FastQCFastQC Report
Thu 26 May 2016
SRR936740_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936740_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13214
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT870.6583926138943544No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680.5146057212047828No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT590.44649614045709096No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA500.37838655970939916No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT290.2194642046314515No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.15892235507794764No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA180.13621916149538368No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.1286514303011957No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA170.1286514303011957No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT170.1286514303011957No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT170.1286514303011957No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.12108369910700773No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT140.10594823671863177No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC140.10594823671863177No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1051.6370905E-1145.1931
TCAACGC1207.2759576E-1139.5438774
ATCAACG1207.2759576E-1139.5438773
AACGCAG1251.10958354E-1037.9621246
CAACGCA1251.10958354E-1037.9621245
GGTATCA500.001566280737.962121
TATCAAC1301.70985E-1036.502042
AGTACTT657.730705E-932.85183712-13
CGCAGAG1455.511538E-1032.7259648
GCAGAGT1507.930794E-1031.6351019
ACGCAGA1551.1259544E-930.6146147
AGAGTAC1101.8189894E-1228.04020310-11
GTACTTT601.6611656E-423.72632814-15
GAGTACT601.6611656E-423.72632812-13
CAGAGTA1353.45608E-1122.84757410-11
TACTTTT852.9862204E-622.3306614-15
GAGTACA550.00282064821.56938712-13
CTTTTTT1058.050356E-720.33685116-17
ACTTTTT900.002545806615.8175514-15
Produced by FastQC (version 0.11.5)