##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936744_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2577188 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.79535291953866 31.0 31.0 34.0 30.0 34.0 2 31.950128589765278 31.0 31.0 34.0 30.0 34.0 3 32.036141329231704 33.0 31.0 34.0 30.0 34.0 4 35.7359777400795 37.0 35.0 37.0 35.0 37.0 5 35.55391224854376 37.0 35.0 37.0 33.0 37.0 6 35.651115479351915 37.0 35.0 37.0 33.0 37.0 7 35.59187765890575 37.0 35.0 37.0 33.0 37.0 8 35.56342533024366 37.0 35.0 37.0 33.0 37.0 9 37.16448353787151 39.0 37.0 39.0 33.0 39.0 10-11 37.047223175026424 39.0 37.0 39.0 33.0 39.0 12-13 36.94549524520524 39.0 37.0 39.0 33.0 39.0 14-15 38.02137368325477 40.0 37.0 41.0 33.0 41.0 16-17 37.93393070276596 40.0 37.0 41.0 33.0 41.0 18-19 38.09289388279008 40.0 37.0 41.0 33.0 41.0 20-21 38.11294480651004 40.0 37.0 41.0 33.0 41.0 22-23 38.02470289323092 40.0 37.0 41.0 33.0 41.0 24-25 37.871081193921434 40.0 37.0 41.0 32.5 41.0 26-27 37.65568829282148 39.5 37.0 41.0 32.0 41.0 28-29 37.59047457927012 39.0 36.5 41.0 32.0 41.0 30-31 37.58961880157753 39.0 37.0 41.0 32.0 41.0 32-33 37.43244051268282 39.0 36.0 41.0 32.0 41.0 34-35 37.237808611556474 39.0 36.0 41.0 31.0 41.0 36-37 37.01660802393927 39.0 35.5 41.0 31.0 41.0 38-39 36.85283010009359 39.0 35.0 41.0 31.0 41.0 40-41 36.6863707653458 39.0 35.0 41.0 30.0 41.0 42-43 36.394713928514335 39.0 35.0 40.0 30.0 41.0 44-45 36.1176982432015 39.0 35.0 40.0 30.0 41.0 46-47 35.753189328834374 38.0 35.0 40.0 28.5 41.0 48-49 35.358197578135545 38.0 34.5 40.0 27.0 41.0 50-51 35.06556991573762 38.0 34.0 40.0 26.5 41.0 52-53 34.725892911188474 38.0 34.0 40.0 26.0 41.0 54-55 34.535563373723605 38.0 33.5 40.0 26.0 41.0 56-57 34.198726480179175 37.5 33.0 40.0 24.5 41.0 58-59 33.81520284899666 37.0 33.0 40.0 23.5 41.0 60-61 33.34448786817259 36.5 32.0 39.0 22.0 41.0 62-63 33.04545846092718 36.0 32.0 39.0 22.0 40.0 64-65 32.65092243949607 35.5 31.0 39.0 21.5 40.0 66-67 32.188337249746624 35.0 31.0 38.0 20.5 40.0 68-69 31.80467567752139 35.0 31.0 38.0 20.0 40.0 70-71 31.40927747607082 35.0 30.5 37.0 18.5 39.0 72-73 30.93988389671223 34.0 30.0 37.0 17.5 39.0 74-75 30.45128876899939 34.0 29.5 36.0 13.5 39.0 76-77 28.95414226668757 32.5 28.0 34.5 11.0 36.5 78-79 29.74651597011937 34.0 29.0 35.0 9.5 37.0 80-81 29.498774633437684 34.0 29.0 35.0 7.0 37.0 82-83 29.19768542302696 34.0 29.0 35.0 6.0 36.0 84-85 28.806898837027024 33.5 29.0 35.0 2.0 36.0 86-87 28.470098999374514 33.0 28.5 35.0 2.0 35.5 88-89 27.998238778079052 33.0 27.0 35.0 2.0 35.0 90-91 27.507702193243176 33.0 26.0 35.0 2.0 35.0 92-93 26.901301340841258 32.0 25.0 34.0 2.0 35.0 94-95 26.326574351580092 32.0 25.0 34.0 2.0 35.0 96-97 25.572470848071617 32.0 23.5 34.0 2.0 35.0 98-99 24.69793705387422 32.0 18.0 34.0 2.0 35.0 100-101 21.503804728254206 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.33189606115874426 1101 2 0.3222275506846195 1101 3 0.2953487551444738 1101 4 0.2074064158539528 1101 5 0.21605816576064996 1101 6 0.23747897862176615 1101 7 0.2515702409695564 1101 8 0.2660422372642657 1101 9 0.2607298685116035 1101 10-11 0.2948558735650977 1101 12-13 0.3880699560552969 1101 14-15 0.49073501909742134 1101 16-17 0.43463013745398626 1101 18-19 0.33603114459544514 1101 20-21 0.43715332841608046 1101 22-23 0.36251671673240793 1101 24-25 0.37485021002520114 1101 26-27 0.3775787010978391 1101 28-29 0.21969020593255806 1101 30-31 0.29027482670117877 1101 32-33 