FastQCFastQC Report
Thu 26 May 2016
SRR936741_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936741_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12922
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT800.6190992106485064No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT750.5804055099829748No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.4643244079863798No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.3559820461228912No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT270.2089459835938709No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250.19346850332765828No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230.1779910230614456No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.1547748026621266No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA190.1470360625290203No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA170.13155858226280762No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA160.12381984212970129No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC140.10834236186348861No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT130.1006036217303823No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT1300.029.23076612-13
GTACTTT1350.028.14814814-15
ACTTTTT1500.025.33333216-17
AGTACAT550.002802645321.5909112-13
TACATGG600.004640710519.79166814-15
GGTATCA2251.4231391E-619.01
AGTACTT1306.1342816E-616.44230712-13
GTATCAA2656.791848E-616.1320741
TACTTTT1451.72839E-514.74137914-15
TATCAAC2951.8786137E-514.4915252
ATCAACG3002.2021408E-514.2499993
TCAACGC3052.5742238E-514.0163934
CAACGCA3052.5742238E-514.0163935
AACGCAG3204.047579E-513.3593746
CAGAGTA3251.0913936E-1113.15384610-11
GCAGAGT3254.6832276E-513.1538469
CGCAGAG3254.6832276E-513.1538468
AGAGTAC2551.1164957E-813.03921610-11
ACGCAGA3305.405892E-512.9545467
CTTTTTT2003.5074845E-410.687516-17
Produced by FastQC (version 0.11.5)