FastQCFastQC Report
Thu 26 May 2016
SRR936743_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936743_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12936
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1000.7730364873222016No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT840.6493506493506493No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT590.4560915275200989No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.39424860853432286No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT420.3246753246753247No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA290.22418058132343846No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.2009894867037724No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA250.1932591218305504No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT220.17006802721088435No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT220.17006802721088435No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.1468769325912183No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.1468769325912183No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT190.1468769325912183No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT180.13914656771799627RNA PCR Primer, Index 33 (95% over 22bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.13141620284477426No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC170.13141620284477426No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT170.13141620284477426RNA PCR Primer, Index 33 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT130.1004947433518862No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC130.1004947433518862No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT130.1004947433518862RNA PCR Primer, Index 26 (100% over 22bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTGGG200.002027754471.257
GGTAGAG250.00490975657.02
AGTACTT753.0788215E-828.49999812-13
GTACTTT805.761285E-826.71874814-15
GAGTACT805.761285E-826.71874812-13
ACTTTTT906.0736056E-926.38888716-17
ACATGGG1456.924529E-726.2068963
GTACATG1456.924529E-726.2068961
TACATGG1456.924529E-726.2068962
TACTTTT851.0367694E-725.14705814-15
GGTATCA1401.6093394E-523.7499981
CATGGGA1050.002081534422.6190474
GTATCAA1804.5476536E-621.111111
AGTACAT1150.003530252320.6521742
AACGCAG2201.147193E-619.4318186
TCAACGC2001.1317652E-519.04
TGCCGTC757.4452144E-418.99999842-43
TATGCCG757.4452144E-418.99999840-41
CCGTCTT757.4452144E-418.99999844-45
TCTGCTT757.4452144E-418.99999850-51
Produced by FastQC (version 0.11.5)