0.34173947391038695 1101 34-35 0.3761896252327688 1101 36-37 0.29954425889776104 1101 38-39 0.3879228759115563 1101 40-41 0.3726355937439294 1101 42-43 0.42111028446988996 1101 44-45 0.5415021296268208 1101 46-47 0.37634340942346256 1101 48-49 0.2053967977381177 1101 50-51 0.26599920431211643 1101 52-53 0.34604094386866535 1101 54-55 0.1235780935231503 1101 56-57 0.1301821614024945 1101 58-59 0.074637714694191 1101 60-61 0.25422301303279227 1101 62-63 0.16913463314752164 1101 64-65 0.23729419981517452 1101 66-67 0.17922832243237252 1101 68-69 0.08521687523547783 1101 70-71 0.048745580381723386 1101 72-73 0.1463488759059004 1101 74-75 0.0192107887259354 1101 76-77 -0.09135541425223437 1101 78-79 -0.036401903288936666 1101 80-81 0.01320571873811005 1101 82-83 0.06975669487944813 1101 84-85 0.1479526371230655 1101 86-87 0.13658340744629172 1101 88-89 -0.08808949196126292 1101 90-91 -0.048762699786824015 1101 92-93 -0.07997712997435613 1101 94-95 0.0062150321674501185 1101 96-97 0.1564238906726878 1101 98-99 0.07854470087373144 1101 100-101 0.754446046754218 1102 1 0.29517757395777977 1102 2 0.2880424873984886 1102 3 0.2835199541841291 1102 4 0.25979869092271457 1102 5 0.22035133323309708 1102 6 0.22862353746566555 1102 7 0.28473593256592267 1102 8 0.24221380566539352 1102 9 0.30873069957490173 1102 10-11 0.32371718493021717 1102 12-13 0.40681752387600056 1102 14-15 0.4832828217264833 1102 16-17 0.3871506996749119 1102 18-19 0.33349838025464607 1102 20-21 0.30067558574230446 1102 22-23 0.26141968019860684 1102 24-25 0.36890711807398446 1102 26-27 0.4356584831745778 1102 28-29 0.38072075740242184 1102 30-31 0.36424902416445804 1102 32-33 0.34419539787469233 1102 34-35 0.30584423928301874 1102 36-37 0.4081968810343781 1102 38-39 0.3725111038339648 1102 40-41 0.4042588714311961 1102 42-43 0.5115932905445604 1102 44-45 0.3071663536709295 1102 46-47 0.26584760761226534 1102 48-49 0.4513808162589541 1102 50-51 0.3490512842788718 1102 52-53 0.32212231551886106 1102 54-55 0.3393387802716603 1102 56-57 0.18063842174956335 1102 58-59 0.34135544338500523 1102 60-61 0.4296189223545568 1102 62-63 0.46764069793088225 1102 64-65 0.23957137921271965 1102 66-67 0.12650362204387733 1102 68-69 0.2897417180919639 1102 70-71 0.23325927643681155 1102 72-73 0.21574645394999692 1102 74-75 0.2210777130661974 1102 76-77 0.21712011225274708 1102 78-79 0.34483156613650934 1102 80-81 0.421684213326305 1102 82-83 0.39540043914939815 1102 84-85 0.40192195373839823 1102 86-87 0.4767999456059471 1102 88-89 0.30726150036789335 1102 90-91 0.2819149136104464 1102 92-93 0.33583681737844273 1102 94-95 0.2869663529644839 1102 96-97 0.4548539395471245 1102 98-99 0.5161127194473387 1102 100-101 1.186741595288975 1103 1 0.18015622566649014 1103 2 0.2247199146856893 1103 3 0.16402455244696057 1103 4 0.15471346441825773 1103 5 0.2834776865650852 1103 6 0.19077206421658843 1103 7 0.10105509596698425 1103 8 0.09055037473178373 1103 9 0.20621078991128883 1103 10-11 0.20769477189723062 1103 12-13 0.24164357099255085 1103 14-15 0.3376907140221661 1103 16-17 0.2835724946168483 1103 18-19 0.26730389791848097 1103 20-21 0.17052899351322282 1103 22-23 0.18779480385769176 1103 24-25 0.21040824036079186 1103 26-27 0.1337392752468105 1103 28-29 0.2107059403235425 1103 30-31 0.1454975894674675 1103 32-33 0.1998095362357688 1103 34-35 0.2336369221868324 1103 36-37 0.041382043578870764 1103 38-39 0.1769877672491731 1103 40-41 0.18104203077705705 1103 42-43 