5 0.0 0.08404509100616642 7.760396214789142E-5 56-57 0.0 3.880198107394571E-5 0.0 0.10810231927201275 9.700495268486427E-5 58-59 0.0 3.880198107394571E-5 0.0 0.14271368638997234 1.1640594322183714E-4 60-61 0.0 3.880198107394571E-5 0.0 0.1854734695334605 1.1640594322183714E-4 62-63 0.0 3.880198107394571E-5 0.0 0.2347907874784455 1.1640594322183714E-4 64-65 0.0 3.880198107394571E-5 0.0 0.27400018935366766 1.1640594322183714E-4 66-67 0.0 3.880198107394571E-5 0.0 0.33740262642849495 1.1640594322183714E-4 68-69 0.0 3.880198107394571E-5 0.0 0.4185763708351894 1.1640594322183714E-4 70-71 0.0 3.880198107394571E-5 0.0 0.4742378126857645 1.5520792429578285E-4 72-73 0.0 3.880198107394571E-5 0.0 0.5661403048594049 1.5520792429578285E-4 74-75 0.0 3.880198107394571E-5 0.0 0.6381761827231851 1.5520792429578285E-4 76-77 0.0 3.880198107394571E-5 0.0 0.704702179274465 1.5520792429578285E-4 78-79 0.0 3.880198107394571E-5 0.0 0.7971867011642146 1.5520792429578285E-4 80-81 0.0 3.880198107394571E-5 0.0 0.8911068963537002 1.746089148327557E-4 82-83 0.0 3.880198107394571E-5 0.0 1.012770508011057 1.9400990536972855E-4 84-85 0.0 5.820297161091857E-5 0.0 1.1775431206415674 1.9400990536972855E-4 86-87 0.0 7.760396214789142E-5 0.0 1.4046511158673716 1.9400990536972855E-4 88-89 0.0 7.760396214789142E-5 0.0 1.6820658795555465 1.9400990536972855E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 13570 0.0 27.039236 1 TACATGG 13835 0.0 25.86809 2 ACATGGG 13690 0.0 24.790993 3 GTATAGG 890 0.0 24.565453 1 GAGTACT 8910 0.0 22.220474 12-13 CATTCCG 450 0.0 22.186575 9 GTAAGCG 225 0.0 22.169132 94-95 CATGGGA 6715 0.0 21.37807 4 GTACTTT 9675 0.0 20.41021 14-15 AGTACTT 9335 0.0 20.215858 12-13 CATGGGG 6160 0.0 19.986036 4 ATGGGAG 2910 0.0 19.276596 5 AGAGTAC 17175 0.0 18.849697 10-11 CGTACAC 290 5.178481E-8 18.02992 3 ACTTTTT 11295 0.0 17.83773 16-17 GTACTAA 455 0.0 17.758017 1 ATGGGTA 1085 0.0 17.525547 5 CATGGGT 2225 0.0 17.518278 4 TACTTTT 11105 0.0 17.033031 14-15 GTACCTG 2105 0.0 16.708466 1 >>END_MODULE