FastQCFastQC Report
Thu 26 May 2016
SRR936742_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936742_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13683
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT950.6942921873858072No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT710.5188920558357085No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT570.4165753124314843No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.4019586348023094No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT420.30695023021267265No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.19001680917927355No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT260.19001680917927355No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT250.18270847036468613RNA PCR Primer, Index 33 (95% over 22bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA240.17540013155009868No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA210.15347511510633632No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.14616677629174887No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.14616677629174887No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT200.14616677629174887No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.13885843747716142No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.11693342103339911No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG140.10231674340422423RNA PCR Primer, Index 33 (96% over 29bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGGG1254.8530637E-934.1868133
GGTATCA1803.6379788E-1232.0061721
GTACATG1251.7301863E-730.7259251
TACATGG1251.908993E-730.3882772
GTATCAA2001.8189894E-1128.4890122
ATCAACG2154.3655746E-1126.5014064
TCAACGC2206.002665E-1125.8991015
CAACGCA2257.8216544E-1125.3235656
AACGCAG2301.03682396E-1024.7730527
TATCAAC2351.364242E-1024.2459663
GTACTTT1053.158675E-822.61032714-15
GAGTACT1053.158675E-822.61032712-13
ACTTTTT1105.070433E-821.62218516-17
AGAGTAC1901.0162585E-619.9922895
CGCAGAG2651.4942998E-819.709389
ACGCAGA2701.8528226E-819.344398
AGTACTT1051.8508485E-518.088267
TACTTTT1153.980515E-516.54567116-17
GCAGAGT2755.3990825E-615.539463
CAGAGTA2903.5106495E-1014.7356954
Produced by FastQC (version 0.11.5)