0.18255744465979262 1103 44-45 0.08704264112477489 1103 46-47 0.20415375009647363 1103 48-49 0.11676466727813306 1103 50-51 0.10335128736052468 1103 52-53 0.03769556226409776 1103 54-55 0.013008340545283659 1103 56-57 -0.2807698799654048 1103 58-59 0.03317293797795884 1103 60-61 -0.009411722458814609 1103 62-63 0.06676796192204648 1103 64-65 0.23687902290097185 1103 66-67 0.08529739484573184 1103 68-69 0.17358361809931822 1103 70-71 0.14580596501464171 1103 72-73 0.21363505165435726 1103 74-75 0.10390329730626036 1103 76-77 0.059384414727439605 1103 78-79 -0.12840445621992203 1103 80-81 0.10198812695958992 1103 82-83 0.03521869723700277 1103 84-85 0.32672716512389854 1103 86-87 0.39055445251985077 1103 88-89 0.194789588983582 1103 90-91 0.2055898196043806 1103 92-93 0.32991580322611824 1103 94-95 0.13704232508372272 1103 96-97 0.21495813706960476 1103 98-99 0.21736195139310865 1103 100-101 0.6935037742548857 1104 1 0.15675591643658748 1104 2 0.1642440043647504 1104 3 0.16676738341922004 1104 4 0.14416634512319604 1104 5 0.1415613699643643 1104 6 0.08559533322866741 1104 7 0.05162165871996649 1104 8 0.08504109604102439 1104 9 0.18136657075266527 1104 10-11 0.18482972575787926 1104 12-13 0.18752818853878495 1104 14-15 0.2190370419469687 1104 16-17 0.2728962305828233 1104 18-19 0.11351299162136286 1104 20-21 0.2105465237157489 1104 22-23 0.204316212587635 1104 24-25 0.19001248946186422 1104 26-27 0.14136524218815083 1104 28-29 0.11348715598209935 1104 30-31 0.17980943335538768 1104 32-33 0.17125733844341795 1104 34-35 0.15612541574932948 1104 36-37 0.16207482737741685 1104 38-39 0.2183242478876366 1104 40-41 0.2032658374139089 1104 42-43 0.15339091251889414 1104 44-45 0.13186976654225901 1104 46-47 0.24882049090178526 1104 48-49 0.3646460358775414 1104 50-51 0.30174645471656447 1104 52-53 0.3159960473939165 1104 54-55 0.372468222569168 1104 56-57 0.22693186982622393 1104 58-59 0.30627938313748615 1104 60-61 0.4147909283565383 1104 62-63 0.34579473673547767 1104 64-65 0.3958176325143583 1104 66-67 0.42033681388573285 1104 68-69 0.3624228221372441 1104 70-71 0.44111035796860065 1104 72-73 0.5728258821387371 1104 74-75 0.2956636536979076 1104 76-77 0.25766075098685803 1104 78-79 0.20941695573864294 1104 80-81 0.4579545242159817 1104 82-83 0.39397351475428977 1104 84-85 0.7430428690045794 1104 86-87 0.7353493814364356 1104 88-89 0.6930972548000511 1104 90-91 0.8614186418402348 1104 92-93 0.8299261168684673 1104 94-95 0.7948507687580779 1104 96-97 0.9066841146534266 1104 98-99 1.0571451974939556 1104 100-101 1.7574229463892799 1105 1 0.05897186898998186 1105 2 0.09430314313521748 1105 3 0.08626707665238342 1105 4 0.08692156326662115 1105 5 0.11744090946384489 1105 6 0.08719498386474811 1105 7 -0.006598223612641618 1105 8 0.17229196803846492 1105 9 0.004756440328741007 1105 10-11 0.1279604931721252 1105 12-13 0.1515812450776579 1105 14-15 0.11039680995666856 1105 16-17 0.021217769682316145 1105 18-19 0.11467523116615297 1105 20-21 0.17022871779351334 1105 22-23 0.2716022413073418 1105 24-25 0.2482045480989612 1105 26-27 -0.02416526670582897 1105 28-29 0.09316992630695609 1105 30-31 0.10786865573818716 1105 32-33 0.1322254957069049 1105 34-35 0.24463343857775044 1105 36-37 0.20599460135297676 1105 38-39 0.12175625129539469 1105 40-41 0.24795789548605285 1105 42-43 0.21171968860396362 1105 44-45 0.19725248500487425 1105 46-47 0.16288058104490943 1105 48-49 0.21911115993449926 1105 50-51 0.22342783639891195 