FastQCFastQC Report
Thu 26 May 2016
SRR936744_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936744_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2577188
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT278241.0796263214014656No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT153450.5954163995796969No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT110050.42701580171877257No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC100550.39015391969852414No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86000.33369703723593314No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT77850.3020734226606674No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76130.29539948191594867No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC71540.2775893726030076No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT69230.26862611497492617No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC67490.2618745702680596No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG57170.2218309257997476No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA52780.20479685610828546No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC52060.2020031134709614No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA45190.17534615247316065No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44630.17317324153301972No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC33160.12866736924120398No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT32480.12602883452817568No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA30620.11881166604842176No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC28890.11209892332262915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG131250.025.8451
TACATGG134650.024.9768162
ACATGGG133800.024.5991383
CATGGGG60050.020.9576194
GAGTACT103400.020.82847212-13
AGTACTT105600.020.43951612-13
GTAAGGT8600.020.432074
GTGTACG2108.600218E-720.3610821
TAAGGTG10700.019.9727485
CATGGGA66300.019.6266464
GTACTTT109400.019.51250514-15
AGAGTAC185650.018.58409110-11
TACATCG2302.0637144E-618.583345
GTATAGA5700.018.3368821
GGTAAGG8100.018.1768863
TCGCGCT6200.018.0069960-61
CATGGGT21100.017.78094
GTAAGCG2950.017.72062194-95
CATACGT1350.00941402117.5891694
TACTTTT120800.017.49418414-15
Produced by FastQC (version 0.11.5)