FastQCFastQC Report
Thu 26 May 2016
SRR936741_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936741_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12922
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1120.8667388949079089No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT840.6500541711809318No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670.5184955889181242No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.4798018882525925No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.2553784243925089No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT290.22442346386008358No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA230.1779910230614456No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT230.1779910230614456No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA220.17025228292833927No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT200.1547748026621266RNA PCR Primer, Index 33 (95% over 22bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.1470360625290203No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT180.13929732239591394No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.13155858226280762No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.13155858226280762No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC140.10834236186348861No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG130.1006036217303823RNA PCR Primer, Index 33 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA2109.968062E-1024.8809531
GTACTTT1000.001566979323.751
TACTTTT1002.6315023E-423.752
ATCAACG2404.6820787E-921.7708343
TCAACGC2455.9408194E-921.3265324
CAACGCA2455.9408194E-921.3265325
AGTACTT804.7513055E-520.7812512-13
ACTTTTT1150.003530252320.6521743
AACGCAG2559.426003E-920.4901966
TATCAAC2559.426003E-920.4901962
GCAGAGT2701.8204446E-819.351859
AGAGTAC2000.019.010-11
ACGCAGA2752.2479071E-818.9999987
GAGTACT757.4452144E-418.99999812-13
CGCAGAG2752.2479071E-818.9999988
AGTACAT1053.8172444E-418.0952387
GTACATG1100.0095768517.2727281
ACATGGG900.002526810215.8333333
TACATGG1250.001431066415.2000012
CTTCTGC950.00362315415.048-49
Produced by FastQC (version 0.11.5)