1105 52-53 0.35028317324289304 1105 54-55 0.13651862385182767 1105 56-57 0.07144063047691418 1105 58-59 0.1757735398789535 1105 60-61 0.26942765540862723 1105 62-63 0.2757812796709729 1105 64-65 0.4187646957444784 1105 66-67 0.20833240051092616 1105 68-69 0.3222055769825154 1105 70-71 0.2901960459115891 1105 72-73 0.3429503232637927 1105 74-75 0.170887373158223 1105 76-77 0.2004831979580004 1105 78-79 0.1936873873703142 1105 80-81 0.2244350498889176 1105 82-83 0.3506612012577932 1105 84-85 0.4529273145136372 1105 86-87 0.5654262595782527 1105 88-89 0.4913681180520939 1105 90-91 0.4223379875851023 1105 92-93 0.40495730200536073 1105 94-95 0.5986136340394204 1105 96-97 0.7516475173134296 1105 98-99 0.673092186150928 1105 100-101 1.1867326814625017 1106 1 0.12900977172201777 1106 2 0.1706788435941924 1106 3 0.05558968213508564 1106 4 0.12606663046684474 1106 5 0.22626191643762184 1106 6 0.01979957613539085 1106 7 0.05417916144895685 1106 8 0.1585555702508259 1106 9 0.13783186012169324 1106 10-11 0.12419441056241709 1106 12-13 0.19307445393028644 1106 14-15 0.1411584924972331 1106 16-17 0.11326614921578226 1106 18-19 0.1573331723109206 1106 20-21 0.023881690424651936 1106 22-23 0.06758883572108942 1106 24-25 0.12109173061292466 1106 26-27 0.08583046582532461 1106 28-29 -0.11238981646501855 1106 30-31 -0.023023890333526253 1106 32-33 0.06761372923669029 1106 34-35 0.09694448798638433 1106 36-37 -0.19645569214578273 1106 38-39 0.047388990390132335 1106 40-41 0.19267928738662476 1106 42-43 0.16752361164286356 1106 44-45 0.13690618872950466 1106 46-47 0.29284678588947344 1106 48-49 0.11152545838109518 1106 50-51 0.2793388458896118 1106 52-53 0.12035509442814174 1106 54-55 0.2477037617023683 1106 56-57 -0.04574280975909062 1106 58-59 -0.035520062804806685 1106 60-61 -0.11587529766327265 1106 62-63 -0.10919341186794895 1106 64-65 -0.16961126219614187 1106 66-67 -0.08691124632239422 1106 68-69 -0.04999131212090546 1106 70-71 0.08658884932711075 1106 72-73 0.11944584641881306 1106 74-75 0.08674332182509659 1106 76-77 -0.07423579831568361 1106 78-79 -0.16115857437188197 1106 80-81 0.17452124007854053 1106 82-83 0.12156035804674659 1106 84-85 0.4247994431728621 1106 86-87 0.5591044510756795 1106 88-89 0.3992069180001572 1106 90-91 0.515479423577343 1106 92-93 0.6100423302194464 1106 94-95 0.735753831693799 1106 96-97 0.9310799715242872 1106 98-99 0.9890828853845157 1106 100-101 1.2156101855335528 1107 1 -0.040830619558299475 1107 2 0.05955675573328634 1107 3 0.003838181841764765 1107 4 0.00644084716892479 1107 5 -0.06672558474256363 1107 6 -0.10405882715151904 1107 7 -0.004976208153088635 1107 8 -0.0022976476489446895 1107 9 0.033368931714612415 1107 10-11 0.02556670024819141 1107 12-13 -0.04543989499492085 1107 14-15 0.04986085679190211 1107 16-17 0.045000084646680705 1107 18-19 0.022918414007456533 1107 20-21 -0.011636898679036278 1107 22-23 0.08908643185709053 1107 24-25 0.008611369418034087 1107 26-27 0.0546536969537712 1107 28-29 0.007496867231672866 1107 30-31 0.023166619071353978 1107 32-33 -0.001077665969795305 1107 34-35 0.12040390925560018 1107 36-37 0.1849121340546347 1107 38-39 0.019122703044573086 1107 40-41 0.011888240029655606 1107 42-43 0.050996144897560214 1107 44-45 0.05781822756899402 1107 46-47 0.009170080668340574 1107 48-49 0.10084631110570541 1107 50-51 0.002908674107757747 1107 52-53 0.026732826224396433 1107 54-55 0.016353200677009738 1107 56-57 -0.17564203523962618 1107 58-59 -0.0462566943611975 1107 60-61 0.15372690846010784 1107 62-63 0.16151279744219948 1107 64-65 0.08566853972376975 1107 66-67 0.13918179042829593 1107 68-69 0.13515441815621898 1107 70-71 0.008212711973758502 1107 72-73 -0.018825530307839955 1107 74-75 -0.039008681306505366 1107 76-77 -0.056052068210416195 1107 78-79 0.18043457164535326 1107 80-81 0.31757919431176873 1107 82-83 0.32266583314935815 1107 84-85 0.40400949378554785 1107 86-87 0.3299087881088738 1107 88-89 0.4161578854334209 1107 90-91 0.548088989810374 1107 92-93 0.46879622482921235 1107 94-95 0.5887655306400674 1107 96-97 0.9390421743166293 1107 98-99 0.7660609811550536 1107 100-101 1.1171840348666535 1108 1 0.11174366933821389 1108 2 0.16712162232185435 1108 3 0.11870945838160907 1108 4 0.13593099676158715 1108 5 0.0470328136814544 1108 6 0.14620673871757361 1108 7 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-1.143678612541585 2316 48-49 -1.3816837976732046 2316 50-51 -1.2694599838991962 2316 52-53 -1.0734512523865334 2316 54-55 -0.9398600190628059 2316 56-57 -0.8650200564477473 2316 58-59 -1.5251144047865282 2316 60-61 -1.0976755365059532 2316 62-63 -0.8333178453019059 2316 64-65 -0.5951725448830061 2316 66-67 -0.8331808236205767 2316 68-69 -1.3834974369072022 2316 70-71 -1.2361829816444363 2316 72-73 -0.808021986081485 2316 74-75 -0.9425828807595877 2316 76-77 -0.8400732306979108 2316 78-79 -1.191021001562401 2316 80-81 -0.9616276934568617 2316 82-83 -1.2372021927089172 2316 84-85 -1.0463598968971333 2316 86-87 -1.0314657097183897 2316 88-89 -1.247227184386631 2316 90-91 -1.3548963862780035 2316 92-93 -1.326002147495874 2316 94-95 -1.4219456890430067 2316 96-97 -1.3674049250152045 2316 98-99 -2.2548799459387787 2316 100-101 -3.252550552115153 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 21.0 4 56.0 5 101.0 6 217.0 7 543.0 8 1121.0 9 2086.0 10 3484.0 11 5340.0 12 7476.0 13 10279.0 14 13149.0 15 17325.0 16 21655.0 17 26863.0 18 23682.0 19 17374.0 20 16617.0 21 17533.0 22 18789.0 23 21437.0 24 25226.0 25 30386.0 26 37276.0 27 45708.0 28 56787.0 29 69776.0 30 85178.0 31 103970.0 32 128689.0 33 160504.0 34 207562.0 35 274994.0 36 379519.0 37 466717.0 38 265208.0 39 14538.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.63779467662901 19.342414417547207 14.882765836722117 22.137025069101675 2 15.780811477038473 21.3499366664183 38.05832122544272 24.810930631100515 3 20.91703965050373 24.269121129265965 29.6826190178683 25.131220202362 4 12.981668392061424 16.75589828914305 37.279701752452674 32.98273156634285 5 13.475214457253482 38.27104013174565 33.557384752629616 14.696360658371255 6 31.715575270411005 36.3100014434337 16.64213088063424 15.332292405521056 7 28.788403790644608 33.045487192043105 20.124189668813322 18.041919348498965 8 26.801265565414706 32.768117809022854 19.2024019978364 21.228214627726032 9 25.946845942166423 17.56790734707751 21.006655315793804 35.47859139496226 10-11 25.812964714441616 25.848759548926882 26.406790348005337 21.931485388626168 12-13 27.521584283812693 23.57022611478623 26.80965844438723 22.09853115701384 14-15 23.34500499188455 25.21862048601128 26.632936039078324 24.803438483025847 16-17 22.129884249765343 28.60473454196378 26.905769740418528 22.359611467852353 18-19 21.926853448861245 27.741365963630436 28.80346738942749 21.528313198080827 20-21 23.24667428220215 25.612237058375253 28.56136222890996 22.579726430512636 22-23 22.435900545032343 25.68287161212431 28.975764850545353 