FastQCFastQC Report
Thu 26 May 2016
SRR936742_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936742_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13683
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT960.7016005262003947No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680.4969670393919462No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT490.3581086019147848No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.30695023021267265No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT240.17540013155009868No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT220.16078345392092377No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.12424175984798655No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA160.11693342103339911No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.10962508221881166No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.10962508221881166No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.10962508221881166No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA150.10962508221881166No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT140.10231674340422423No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA140.10231674340422423No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA804.2572618E-429.698991
GTATCAA1153.4984114E-628.9242321
GTACATG1000.001567871123.7591921
AGTACAT901.8029641E-723.75919212-13
TCAACGC1452.1165413E-522.9399094
TATCAAC1452.1165413E-522.9399092
ATCAACG1452.1165413E-522.9399093
TACATGG1057.962444E-722.6278022
AACGCAG1502.7509308E-522.1752456
CAACGCA1502.7509308E-522.1752455
ACATGGG1200.00451988719.7993263
ACGCAGA1707.221245E-519.5663937
CGCAGAG1707.221245E-519.5663938
AGAGTAC1352.053639E-819.3593410-11
CATGGGA800.001147944717.81939316-17
GAGTACA1053.8155995E-415.83946112-13
GCAGAGT1900.00349712615.0058049
CAGAGTA1805.4667E-714.519505510-11
Produced by FastQC (version 0.11.5)