FastQCFastQC Report
Thu 26 May 2016
SRR936743_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936743_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12936
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT760.5875077303648732No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT650.5024737167594311No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT610.471552257266543No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.39424860853432286No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT360.27829313543599254No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.1855287569573284No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT210.16233766233766234No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA170.13141620284477426No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.12368583797155226No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT150.11595547309833025No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.11595547309833025No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA150.11595547309833025No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC140.10822510822510822No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.10822510822510822No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC140.10822510822510822No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA130.1004947433518862No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT130.1004947433518862No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGA700.00810906927.1317834
GTACATG1108.808983E-525.8985211
TACATGG1102.7523432E-525.8985212
AGTACTT559.154883E-525.89852112-13
GTATCAA1709.582982E-825.1367971
ACATGGG1252.0984444E-422.7906993
AGAGTAC1800.022.42140410-11
AGTACAT904.870839E-621.10249712-13
TCAACGC2107.3668525E-720.3488374
TATCAAC2107.3668525E-720.3488372
ACGCAGA2159.2360096E-719.8756087
AACGCAG2201.1516931E-619.4238916
GCAGAGT2201.1516931E-619.4238919
CGCAGAG2201.1516931E-619.4238918
ATCAACG2201.1516931E-619.4238912
CAACGCA2201.1516931E-619.4238915
GGTATCA1250.005734247618.992251
CAGAGTA2052.3755001E-618.5290229
TACTTTT800.001151880717.80523314-15
ACTTTTT850.001729216416.75786416-17
Produced by FastQC (version 0.11.5)