22.905462992297995 24-25 23.376447507903965 25.98718060149279 28.768390198929993 21.86798169167325 26-27 22.72297886284607 25.5857985456384 29.844756473686285 21.846466117829248 28-29 21.63757496528572 27.38475024053111 29.10355574724318 21.87411904693999 30-31 22.126093357246017 25.84971639257879 28.98455039939406 23.039639850781132 32-33 22.19061293389472 26.158190884572846 29.15547449504259 22.49572168648984 34-35 23.53987365839652 25.783504194572277 28.617731283515834 22.05889086351537 36-37 23.07410094833072 25.287157180772635 29.4938614599016 22.14488041099505 38-39 22.381636137770098 26.356164574916814 29.201285839756615 22.060913447556477 40-41 22.48102336563282 26.463938816905667 28.836432809861158 22.218605007600353 42-43 22.195343610327907 26.625890855244315 27.848059268503363 23.330706265924412 44-45 22.725590094407345 25.825976958865095 28.04447195135674 23.40396099537082 46-47 23.65719403161132 26.77857330770323 27.58389214869908 21.980340511986366 48-49 23.40400392989514 26.620829530709678 27.487381497830178 22.487785041565004 50-51 22.088868280701618 26.905092183913844 28.42399750114951 22.582042034235027 52-53 23.18144982691376 27.47586658070719 26.768000803978452 22.574682788400594 54-55 22.371530628023333 26.533777830980565 27.51259486528 23.582096675716098 56-57 23.324329101972 26.503875131148657 27.07042180823413 23.101373958645215 58-59 24.300145297076924 26.163215950036587 26.81774172983671 22.71889702304978 60-61 23.14839970162021 26.68290782129193 28.314604386741816 21.854088090346046 62-63 22.506506166984764 26.965762775799 27.99696986866585 22.53076118855039 64-65 22.17000564490136 27.765831871978524 27.28984773514099 22.77431474797912 66-67 22.837650323774284 26.897110089464764 26.557484680478556 23.7077549062824 68-69 22.482455452797932 26.78772785793942 27.292160266712816 23.437656422549836 70-71 23.48337430180604 26.696996917179018 26.953381305571778 22.866247475443167 72-73 23.243841737583754 26.069013979577743 28.108193891947348 22.578950390891155 74-75 22.354075811858326 28.15451063445063 26.85339928750558 22.638014266185465 76-77 22.714000346122535 26.897446065805187 26.80202955079348 23.586524037278796 78-79 23.018638599688344 26.895783220312442 27.16403360881468 22.921544571184533 80-81 23.598237114058225 26.98093751503871 26.71536362239219 22.705461748510878 82-83 23.219326835530875 26.708389833994783 27.443778122581662 22.62850520789268 84-85 22.711835131416283 27.357972794898217 27.014173124433547 22.91601894925195 86-87 22.711897162635477 27.392658970046906 26.754650210887693 23.140793656429924 88-89 22.431840233627963 27.477507815710144 26.104287494689082 23.986364455972808 90-91 22.918786484976643 26.56201412405581 26.686553651962484 23.832645739005056 92-93 23.532222367991032 26.97979489034606 26.88392386520922 22.60405887645369 94-95 22.875506728170127 26.572448737196915 27.17555423757497 23.376490297057988 96-97 21.923650531164974 26.713656589475775 28.023773580290868 23.338919299068387 98-99 22.363187242488802 27.33378304272077 26.43961195579907 23.863417758991357 100-101 21.906129704769576 26.596848962625476 26.976823915689163 24.52019741691579 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 424.0 1 325.0 2 325.5 3 419.5 4 350.5 5 316.5 6 534.0 7 991.5 8 1482.0 9 1869.5 10 1827.0 11 1669.5 12 1789.5 13 1962.5 14 2215.0 15 2440.5 16 2667.0 17 2989.5 18 3252.5 19 3555.5 20 4039.0 21 4628.5 22 5497.5 23 6585.5 24 7744.5 25 9544.0 26 12396.5 27 15786.0 28 18409.5 29 21636.0 30 26235.0 31 31301.5 32 38036.0 33 43652.0 34 48662.5 35 53456.5 36 59762.5 37 65899.5 38 67200.0 39 67070.0 40 67455.5 41 70334.0 42 73233.0 43 76224.5 44 79797.5 45 83444.0 46 86062.5 47 105479.5 48 149364.0 49 146736.0 50 105992.5 51 87700.5 52 81706.0 53 81216.0 54 81010.0 55 76742.5 56 70115.0 57 63434.0 58 58802.0 59 53701.0 60 46436.0 61 39691.5 62 34079.5 63 27209.5 64 20672.5 65 16503.0 66 12480.5 67 9846.5 68 7503.0 69 5094.5 70 3931.5 71 3266.0 72 2863.0 73 2612.0 74 2171.0 75 1788.5 76 1303.0 77 784.5 78 504.0 79 362.0 80 257.0 81 186.5 82 134.0 83 95.0 84 63.0 85 32.5 86 12.0 87 7.0 88 5.5 89 2.5 90 1.5 91 1.0 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021341089590670142 2 0.013813505262324673 3 0.008614039798415948 4 0.0 5 0.003919000088468517 6 0.0 7 3.880198107394571E-5 8 0.0 9 0.0 10-11 1.9400990536972856E-5 12-13 9.118465552377243E-4 14-15 1.9400990536972857E-4 16-17 3.880198107394571E-5 18-19 0.0012804653754402084 20-21 0.0 22-23 5.820297161091856E-5 24-25 0.0 26-27 0.0016878861767166385 28-29 0.0030459555143047385 30-31 0.0013192673565141542 32-33 0.00923487149559908 34-35 0.001862495091549394 36-37 0.00108645547007048 38-39 0.0015326782524208557 40-41 2.1341089590670142E-4 42-43 0.006596336782570771 44-45 0.0022505149022888516 46-47 3.8801981073945715E-4 48-49 7.760396214789143E-4 50-51 1.1640594322183712E-4 52-53 1.746089148327557E-4 54-55 0.0018042921199384757 56-57 0.016937064738777305 58-59 0.02881047094740469 60-61 0.023514000530811104 62-63 0.015598396391726176 64-65 0.020429243035432417 66-67 0.0032593664102114396 68-69 0.0013192673565141542 70-71 1.1640594322183712E-4 72-73 0.0 74-75 0.001746089148327557 76-77 0.0025609307508804168 78-79 0.011815203237016469 80-81 0.019556198461268638 82-83 0.020254634120599663 84-85 0.03232205023459678 86-87 0.013289678517826406 88-89 0.04371043167979984 90-91 0.05040377341505548 92-93 0.03142960466989603 94-95 0.03321449579929753 96-97 0.05383774874009968 98-99 0.05628227354775825 100-101 0.06299501627355086 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2577188.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.88524791701952 #Duplication Level Percentage of deduplicated Percentage of total 1 73.40450907873918 32.94779588226319 2 14.517856011375303 13.032751325883455 3 5.095585640438733 6.861498742604917 4 2.277285757058109 4.0886614313380285 5 1.2207622750506775 2.739710868169722 6 0.706388477711472 1.9023853168683265 7 0.4770994576724595 1.4990309207092736 8 0.3555169382644803 1.2765972730160737 9 0.24536376302524313 0.9911891999916779 >10 1.4314426901928075 12.197886062786813 >50 0.15514355749044154 4.865212014732559 >100 0.10344532332335205 8.570466971912595 >500 0.005622224574351714 1.7565457046499964 >1k 0.002854360168516828 2.407427340633809 >5k 7.784618641409531E-4 2.369224684516412 >10k+ 3.459830507293125E-4 2.4936162599230585 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 27824 1.0796263214014656 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 15345 0.5954163995796969 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 11005 0.42701580171877257 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 10055 0.39015391969852414 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8600 0.33369703723593314 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 7785 0.3020734226606674 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7613 0.29539948191594867 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 7154 0.2775893726030076 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6923 0.26862611497492617 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6749 0.2618745702680596 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5717 0.2218309257997476 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5278 0.20479685610828546 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5206 0.2020031134709614 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4519 0.17534615247316065 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4463 0.17317324153301972 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3316 0.12866736924120398 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3248 0.12602883452817568 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3062 0.11881166604842176 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2889 0.11209892332262915 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.880198107394571E-5 0.0 18-19 0.0 0.0 0.0 3.880198107394571E-5 0.0 20-21 0.0 0.0 0.0 3.880198107394571E-5 0.0 22-23 0.0 0.0 0.0 9.700495268486427E-5 0.0 24-25 0.0 0.0 0.0 1.5520792429578285E-4 0.0 26-27 0.0 0.0 0.0 1.9400990536972855E-4 0.0 28-29 0.0 0.0 0.0 4.0742080127643E-4 0.0 30-31 0.0 0.0 0.0 8.536435836268057E-4 0.0 32-33 0.0 0.0 0.0 0.001610282214568747 0.0 34-35 0.0 0.0 0.0 0.002328118864436743 0.0 36-37 0.0 0.0 0.0 0.00339517334397025 0.0 38-39 0.0 0.0 0.0 0.004423425842429812 0.0 40-41 0.0 0.0 0.0 0.005800896170554884 0.0 42-43 0.0 0.0 0.0 0.008129015034991628 0.0 44-45 0.0 0.0 0.0 0.014143322101453212 0.0 46-47 0.0 0.0 0.0 0.026327144158672166 0.0 48-49 0.0 0.0 0.0 0.036008238436621626 0.0 50-51 0.0 0.0 0.0 0.04906510506800435 0.0 52-53 0.0 0.0 0.0 0.06441128858274989 0.0 54-55 0.0 0.0 0.0 0.08466592270334955 0.0 56-57 0.0 0.0 0.0 0.1087037499786589 0.0 58-59 0.0 0.0 0.0 0.14300470124802692 0.0 60-61 0.0 0.0 0.0 0.1856286774577563 0.0 62-63 0.0 0.0 0.0 0.23488779243113037 0.0 64-65 0.0 0.0 0.0 0.27382558043883487 0.0 66-67 0.0 0.0 0.0 0.33742202741903193 0.0 68-69 0.0 0.0 0.0 0.41863457380680025 0.0 70-71 0.0 0.0 0.0 0.4737527879223402 1.9400990536972856E-5 72-73 0.0 0.0 0.0 0.5650538493893345 3.880198107394571E-5 74-75 0.0 0.0 0.0 0.6369345193288188 3.880198107394571E-5 76-77 0.0 0.0 0.0 0.7035769218233205 3.880198107394571E-5 78-79 0.0 0.0 0.0 0.7957510278644786 3.880198107394571E-5 80-81 0.0 0.0 0.0 0.8894384111675206 3.880198107394571E-5 82-83 3.880198107394571E-5 0.0 0.0 1.0109856168816556 3.880198107394571E-5 84-85 3.880198107394571E-5 0.0 0.0 1.1747881799853173 3.880198107394571E-5 86-87 3.880198107394571E-5 0.0 0.0 1.3998396702142024 5.820297161091857E-5 88-89 3.880198107394571E-5 0.0 0.0 1.67531433484868 7.760396214789142E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 13125 0.0 25.845 1 TACATGG 13465 0.0 24.976816 2 ACATGGG 13380 0.0 24.599138 3 CATGGGG 6005 0.0 20.957619 4 GAGTACT 10340 0.0 20.828472 12-13 AGTACTT 10560 0.0 20.439516 12-13 GTAAGGT 860 0.0 20.43207 4 GTGTACG 210 8.600218E-7 20.361082 1 TAAGGTG 1070 0.0 19.972748 5 CATGGGA 6630 0.0 19.626646 4 GTACTTT 10940 0.0 19.512505 14-15 AGAGTAC 18565 0.0 18.584091 10-11 TACATCG 230 2.0637144E-6 18.58334 5 GTATAGA 570 0.0 18.336882 1 GGTAAGG 810 0.0 18.176886 3 TCGCGCT 620 0.0 18.00699 60-61 CATGGGT 2110 0.0 17.7809 4 GTAAGCG 295 0.0 17.720621 94-95 CATACGT 135 0.009414021 17.589169 4 TACTTTT 12080 0.0 17.494184 14-15 >>END_MODULE